人類肝臟中表達的表達序列標籤c組的製作方法

2023-07-27 19:07:46

專利名稱：人類肝臟中表達的表達序列標籤c組的製作方法
技術領域：
本發明涉及生物技術領域，尤其涉及一類在人類肝臟中表達的表達序列標籤。
背景技術：
肝臟是人體內最大的消化腺。也是體內新陳代謝的中心站。據估計，在肝臟中發生的化學反應有500種以上，實驗證明，動物在完全摘除肝臟後即使給予相應的治療，最多也只能生存50多個小時。這說明肝臟是維持生命活動的一個必不可少的重要器官。肝臟的血流量極為豐富，約佔心輸出量的1/4。每分鐘進入肝臟的血流量為1000-1200ml。肝臟的主要功能是進行糖的分解、貯存糖原；參與蛋白質、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防禦機能；製造凝血因子；調節血容量及水電解質平衡；產生熱量等。在胚胎時期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等。現代醫學實驗證明，肝病病毒侵入人體後，並不直接引起肝細胞的損害，只是在肝細胞內吸收營養賴以生存，並在肝細胞內複製、繁殖。其複製病毒的「零部件」如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細胞膜上，引起人體免疫系統對這些抗原物質產生免疫反應，這種反應造成肝細胞的損傷、壞死。免疫反應的強弱決定於肝臟受損程度及臨床症狀輕重。這場由病毒引發的、免疫系統對肝細胞的戰爭，使大約25％的患者的肝臟成為戰火連綿的戰場，肝臟的損傷由此加重。肝病的危害絕不僅僅限於肝臟本身，它還可以引起其它多種疾病。常見的有(1)糖尿病；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎病；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結節性動脈炎；(12)消化性潰瘍；(13)自發性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對患者的身體甚至生命造成危害，而且對患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學等方面都會受到嚴重影響。
生物基因組中可轉錄表達的序列(即基因)僅佔總序列的3-5％，對這部分序列進行測定，將直接導致新基因的發現，並獲取基因組中與產業化關係最為密切的信息。20世紀80年代，高通量的自動測序的出現，使從質粒互補脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫隨機選取許多cDNA克隆和決定來自非載體兩端的幾百個鹼基的DNA序列成為可能。這些短的DNA序列叫做「表達序列標籤」(Expressed Sequence Tags，簡稱ESTs)。表達序列標籤的概念最早是由Adams等在1992年提出來的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics，2，173-179)針對獲得大量信使核糖核酸(mRNA)序列的迫切需要，提出大規模互補脫氧核糖核酸(cDNA)測序的研究戰略。隨後Venter創立了大規模表達序列標籤技術。其基本特徵就是從以質粒為載體，構建完成的目的組織互補脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫中，隨機選擇許多cDNA克隆，利用質粒上攜帶的通用引物對cDNA兩端進行一輪脫氧核糖核酸序列測定，所獲得的來自3』端或5』端的幾百個鹼基的非載體短脫氧核糖核酸(DNA)序列。簡而言之，表達序列標籤是來自表達基因片段3』端或5』端的短脫氧核糖核酸序列，代表一個表達基因的部分轉錄片段。
表達序列標籤可用於新基因克隆、人類基因組圖譜繪製、基因組序列編碼區的確定等。如果一個表達序列標籤在基因組中只出現一次，那麼它可以作為序列標籤位點(STS)。由表達序列標籤構建的物理圖譜叫表達圖或轉錄圖(expression ortranscript map)。利用表達序列標籤進行基因圖製作，可以加快序列標籤位點的製作和新基因的染色體定位。表達序列標籤可以作為基因特異性探針，對組織特異性基因表達的研究具有重要的作用。表達序列標籤還可以進行新基因的遺傳進化關係分析。表達序列標籤可以對所有動植物的基因作為一種資料庫，通過不同的序列比較可以獲得保守序列片段，從而獲得基因的遺傳進化圖譜。正因為表達序列標籤具有如此的優越性，因此表達序列標籤測序已經成為許多基因組研究機構的工作重點。
表達序列標籤(EST)是一種快速有效揭示基因組容量的方法。ESTs在新基因的發現、基因作圖和基因組序列編碼區域的確定等方面起到重要作用。EST不僅為基因組遺傳圖譜的構建提供了大量的分子標記，而且來自不同組織和器官的EST也為基因的功能研究提供了有價值的信息。此外，EST計劃還為基因的鑑定提供了候選基因(candidantes)。
在本發明之前，還沒有出現涉及本發明的一類在人類肝臟中表達的表達序列標籤的公開報導。

發明內容
本發明要解決的技術問題是提供一類在人類肝臟中表達的表達序列標籤。
為了解決上述技術問題，本發明通過如下技術方案實現在本發明的一個方面，提供了一類在人類肝臟中表達的表達序列標籤的序列，其包括(a)SEQ ID No.1～SEQ ID No.63所示的序列；(b)SEQ ID No.1～SEQ ID No.63所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.63所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.63所示的序列。
本發明還提供了一種探針分子，所述的探針分子含有上述序列中約8-100個連續的核苷酸。
由本發明的一類在人類肝臟中表達的表達序列標籤，可以方便的尋找出在人類肝臟中特異表達的相關基因，從而在研究肝臟疾病的致病機理以及開發治療肝臟疾病的藥物中發揮重要作用。
具體實施例方式
下面結合具體實施例，進一步闡述本發明。應理解，這些實施例僅用於說明本發明而不是限制本發明的範圍。下列實施例中未註明具體條件的實驗方法，通常按照常規條件如Sambrook等人，分子克隆實驗室手冊(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照製造廠商所建議的條件。
實施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源於5個成年男性，在肝臟切除手術後，將肝臟組織立即置於液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩後，再移入玻璃勻漿器內，勻漿後移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑑定總RNA質量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實施例2cDNA文庫的構建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補平末端後，加含EcoRI切點的接頭。磷酸化EcoRI末端後，用XhoI限制性內切酶消化1.5小時，再進行片斷分離。過柱篩選長度＞500bp的片段，用酚-氯仿抽提，乙醇沉澱，無菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進行包裝，宿主菌使用XL 1Blue MRF』(Strategene，CA9203，USA)細菌。塗板並測定滴度。
實施例3測序及資料庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆，擴增後抽提質粒(Qiagen Germany)，用T3和T7作為3』和5』端的通用引物，採用終止物螢光標記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測序儀(Perkin-Elmer，USA)上進行EST大規模測序。測序結果用FACTURA軟體去除載體序列，傳輸到SUN Ultra 450Server上進行下一步的處理。所有的序列信息再用GCG軟體包(Wisconsin group，USA)中的BLAST和FASTA軟體搜索已有的資料庫(Genebank+EMBL)，將無同源性或同源性低於95％的序列視為新基因建立資料庫。
實施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎上，進行cDNA全長克隆，分兩階段進行(1)「電子克隆」(Electronic Cloning)以新基因片段序列作為探針搜尋dbEST資料庫，將重疊序列＞50bp，同源性在98％以上的表達序列標籤(Expressed Sequence Tag，簡稱「EST」)序列認為同一序列(Consensus Sequence)，取出並用AUTOASSEMBLER軟體進行連接，部分EST可以延伸探針序列。再用STRIDER軟體分析被延伸的序列是否具有完整的開放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和胺基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法，通常可獲取人肝臟相關基因的全長序列。
(2)cDNA術端快速擴增(Rapid Amplification of cDNA Ends，RACE)如果通過「電子克隆」方法仍未得到完整的cDNA全長，則在已有序列5』或3』端設計引物，在人類肝臟Marathon-Ready cDNA文庫(Clontech Lab，Inc，USA)中進行長距離PCR反應。然後對PCR產物克隆、測序。用AUTOASSEMBLER及STRIDER軟體分析被延長的序列有無完整的ORF，如無，重複上述過程直至獲得全長。
(3)RT-PCR對於5』和3』端的已知的序列，如果中間有一段間隙(gap)無法從已有的公共資料庫或自身資料庫獲得，可考慮採用RT-PCR的方法。在序列5』端設計引物，3』端引物採用Oligo-dT，在肝臟總RNA庫中進行擴增。然後對產物進行克隆、測序。最後拼接便獲得全長。
通過組合使用上述3種方法，可獲得人肝臟相關蛋白的全長編碼序列。
序列表110上海人類基因組研究中心120人類肝臟中表達的表達序列標籤C組130NP-10046160632101211574212DNA213Homo sapiens40011 gtgggagata ggggaatata ctatcttcta gtcctttagt tccttttggg aatgtcataa61 ttagtttgtg cagacttctt gagggtatgg tatattagta tgatagagct atttggatat121 cagcccaaag gattggtagt ttagcaatac agggggaaga aaaaagaatc tttggtagca181 tccttcggac cactgaatca accttccctg ggacttctct acctctgggc tttgggttat241 atttggacca tctggagtta gttcttctgt tttaagtcta aagcatccta aatgagatgt301 ttcttatgct tctaaatata ctctttttaa ccatgttcca ttttgtgtgg acattgtaag361 ggtaatatat acagacttaa aaagaatatg cctatgaatt aggaagcttt aagcaatgga421 aaccatnttt cattcactgc cantccacta tgggtagcaa ttcttccnat ggnaattctg481 ttngttactt ccncagtaat aaggaagaat catcagtnca attggtgtaa ggtactttta541 atggaaacat atctatattc nccaacngta gggg2102211476212DNA213Homo sapiens40021 ttttaaacat taatttcaaa ctatttttat ttaacatttg gtattgttaa acaggtatag61 tattcaggac atacaccttc taccacaatt tctttaatta gggtacttta tattttatca121 aaaatgtgca tattaataga ttgtagttta gcctttgtca aaagacatgc aagtatttag181 tatttacatt aaaaggattg cctcagaaga aacattgaat tcataagccc tcttaaaaag241 tcaaaaatat gcataaagaa gactagtatt gatattttct gatattttag atatgattaa301 tattttggac ctccattggt ttccacacta tcatgcaaat ttccaacttg ctttaacaca361 acagttctat cagctgtatt agagggaata cacattttaa ttgctctttt tgaaaatatc421 accaggcagt tcagaatgtc tcatttattg agaatattgt gacaatcaaa gacctg2103
