人類肝臟表達序列標籤f組的製作方法

2023-05-14 19:16:31

專利名稱：人類肝臟表達序列標籤f組的製作方法
技術領域：
本發明涉及生物技術領域，具體地，涉及一類表達序列標籤，尤其涉及一類人類肝臟表達序列標籤。
背景技術：
肝臟是人體內最大的消化腺。也是體內新陳代謝的中心站。據估計，在肝臟中發生的化學反應有500種以上，實驗證明，動物在完全摘除肝臟後即使給予相應的治療，最多也只能生存50多個小時。這說明肝臟是維持生命活動的一個必不可少的重要器官。肝臟的血流量極為豐富，約佔心輸出量的1/4。每分鐘進入肝臟的血流量為1000-1200ml。肝臟的主要功能是進行糖的分解、貯存糖原；參與蛋白質、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防禦機能；製造凝血因子；調節血容量及水電解質平衡；產生熱量等。在胚胎時期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等。現代醫學實驗證明，肝病病毒侵入人體後，並不直接引起肝細胞的損害，只是在肝細胞內吸收營養賴以生存，並在肝細胞內複製、繁殖。其複製病毒的「零部件」如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細胞膜上，引起人體免疫系統對這些抗原物質產生免疫反應，這種反應造成肝細胞的損傷、壞死。免疫反應的強弱決定於肝臟受損程度及臨床症狀輕重。這場由病毒引發的、免疫系統對肝細胞的戰爭，使大約25％的患者的肝臟成為戰火連綿的戰場，肝臟的損傷由此加重。肝病的危害絕不僅僅限於肝臟本身，它還可以引起其它多種疾病。常見的有(1)糖尿病；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎病；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結節性動脈炎；(12)消化性潰瘍；(13)自發性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對患者的身體甚至生命造成危害，而且對患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學等方面都會受到嚴重影響。
生物基因組中可轉錄表達的序列(即基因)僅佔總序列的3-5％，對這部分序列進行測定，將直接導致新基因的發現，並獲取基因組中與產業化關係最為密切的信息。20世紀80年代，高通量的自動測序的出現，使從質粒互補脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫隨機選取許多cDNA克隆和決定來自非載體兩端的幾百個鹼基的DNA序列成為可能。這些短的DNA序列叫做「表達序列標籤」(Expressed Sequence Tags，簡稱ESTs)。表達序列標籤的概念最早是由Adams等在1992年提出來的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics，2，173-179)針對獲得大量信使核糖核酸(mRNA)序列的迫切需要，提出大規模互補脫氧核糖核酸(cDNA)測序的研究戰略。隨後Venter創立了大規模表達序列標籤技術。其基本特徵就是從以質粒為載體，構建完成的目的組織互補脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫中，隨機選擇許多cDNA克隆，利用質粒上攜帶的通用引物對cDNA兩端進行一輪脫氧核糖核酸序列測定，所獲得的來自3』端或5』端的幾百個鹼基的非載體短脫氧核糖核酸(DNA)序列。簡而言之，表達序列標籤是來自表達基因片段3』端或5』端的短脫氧核糖核酸序列，代表一個表達基因的部分轉錄片段。
表達序列標籤可用於新基因克隆、人類基因組圖譜繪製、基因組序列編碼區的確定等。如果一個表達序列標籤在基因組中只出現一次，那麼它可以作為序列標籤位點(STS)。由表達序列標籤構建的物理圖譜叫表達圖或轉錄圖(expression ortranscript map)。利用表達序列標籤進行基因圖製作，可以加快序列標籤位點的製作和新基因的染色體定位。表達序列標籤可以作為基因特異性探針，對組織特異性基因表達的研究具有重要的作用。表達序列標籤還可以進行新基因的遺傳進化關係分析。表達序列標籤可以對所有動植物的基因作為一種資料庫，通過不同的序列比較可以獲得保守序列片段，從而獲得基因的遺傳進化圖譜。正因為表達序列標籤具有如此的優越性，因此表達序列標籤測序已經成為許多基因組研究機構的工作重點。
由於本發明人類肝臟表達基因與一些肝臟疾病相關，因此，研究人類肝臟中表達的表達序列標籤對探索肝臟疾病的發病機理及研製肝病的治療藥物具有重要意義。

發明內容
本發明要解決的技術問題是提供一類人類肝臟表達序列標籤。
本發明要解決的技術問題通過如下技術方案實現本發明提供一類人類肝臟表達序列標籤的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.50所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
本發明還提供了一種探針分子，所述的探針分子含有上述序列中約8-100個連續的核苷酸。
由本發明的在人類肝臟中表達的表達序列標籤，可以方便的尋找出在人類肝臟中表達的相關基因，從而在研究肝臟疾病的致病機理以及開發治療肝臟疾病的藥物中發揮重要作用。
具體實施例方式
下面結合具體實施例，進一步闡述本發明。應理解，這些實施例僅用於說明本發明而不是限制本發明的範圍。下列實施例中未註明具體條件的實驗方法，通常按照常規條件如Sambrook等人，分子克隆實驗室手冊(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照製造廠商所建議的條件。
實施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源於5個成年男性，在肝臟切除手術後，將肝臟組織立即置於液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩後，再移入玻璃勻漿器內，勻漿後移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑑定總RNA質量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實施例2cDNA文庫的構建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補平末端後，加含EcoRI切點的接頭。磷酸化EcoRI末端後，用XhoI限制性內切酶消化1.5小時，再進行片斷分離。過柱篩選長度＞500bp的片段，用酚-氯仿抽提，乙醇沉澱，無菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進行包裝，宿主菌使用XL 1 Blue MRF』(Strategene，CA9203，USA)細菌。塗板並測定滴度。
實施例3測序及資料庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆，擴增後抽提質粒(Qiagen Germany)，用T3和T7作為3』和5』端的通用引物，採用終止物螢光標記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測序儀(Perkin-Elmer，USA)上進行EST大規模測序。測序結果用FACTURA軟體去除載體序列，傳輸到SUN Ultra 450Server上進行下一步的處理。所有的序列信息再用GCG軟體包(Wisconsin group，USA)中的BLAST和FASTA軟體搜索已有的資料庫(Genebank+EMBL)，將無同源性或同源性低於95％的序列視為新基因建立資料庫。
實施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎上，進行cDNA全長克隆，分兩階段進行(1)「電子克隆」(Electronic Cloning)以新基因片段序列作為探針搜尋dbEST資料庫，將重疊序列＞50bp，同源性在98％以上的表達序列標籤(Expressed Sequence Tag，簡稱「EST」)序列認為同一序列(Consensus Sequence)，取出並用AUTOASSEMBLER軟體進行連接，部分EST可以延伸探針序列。再用STRIDER軟體分析被延伸的序列是否具有完整的開放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和胺基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法，通常可獲取人肝臟相關基因的全長序列。
(2)cDNA末端快速擴增(Rapid Amplification of cDNA Ends，RACE)如果通過「電子克隆」方法仍未得到完整的cDNA全長，則在已有序列5』或3』端設計引物，在人類肝臟Marathon-Ready cDNA文庫(clontech Lab，Inc，USA)中進行長距離PCR反應。然後對PCR產物克隆、測序。用AUTOASSEMBLER及STRIDER軟體分析被延長的序列有無完整的ORF，如無，重複上述過程直至獲得全長。
(3)RT-PCR對於5』和3』端的已知的序列，如果中間有一段間隙(gap)無法從已有的公共資料庫或自身資料庫獲得，可考慮採用RT-PCR的方法。在序列5』端設計引物，3』端引物採用Oligo-dT，在肝臟總RNA庫中進行擴增。然後對產物進行克隆、測序。最後拼接便獲得全長。
通過組合使用上述3種方法，可獲得人肝臟相關蛋白的全長編碼序列。
序列表110上海人類基因組研究中心120人類肝臟表達序列標籤F組130NP-10044160502101211562212DNA213Homo sapiens40011 gaaaaagtat attcaataaa ttttgtcttt aaaacagagc tcttgaccta taaagtataa61 aaagtaatta caatgaaata ttcttcagta aatctgacac tttgggattc caggcaaaag121 gatcgcttgg gtgccaagag ttcaagacca gcctggtcaa catagtgaga ttctatctct181 gactctgcag ttcactctgc tgcttcagat gaaaattttc aggtctgtct gccactgtag241 tgaaggactg ctttgggtag tgtctgtgga gaaacttttt aaaggacata gttaaaatat301 tgtgctacaa accaattctt gaacaccaat cgttttgtcc tacctctctg tgacagtgaa361 agatggctgt gctcttggca aaggtcatct ctctggtcat gattctggat ctccttttct421 tctcctgatt atatgtcact cagttatccc cactggtctt ccacaacctt taacctcagc481 ttccccaatc ctaggagact cttctcgcag gctcnaggct ctcctaccct cccatcaatc541 ctcaatctac cccaaaaagc ca2102211437212DNA213Homo sapiens40021 acctgccctg acctccgaca gtgctgggat tacaggcata gcacccgtgc ctgaccaggg61 ctcttttagc aaggaaaacg tgaggaatga atggctgttg gtgtgcaaca aatcatactt121 gctacatgtt gtgaaacctg aagttatttg ttagtctgta tgaagaatgt accccagaga181 tgcaccctgt atctgcctta tgtctcttac aatggaggtt ctctcctgtt atattgctga241 ataaactatg tgaactgcta aattgactga agactagtta gtttatcact aactctttaa301 tgaagaattt cactatccta caactcctcc taaaaagtga aaatttactt tngagagagt361 gctttcgaga atagtattat gatcagtatt attgccccta aattttttaa ttattagaga421 atgcctnatg ttacaag