211486212DNA213Homo sapiens40031 tcattgctaa ggaagaactc cctctgctct tggaagattt tctactctac tgatcttatt61 ttattttatt ttatttttac ctgatgattg tcttaggcac cctccttaca taataaacca121 tcaagctaac ttgaacaggg aaactgagtc acactcaaac aatagctaag gtcaaaagtg181 tagtgaaagt agaaaaagtg gggaagggat aggtctaagt gagtgacaga tgggctgatt241 cagacagggc aataagcaca gggagatatg aagacaacta ccaaagcaag tggaagacaa301 ggttttcaac tttattgtat tgaaaaatac ttgtcacttg gttcagatgg caaatctaaa361 atgagcccac aatgattatg taataaatgc agaacgtacc acaacaaatc gagactaaca421 cagaaacaga agatgtgact ttgtgaatac aacgccgctc agcgcttcac tgctgatgta481 ggagtc2104211455212DNA213Homo sapiens40041 tgaccaaaga agcaggagaa acatccaatc tatatctttt cttcatgttc caattcagtc61 attgtttctt gaatgtttct cccaggaaga attaatttca ccaatgaaag cactggaggg121 aatggtgcct gaatatttaa aaaaatgaaa atctctctca tatgtggctt ttagtaacag181 gggaaacaaa gtaaaatgca cctcaagcaa gtgtgagang ggcgaggaag aaagcttttc241 agaaaggtgc attagcgctc acaacagcgc cctctgctgt ccaagtggtg gctgaagtga301 aggtggcttt catgctatca gaaaagccct tttcactaaa caaaattgat gttatctcct361 cttctaggct ctagagaggt cagaaaagga agagcaaaga attagcaata agtcagacag421 acagcagtga cctattggaa gaaaggaaag gttac2105211428212DNA213Homo sapiens40051 aaactgttat ggaaaaacat tttattacac atattcaact tgcttccaat gaaatgatta61 atttttctat ataattttca tgtataatgg cgtccaatgt ttgtcttccg ttttttttcc121 tttcaaaacc agccccaaaa agtaaaacaa aaattccatn gggtacagta tgtaatgcaa181 gttaacgaat gggnaataga tacatttgac tctgaaacga aaaaaggaca atcgtattgc241 catagaggct cttttcctgc attctgatcc tatccagcaa cggtcagctg agagggttta301 attttgcacc aataaaaata agcaccgtag ggtcgtgaga ctcctgggta aacacgtgcg361 ttagtggaca cgtgttgcaa agcacaaaag ggtacagtac tcngaggggg accaggtacn421 ggggttgt
2106211460212DNA213Homo sapiens40061 ttcaagattt taagattttc attttattaa aaatatagca acataatagc agcattgttt61 gataaatctt tactagtacc atttaaaagt agtcctactc acaaataacc accacctatg121 cagcatgttt atgtttacat tccaaactct tcccttgata atctaacact gttctattaa181 tctaatcatt aaggaggtaa ttttttaagc attaagttga tctacacagc tacacagtat241 actcaaattt catacttaaa tctattgtag aaatcagaaa ttctttagtt tattccccaa301 cagtgtgtta cctaccagta ccctcaaaat gggttgaaat caacctattc tttcagtgtg361 atttttatga atttatactt acatatggta gttaagaaat tatgtctagc anataanaat421 aaaccaagan tagaaggtac tactcctcca taccaatcct2107211411212DNA213Homo sapiens40071 ttttacatgg attctagaag tttattagtg acataaatat gaagttaatg gcatctttta61 taggcacaac aaaagaaaca aacgtattag aaggtatttg cataatcaga ccattttgag121 cctggtatgt tttccccagt aattggagca tccttagatg ccttaacaac aaggacagtt181 tctccatcct ttgctgcata catactttct aatactatct ctctccttcc actcccaatt241 cctaataaaa atctctgatt cacattaaaa acttattctg gcaagtctcc ctcttccacc301 tctactgctt tctatgttag tgacaaagtg ctattcatat aaaatnatac aaaaggcaag361 aaacatnaaa gttaaaagga aaccagtatg ggactgatgc cttcanacct c2108211476212DNA213Homo sapiens40081 tctggtagtg atttatatat atttattata gatttataat aaaataaaaa atgctattgg61 aattctatat ttacacatgc tccatttata tacacgcaaa caaatgtcaa tatcaatggt121 aaaattttag gattttaaag aggcacattt cctcctacct acccacttcc acatattcat181 tactctaaga tgagttttca taagcaacat ttagatttgt gaaggaaaaa aatgaatatt241 tctagtactt atcctctttc ctttcatgct tttgtttgag aaaggaagca gcacataaaa301 tctaaaacag aaactaaggc tgtaagacct tctgtaatac atgttcacac tcatgcatga361 gttgactgta ataggtcaga agatatttga gcaaagtaag cacttacatg tatatgcact421 gcntataagt gatactcctc agacacacac acacagacac acacactcat gtttcg
2109211610212DNA213Homo sapiens40091 agaaaattac attactttct ttctttgttt cacattacaa aatctttttt tctttacaca61 aatcacattt tattgcagga atatttcaag tgccatcaaa tatttataga agggttaaaa121 aaatagaagt ctctctaaag tggtccagac aaggctttgt atagaataaa tctttttttc181 cccatcttct agttttgatt taagtatttt gaatacattt tcttttccat tgacacttag241 tagcctaaga agcggtccga cgcacacaca tcatacacat gcaaatcaca cacacacaca301 cacacacaca cacacactcc ttcctcacct gaccctcagc ccacccccat acgctcacag361 ataactgggt atccacacta taaagaacaa ccaaacttag aagcagtgtc ttaagtcatg421 tttttcttaa gttagaaagg gtgaattagg atgctgaaga ctttaaaaaa aaatccatag481 ctttaataca cgttaagatg ctgaacactt taaaaaaatc cgtagcttta acacaattgc541 aactgtccaa aagcactttc atcggacttt ggttgcatgt gaccaggcca ggttttcnca601 tatataccat21010211467212DNA213Homo sapiens400101 ctgttgttct attttactaa atattagtta ttttccaaat aaattattgg caatgtatgt61 taacaaatat taagttgaca aagtaactta ttgaactatc accatatttt tattcttaat121 aaacttcatc aattacatgg tacatatatg ccattcaaga tcgacattct tttagaatac181 tacagagttg tttccacttt aaagcagaga aaattgaggc acaggatgat tcaatgattt241 gcccctgtcc tcctgccaat aaagggtcaa actggaatca gaagtcagct tttctgatgg301 aggctaactc ctgtaaaatc gcatgtggag aaaggactct tgtgtttgac agtctaaggg361 ccttttctgc ctaatctaag gtctgtttcc ctatatgcag gctgggaggg ggtgattgtg421 cagagggacc cttggagaac tgttacatta tgcaaacttg attatcc21011211503212DNA213Homo sapiens400111 ttttttttct aaaactacct ttattgtggt tggctcgaca taagatgccg ccatcagcag61 aattataaaa ctgtacagga ggcacaaaaa taggctgttt aacttagata atgaccctca121 tgtcttcaag ctttaaaaat gcacataaaa gttgtacaat ctggcagttt ataaaatata181 aagctaaaaa gaggattttg ggttccacaa agaagactgt atcacacaat taacacgtac241 taattaaaca attaaccatc cacacagaag acataatggc acagaattct taaaaatcac
301 ctaaaaatta acattttacc ccgaccaaat taatcaccct ttaataataa gactactgta361 aagcatccat cccatggaag gntacagtat cacttttcca gttttaaaat gggcaagggg421 tgttggtccn aaacaaatct gatgtggcct taagccagta ccattgtaac tggggtacat481 ttccgngtaa aaatttaatn att21012211558212DNA213Homo sapiens400121 aagagttttt ttttttttaa ttattccttc atattcaaac ttcacaaaca gtgtgaactt61 gtacaatacc tcggaaagtg aaacttacaa aaaaagtgct ggtaacattt aaaaaaaaaa121 acaacaaaaa ccccaaaaaa acaaacntca ttcttagcaa catcaattac tcttccacac181 aaaacagaaa ccttgtaaaa tttattttcg tatttttaag gcgtaatact tccgtataaa241 gtatatgcaa gagataaaac ttcacagtat tccaaaatgt cacaataata ataataatat301 aatagtataa tgaagcgcta cagttaattt ttcntttttt gaatgttttt ttttctgttt361 aaataacaaa tacaagtcac aggtaaatat acgtgagaaa aatacgaggc taatattaaa421 tggcngggta aggatactgg tcacngtaag aaaaaactgg cagggatgtg tgtaatttag481 nccatatttt aangcccctg gatagaaaag ggcggtatgc caagggtttt tccnaaccaa541 ttttnccggt tgggtggt21013211582212DNA213Homo sapiens400131 tttttttttt tttttttttt ttttgtttta tataagcttg tattcaaaat aaaccgatat61 tcattacact tacatatttg atagttacaa attataatgt acattataga tacttgcacc121 tacatatgca cataaactgg ctataacttc tttaataatg agattgcctc cgtagagtaa181 atgacacaat aaatgttcaa ctattttgaa tggttgctat ttccatttta tggtcagtaa241 tatgtcttca ttgttttgaa aatgtaggca tttgaaatta agtagtctac agcattcatc301 tgtagtaagt gctaagtatg ttatacatga acaaaaacac ttataaaagc atcttatgca361 ttcctttgaa acaggtgaaa aataacagac ataacttgct ttaaaaagtc ataaaatagc421 tgcttttcta ctgataacat ttttgctttt agcatttcag atgagtattt ttcatatgga481 gaatttataa atgtacagga aacatgttca tttaccaata tttatagacc acataatttt541 gtttgcataa taagtgtaat tgtagatncc attaccatgc ca21014211617212DNA213Homo sapiens
400141 tacatatttg gtttaccttt agccagttct gtccactctg tctggcaata atttttgctt61 tacattttta tctccagctc tacatgaact aactgaaagt aaaatggtcc agaatttctt121 gtttctgatc agtaattcac atattgatga tgtctgtgta tcctggtatg acttttctga181 tttttaatta taatttttaa ttttaattaa tttttgttta gatagtttat ccctttctaa241 gtaccttgct ttttaaaaag tgtaaacata ttttgcaaca gtcacaccac ttctgcctta301 ttagtaactg tgaaacaaag gacctgcact acgtaaatgc atattagtga atgtgagtaa361 aaggtacaac aatgtctact gggatgagca aataatgata aaccgtctag gcaaatattt421 atcaactttt tattctacat tcaatttaaa aaaataacat tttagtgacc aactaaggaa481 tagccaacan aaattgcacg caaaaagtaa catgaaataa gacattgtgc ataattttgt541 ggaaataatg ctaacgagna aaataggcat accccagctt accctatgnt ttcccttaag601 gtcctttggc ttnattt21015211660212DNA213Homo sapiens400151 taatttcttt ttctgaggaa caaaattggc catttaaata aagttctttt ctagtgagta61 aagaggtttc tcagagccag aaatcactga aaagagagtc tgtcgaccct gcccatggaa121 aagcccagag atttctctaa ccacttcttg ccaaacttcc tagttttgtg attggatgtt181 tcttggttta gcattgtttg gtgctgaata cattttgcaa ttcttttgtt ctctgcccta241 gagcttccat taccctggac ttcaactcat taccaaatta aagaattctt ttagaagatt301 tcttttaaga gtcaggtcac aacagaaagg gcgccctcct gttctcctaa atctcaaggg361 catctcaggt tgtgtctgct cttacgttgc accttccttt tgttcccttt taatgcagca421 gcacactggg ctatagcaga ggcagacctg atttgattat ctgatacctt ttccacaaaa481 atcatatatc ccctgcttcc aggcccnact aatccaaata acatcttcta gaacaacagg541 ggaaagctcc cagaaagggg tngaaaancc tgtgacttcc tggccagatc caccttgctn601 cctgtcttcc taggggtcna ggtgttggag natgtggagg agccccanca agggagnacc21016211544212DNA213Homo sapiens400161 atttcataaa gctactttta atatattaac atttattgct cttgagttca aagtatatct61 tattctagcc accaaaacat ttagaaaata gaaaaaaagg taaaaactaa tttttttatc121 tcttatattt cagacactta atacccttac cccattcact gaatccaatt tcattgctat181 ttccttgttt tgtccttttg agtggttcgg gatctgcttc aaatgacaaa gagatgtgag241 taatagggtt caacgtcttt cttattttca gatttaaagc tcttcaatga tgtagctcaa301 aatgaaagtt ctttcaatcc aacaactttg cagagatgct gatccctctc ccaggtgaat361 cctggccagg cctttctnac aaatcctggg gatancctgg ggccagtata gagttcaagg
421 gcagaccaga ctnggggagg ggttcctgan ggacctggaa ccagcatcca ccagcacagt481 cagaattgaa ccccatgtga atccttgtna aaggactggg ccctgaactg gttaganacc541 gtta21017211543212DNA213Homo sapiens400171 tttaacaatt tatagtcctt ttaatagttt tttttttttt ttcataatac tactgagggg61 aattgttaga tgtattatgt aaggcattct taatttagtt attaaagtta catttttaat121 atttttaaac cttttgtaaa tgctggctta attagaaaat gtttacagaa aagtaaaaaa181 attctagtaa tatgggaaat ccttgtaagc agcatggttt cagaaaaatc tcaagatgat241 ttatttcacc aaatgagtat tttttaaaac taggaactcc ccaaccaaaa acacagactt301 gaataatatt tgtgttatta cctttattgt acattgagca agcacccttg tatagaggaa361 atgcctcttt cctcatctat aatatctata gtatttaggg cctggatgta agagtgtttt421 tattttacna atagttaatg tattaaatat taagttagtt tccctgattt cccttatttg481 gttggcganc atacggggag naattggaaa ggcctatttc caaagaanct atggcactnt541 ttt21018211590212DNA213Homo sapiens400181 ttaatatttt aaaagcttat tgaatcacaa gcattttttt attttactgt aaaaacatca61 tctttatcag ggagggggga aagtacaaaa ttatgtccct gatatgattc aaccatgtaa121 aatgatgtac atttatgaac gacgactaga agtgaacatg aataactgaa aacaaacagt181 gtgatgcaag tgaatttttg gagggtgaga tggtcattat attgttcttc gagcaattaa241 atattttatt ttcttcccaa aacaatgtcc acaagggggc agacagaaga tgacaaataa301 aaccatttaa taaaaacctc agctgaaaag ctaataactc cagaatgcag gttgaaagca361 agcttaaagg tcatctaggc tggggtcagt agctcacgcc tgcaatccca acaccctggg421 aggcccaggt gagaggaccg ctcgagcccn ggaggtaaag gccgcagcga gctatgaccg481 cgccactgca caccagcctg tgccacaaag taganttcgt cccaaaaaaa aaaaatcctc541 ctagtcntct agtccatntc cccccctggn caagaaggac ngaggcccca21019211463212DNA213Homo sapiens40019