2103211590212DNA213Homo sapiens40031 catatacttt atgctttact tcttacttca aacatacatc cttgattaaa gaacaaaaac61 aactcactga agggtgataa aataatcaaa atgtatttgc ccctgaaagt ggaattacta121 cctcaaaaag aatacaactc tttcattttc cccaaaataa tcatgtgagt tcatgggcat181 gctcatcact cgctgtctgt gtggaagaga agatcgaaga gggatttact ggactgaact241 ggcctaggaa gcctttgctg gcatctctca gactggactg cagcccagat ccttttactc301 agatgcatgc acttagaaca tgaaaacant aagataaacg ctggtggtat tattacctta361 tattgaggaa cattttatga cttcctaact ctgntttcaa tagaaacatc cccacttaat421 gaaattgtca ataaatgctg gctcaaacca ccctccccaa atactggaaa aacaggtaca481 tccggtttct cnggtacccc tggncaagtt gtctggcaat ggcttggcga tttttcnact541 gaggttagac aacttgngat ttttttcccc ttcttnaaca cnaatttaaa2104211587212DNA213Homo sapiens40041 ttttaaaggg gaaaaatctt taattgattt ctcaggtaat ttttttccag attgtacata61 aagtgttctt atgttctcta tttggatgtt tcaggagaca tacaaatgaa atacagtaca121 tagacaaatg aaatgctaat aagaagtagg atngatatta aaatatcctt actttgcctg181 tatggaacaa aggcagtcta ctccatcggg gaatcaaagc aaatgtgaat aagaggtttc241 caccttgcaa aactgtgagc ttcatttgcc ctggagagaa ctactaggca aggcttcaca301 tgacagtacc tngtagggat gtccatgggg ctgtgaagca ggtgtattgt atccgaacat361 gcagctgcaa tgaaggcgtg gagctcagga gaagcagcca ctgacaatgc cccctcctgg421 ccgcagggtc ttctgtgcag cctcccatgg atcaatatac ccagttagtt aaaacaccca481 tccagtantt tctggtccct tagcaaccga tggatgatan ttgccnccag ccgagtgtga541 tcngtgangc cgattttaaa agcagncccg gggttcnacc cggantg2105211508212DNA213Homo sapiens40051 tttttttttt ttttttatga tttcacaaat cacatggttt attgccctac agagatgata61 tttttcctag gttcacaatg cttanggagg gggagaacaa catgaacaaa tctcacaggg
121 aatgcgctgg gtagacaggg tgtattagtc acggggtgag tcgagttcca aagttactaa181 agtctacttg ctcaattagg atggaagcag gcctctgtgg agagcgaanc tcattaaaat241 gtggtgtgtc tcttttataa gagcagctct tgggagagaa gctgtccaat gcaacggaga301 ttcacagaga catatatngg tccctcaagg ctagagggtg gcactcggtg tttgtgggcg361 atgcttccaa gttcaaaggc ttagcagggt ggggccctgt cnaagagagg ctgacagagt421 gttagcttgc cacagcccaa gctctcaaag tgtctggtcg aaaccntaag gttttttaca481 aaagttttgn aagtttcagg tgggtttg2106211564212DNA213Homo sapiens40061 gttttgtctt cctttattac atttttgctt ttgagataca aaccaacctc tcataccaca61 caaagaacat ccattcttgg gatttttaaa gtgcatttcc ccttgaactc tgtgtacaaa121 aatatttatc ttttaaaaca tgcaaaaatt tcttgacaag gcacttttag gtataaaatg181 aagatgagtc cttggttcta cattcacact gaagtaatag tgaaacatca tcacagctgc241 actctcaaag ccctcagagg tccagcagtc tctaaaaact cgtcaacaag actaaaaaca301 ttcacggctt tacaatgtgg gttacagagc tttacaacca tggaccagga aaaactgctc361 gtaacaacag ctgtccttcc cagttccaca tggtgttggt ctcactggca gggaaattaa421 ggataagctg gccaaattgg tcnttggtgg tggaccccaa taagantccc atcatcccat481 ggtnaccttc ctggtccccc ganttccagt ggacatggtc aggagagacg ttgatcgggg541 actcccccag gnctacattc aggg2107211467212DNA213Homo sapiens40071 tttcataaag aaaagaggtt tatttggctc atgattctgg tcgctggaaa gtccaagact61 gggcagttgc atctggtgag ggactcacat tgctttgact catggtggac aggggacggg121 gagccagcat taatatagaa gagcagggnt cccctgcctt gaggaggacc aatgcaggaa181 aggccaaaga gcagttagaa atcagcatcc cgagagantg caagatnttn tgaccaaggt241 ntctaantat caaacgcaag tcaggcggta atgacttctc ggagggagct ggcttcggct301 caggccnttc cgggtggggg gagaggaaac tgaagacaaa actntgcttc tcgtaaaggt361 ctatttccta aaattaaaga cagagttagc ctagtgagtt tctgcaggga tgcttgtctn421 agttagagaa gttaccagag ccntgccnag gttcttgagt tagggca2108
211482212DNA213Homo sapiens40081 tttaatcagg tttttcacta tacagtgatg ggggtagaag atggtgactg ttttcaagca61 aattcacgag gcacatgttc gtccctgccc caaagtgaac agtctgggct tcccagaaca121 gaaaagtgct ttccttcctg ggggaatccc attcctgagc tgacaagaca gatttcagta181 agaatgagca caaaggatag ggcaaaatag tgaagggagc caggtgcata tttgaattct241 ttccagtgca gactggaact agacatgcag gtatccctcc taaaggcaac gccaaatccc301 agccattccc actagaggcc aaaccgcctg cccacagaga ttgacagcca atgttcatct361 cataactntc ctcccagcag tgcaccagta aactcaggtn gcctgagtgc tttntgggca421 ccacacaaca gntgcgggcn ttctnttcat tgggccctng ggtgctgntg gggtntgctt481 gg2109211348212DNA213Homo sapiens40091 nntaatanta antagacagn ctttattnng nanantnagn nnnagnncaa nagnanctat61 anngagtnca taaaagcttg tttttaatta cttataacaa agcacaaatt acttctaaca121 aggcaaatat ttattgacat ataagacatt ctgctaggca ttgtaaaaan nnnnnnnnnn181 aagtaatggc ttttgtatac ataggtactt aaggcagnca tccnaangng ggnaaaatgc241 ttaatgagtg anggtagcnc tgnnncntgt tgacttttac tctagggttt gtttttaggt301 gggggttagg tnttttcccc tacggtantg tacntgtctt taggcccc210102111240212DNA213Homo sapiens400101 acatacatcg ggtgtgagca cggaatagct gggcggcggc tggtccacag acactgggag61 acagaagggg ccggtcccca ggcgcccgtg ctgcagcagt gggatccact ggggagaggc121 tggagaccac cggtgtgaag gcctcgcggg taaattcact gcagctggac ttgccaaaga181 tgacctggga gagtggattc atgagggacc catgggggat gccctacccc ccttccctgg241 ggcacagcca ctgaccggca gagcctggct ggggtcctcg cctgtcatgt actgcactcg301 cacggcaagg ttgcgcacgg agccctggcg gctgctgaag ttgaggctgt tataccttag361 accctcagtc atgccagtgc ctgctctgtg cctgctctgg gccctggcaa tggtgacccg421 gcctgcctca gcggccccca tgggcggccc agaactggca cagcatgagg agctgaccct
481 gctcttccat gggaccctgc agctgggcca ggccctcaac ggtgtgtaca ggaccacgga541 gggacggctg acaaaggcca ggaacagcct gggtctctat ggccgcacaa tagaactcct601 ggggcaggag gtcagccggg gccgggatgc agcccaggaa cttcgggcaa gcctgttgga661 gactcagatg gaggaggata ttctgcagct gcaggcagag gccacagctg aggtgctggg721 ggaggtggcc caggcacaga aggtgctacg ggacagcgtg cagcggctag aagtccagct781 gaggagcgcc tggctgggcc ctgcctaccg agaatttgag gtcttaaagg ctcacgctga841 caagcagagc cacatcctat gggccctcac aggccacgtg cagcggcaga ggcgggagat901 ggtggcacag cagcatcggc tgcgacagat ccaggagaga ctccacacag cggcgctccc961 agcctgaatc tgcctggatg gaactgagga ccaatcatgc tgcaaggaac acttccacgc1021 cccgtgaggc ccctgtgcag ggaggagctg cctgttcact gggatcagcc agggcgccgg1081 gccccacttc tgagcacaga gcagagacag acgcaggcgg ggacaaaggc agaggatgta1141 gccccattgg ggaggggtgg aggaaggaca tgtacccttt catgcctaca cacccctcat1201 taaagcagag tcgtggcatc tcaaaaaaaa aaaaaaaaaa210112111109212DNA213Homo sapiens400111 actggaagac caggcagccc agctgaaggc agtaagctcg gctcacagtc gcaggagagt61 tctggggtac acgggcaaag gggcttgaga