1 naggtttgaa cngactgtag tantntgtaa atgtgaattt tacaaagcgc tttacaatta61 atgatcacat ccttttgtnt gtcatggatt tccactgtct gaaacggctc tgagcacgct121 tgaagccctc ggtttccctg ttcgcttttg aatgtttcag ttttagttat tgatacaatg181 tcagccatgg ctaaaaagta acagtcttga ctctaccgag taacagcaca aaaacaggag241 tgagggctca ggaaaacaaa acaaaggctt cctccttaaa aaaaagacaa nnaaaaaaag301 ctaagcanct gtgggcttga aatctaactc agtnggtact gttgaaacct tccttttaca361 gcacaggaaa atttattttt taacagtcgt gagttacagt actttaaccc ctaaacagac421 tctttaaaac aaccgtctcc cttttttaaa aggtctcttt ttt21020211610212DNA213Homo sapiens400201 tttcccaaga ggcagggtgg ctttatttga cattagaaac ccatttaaaa cggcaggtaa61 gagcatgaac aaaagcaaag tttagttcct cgaatgcctg cttttgtaat gcaggtggat121 ctggctctag tctttaaggt tattaattgg aatgaatgcc ccaaggtgct cttagtttct181 ctgtgaagca ttttatatgg gagctctggg gtgactctgg gtccacagtc atgttcacaa241 gccatctctt ccgcagangt gccctgcctt gaaggctgtt ggctgggtcc ccagatattc301 tcagggcaag atctgttgtg ttataaccac ctgccatctg ttggtgcaaa ttttctggat361 tctagatgat gaatcacttt acttctcctg ctgttccttc tggctgccaa gactaattta421 cctattggac tttatgaggg aatatactag agtaacctat catgaaggtt tgtccccatg481 caaagttcct actccatcac tagaaaaggt gggcnagagc ttcccttagc atctccagag541 ggtgggatga agagccagca gtaacaatgt gggatcccca ncttggggtc cctttgcana601 aaaagccaan21021211543212DNA213Homo sapiens400211 tttttgggag ggggtgccca gcagacacgt tttaatttta ttgcggaagt gatggaagtg61 gttcatcact tacaggttaa ctacacattt gaggtgaatt ttagaccaca gaaacaggaa121 gtagtaaaca tctgtgatga aactttgata cttcaagatg cttgtcttag ggaccaacat181 tctctcagac tagcagatta tttccatggt gaatgagtct tcttattaat ggtgtgtgaa241 ctatatacat gatgactgaa agcagctcat aagataaaat tcatcacctt ttctgaggca301 ctgaggctga gtaaatattg actcacccca gcctctctac tttaaggtga acagatgtgg361 attggctgga gcagaactgt ttgcccctgt ggactcacca tagttatttt catcccccaa421 gtcaggttct taaatcggcc cagtgttgac agaaaaaaaa tgcctcctct cccgggggaa481 caggnataaa cactgggtat aaaaattaaa aggncttatt ctgggacttg ccagcttcan541 ttt
21022211596212DNA213Homo sapiens400221 ttttttcttc agctccactt gagtaattaa gtgatcatat tgttttcact ttataaaaat61 actattaaga tttgcatctt aatagtatta agatttgcat cttaatagta ttaagattta121 catcttaata gtattaaggt ttccatattt ctacaaaata aatttgtagt ttcatactgt181 ttcctggctc ctctgtggaa tcaggtcatt taaggctact ctgctgttaa aaatgatgtc241 ttaattttta aaagatattc cagattctac tgttactttc ttatttactc ctcacatctc301 agagctggtc atcttcagca aaataatcca aaataatgaa caatcttatc cattttactt361 acaacagaat tcactgaaaa actaaaggaa aatgcactac caggacactg agaagaatat421 gatgcatttc tttatgatgt aaattgaaag cagctggaag ttagctatgt aatgaactcc481 cggaacaaat acatgagcaa ttgagtcnca acgatgtcac ctttacagag tccccataaa541 gttttctcta catgtcatct gcggtcnata caacaggagc gcggccactg agaagg21023211547212DNA213Homo sapiens400231 tacaagtaac attttactga ggaactggct acatttttct tgttgtaaaa tgaaaacgtt61 tactggaaac atgaaccaca gtgcatgaaa aataaatcag tggataatgc atagagatca121 ctcacttctt catcttctag gaaagcaaag ctggtggcag ggaaatagga aaaagagcag181 ttctctttat cggagtgaaa agaatgagaa cagaagaacc cgcagcctag taggaagagc241 tctcatcatc ctgatttggt ttggaaggct ttacaaaatt ccaagggcat ttccagacta301 gatatttgcc cacaaagtcc tgaccattct caactcattg acccttcttg aaaacagtgt361 tccttcacca atccagtcat ctctgtttta tctgttcatg caacctgtga ctagcaagaa421 tgaatgaaat ccaccataat catgaagttt agcccagagc ttcagtctct tcttggatat481 atggtgcatg tcctctgncc ttcctttggt agaacccatt taaaagggag ncatctagac541 ggtatcc21024211373212DNA213Homo sapiens400241 ttctgggggt ttattttacc atttacctct gacaggatgg ctgaagagga atcatcctgg61 gagttggtta taatattatt tatttaaaaa tatttatcat ttattaagta cttcttaggt121 gacaagaacc ataagagctt tccatacatt gtttcattca atacttacac aattctaatg181 ttatcnccat ttttcagata ggaaaatgaa gttaatcaga tgttgagctt agatttgaat
241 cgaggactat ttaatgccaa agcttaagat tctttaagtc acacacacac agacacacac301 acacacacac acacgacgtn gtttaaattt gngtttccaa ggaaaaagaa aacatgaact361 atagaaaaga aaa21025211603212DNA213Homo sapiens400251 tagaaaataa aaactttatt tttttcaagt ttataagata gttcccatta catataacat61 tacggtcacg gattctacag ccacaaatgc ccgcagtcac ataaatatat ccaatccaat121 caatgccttt tcctgctaac agaggcatct gaagttcaga gggagagtcg cattttgagt181 agaagtcgtc cttaatggga gggctcctgt cagtgcatta ggaactagcc aaggagcctt241 gcttgccaga gctgtctgac tcagaggaga ggaagggaca gatggcctgc tgactggggc301 tgaggcagaa cttagatttt ctctcttgtg gtttaagata ttttagaatc tcggaattca361 gatcctatag tgtgaatatc tggggagttc taacttctgg gatgaaaaag gaaaccaatt421 tagtggtaag aaatagaagc ctgcttaaga gggaccctaa ctgcctcctt gaggagtaag481 gagtcagagg aagaccccta agctcaccat tccctggccc agaccattgc tctaccccat541 actcctctcc cctgtgggtc agtgacactg acancatcaa ggagtaacat ctagagccca601 ntg21026211560212DNA213Homo sapiens400261 gctgatgtcc aagttattta ttaacagagg ggtcatcaca gttgttggtt atattcacag61 actcagctgt gactataaat gctctccaac ctgctcaggg tctttaatga atggaagtgg121 gctttgtgag actaaaaaca atttggaaag ttcatgaaga ttacactgag gccctattga181 gaagctggaa gtgggagcaa taagatggcc ctctgagagc aaaaacatag gagcaatacc241 aggtatagat gggcagacca gcagagatgc ctcatgggac cacaaagcag caaggccatc301 agggttctca gaagaaagag agctggcctg atgtggggga acagggcaag gagagtccct361 gggggatacc attggcagaa cccacacaag cccccggcag ctgggttgca ctcatgggag421 ctggggagaa agagtgttaa anccaaacca ggtcctggcc ctcacaaggn ttggaacagt481 cttagtggga aggtnaccga aaaaccaacc aatccctaga accacttttn catttttcag541 gtggacattc agaccgattc21027211646212DNA213Homo sapiens
400271 gtatttaaat acttttattt tcatttcaca aaaaaagcaa cagtgtttgc agctacacat61 gcctttcagt cagtgctggt catatgcaaa acaagttatc agaaatataa agaaaaataa121 gccttttccc tttacaagtg aaacaacaca atttgcatat acaatattat atatacatat181 tttcaaaatg tttcacaaaa gacagattat gatacaaatt tataattcca cccacaagct241 cagactcaag gaattgaaga tctctatagg tgtttaaata taaaaatgta atacctttac301 aagtttccaa gtaactactt tccaaagtat ttgaaagagg ctattcttca cagcaacatg361 gaacacttgg ctttaatcaa gacagctgta aaactaagtt ttgtctcaat gtgagtttgt421 tgattaatgc aaatgctcca attatggcct aattctcttt tatatacaca tacatacaca481 gcgtattatt gtactgatgg tttacaagtc caatttctgg agctttcctt cagctaagcc541 agacctttac ttccttgagt attttaaatt aagaacgttc agaaatatcn ctaatgncta601 cnggtgttta cntccaaaaa gggncccttt tcttgatttt tggcaa21028211616212DNA213Homo sapiens400281 cacgccttca tatttacact ttgcctttac tttacagttc agtgccagaa atatttacag61 aaaatacaat aagaaaaaat catgtcttgg caacatactg aaattcatac aaactgtata121 tatgatttca agcaaataat gatactgctt attttttagc cacaactgcc aagcccttca181 gaggagaaag atgtcttggt cactataaga gatgaggact tgggaaactt ggtttgcttc241 aacaagtatt tactgggcgc ctactatatg caaggcactg tggaatccca aattatacct301 tttccctcac cattaaaaaa agaaaagcat ctgaagaaca tgagttgata ttctgagaaa361 cacactagaa gtacatggct tctttagcta ctaagaaacc caccgtttga ttccacatcg421 tacggataga ccacctccaa tctctccaac tcaggaaaag aggctgtctg gaaatcttaa481 aacagaccat ctgactccaa tgtctttctg taaacacggc atcagttctt tcggnagaga541 gaaccgaatg ggcttaaatg tgatagagac tactgccgca gcntgtcatt anctccatac601 aggttctntt tatgtt21029211583212DNA213Homo sapiens400291 acatatttac ttttatttac attagtaagg ctgtaaatta tgaatgtaaa tgtgcttagc61 tagaacaaat attgctcaga ataaaaaaga tatacaaata taatgctaat atacaatata121 ggctatcata aaaatttgta tttgtgtaca cacatacata attggctaca ttcgacaatg181 gggttcattt gattcctccg tagtaattag aacaggagac ttaactgatt acagccatga241 tgcggttact taaaaacaca cacacacaca cgcacacagg cacaggcaca cacnngcaca301 ccccacataa ataagtttgg gaaaatttta aattttctca cttaggactt ttcaaagcca361 tctcaaagga gcttcctcaa aacatttaat gttggcatca ccccacaagt actcagaaat
421 aagtgttaaa atagaaatcc aatccagtaa tgggaaactg acttagatta tctatttgtt481 tgtatattta tttgtcatct atctctctta ttcactggct ctattttcaa catctacaat541 agtgctagta atgaatacac agcgtacaca atgtgctaca cat21030211562212DNA213Homo sapiens400301 tcttaatcca aaatacttta ttatgggtaa tactcattta atatttattg gtaatcactg61 agagccaagg taaagtgatg atccaaatga attgaatgtc atgatttaaa taggcaattc121 tcaaattagg gggtttgggt agaaaaataa tatcaaaaag ttcttgtagt taaaaaaaat181 caatatgctt aacatgccta tttattttct taaactacag aaagcaaatc tcacatgctt241 tgcttgtaag aatatgcaaa aaaagtaaat gctgtattaa ctgaaaaact ctaagtacta301 tattttaatt tcgtgaatga tatgggtgat gtcatataga ctgctatttt cagtacatta361 taaaattacc aaaatgttca attttctacc tttaaaaagt aggaaaatat ccacttggca421 accaaccatc aaattaataa ctgattcnag taaganttat caccatacta aaagtagccg481 atgcnagttg gaagagaaac agcctatatg cagtatctgc ccccaattac ttattatttc541 ccaaagggaa aacnggggtg gt21031211404212DNA213Homo sapiens400311 tttttttttt tttttttttt tttttttttt tgttccaaaa cgttttaatt atcangaata61 tcataagtca tacaaaaaaa taaagaataa tacaaccaac actcatgcac ctgccatcct121 atagcttaaa catactatat cacatctgac tattacctag tctgtctttc cccaattagt181 gatgcctcta tagtgagatc tataaatgtt ggttctgttt cttttgtttc tgaaactcta241 gaacctgttc agtacatatn gaataaaaaa gcaacttttc actgaaagag aaggataagc301 ggacatcttt caacagcaga aaatgtatat cactgcctgt tccttctcca nccacaaggg361 ctgaggaggg gatgaaggct ggactctcaa tgggggagaa gtca21032211319212DNA213Homo sapiens400321 tttttttttt tttttttttt tttttttttt ttttttttga cttcctaaat tttttatttt61 aaaaatgccc agtttattta tttgctttgc ctaagatagc tgcattcatt attacagggt121 caggggactt gtaaaaacaa atatcaatta ttataaataa tcncagaaaa caaataaact
181 ggaaaataaa cagcggttac taatttaagg ttttaaccct taccccaacc acccaaaaaa241 agtagccatt tttaaaataa ngattaagag ttggnggaat ttaagggaaa tgccatccng301 gtaatgttac cnaaatact21033211541212DNA213Homo sapiens400331 gcctgcactt tacctgagag ttaccagaac tgccttgaac atatggatga attaaactac61 ataacacatc acccatcttg attcttacaa gttatagacc tcacagtagg cacttctaca121 gtgccaatag cattagcttt ttgtggggta taactgtgtg ggcttcttta atgaagaatt181 tctcttatac cattaagcag gaatctctca aagttccata acaaagacaa attgagtttt241 gatgtgaatt aagtccatgt gctgaattat caattttaaa gttttcctaa ggtattttga301 atgagcaatt cttataccaa agtgaaataa taaactcaac aaaaagaaat ggtataatac361 ttttcctgag gctcatttta ctgattatgc tgagtccaaa tccatgctca tcaagataac421 attactgtat ttttctggaa aaagcatcta ataagtttta aattattgat aatactggnc481 atacatctct accacactgc tgagcatttc taaagcattt ttaaaaattt ggagataggt541 a21034211527212DNA213Homo sapiens400341 tgagctttca aattatttat tttaggtttt attgaaactg aacaaacaaa atattattca61 taaatctatt ataagacaga gaaaaactgc tcaattctgg gnggggtgga agtaggaata121 ggggtacagc tctgtccttc gtcattctac ttatgcataa agcatgttct attgaattcc181 ttagagtctg gacagcacag tttgaaaaac cttcagtgaa atacaaagat cactagatta241 gaaatcgaag gatttagaat cccagttctg ctatcagcca gctgtgttac tgaacctcta301 tggtacagat ttcctcacct gttaaataag gagaatgaac taggtctctt tcaggtcaaa361 tatttgagga aaccattagc aactacatgt aaatcnccaa actcctatac tttatttggt421 tcctactgcc aaataagatg tttccngggg taaatccaca tgcccacctc aaagttctaa481 gtgtcnggag atttttcctt taaccctaag aattttaagg nggtcng21035211848212DNA213Homo sapiens400351 actaattgtg attcccagtt ctgggtgaat aaaaagtttt tgttttaatg taattgtgat