aggcccggag gcgaagccga agagaagcaa121 ctgtgccccg gagaagagaa gctcgcccat tccagactgg gaaccagctt tcagtgaaga181 tggcagggcc agaactgttg ctcgactcca acatccgcct ctgggtggtc ctacccatcg241 ttatcatcac tttcttcgta ggcatgatcc gccactacgt gtccatcctg ctgcagagcg301 acaagaagct cacccaggaa caagtatctg acagtcaagt cctaattcga agcagagtcc361 tcagggaaaa tggaaaatac attcccaaac agtctttctt gacacgaaaa tattatttca421 acaacccaga ggatggattt ttcaaaaaaa ctaaacggaa ggtagtgcca ccttctccta481 tgactgatcc tactatgttg acagacatga tgaaagggaa tgtaacaaat gtcctcccta541 tgattcttat tggtggatgg atcaacatga cattctcagg ctttgtcaca accaaggtcc601 catttccact gaccctccgt tttaagccta tgttacagca aggaatcgag ctactcacat661 tagatgcatc ctgggtgagt tctgcatcct ggtacttcct caatgtattt gggcttcgga721 gcatttactc tctgattctg ggccaagata atgccgctga ccaatcacga atgatgcagg781 agcagatgac gggagcagcc atggccatgc ccgcagacac aaacaaagct ttcaagacag841 agtgggaagc tttggagctg acggatcacc agtgggcact agatgatgtc gaagaagagc901 tcatggccaa agacctccac ttcgaaggca tgttcaaaaa ggaattacag acctctattt961 tttgaagacc gagcagggat tagctgtgtc aggaacttgg agttgcactt aaccttgtaa1021 ctttgtttgg agctggcacc tcttgaaata aaaaggagga tgcacgagct ggcaggcatg1081 caaaaaaaaa aaaaaaaaaa aaaaaaaaa21012211476
212DNA213Homo sapiens400121 tcatttttat tacaccaaat taaagttggg agttccaaga tcccattcca gttaatattt61 aaccaaggtc taaaatttga ttttttttaa atctttgaat cctcccttct gcccctcatg121 gatccttggt tttaatgata tggaaacatc taattcttag aattattccg tagcttttgc181 tgattactct gagatttcag ttaagacttg tttcaaaaga cagatagctg actggttcat241 aatacattgg aataagttgg atccaaacta ataagaataa gctgtacagg aactaagtgc301 tcaatataca ttgtataaat ttgtggnaat ctcctggatg tgaattgtta cttcaagtgg361 cctttattaa gattttctca gacttacctt gggaggttaa agcaaacccc aatgnggaat421 aattttggtt acagagcnct gcctttataa ttttggaata aaggttcaat acagac21013211500212DNA213Homo sapiens400131 ctgtctgaat caactgtacg agttaaacag ttttatttcg aaattaacca acataaacaa61 caacaacaac aacaacattc actacccatt ccctcgttag gttggaaaac cagtctgaaa121 aaataaatac aatctgtgac catgaaaagg agcaatcact tatttttccc atatgttttg181 aacattattt ttaaaaatag attgggaatc tggagggtaa aatgccgggt cccttcccga241 gcccttagag cccgaacgtt gtcgaatcgg tcaatgtcca ccccgctgct cacctctgtc301 ccagcagcgc cggggccagc gcgccctccc gccgcgtctg ggagctggcg gggaaaagca361 ggtccccggg gggtatcgag tgtccaggga tatccacgca gacatccttg tacttgcaaa421 tacaaagaaa cacacaggac agagatcagg ctcgcgcagg gctcgatctc ctcggggctc481 agggttggag ggccgtacan21014211474212DNA213Homo sapiens400141 gagacctttg tggatgcact caggatacat tcctccttgc cccatcccct ttgctgcctc61 ttattcattc aacaaatatc ctgaaggatg agaaaaaggc taggcttgca gagtgctatg121 gatacaggaa acagtatata aaaaagttcc agatggtggc tcacacctgt aatcccagca181 ctttgggagg ccgaggtggg cagatcacga ggtcaggagt tcaagaccag actggccaac241 atggtggaac ctgtctctac taaaaataca aaaattagct gggcctggtg gcgggcgcct301 gtaatcccag ctagtcggga ggctgaggca ggagaatcac ttgaacctgg gaggcggagg361 ttncagtgag ccaagatttg agtcactgca ctccagcctg ggtgacagag caagactcca
421 tctcgaaaaa agttccagan gtgggaaaga gcntgctacc ntctgggggc taan21015211611212DNA213Homo sapiens400151 cctttaataa taaaaaagaa aaccaaaacc tcctataatt tataagctat gtttgactat61 ctacattata tagaaaatat agactctgtt tactttataa cacacatctt tttcccttga121 taaataactc tttaaaatat ctagtataca tcgccttgac ttcttatata tataagtttg181 gttttataca tatacataca tatatactca tgtttgtaaa acacaataaa tatatactcg241 acaatgacag ccaaacaaat gccattttgg ttaaaaaaca caacaacaac aataaaaagc301 agatgaagta ctataaaagc accaggcagc agacagaaag ccacatttgc tagaaacttc361 tccctcccct gggcaaggca caggggcaga atactaaata cgtccaggtg cctgaaagag421 aaggaaggca gcaaaggaat gggtcagatc acaagctttt ggttttgttt cttagcctgg481 ggattagacc aagaatcaca agtaagtcat tgcgtttata agggaaaacc aggggctcat541 tcaacagctt agccctgtgg tggctgcngg gacagtagag tctgggaggg ggaaggagag601 agcngtgacc t21016211474212DNA213Homo sapiens400161 gaatttaaat catttatttt cacttattac taatcttact acagagaata atacaatact61 ataatgtatc atcctcagta aattaatctt cacttagaaa atgttactga taaaatacaa121 catttagaat tttcatttaa aaatacaaat tttatgaaaa tattaagaat ttttaggatg181 ggaattataa aagtcaatgc tgaacaaaat tctactttta gatagtatgt atcttaaaat241 attatgaaaa atattcttta ttctgaaaac aatgtcagga aagaatacct gagttctcta301 aaaccactag ttctaatttc aaattcgctg ttttggtaac ataaagaaaa cacatttaca361 atttaacact ggcagttacc tgtcaaataa tttaggaata gaataaatgt tgcatatacn421 ttagggttgg acnttatata tattttaaaa cncaggggtg ggtttcattt taat21017211582212DNA213Homo sapiens40017
1 nagctctatt aagttgcaat tttattttta taaagctttg taaagttatg aacatggaaa61 ccctaagaat cacctaagct ttttacataa gtaaatttca agttgacagt ctgtccattt121 tgaactattt ataattaaca ggacacttaa cacagtaata tcttagacaa ggaagagggc181 attaattctg caggcaggta gcaatgaaaa tgccaaacct gaacaatcag tcactatttc241 cttatacagt ttgagtaaaa gaggtagact acagaaagaa aaaaaaaagt gaatataaaa301 tgtatcttcc ccaaatacaa ctctacttca ataagtcttt gaagattacc ggatgacatc361 caacaaaaat ggaatggaac tcaggggaat ggagttcttt ctccccatcc aaaggaaaaa421 aacagcatgg ggtaattttt naatttggaa aaanaaaaga atgggcattt ggcaagcnaa481 ggaaancccg nttcccattc cacctatttn cattggtcca ttcccngggg ccaaaatnaa541 tattnccttn ggtaatttaa aaaaaaancc cttttttaaa cc21018211600212DNA213Homo sapiens400181 aacttattta tagaaatgag gtctcactat gttgcccagg ctggtctcat acttctggcc61 tcaagtgttc ctcctctttg gcctcccaaa gcactgggat tacaggtgtg agccaccatg121 ctttggcatg nggngctatc gcagtaggag agcaggggtg gtggtagtgt ctccatatgt181 actctcacta aaactggtgt gggcagatac tggatgccta cttatttagg ggcatctaaa241 ttcctgtgct aaattagaaa atagaggcag ctcttgaaag gagtgatggc atgaaggaga301 gatgcttgga ccaaccacca cacaaccctt ataaataatt gataaccttg gtttttgatg361 ccacgtcctc ttcaacagtt gttggttgaa atgacacatg attccaggtg agcttttcca421 gataaattgc cctgggaact tcagactttg tcgggtgaat cgatgtggaa tttcagaatg481 ggctggaatg tctaagatgg gcatgtccat attcagctca gtgtggggaa actggtggtg541 gccagggctg gttgcaagct gagttgtaag ggtgacagtt caccattggt tatttcnggg21019211598212DNA213Homo sapiens400191 tgataggcaa aaggttttaa ttgtatagat taaaattaac tttggacaaa aattaaaact61 caggcagaga atgtcttctt tttgcaacag cagacactag taaaaacaaa ggcacagtaa121 aaattgagac cccaaatttg cagtgtagag atatgaatat aataatagac acaggcaggg181 aggattaata aatgataaaa tgtttagagg atgatcatta gaatacagga tatttatact241 cttgaaaacc gctttcccaa gtacttcatt ataagtaagg tgtctctaaa agggacagat301 ctcctagacc cctccttaac caagtaacca gtcctgatat cataatggtg atggacaaac361 tagaccttct ctgcccgcag atgggctgag gttggaaact cacagcattg tctctgcagt421 gttcctgggc aaaacgttta ggctgaattt aatcatgaag acattttcag acaacttcag
481 aatggtagat cnctggagcc agacagctga cctggtcttc cataaaccag tccatgtcac541 caccatccnt ggaccaccac caaaagatga ggaattttgg ggttccaaat actaagga21020211534212DNA213Homo sapiens400201 aggggtgtat ggttttattt ttttaaaaat gtatatgctc aataacagtt tacttggaaa61 ttttcactag aagaaataac atgaaagaac accagcataa atacataata catactttaa121 ttgtggctat attaaatgta tcttaatgta ataccgctgt aacatggtat catctcattg181 ttaaaaatta aaaaaaaaaa aagaacatag ggaagtgaaa ctaaaaaact atagaatcct241 ggattctgtg gcagatggag tacccttatt taagctagaa aaggagccct gtaaggtaga301 aagagaaaag aggcaagcaa tctgaaacca tcatgttaac caactgtgag tttttaaaaa361 tgtggttaaa atgaatctaa gaatgccttt taacacagct cacactttaa tgaacaccag421 agaattcnca gcaagactgg taaagcctag gaatgtaaag ttattggtgg ggntttagcc481 acgaccctca cctcactcct acctatatgc caaaggagga angaaaagtc ctta21021211547212DNA213Homo sapiens400211 gaaaaataaa catctttatt tttttgccta ctttatttca ttttttcaaa taaaatttaa61 atctgtacaa agtatactgt tacagtatat attttgtaag aatcaatgcc taaaataatc121 acaatacttc aataagcagt acagcagacc tcgctagttt tcagctttga tattgaacaa181 actcaagccg gctgatgcac aacacgtttg cttggtttcc acatggtgat ttcccagcac241 tgagatggga gaacatgaca gcaaatatgg taatattaca gcccgacaca ctgcgtttct301 tcatgtgata ataactgcac atatttaata cagaatgctc aaatttactt tttaaattgc361 atttgcttac ttcttagttg ggcaaaattc agcattctta aatggggtcc ccaaacagtg421 gnaatttaat gntattcnaa ctggtatatt gggcacatca cccnataggg gttacatggt481 aactcncaag gatatcnaca acttagtccc ncaattttta cctaatatcn tctgggncca541 tttaacn210222111231212DNA213Homo sapiens
400221 caaagaggaa atggaggttt tgcctgagcc ccctcctcca ataaatagga aaaaagacaa61 gagctatgct acagctatgg gaccctttct taggcaagag gcattaagtg gggagctctt121 agcctgcctg gtaatacaag attgacaggg caattgggta tataaaccca tttcttttaa181 catttataaa aagttaagaa aaagcattag aggctgaaac cgcgtggcca aatgggtggt241 aggcagaagg aaatacttgg cagcaaagga agctcgcaga cctggagcca gcaagtgctt301 ccaggaactc agcctgcaca gaggcagcag tgacaaggag ccaggcgcag cacaccagag361 ctggcctgat agggaggggc aggaggcgtg cacgaaggtc cgcccagcgg cagcagggtg421 agggagcagc acagacaaaa ggtggcacag acaaaaagca gcgcctgagc aagcgcaaca481 tgaccgccgc cccgggaccc gccggctaga ctctcttggc tccgcgggtg gcccatgggc541 aaaatttcat gtgttccttg tatacaagcg acatcccaga ttataattct ctgctaagat601 ttaagtacaa tttaagaatt taaaacacct ctttctaata atggccactg ttgcttttac661 actattcccc tggcaaagca agacagaaaa atttgcattt acaataccag ctatcaatat721 tgaaaggtca gcttgccgat ttcattggaa agtgcttcct caaggaatgc taaacagtgc781 tgccatgtgt cagtatcatg taaatcaagc tttgttcccc agtagaaaag aatttcctca841 ttgcaaggtt attcatttta tggatgatat tctactagca gccccagtgg agccagtgct901 tttaagttta tatacctctg tcataaagaa tgcacagcta agtggtttaa tcattgcacc961 tgaaaaagca caaatgtcct ctccttggaa gtatcttggg tacatacttc ctggtcagta1021 agacctcaaa aggttaaatt aaatactagc aacttacaca ccttaaatga ttatcagaaa1081 ttactaggta attttaactg gcttcacccc accttgggca ttcctactta taagctgcaa1141 aacctgtttt ctatcttaaa gggtaacaca gccctggatt ctcccagata tttaacccct1201 gcagccaaaa aaaaaaaaaa aaaaaaaaaa a210232111408212DNA213Homo sapiens400231 aagatcaagg actgctacgg actgggctcc gggcagaatc atttcatcaa ggacagtcag61 tgggagcagc aagctgagat cttcaacgct tcctacaaga agtacctaga tagggagtgg121 gaggaagagc cactcagtac ggccaccttc tatttccttc ttcctagctg cctatttgca181 atgccaccgg aagtcaaggg cccctcagga atggcctgtg tccttggtat acactggacc241 agaagtcaca atttcttcct gtattcacta aaccgaactc taaaggataa agctgacccc301 gagggtgtgt ggccctgtgc tgcgcccatt gcagtctctc agctcagctg ctcctcctcc361 tacctggtgc tggcctgcga ggatggtgtg ctcacgctgt gggacctggc caaaggattc421 cctcttgggg tcgctgctct tcctcaggga tgtttctgcc aaagcattca cttcctaaaa481 tatttctcgg tccacaaagg acagaatatg tatcctgaag gtcaagtgaa atcccaaatg541 aaatgtgtgg tgctgtgcac agacgcctcc ctccatctgg tggaggctag cgggacccaa601 ggacccacca tcagtgtgct tgttgagagg cctgtaaagc acctggataa aaccatctgt661 gccgtggccc cagtcccagc cttacctggc atggtgctca tcttttccaa gaatggctct
721 gtgtgcctta tggatgtggc caagcgtgaa atctgtgcct ttgcccctcc gggagccttt781 cctctggagg tcccctggaa gccagtgttt gctgtgtctc cagaccatcc atgtttcctg841 ctccgaggag actattcaca tgaaactgcg tccaccgacg atgctggaat ccaatattct901 gttttctatt ttaattttga ggcctgccca ctcctggaaa atatctcaaa aaattgtacc961 attcctcaaa gggacttgga taacatggcc ttcccccaag cactgccact ggagaagaga1021 tgtgagcgtt tcctccagaa gagctatcgg aagctggaga agaacccaga gaaggaggag1081 gagcactggg cccggcttca gaggtactcc ttgtcgctcc agagagagaa cttcaagaag1141 tgaggctgcc accgccctgg gatctctgaa aaggaggttt cagccacgag gcagctgctc1201 ccaggacact gaggccaaga gaaatgtaac agagccacag ctccacaggc ctgcactcgg1261 agtctggggc ctctgcagag ccagcaaggg gaaaagtata atctggggga ccttcaacca1321 ctaagcctct tgtcagagcc ctcaggcagg cagatgtgtc acccaaataa acagtgatat1381 tgtctccaga aaaaaaaaaa aaaaaaaa210242111026212DNA213Homo sapiens400241 gtttgttcct aaatctaatc gagctcccaa ggaactagta tcttatagaa cacacataga61 aaatagtgct ctacagtagt cactttcaca tttttttctt ggatccaact tcatgataag121 aaatacattt taactcataa tctattcaca tgtatatgaa taactgaaac agaagtttca181 caaaattatg attatgtttg ccacaaatgg tgtgctctaa tattttcctt ctgtttcatc241 agaagaaaaa tactggttac tatccagcta agctgatttt gtgacccatt aatgagtttc301 agttctcact ttgaaaacac tgttctagaa ttacagaaag gtctagggat gagaactaac361 accactgtgc atcacagtgt gtcagtctgt gccaggcagc ttataaatat tttttgcctc421 taatttttat acatttatga ggtaagtatc attttctagg taaggatgct aatctgtctc481 caagccaaat aacacacagt aaatcatggc accaggattt gaatctgggt ctttatacat541 catagcccat gctgttctca ctgtattttg ctttttccaa gtataacccc gttttcacac601 gaatggcccc ttcacatatt tgaagactac cgtcgtgtcc gtgctgaccc tttctccctg661 ccacacatgg ctggagtgca atggcgcgat ctcggctcac tgcaacctct gtctcccagg721 ttcaggaaaa tggctttgta aagaagcttg agcctaaatc tggctggatg acttttctag781 aagttacagg aaagatctgt gaaatgctct tctgtcctga agcaatactg ttgaccagaa841 aggacactcc atattgtgaa accggcctaa tttttctgac tcttacgaaa acgattgcca901 acacatactt ctacttttaa ataaacaact ttgatgatgt aacttgacct tccagagtta961 cagaaatttt gtccctattt aatgaataaa ttgtatgtat ttttaaaaaa aaaaaaaaaa1021 aaaaaa210252111067