61 caacactatg cttgaagaca tagacaaagc tatttctacc accatgatgt ttacaaaata121 tctggtattt acgcctatat ggccattaaa aaaactagat ttgtggataa caaaccacca181 agttttaatt gtttttattt aataagggag aaataagaca gaaaaagaga attaatactc241 tttttcccag actgagaggc gggcttctaa gtaaaggtat gtgaaaaact gagtagaggc301 ttgtagtact aaaatgaacc tccaaaattt attcttttct ctgaggattt atctcagctt361 ctctgtgtaa ttggatatta acacatctta aattttttaa agagatgggg tctcgctgtg421 ttgctcaggc tggagtgcag tggttactca caggtgcagt catggcacac tgtaacctca481 aactactagg ctcaagcaat cctcttgcct cagcccactg agtagctggg gctatatgca541 tgtacgacca ctggcatgct taaatcttct aaggagcaac acatgtctct actcatttac601 taccattaga tattagatga gtcatttact aatgactgct catggtgtct ctttattctg661 gatatcagat ccattacagc acgccacact aggatttctg cgccacacta cgggggggtg721 gtcgttgttg gtaggtgtgt tcaccacgca ggcccatctt tggaaaaccg cattaagtgt781 ggggacgtct gggtttctcc gtggtcctgg cgtggccttc gttggttaat ccgttcgggc841 cctcgacg21036211354212DNA213Homo sapiens400361 aaaatgaatg tgaagatact gtttacagct tttgttaatt gcacttttca tcactctgat61 caatttcttg taaagtgcta tgggaattat ttttcctgtg aatattaatg attttactac121 tgcctccgaa gttttaaaaa aggttctgta gacagaaaac agttgttgca accanaaaca181 accaaaaana gaaaaaaagg gaaaactggc ttacctttct cagatgaatt aaatgatttt241 aataacttcc caattaccag ggttataaca ttgctttctt cagttattaa tacagtcaaa301 ttaattttaa caatataggc acaattcatt tacaanttca gtacagtagt ccag21037211586212DNA213Homo sapiens400371 tttaacataa aaaaataaat ttattttgag tctgaaatac tgaagaacaa gcatacagat61 aaatagtaca aagaacaaaa attagaacat gagtaatgac ttaagacaca ggcatttttc121 tagctattgc ntacagacac atttttacac acaaacatat tttttaaaga catctctcca181 acattctcaa aaggcaagag ctgtatttgt gacatttgta ataaatgcaa cagcttttga241 aacatccagt ttctttccta agtcatttga ttaaaattca cacaagtgat gattacctat301 tccattntct ganngntacg acatacagtc atgtttcgat caacaattga ccacatatga361 cagagatcct ataagattat aatggaactg aaaaattcct atcacctagt gatgccacag421 ccattgttac attggtagca caatgtatta tctgttcnat gtttagatta cacagatacc481 attgtggtta cagttggcct acagtattcn atacngtacc atggcggtac aggtctgtag541 cccngaagcg acggactttg gccatntgcc ccnggtgtgc ngtggg
21038211498212DNA213Homo sapiens400381 accaaactgt tacatgtggn tggccccagt aagctgacct ggttgccatc ttgagaccag61 acttaaatcc ccaaaaccaa atccacattt caagtatcag tgaacaggta atttttctga121 gaattttgaa aggagttaga agagagcttt tccttggagt cacacaaaat tacttaataa181 aacatcctaa ttagangttt ctgtcctgcc ccctagttcc taaagtcccc tacagaatcc241 agaagccccc tcccacatct ctagggtcct gcctctctcc ttcccttatg ctgagcaaat301 caacttctgg caggcagaga acaaggttga cagatggtac ttgctggata aatacttttc361 tcctgtcaca gtattagggc tcagaaaatg ataccccaaa atatggtgnc ctggcatcct421 gagncacttt gnaccaagga cantggaagg nctcgaagtc aagtctgtct ngatcttctc481 ctgancttct ttctcntg21039211507212DNA213Homo sapiens400391 attaaatggt gtttggagga aggtcatttc agaaggccca gggcangagn tgagtgtcta61 aaacttctaa tctggacttc tattgaagtt tggcagtaag ttaaacagga agttgaaggc121 taccatatgt aaatccagtt gttgcctgaa atcagtcatt tcaagggagt atcttgaaag181 gacacagact gggaaacagg catgagaaaa aaaaaaggaa gtagacagac atttggctag241 tatgtgcctg gaccacttta acactaaaga ttagaaacct agggctctga gtaagaatca301 tttgaccagt agctaaagtg agtcatggcc tgcacagaac ctagggcaca catgttgaag361 tcaggatgtt gagaacatcc aggcttacct aattttgcca aggccaggga gttgctgctt421 ccagtaaggg aattgtgatc cnggaatttc ncgggctcat tttcccagcc ttttcccaac481 cnggtgcaat caaggtcatc ccgtgta21040211427212DNA213Homo sapiens400401 cactactgta gttggaagtt aacaaaaaag tctacctcca aggaactggc agagtgtgag61 aactgtttat aaaaacttga gaattttctc aatcttgctt cgtttgcact ttcccctgga121 ttcccacatc tgattttata ttcttccttc cttgtgctgt ttcttctctt ccttctccac181 agctcttcac ccatcattcc tttttttcac tgatccttct ttagcaaatt ctttcactcc241 caaaggtcct gaacacttca taattttaga aatgactgta ttttagaata accaattatc
301 ctgaatgagt tttggttctt ttgcttcatt tataaatcag ctgggtttaa tgttatggtc361 tgccatttaa actttctact aaattaagta cttcttactt taccacaatc agccagcaag421 ccagttc21041211547212DNA213Homo sapiens400411 tttttttttt tttttttttt tcaggtagta aattttacac atttttattt ttaataatag61 gngtaaatac cttcccacaa caatataatg aacattacag gaaaattaag gcaacttatt121 atttacagaa tgcctttctg ngcaaaacag gacactatac tcttcctaca cattaaagca181 ttaaagatta aaataacnct aaaaggtact tattatcagn gtctttacng aggcaattac241 cagtgttcag ataaataaat gtcagagcta atccaaatat gaaacttgaa attttcccac301 cattctatac aaattcccac agcaaacaga ggaggttatt attttngata ngtcagggaa361 agactttgca gaggaagtaa catttctgag atggagaaaa ctaggagagg aacaggttgg421 agaacaggaa tccaaaagct gtttttgggt caagagatgc ctactagaca ttccaagtgg481 aaatggtaaa gccggtaaag ggttagagct tacngaagng gtccagtcag tgagagcngg541 tttttga21042211481212DNA213Homo sapiens400421 gatgcaattt tccattaatt atacgtgcac acaaggaagg tggggccagg actactactt61 ttaactttgc agaaagcttc atttttactg tgggttgtgg ttaagttaaa aacatttgac121 tatgccatgt aggcgactcc aacacttcag gaatacaaag ctctgaaaag aggttatgta181 gcaaagctca ttttcattca cattgataat aggtcagaca catttttgaa gaaaaaaatg241 gacagagcgt aaaggataac agagtacaca ttttcatttt ctatgatgaa aaggaatttt301 aaaaattgtc tgttgtacat aaaaactttt tttttaaacc aaacaaccta gaattaaatg361 gagtaaaatg tgagaagccc cccttttttc ctccttcagc agacaaaacc gctgtcaata421 gcttgatatg tgttatcaca gactcttttc tagggtgcac cacgcatata tgctacgtat481 a21043211472212DNA213Homo sapiens400431 ctactactac taaattcgcg gccctcgact tttttttttt tagaaaaact ataggtggtt