212DNA213Homo sapiens400251 atgacctttc ctctttatct tccttgttgt gcaggtaaag aaaccaagtg gaagagtgtt61 tcctcctctg gccgtaaagc agctgtcccc gccctactcc ggaccgcccc aaagactcca121 tgggatggac ctgagtcagc cgaatcctag ccccttccct tgggcctgct gtggtgctcg181 acatcagtga cagacggaag cagcagacca tcaaggctac gggaggcccg gggcgcttgc241 gaagatgaag tttggctgcc tctccttccg gcagccttat gctggctttg tcttaaatgg301 aatcaagact gtggagacgc gctggcgtcc tctgctgagc agccagcgga actgtaccat361 cgccgtccac attgctcaca gggactggga aggcgatgcc tgtcgggagc tgctggtgga421 gagactcggg atgactcctg ctcagattca ggccttgctc aggaaagggg aaaagtttgg481 tcgaggagtg atagcgggac tcgttgacat tggggaaact ttgcaatgcc ccgaagactt541 aactcccgat gaggttgtgg aactagaaaa tcaagctgca ctgaccaacc tgaagcagaa601 gtacctgact gtgatttcaa accccaggtg gttactggag cccataccta ggaaaggagg661 caaggatgta ttccaggtag acatcccaga gcacctgatc cctttggggc atgaagtgtg721 acaagtgtgg gctcctgaaa ggaatgttcc agagaaacca gctaaatcat ggcaccttca781 atttgccatc gtgacgcaga cctgtataaa ttaggttaaa gatgaatttc cactgctttg841 gagagtccca cccactaagc actgtgcatg taaacaggtt cctttgctca gatgaaggaa901 gtagggggtg gggctttcct tgtgtgatgc ctccttaggc acacaggcaa tgtctcaagt961 actttgacct tagggtagaa ggcaaagctg ccagtaaatg tctcagcatt gctgctaatt1021 ttggtcctgc tagtttctgg attgtacaaa taaatgtgtt gtagatg21026211770212DNA213Homo sapiens400261 gctcagcctg ccccatcccc tgctgatttg cctgttccta gagcacagcc ccctgccctg61 aagacttttt ataggctggt cacacccgga gcaggagtca gccccagtca ggacacagca121 cagacatgag ggcccccact cagctcctgg ggctcctggt gctctggctg ccaggtaagg181 aaggagaaca ctaggattat actcggtcag tgtgctcagt actgtctgga acttcaggga241 agtcctctga taacatgatt aattgcgaca atatttgttt ttatgtttcc aacttcaggt301 gccagatgtg acatccagat gacccagtct ccatcctccc tgtctgcatc tgtaggagac361 agagtcacca tcacttgccg ggcgagtcag ggcattagca ataatttaaa ttggtatcag421 cagaaaccag ggaaaactcc taagctcctg atctatgctg cacccagtct gcaaagtggg481 attccctctc ggttcagtga cagtggatct ggggcagatt acactctcac catccgcagc541 ctgcagcctg aagattttgc aacttattag tgtcaacaga gtgacagtac ccctcccaca601 gcgttacaag tcataacata atccccaagg aagcagatgt gtgaggctgg gctgccccaa661 tgctccttct ggtgcctcta tctgctgagg gaagttctca aactcagtca ggtttggaaa721 gtcattggga gattttccta gaggaggcca gggaggttcc tctgaaccct
210272111035212DNA213Homo sapiens400271 gtcacaaggt tgattgatta gttggggtga ggcaggaaca aatcacaaag gtggaatgtc61 atcttttgtg gttcttcagt tgctccaggc catctgggtg tatatgtgca ggtcacaggc121 ttagcttggg ctcagaggcc tgacaattta taaaatactt cattcaacaa tagcagacca181 cacattcttc tcaagttcac atggaacatt tgccatgaca gatcacattc tgggccataa241 aatacacctt agcacctttt caaagaatag aaatcatacc aagtactctt tcagaccaca301 gtagaattaa actagaaata aataaataac aaagttaact ggaaaatccc aaaatatttg361 gagattaaac aaaacactac taaataacac atgaaccaaa gaagtctcaa gaggcataaa421 aatattttaa gctaaacaaa aaatataact tatcaaatct tgtgggatgc aggaaaagca481 gtgcttagag ggaaatttat agtattgact acatagatta gaaaagaaga aagatccaaa541 atctacaagc ttcaacatta ggaaacgtaa caaaaaatta ataatcaaca ttagaacaga601 aattaatgaa attgaaaaca ggaaatcaga gaaaatttta aacatcaaaa gctggttctt661 tgaaaaaaaa aatcaataaa atcaataaaa ctttagccag gctaaacaag aggaaaagaa721 aagagacaca aattccaaac atcagaaata aaggaggggc atcactactg gtaacatggc781 taataaaaag attaaaagga atatcatgaa cagccctaca cccataaact tggtaactta841 gataacattg gccagtttct tgaaaaacgc tatctaccaa aactcaaaca aggagaaata901 taatctgggt gggcctacat caattaaaag aaatggaatc cataattaat actcttccaa961 aacggaaagc acaagactca gatgttttca ctggtgaatt ctaccaaaca tttaaggaaa1021 aaaaaaaaaa aaaaa210282111057212DNA213Homo sapiens400281 acgctgccca gactcactac ccgccctctc ggtcccgcag ccacgaggag gacgctgccc61 agactcacta cccgccggct ccctcccccg cgtccctgtg gtggtgggcg aagatgacct121 gttgtgcatc agatgccttt gagacataca actggagatg tgagatagga aattgaatgt181 aaagactgga agctcggcag agcagaactc tgaacaacgt gcctcaggag tgcaagttgc241 tgatgaagtg tgtcgcattt tttatgacat gagagttcgt aaatgctcca caccagaaga301 aatcaagaaa agaacaaagg ctgtcatttt tttgtctcag tgcagacaaa aagtgcatca361 tgtagaaggc aaagagatct tggttggaga tgttggtgta actataagtg agcctttcaa421 gcattttgtg ggaatgcttc ctgaaaaaga ctgttgctat gctttgtatg atgcaagctt481 tgaaacaaaa gaatccagaa gaattgatgt tttcttgtgg gcatcagaac tagcaccttt541 gaaaagtaaa atgatctata caagctccaa ggatgcaatc aaaaagaaat ttcaaggcat601 aaaacatgaa tggcaaacaa acggaccaga agatctcaat cgggcttgta ctgctgaaaa
661 gttaggtgga tccttttttt tttttttgag atggagtctt gatctgtcgc ccaggctgga721 gtgcagtgac aggatctcgg ctcactgcaa cctctgcctc ccgggttcaa gtgattttcc781 tgcccagcct cctgagtagc tgggattata ggcgtgcgcc accacacctg gctaattttt841 gtattcttag tagagtcagg gtttcaccat attggtcagg ctggtctcga actcctgacc901 tcgtgatttg ccctccttgg cctcccaaag tgctgggatt acaggcgtga gccagcatgc961 ctggccagtg gatccttaat tgtagccttt gaaggatgcc ctgtgtagat catcattcag1021 tgccacaaat tgaaagcttc cacgtttaat gttatcc210292111003212DNA213Homo sapiens400291 acgcgtccgg aaatgtctta cgatgatcat ttagaggttt attttgaaca actggcaatt61 ccaggaatga tggaataaag catacgaagt agaaggactg gaacctccag aaaaagtact121 ttaagttacc tacaggtgat cctagtcagg tatgaattga taagaaatgc ctgcaccttc181 cctccttcct atctttccct tgcctacaga aaattaaaag gcaaaacaat agacatctgc241 atattcttca ttcagatcaa ccagtggcta gcatttgcca ccttttgcag tttctttctc301 tttccataag tactttcttc