61 ttgacccact acactgattt cataaaacac taacaagttg tgatctgcaa gttaaaaatt121 ccaatatgaa attcctctgt cttcctatta ccctaagatt gctaggtctt cgctaagacc181 tttgttatta tgaatagcag caaacagata catacattat agagccaaca acagagatga241 ataacatagc tcagaatttc aggaaatgga cagaccagaa cttaatgctc ttcaaaaagt301 aaaatataaa gctatcctcc cacgtttagt acggagatct aattaaagta accatcttta361 aatgatactt tcaagtattt tccaaccata gtaggctcaa tagttgtact cttttgtagt421 tgttcccttt ttagttgttc tcatagaaag ggattaattc tgaacaatta gc21044211340212DNA213Homo sapiens400441 tcgacctcaa tcaagatacg gctcattcca cccttgggca ggagggcaat gctgatgagg61 ctgtttatag cttcggtgcc tgcgcctcca tgttatttag ttgttgttcc tccgctgttt121 ccaaacaagc agtctgtcca aaaaaaaaaa aaggatcaaa tggaaaactt tataaatact181 ccaaatggcg gaaacaggaa gtggaataaa aatgatgtgg ctcttcacag aaaacatgct241 gtcatttttt tttttctctg taaatataca ccattcattt tcagaaagag gctgtgctaa301 cagaagaatt aaaaaaaaaa aaaaaagtcg acgcggccgg21045211428212DNA213Homo sapiens400451 cgacgccttt tccccttaat tcgcaaaccc ttctttcttt tctttttagc gggctgtttt61 gcctctttat tcactccttc tgctaccatc ttttcctcta ccacatcttc ttcatctgta121 ttagaattaa taggaacaca tcccagaatc ccagaatcac taaaagtgtt gcatcttctg181 ccaggagaga gtgaagaaac tggtacactt agagtacata cactcattct ctttatgtct241 ccacaattcg taccagaatc tgttagtggg tttattactc aagcagaaat acagctgttc301 ctctctactt gcattacctt ttctccagtt tctatgggga atcaaattcc cactggacca361 ataacttcca agccaggtta tttttggagg aaaactgaca gcaatggaga acattcccga421 actctttc21046211543212DNA213Homo sapiens400461 atatttcaga tataagattt cagttctcag tgagtctaag tgacagaagg aatggagacc61 ctcttgggcc tgcttatcct ttggctgcag ctgcaatggg tgagcagcaa acaggaggtg
121 acgcagattc ctgcagctct gagtgtccca gaaggagaaa acttggttct caactgcagt181 ttcactgata gcgctattta caacctccag tggtttaggc aggaccctgg gaaaggtctc241 acatctctgt tgcttattca gtcaagtcag agagagcaaa caagtggaag acttaatgcc301 tcgctggata aatcatcagg acgtagtact ttatacattg cagcttctca gcctggtgac361 tcagccacct acctctgtgc tgtgagggac aatgacatgc gctttggagc agggaccaga421 ctgacagtaa aaccaaatat ccagaaccct gaccctgccg tgtaccagct gagagactct481 aaatccagtg acaagtctgt ctgcctattc accgattttg attctcaaca aatgtgtcac541 aag21047211398212DNA213Homo sapiens400471 agtagactta caaatattct atttcgcatt atattcaaga ctaaacatct tccaaaccat61 attcatgaaa tggtttgatg atatgtgctt tggcggtttt caagaaatat caatcaaacc121 gtaattaaat ttcaacgtat cggctaaaca tccactgagc acctcctctt gcagttagca181 ttagactaag tgcttaagga caagtagttt gatgcaataa attaagaaat acatatttaa241 gacttatatt attcacagaa ttcttggcat agttatttaa gttcctcctg ttgagaaact301 tgaggtttgt gttttctttc tttcagtccc aaaagctccg ttttgagttc tccacgcttt361 ggtggaattt caggtattgt ctgtagcagt tctcccca21048211344212DNA213Homo sapiens400481 aaaaaacatc ataggatact cgacctcact tatactcaag aaatacaaat tgaaatgaat61 actgtttccc acatactgga ttggcaaaga ttataaagat gtgtaaaacc tggtatggac121 attggaaaat caggcattct tgttctgagt tcatttatgc atgcaacaga tagcaactcc181 tgtgttcagg catgtnctaa gcactgggga tacagcactg aacaagaccc ccacctttaa241 agatcatagg ttccaactag gtgaaagctg atagtaaaaa tatatattat ttttgaggga301 ttaagtacat tgaggaaaag caagaaaggt aatggttagg tagg21049211294212DNA213Homo sapiens400491 gagattttca aaataagtca cttattcctg ttaacatcgc cctttttgtc aataatcaaa61 tcaagcaggt tgtgtttttt tatgtaagan ttccaacaag aagatgtaaa ccaccaattc
121 aggattgttt tcactttctt tttttgagat ggggcctcac tctgtcaccc aggctggagc181 tagagtacag tggtgtgatc tcagcttact gcaaccacct cctgggctca agcaatcctc241 ccacctcagc ctccccagta gctgggacta caggcatgca ccaccattcc cagc21050211372212DNA213Homo sapiens400501 gaataagata caagtttatt tatctttcat gtaacagtcc agggctaata gagcagttct61 gccatcctca tcacagggct tctgcctctg ggtccaaagt agttgcccca attcctgcca121 tcacatctgc accccagcca gtggagaggg taagagggca agggtaacac atacatattc181 ttttttaggg cacaaactag aaataataca tgcaacttcc gttcacatct cattggcccc241 aaactttgga caaacccaga tgcaagagaa gctggtaaat tagcctttaa ttgagaagcc301 aagcgcccag aaagcaggga gttagagtat acaaggaaag atcattttta tggntatcag361 tctcattcag aa21051211409212DNA213Homo sapiens400511 aacagtgtta tacaaatgtt aattttttaa ttttaaaagt accatggtta tgangatgtt61 aacattaggg gaagccgggt taagggtata tgggaactct ctgtactaac tttgcaatgc121 ctctgtaaat ctaaaattct tcaaaataaa actaccaaag aaacacaaag aaataaggtt181 ggggctactg tcaacaggat ttgctgatct caccatgtgt ctgttatcta gaagcaagtg241 gctgagtgat aggctgtcct agtaaagacc cagggtggtg ccaggtaggt ggacaccact301 ttgcaaagac taagtgatgt caggnaggtt gctggctgtg ctatgaatgg gacccagaaa361 caacttacag tggttttncc cttcatggct aaaaaacctc aaggttcct21052211533212DNA213Homo sapiens400521 tttttttttt taggcaaagt gttctttatt atcaataaac acaaaccatt aaagaaaaca61 ttgataaatt cgactttata aaaattaaaa gtttttgctc aacagtatta agagaacaaa121 tataagctgc agtttgggag aaaaacagtg gaaatcacca atatgacaaa gggcatgtgt181 gatagttact tgtacttgtc actgtgaccg agcaccaggg tccacggaca tttggccaaa241 catgattctg gttgtgttcc agagagtctt tcagatacga ttaacattgg gatgggcaga301 ctaagtgaag cagattgccc tccttaatag gggtgggcct catgcaatca atcaagggcc
361 aggagagaat taagaggcct aatgggaaac aaatgctttc ctgggtatcc agctttcctt421 ccatcttggg aatttcagcc tccataatct cagaagcaaa ttcatgtata tatatataca481 cacatataca tttcataggt atgtggctaa gattgtattt ttacaagttc agc21053211276212DNA213Homo sapiens400531 tttttaaaca aggataggtt ggggaggtgg gaaaagagtg tcaacaggag aaagggggct61 gggaattctg attctctaaa agagatgagt agtcctagaa agagaacagg ggcacgattt121 gcaggcaata aaatgttagt ctgggctcag gagagctgca gttacacagc cccctccatc181 caggaccgtg atgagtagtt cctcgcaggg ctccctggaa acgtgggtag tagatgaaca241 ggcctccaag cagctgctga cataatcatt gtggcg21054211437212DNA213Homo sapiens400541 ttctattttg cagactttct tttttaaaag caaaataaat tgacatgact tgttcagggt61 taactgtttg gcaggtggat gatctgtggc catccatgat gagatcacct ccctgccccg121 ctggccccca gcctctagaa gtcagggctt ctgaggccca gaagctcagc gccacacctg181 ttgaaggcca gtgatgtcag agttactctt ccttcctcca gcagcactga cagcagttta241 ttgtacgcaa tttctagaac tcagatgttc tagaaggaag caaacatatt ctgagatcac301 agactatgac tatgctctca gaatatgttc tagaacacct aagttgcaat tcttaaaatc361 aacacagcgt aagactgctt taggaggaag tgatcaagct caaagcaacc taggcatgat421 gtgccttgtt tgtttat21055211432212DNA213Homo sapiens400551 gtttaagagt tttgtattat gtcatacaaa ctgtcttcac atacaagtgc attaaaaaaa61 ttatcccccc ctcctcctcc ccaaacatca cagtagacca attcttgcta atatatgaca121 aagttaaaac atatgtcccc tctgaattct aaacagctaa ttctagacac taacatctgg181 atcttataat ttaccggtct ggtatggaca tgaacagttc cagtgccttt caacttatcc241 ctgaattttt ctcttctcaa agttctaggc agtaatttta atgccctcaa attagataaa301 gctgattact tagagcttat aaattagcta cactttatga cactaagaat agagctatga