tctgaatcat ttgaaagcaa atgaaaacag tagcctaaag361 tgtcagtttc aaccagaaaa taacagctct gatttctcat ggctcatact cgtctgaaac421 gactcaggta gaggctgagg aaggccgtgt tgtttgtcta cctgggacta cacctactga481 aagaagttct caagttctga ttgagttcta aaattttttg aagattggaa ttcttcatat541 gtaaaaagag aagagaaaga cattcttgat tttggtgact agagttgtag atgctggaag601 ctttgccact aacattgatg aaccagatgc atgtcaccag cccatcacgg gcaccatggg661 cccggcactg ccacgttttc caaggaacct gccggagctc ctgcggaagc tgctcctcag721 gcgatggagt cccttcgctg gtaggccctt cttcagtcac cacaggatgc ctgccattca781 tgaacaagaa ggagaggacg ggctttgaat aaaaaacagg atccaggaaa tatttgtgag841 gccatttgga cttcagtgtg aaatggtgtt aaaagatgaa gtcatttatt caagaagtaa901 acctctgcca cctggactgt gcgcagacat ttcattgatt ttgtttaata aacattttct961 ggcttaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa21030211665212DNA213Homo sapiens400301 cttttttttt tttttttttt ctttttttag gcatatgtag taatattaga aacatttaat61 ttgggaaact ttgattcttg aaagagaaaa caaaagcatg tgaataaact ttgaagtgtt121 cacctcagtt tgggaccaaa ctgcttggat ctttgtaaaa accggttttg tatgtcaagg181 aggagtttaa ggcctttccg accaccttgn gttccccttt tctgcgcacc attgtatcac
241 gtggagttgc tccttaccac acctcacgtg cccctgagcc cnatttcctg atttcttctg301 ggctggactt ccccgttctc caccagcagc tccagtatcc caaactttct agtcctgctg361 atcctcccag caacggggtg gaaactggag ggcagtgtct ggtctgtttt ctaagaaact421 tatgaattct attatcntta caaatatgng aaaatttttt caanaatttt taataancct481 tttataaaat gaaaagaaac ccnatgatcg attaaggaag nggggtatgg cngggggggt541 cagggggttt ttnggggtcc cttttttttt cctggccttt aaccctaagc ggntaagtgg601 aagcatccca naagttnggg gggaaaancc cctaaaangg gccntgggct gcctctgggg661 ngggt21031211462212DNA213Homo sapiens400311 tttttaagat gaagacagta atataatatt ttggnacaaa aacagctgca ggangaggtg61 gaggggggcc tgtcattatg ttnccccccc accccccaac gaaaggaaaa ctaagactcc121 caacataaac agggccttga ggggggggat tacaggcact tgggcatgga gtcttcggct181 gcaggaagca ctccgcttat tcttcaggaa tgggaaaggc gtgacccaac gagagcatct241 gtctcagagc tccactcagg gtcacccctc tccagaggcc ggtatggggt ggcttcagac301 ttccactgca cgacctgggn gcaccaagac cacacaccac aataccaaat ttcacccaag361 aagaggtttc agcattgtgt aggttggagt aaaacttgca gagattncca ggggggtntt421 ccatggattt tctggggttt cagaacagta attntagtnt tt21032211440212DNA213Homo sapiens400321 ttgaaacacg ttccaaatac attataataa attgtgtgtt ttgctatttt tgggtgcagt61 ttttatatca aagtatttaa tcctactgct ttaaattttt ctattattat tgatattttg121 tatccttttt gctatcagtt attgaaaaag gtatattaaa gtctttatga ttgaagacgc181 ggctattgct ccttttagtt ctataaaaat tgtaaatgtg tgttaactgt gtgtgagtga241 atatacatac acaagtttga caacttcctc aatagttgat tcttttatat gggatgatgg301 gatacatctt cctttcaagt aataatttct gccttaaaaa tctcttttgt ctggatatta361 acatagctaa acaaattttc atttgggctt gtgtttggat taccctttca tatggcatat421 atggangggg nttttnaaat
21033211481212DNA213Homo sapiens400331 tttttttttt tttttttggg cttcaagttc atttattgat gcattggttg gacacaataa61 aaccacaatt gttatcaaaa agtttccctc ccctccccct ttttctggtt ctcccattgg121 aaatgtcttt aaaaaacaaa aaaaagatac agggggatac tggcagggtc ccatattgtg181 gggtggggag ggggcagtgc tgcagtggaa aggagtttgt gacattgaca ttgacagcat241 gaaaatgggt cttgccagtg catgtgagac tgcctcccaa cactcagctt cacagtggnc301 aggggctggt tgggagaagg gaaagctgaa tttagtgcta gggaccagtt tgggagtggg361 gaagctntag ggngaaagct cttcctttgg ggagctcttc ggggggtttc cggtttttna421 gctgagtncc ataattctga gggnagaccc acacccgccn tccaagattt tgggtttcaa481 g21034211454212DNA213Homo sapiens400341 tttttttttt tttttcattt gttattttct tttatttgtg aatatgtgtg gggttgggag61 actgggtgct agacatgagc tgaggtccag gctaggcaca gggagctgac agctgcagga121 catgctaatt ggtctgggaa ggcctttgga atctagggaa ttggctttct gagctgaggc181 gggtccaggc aaggaggcaa caggctgcag gaatcacaaa gggaccgggg acctccatgt241 acccaatatc taggtctcat cagtcggaga aacattctca gctcttgggt gaccttgtcc301 tccctcaaca cggtacttca ctgcatatgg gacttcattt gacaattgcc acaaaggctt361 tgggcttcag aggcactttt aagggatatt aacagtaggg aaaggggagg cttgggggga421 gnccagagag ttcacccaca caaagcccca cact21035211307212DNA213Homo sapiens400351 aggacttcat ctnatattca gattatagca agaaaatcaa tgtgctccat atatttnacc61 catgatgaaa cttgtcccat atccattact atatatatat atatagttta actgggtggg121 gttgggaggg agaaggcatg aattcaaaat tttaattaaa tattacataa tttaatgttc
181 tcccaagaat ttgagctgac attttcatat gaatacaatt tcacaaaggt tacaaatata241 tatcanctta caacagaagg natcatttag tattttacag tgtttttaaa aatctagctc301 cataatc21036211791212DNA213Homo sapiens400361 tttttttttt ttttttttga gacataatct tgctctgtcg cccaggctgg agcgcagtgg61 catgatctca gcttactgca acctccgtct cctgggttta agcaattatc ttgcctcagc121 ctcccaaggt gctaggacta caggcgtgtg ccaccacacc tggctaattt ttgtgttttc181 agcagagaca gggttttgcc atgttggcca ggttggtctt gaattcctga tctcatgtga241 tctgcctgcc ccagcctccc aaagtgttgg gattacaggt gtgagccacc atgcctggcc301 ttattttgtt gattctaaca cgtggtgata tctcctggtt taaagaagcg tttccctaat361 ggtatcaaac atcttttcat gtgcttattt gcaatctgta tatcttctcc agtgaaatgt421 tgctttgtat cttttgctca tgttctaatt gcattctttg ttttgttact attttaagac481 ttttaaatgt attctagata ctagtccttt gcaaatatag tcatgcatag cataaggaca541 ttttggtcaa tgacaaactg catataggat gcggtcccat aagattataa taatgggcct601 gaaaaaattc ctgttgccga gtgcaatgct actcatgtgt ctagtgatgc tcgtgntaag661 ctactgtgct gccacttgca ntaaagcaca gcgcacacca ttctgtacag tacacaatac721 ttgataatga tataaatgtg ttcctggata gcacagacac acattctgta catacacata781 cgtgatatga t21037211502212DNA213Homo sapiens400371 tccaaactcc cttatattcc tatctggaat gcgaaagctg gaaatacaag tgctctgtaa61 tcacaggcag tggttttctt ggagtctggt gtgatttctg tagcttgcag caatatgtgg121 gacacacttg cactgaagaa tttaatgact tgttttactg ctgtctgcca tcaatattta181 tctatattaa ctgaagaaag gcattactcg aaatggcatt tcacacctct gagcattata241 cagtaattct aaagtcatac gttgtaccaa attctctggg caaacacaga gatacagtat301 gaatggaaag aatccaagtt gaagttttta aaacttgaat tgtctgcagt aatctggaaa361 accaaagtca aaatgactcg aattgctatg gctgggaatg actggattaa aagcaatgga421 aacaatcgtt tctcggcttg gtttttcttg aattagcaag agcacatttc aagtatattt481 aatagaaaag aaaaactggg cc
21038211516212DNA213Homo sapiens400381 tttttttttt ttagtatttc ttgttatatt tcataaacag tctaacaaaa cactaacaaa61 gttattttgc ttattattct gatgctggaa taatttggtc aaatattcat aaatagttat121 caactttacc aaggcacaaa aatcaagaat acatgctttc tataaactca tttactactt181 taaagttttt aactgtttan cagttatagg aaattttttt aatcgaaaag aaaactattt241 acatttcaaa tacaaaagaa aattaaaatt attcttgatt gatctctaaa acccaaccac301 ttagatgctc attattatta ttttctttca ccaatccagt atactctatt caacatgtgc361 atcctgtgta cttactaagc atttatttgc acaaggattt tctgtgtcag gttaactttg421 ctgtgtagat tctgtgttag ctacctgaat tcgtccaana aaaagtctca cntttttttg481 ganaaccaat gctgctacnc tgtttccaga tctgcc21039211684212DNA213Homo sapiens400391 tttttttttt ttttttttca aaaagaaaga agtaaaatta tatttggttt gtagacgaca61 tgatcttctg tgcagaaaat cacaaagagt caaccaaaat actactggaa ctaagtaaca121 tattcagtgg atttgcacaa tacagtatca gcaccaaaaa ttagttgaat ttccatactt181 ttaacaataa acaactttaa aagaaaattt taaaacactt ccattggctg gagaacttaa241 agtaagaaat acttagaaat aaacgtaaaa aggtcagagg tttttacctt gaaatctaca301 aacattgatc aaaatgatta gaaacataga gctacaaccc atacttatgg tttggaaaaa361 ttaatattgt taaatgatta tataacccaa tgtgatttag attcaacacg gtacccataa421 aaatccttat cactggggct gggcgcattg gctcacgcct gtaatcccag cactttggga481 ggccgaggtg ggtggatcat ctgaggctgg gagttcaaga ccagcctaac caacatggag541 aaaccccatc tctactaaaa atacaaaatt atctgggcgt ggtggctcat gcctgtaatc601 ctagctactc tggaggctga ggcaggagaa ttgcttgaac ctgggaggcg gaggttgtac661 tgagctggga tcgctcattg cagt21040211738212DNA213Homo sapiens
400401 tttttttttt ttttttttga agtcagggca acttttattt acttgaacaa cagacatgac61 acacagcacg atctctcctc tccatcccat cccaagcttc tgtccacgac tacccagggt121 ggggaaaaag gcaatggccc agccccgatg acctggggga cttcagtgtg cctcctcagt181 cccacccaga aaaaagcctc ctcccatctg atttcaagat agtggagaaa agccaggcac241 ggtggctcac acctgagcca cctgtaattc cggcgcattg ggagactaag gtgggaggat301 ggcttgtggc caggagttcg agaacagcct gggcaacata gtgagacctg cccccccatc361 tctacaaaaa ttaaaattaa aaaaattagc caggtgtggt ggcgcatacc tgtagtccca421 gctctactgg ggaggctaag gcaggaggat cacttgtgcc caggaattca aggttgcaat481 gagccatgat cacaccagcc tggccaagat ggtgaaaccc cgtctctact aaaaatacaa541 agattagccg ggcatggtgg tgggtgcctg taagcccagc tactcaggag gctgaggcag601 gagaatcgct tgaacccagg aggcagaggt tgcagtgagc caagatcaca ccactgcact661 tcagcctggg caacagagtg agactccgtc tcanaaacan acaataaaag gccaggcacg721 gtggctcaca gctgtaat21041211551212DNA213Homo sapiens400411 gtgtacaaac aaaaatcact ttattcacat gccagaacag tgtttgagta gatagtttta61 agggcaatac aatcaatttc ttgaactcag atttgaattt ttaaaaaatt atattcaagc121 tctaagtatt ttacttacag aattttgcac atggctcact cctttctgca caaaggtaaa181 ataatatctt tatttgctta attccccatt gttttgttgt ttcaacatga ttttgtaccc241 tcagtctttc aacgttagtc aaacgttaca ttttctttca ctcttaccct tttttgagct301 tcatttcctc tctttatgtt ctcgagtata gcttcgaggt gtagggtatt cattcccaag361 atccacaaca attacctccc tagaaaatgt cactcctttt aaaggtcttt tttggctttc421 aacaactgaa ggagttctag tgacagatgt gtgtattttc tgcaaaccat catcctcttc481 ttgaccagtt tctccagcat tctcaggctc ttgcttctca tttatttggn tctcctcttc541 tgcctcccat g21042211747212DNA213Homo sapiens400421 tttttttttt ttttttttga gacagagtct cactctgttg cctaggctga gtgagaataa61 ggttgtgtca taaataaata aataaagtca tttacttaac atttacatag cactgattat
121 ctgacagaga ctgaggaagc agaaacgagt aagacaccac tccaggcccc agggatcttt181 cttgagggag attcacatcc tcgggaaaac aagatgtata gtgaatgaag gaggtagcag241 aggggtctgc agtaactgag taaggtatgg agctagagga ctaatacctc caggataaat301 gaatttatac aaagattgta tttgagagaa gaggtgagga accactcact agtagttttg361 agtgccctga aagtgaaata gattgaattt tgtcctggag gtcaatggga tgttccagga421 cttaagcccc ataggactga caatcaaaaa caggtctggc tgcactgtgg gaaatggatt481 gagggggcgc tggagtgaag gcaggaaggc tgtgagacgt gtcagacagg acaggttgaa541 cctggggctg agagaactga accaactgga aaggtctctt aaggtctctt acaaggtaaa601 aagagaccta aggcaggtgt gaagagcgat aaggcaagga tgagatccag gtttctggcg661 tgggtgtcac ggaaatgaga acacgatggg ggcagagctg gggaaaagct ggggagatca721 atttctcgcc gagcctgtgg tgtctga21043211699212DNA213Homo sapiens400431 tttttttttt ttttttttca ataaaagcat ttacatttat ttgcacacta aacacattga61 tttactactt attcccagtt ttcttattta cttatatagg atccattcat tcattttaca121 aatacagatt ttcagagtaa gaagagagaa taattcttct aaaaataaag gcattacaat181 agaatttgca aatctaggta ccacgtggaa agtctcatcc gttcccatat cattaactgc241 tcattttcac actagtgact tccaagtcct tattgtcagc atcctctatc ttcccatctc301 cactggagca tttccaacct cctgccacca actcaacaca ttcagggaga agagggctgg361 tctgggacca cagaaatcct tgttttaatc ttggaggcag tgctcagtat gaacttaatg421 aattgttaag tgaatttgtt ttttaagaaa gtcaacaagt aatgtatccc caggcaattt481 tatctgattt ttgacattta gattggtttt ctattacttg gcaaagagaa tggcgtacag541 tcactcctct gggggttctg catgaggaag tatccattat acatgccgtt aaggagtgat601 caggtaggga gggaaaggtt ggccacatca actctccttt gtgaagccaa cagctctcaa661 caagggagat atagtgctct gtagagcaga aacatgaat21044211728212DNA213Homo sapiens400441 tttttttttt ttttttttgc tgggggttag gattttaata taaagttttt tggtgggggt61 gggggagaca caatttgatc cgtgctgagt ccattttgtg ttgctctgac agaagacctg121 aggctgggtc tttataaagg aagaggttga tttggctcag attctgatgg ctggaaggtt