361 cttatgcata tcatacctgt caagctacct agcaccacga ataggactaa tattaatgga421 tggactaata ca21056211529212DNA213Homo sapiens400561 tttttttttt tggccacatg tttattttta taaaatatct agaacaggca aatcatagag61 acagaaaatg gattattgtt tctagaggct gggggaagag gaggattaga gtggcagctc121 actggtatgg agtttctagg tagatggaaa tgttctggaa ttagatagtg gtgatgattg181 cacaactctg taaatatact aaaatccact gcatcattta ctttaaaggg gcaaatttta241 tggcttgtga attacatttc aataaaaaga agatgaggaa acctaggata tgtcacaatc301 ccatgccaca aactacttac acttccaata taaaaactat atcaacaata gtttacatca361 cgttcattgc ttgctaaagt aaggtcatga atgtatcaaa atattttata aaagttaaaa421 tttaggagat ccatcatttc tcttttcaat ccatcactga ttcattcaat atatatttat481 gtgtatgcca ggctcttcat caggtaaagt gtttaggaga gatataaag21057211399212DNA213Homo sapiens400571 tcgacttttt tttttttttt tttttttttt ttacatcatt tgcctttttt attttgaaat61 gaaaagtctc acatatttat tactgaaccc acccaactga cgtgttcata gcagattcag121 agaaaaaaca tattcccaat aaaacatttc caacactcca gatagtggtg acattttcag181 cttgatatgg taacgcgatt gtaatgctca cacagcataa atatgtgtgc catctcacgt241 gcaattcctt atagaccccg cttcgttctt ctccaatgtc tccttttgga gtcgtacctg301 attttattac cagttttgat ctgaatccac tgggggaatg gaatgatttt gcctttgttt361 cttggccagg tattgcttaa atctgaaagt cccgtgaga21058211451212DNA213Homo sapiens400581 ttttgtattt tggattattt gcattatata cttactggtt gagcattcca aatctgaaaa61 tctgaaatct tccagtggcc atttcctttg agcatcagtc atgttggtgg gtcagaaagt121 tttggacttt ggagcatttg aatttctgga ttagggatac ccaaactgtg tgtgtgtgtg181 tgtgtgtgtg tgtatcctca tccttttcaa ctcttggaaa atattccaga gtataaataa241 gccacctttt ctttcattgt accctaactt tggaaactca gtctgtttta aattgtttaa
301 aattataatg ttacagcaat ttctgtacat atacctgagt acacatttat tagtattctt361 tgagtttttg agaaatagac tgaatatatt taaaattttg atgatgtcaa ttttcctttc421 aaaaaggccc tatagtcagg tgtggtggct c21059211493212DNA213Homo sapiens400591 ttggggaaca ggttaaagct tttattttca ggttacagtt tttgatgccc ctttctgcta61 ctccacgggg tacttcaatc atccgcttcc gcacatctct tgactcattt ggcagaagag121 cccccacggg aacccttcgg cttcttttct gacacctcac gtacttgcag cctgaaggcc181 ccgcaggcga cacggttaag tggatgtttg gtggaaccct ccctgggccc acactccgcc241 cctccccagc aggccgcgca acaattccca gggcccaccg aggggtgctt agctcggagg301 agggggagtg acgtcacagc gatgttcaaa acaaggcctc ccctggcccc accatagaaa361 agcaccagga aggggaatgt ggcgagcact ccaggaaatg ctcggattgg ggaccaggtg421 acactgtagg aatgtgtgtg ctgggggatg ggatggtgtc ctaaatatgg gtcaccagga481 gcagaaggag ctc21060211447212DNA213Homo sapiens400601 ttttttttaa gaaagtattt tatttttgtg tctcaacaga tgaaattcat aaccttgttt61 tctgataaga caattcaaac atacaaatca attacaacaa tgtgcttatc agctcccctc121 ccacccctat attttaatgc aactgacagt tttgaaggac accaagacaa tagggcttag181 ctaaacaata cggcaattaa aaatggccct ctaagaaaat aattacaata gttgttgaaa241 agaatttgtc ctttgagcaa aacagactga aaaggattat catgaaatga ggagaattat301 agcttctctt ccaataaagg agaaaagggg atgtatgtta aaacaatgat acagaaagct361 ggggagtcag actgaaaaca gaaagcccca ggcaataaat gtctccaata aaatgcatcc421 ccttgaagat atccattcaa actcttg21061211463212DNA213Homo sapiens400611 gctattgtaa atggggtttt ctcttcgatt atatctcttt ttttttaaga ctagtcaagc61 gcagtagtaa caagtggaga aagactagaa caaggagttt aatctataac tgactgaaaa121 gtcaattgag ataactcagt tggacctttg gaccagcctt ccttatacct tctaactcat
181 tattgtttac atttatgaag gcttttgatt tttccatgtt aattttatca tacctattac241 cctattgagt tattttactg agttgggatt ttttaacgac tttattgaga aatcatttac301 atgccataaa attcactgat tttaaatgta ctattcaatg ggttgaagta tatttagaga361 gctgtacagc catcaccaca atgtaatttt agaacagttt catcacccca aaaagaaacc421 tttaggccca ttaggagtta ctccctatcc ctaccccatc tcg21062211441212DNA213Homo sapiens400621 tcatgatcac acagaacttt tgtatctcct aaagcaaggt taaaaatcct tggattttgc61 ttgcctttta tacatcaata ataatttttt agaaaaactg tatttttaat tatgtcacaa121 aatataaaag tcttcctttg ttgatctcaa aacaaatcct tctaattttt agtttattgt181 tttctttttt tttttttttg agacagggtc tcattctgtc accaaggccg gagcttaata241 gtgtgatctc agtgatcttc ctgtctcatc ctcccaagta gctgggacta caggagcatg301 ccagtttttt ttttcttttc tttttttgta aagacagggt atcaccattg ttacccaggc361 tggtcttaaa ctccaaactc ctgggctcaa gctatccgcc catttctgtc tctcaaaatg421 ctgagattac aggtgcaagc c21063211481212DNA213Homo sapiens400631 tttttttgag acaaagtctc agcttgtcac ccaggctgga gggcaatggc atgatcttgg61 ctcactaaaa cctccacctc ccaggttcaa gtgatcctct tgcctcagcc tcccgagtag121 ttgggattac aggggcctgc caccatgccc ggctaatgtt tgtgttttta ctagagatgg181 ggtttcatca tgttggccag gcttgtctcg aactcctgac ctcaggtgat ccactcacct241 tggtctccca aagtactggg attacaggca tgagccacca tacccagcct ttttttcttc301 taggtaccag cttttattta tcagattggt ataaatgtta gaaagcgtgc aatgaaatgg361 gcattttcac agtcgtggca gaaagtataa ttatctttga ctttctagaa agcagtctgg421 cattctagaa acttgcctaa cctcttccca tttagacaag atgaattctg acagggccag481 c
權利要求
1.一類在人類肝臟中表達的表達序列標籤的序列，其特徵在於，它包括(a)SEQ ID No.1～SEQ ID No.63所示的序列；(b)SEQ ID No.1～SEQ ID No.63所示的序列中每條序列的互補序列；(c)與SEQ ID No.1～SEQ ID No.63所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數條的組合。
2.如權利要求1所述的表達序列標籤的序列，其特徵在於，所述序列包括具有SEQID No.1～SEQ ID No.63所示的序列。
3.一種探針分子，其特徵在於，所述的探針分子含有權利要求1中所述的序列中約8-100個連續的核苷酸。
全文摘要
本發明公開了一類在人類肝臟中表達的表達序列標籤的序列。利用本發明的在人類肝臟中表達的表達序列標籤，可以方便的尋找出在人類肝臟中表達的相關基因，從而在研究肝臟疾病的致病機理以及開發治療肝臟疾病的藥物中發揮重要作用。
文檔編號C12Q1/68GK1955293SQ20051003082
公開日2007年5月2日 申請日期2005年10月28日 優先權日2005年10月28日
發明者黃健, 韓澤廣 申請人:上海人類基因組研究中心