181 caagactggg caggtgcact cactgaggac ctccctcatc cctgaggctg ctgctgcttc241 tgggggaggg cagaaggatg cagctgtgtg cacagagatc acatggtgct gaaggaagca301 agggagagaa aaccaaattc ccaaattccc aagggtgagc actcactcac cccttaaggc361 aggcattaat ctatccatga ggactccacc tccaagacca cacagctcct atgaggcccc421 acatcccaac acctgccaca ctggggctcg aatctcaaca tgaattttag tgaggacaaa481 cgatatccaa accattgcca cccaaaagaa gtgggctgag gcctggcgcg gtggctcaca541 cctgtaatcc cagcaacttt gggaggctga gacagatgga tcgcctgagg tcaggagttc601 gagaccagct tggccaatat ggtgaaaccc cgtctctact aaaaatacaa aaattagctg661 ggtacggtgg ctcacatctg taatcccagc tactccggga agctgaggca ggggaatcgc721 ttgcacct21045211661212DNA213Homo sapiens400451 tttttttttt ttttttttgc agttagaaca tagtttattc tttaagtgta ggagtgcatg61 acataacact tgcctggcat gaccttagat cttacgaata atttgttatt gccacaaagg121 gtctattctg tcagccttat gatctttatt ttgacattaa tgctggtcag ttgttgtgtc181 taaaccataa aagagacgga gatataatga agcgtgtctg acctcccatc ccgtcatggc241 caggaactca gttttaaggt ttttctgggg tcactttggt caagaggggt tccattcagt301 caattagggg gcttaggatt ttagtttaca acttacttca ccatctctag ggtgttgtct361 ttatttatgt ggtccagaat gtctacatgt ggccaactaa aaattgatga ctataagaaa421 gggaaagttg ctttaaggac gaccccagta tctggcacac tctggcaact gaagtccaga481 gctgaagcat aaaaccaaag gaaacaaaaa gcagtctcag agcatatgag aaatcaaaga541 gtttcttata tactgcagat agcaatttta aaaatcagta tttttaagct cagagcattg601 gatgccaaca acccatagtt cctgcacaaa gacatggaaa aatccagcca caggatctgt661 c21046211677212DNA213Homo sapiens400461 tttttttttt ttttttttac gtgttgtaag attccattta tatgaagtgt ccagggcagg61 aaaatccaca gagacagaaa gtagattaat gggtgtgggg ctgaggagag gaaggcattg121 ggagaaacgg actttttctt ttagggtgat ggaaatgtgc tgtaattagc ggtaatggtt181 ccactctatt gtgaacacgc taaagctact gagttgtaca ctttaaaatg gttaatttta
241 tgttatgtaa attttctctc aaaaatttta aacaaaatgc acagggaacg agcgtgggcc301 ggtgatgccg gcaaccgggt gcctgttgct gcgctgctgt ggcgccccct gctggctgcc361 cgtgaattcg gggagggatg gggcctggat gcgccggtgt ctcctgcttt ctgttccgga421 gacttcccag gagggtggag accccaccca gagtgacttc ccaaaaggca gcactacccg481 acgagggacg ccacaggagg aggacttgga gggagggcag tgggcacggt agagtcttct541 ttcctgaggc gaccccagct tgttggacga ggcacagcan atgctgacta tagtaataga601 agagtgagtt atggcttcag ggattttttt aattattact tttacttcca gtttntctat661 agtgagtaca gattact21047211635212DNA213Homo sapiens400471 ggcccagatt caacttttct atgaaaaata gaaactgtca tgttgagtca taaaataaaa61 aaactagcaa atccagctct atgctcagag aattaccaga aaataaaatt acatgaagct121 tgaatatagg gagatggaaa gatattagac aaatattaaa gaaaatctgg gccaggtgtg181 gtggctcaca cctgcaatcc cagcactttg ggaggcccaa ggtgggaaga ttgcttgagg241 caaggggttt gagaccagcc cgggcaacat ggtgaaactc tgtctcttta aaaaagaaag301 aaaagaaaag aaagaaagaa aagaaaatct cagtgagtga tggtcagaat agaattcaac361 ataacaagct cattattaaa atatttgatc tcactgtgta caattctgaa gacactcatt421 catgtacttc attaaatatt tctagtttgc taaaaataga attacccttc aacccagcaa481 tcccattact gggtatctac caaaaggaaa aaaaaaatca ttctatgaaa agatgcctgc541 acttgtatgt tcatcacaga actatttaca gtagcaaaga cgtggaatca acccaggtgc601 ccatcaacag tggactggat aaagaaagtg tggtc21048211678212DNA213Homo sapiens400481 tggtagtgga cccggcctcg gaccgcgtgc tggccaccgg ccacgactgc agctgcgcgg61 acaaccccct cctgcacgcc gtcatggtgt gcgtggacct cgtggcgcgc ggcagggccg121 cggcacctac gacttcagac ccttccccgc ctgctccttc gccccggccg ctgcccccca181 ggccgtccgc gcaggcgccg tgcgtaaact ggacgcagac gaggacggcc tcccctacct241 gtgcactggc tacgacctgt acgtgacccg cgagccctgc gccatgtgcg ccatggccct301 ggtgcacgca cgcatcctgc gcgtcttcta cggtgcgccc tcgcccgacg gcgccctggg361 cacccgcttc cgcatccacg cacggcccga cctcaaccac cgcttccagg tgttccgcgg
421 ggtgctggag gagcagtgcc gctggctgga cccccgacac gtaggcgccg ccctcctgcc481 tccggaccct tcccgctccc ggccgtgggg cgcccctcct ggacttccgg gcctcggatt541 tcttccgcac aagcctgacc gtggatttca gggacacata ccgctccagc gggggagcac601 gggtgctgcc ttccgtgcgg atcgagcttt cctggactcg gtcattgggg ccaccccgtg661 ccaagcggtt gcccttct21049211837212DNA213Homo sapiens400491 tgcatttgaa agctgaagga attactttag atagatagga attgctacct ttattctgga61 aaggtttagt ttctgcctca aagcattttg aagtgtttta accattaaag ggtctaattt121 ttttttctta atgaaatcaa gcattttaat ttactgtggg aggcatcctg accacggaca181 tccataacag caaagcacaa atcgttttcg tctgtagtca tatcctgaaa cataggtgga241 caaattttta actgagagac aaaaatcaca tagttgaatt gagcagaaca cttaagtgct301 ttctgcatct atttaggagt ctatttctta ccaataaact tgacaacgca tttggaaaac361 tagtgaacac cttacagctt tcattctgct ttaatgtttc aattcaagcc ggtgtaaaaa421 taatttccaa ggcatttctg tttattcttt agtaatctca ctactggcta tgtcagcaat481 atctttttca atctggttcc ttttgtatat gatgtcactg tgacctcttt gaaatatagt541 gatggctttt acctactttg aaaagaattt tcacatagag tcagaaaaaa aaggaactat601 caaatcactt gcctttccac ttggagagca cgacagttgc caacaacaag gggtcaaggg661 acgcacagga gatgtgtggg ggcctggaca ctcccaactg gtgaccagag gcttgttcgc721 atttacattt gtctccactt gtaaaaaaca tagttcaggt cctaaattct tatcactgga781 tttacttagc aatcttcaat gacctgcagt cattgcctgt ataagtgaac acgaaaa21050211819212DNA213Homo sapiens400501 ctgcctgtct cggctcccaa agaactggga ttacaggcat gaggcaccgt gcctgtccca61 tgtgtactta atagatattt accatataca attatttttt aatttaaata aatcaatgaa121 cagtgttatt ttttaaatct tgtggcttta atacaccctt tcaattctca gggagtgttt181 tggtgataca ggctggggag ttaaataaat gaacgaataa acttagttgt tatccattca241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacaaa aaaaaaaaaa301 aaaaaaaaaa aaaaaaacaa aaaaaaagaa gagagagaga gaagagaaga aacaaataac361 aaatcaaaaa agaagaagca acaaaaaagc aaagcgtaaa caacacagag agcgcgggcg
421 cgagcagagg cgagcccccc ttgagtgaga acacattata aaataccgca gcgaagacga481 gggtagcgtt tacagaaaga cggagcagcg ttcctcgagc gggagggaca acttcaccta541 ttaacaacct gaatctagct tgtggaagaa cagaacaaca ccactcggtc ggagggagga601 cacaaacaaa tgacgtgata acagtaacat ctccatcctc tgcatcgaaa ggattaccac661 acccccgttc gggcggcgag acaaaaataa atgatagtga aagttacata ctacagcggt721 agcacaataa acgacacgtc agactactat gatcggctat aatagtcata aatgtttgcg781 agcacaagcc gccgagttta ttcgatcacg agacctctt
權利要求
1.一類人類肝臟表達序列標籤的序列，其包括(a)SEQ ID No.1～SEQ ID No.50所示的序列；(b)SEQ ID No.1～SEQ ID No.50所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.50所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數條的組合。
2.根據權利要求1所述的一類人類肝臟表達序列標籤的序列，其特徵在於所述序列包括具有SEQ ID No.1～SEQ ID No.50所示的序列。
3.一種探針分子，其特徵在於所述的探針分子含有權利要求1中所述的序列中約8-100個連續的核苷酸。
全文摘要
本發明公開了一類人類肝臟表達序列標籤。利用本發明的在人類肝臟中表達的表達序列標籤，可以方便的尋找出在人類肝臟中表達的相關基因，從而在研究肝臟疾病的致病機理以及開發治療肝臟疾病的藥物中發揮重要作用。
文檔編號C07H21/00GK1955289SQ20051003082
公開日2007年5月2日 申請日期2005年10月28日 優先權日2005年10月28日
發明者黃健, 韓澤廣 申請人:上海人類基因組研究中心