在植物中產生二倍體配子的TDM基因中的顯性突變的製作方法

2023-10-09 23:36:49 1

本發明涉及：引起在植物中產生2n配子的TDM基因中的顯性突變；包含所述突變的植物；以及它們在植物育種中的應用。本發明還涉及這樣的植物，在該植物中，TDM基因中的顯性突變與以下基因的失活組合：參與植物中減數分裂重組的基因，和參與減數分裂期間著絲粒的單極取向的基因。這些產生未減數分裂配子的植物也可用於植物育種。

背景技術：

減數分裂是有性繁殖的真核生物(諸如，大多數的開花植物)生命周期中的關鍵步驟。

在正常的減數分裂中，染色體首先複製，產生成對的姐妹染色單體。在該輪複製之後，進行兩輪分裂，稱為減數第一次分裂和減數第二次分裂。在減數第一次分裂期間，同源染色體重組並分離在兩個細胞隔室(compartiment)中，這個兩個細胞隔室各自都包含染色體的一個完整的單倍體內容物。在減數第二次分裂中，由減數第一次分裂產生的兩組染色體進一步分裂，使姐妹染色單體分離開。因此，由該分裂產生的四個孢子是單倍體(n)，並且攜帶重組的遺傳信息。

相比之下，在二倍體細胞的有絲分裂期間，染色體進行複製，姐妹染色單體分離產生子代細胞，該子代細胞是二倍體(2n)的並且在遺傳學上與原始細胞相同。

已經示出，由減數分裂期間的異常現象得到的異常配子可用於幾種感興趣植物(包括作物)的遺傳改良(供參考，參見例如，Ramanna&Jacobsen,Euphytica 2003,133,3-18)。具體地，2n配子和未減數分裂配子(apomeiotic gamete)可用於產生多倍體植物，或用於使不同倍性水平的植物，例如四倍體作物與其二倍體野生近緣種雜交，以便在植物育種方法中使用它們的遺傳多樣性。它們也可用於遺傳作圖的方法。未減數分裂配子對於產生無融合生殖植物(即能夠從胚珠的母體組織形成種子的植物)也是感興趣的，從而產生作為母本的遺傳克隆的子代。

2n配子(也稱為二倍體配子)是具有體細胞染色體數，而不是配子體染色體數的配子。引起2n配子形成的異常情況特別包括異常胞質分裂，第一次減數分裂或第二次減數分裂的遺漏(skip)，或紡錘體異形(供參考，參見V Veilleux,Plant Breeding Reviews,1985,3,252-288,or Bretagnolle&Thompson,New Phytologist,1995,129,1-22)。這些異常情況導致不同類別的未減數的配子。例如，第一次減數分裂的遺漏產生第一次分裂恢復(FDR)的配子，而第二次減數分裂的缺少產生第二次分裂恢復(SDR)的配子。已經報導了在各種植物物種中能夠產生2n配子的許多突變體。然而，還未對這些植物中參與二倍體配子形成的突變進行表徵。

未減數分裂配子是遺傳學上與原始細胞相同的配子，保留親本的所有遺傳信息。未減數分裂配子的產生是無融合生殖的關鍵因素之一(Bicknell&Koltunow，Plant Cell，2004,16，S228-45)。雖然，超過400種植物是無融合生殖的，但這些植物中包括很少的作物物種。此外，通過雜交來引入這一性狀的嘗試已失敗了(「The Flowering of Apomixis:From Mechanisms to Genetic Engineering」,2001；Editor:Savidan et al.；Publisher:CIMMYT,IRD,European Commission DG VI(FAIR),MEXICO,2001.Spillane et al.,Sexual Plant Reproduction,2001,14,179-187)。

迄今為止，已經證實了只有少數基因與2n或未減數分裂花粉的形成有關。

AtPS1(擬南芥平行紡錘體，Arabidopsis thaliana parallel spindles)的失活產生二倍雄孢子，從而產生具有重組遺傳信息的可育的二倍花粉粒和在子代中產生自發三倍體植物(WO 2010/004431；d』Erfurth et al.,PLoS Genet.,2008,4,e1000274)。

TAM(緩慢異步的減數分裂，也稱為CYCA1；2)或OSD1(第二次分裂的消失)的失活導致在減數第一次分裂之後過早地退出減數分裂，從而產生具有重組遺傳信息的二倍體孢子和SDR配子(d』Erfuth et al.,PLoS Genet.,2010,6,e1000989；d』Erfuth et al.,PLoS Biol.,2009,7,e1000124；WO 2010/079432)。

SPO11-1編碼植物中進行有效減數分裂重組所必需的蛋白，並且它的抑制消除重組和配對(Grelon et al.,Embo J.,2001,20,589-600)；而Rec8(At2g47980)編碼著絲粒在減數分裂期間的單極取向所必需的蛋白(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)，並且它的抑制改變染色單體的分離。Atspo11-1突變體經歷不平衡的第一次分裂，隨後進行第二次分裂，產生不平衡的孢子並且是不育的。Atspo11-1/Atrec8雙突變體經歷有絲分裂樣分裂(而不經歷正常的第一次減數分裂)，隨後進行不平衡的第二次分裂，產生不平衡的孢子並且是不育的(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。

在osd1/spo11-1/rec8三重突變體中，spo11-1和rec8突變的存在導致有絲分裂樣的第一次減數分裂，並且osd1突變的存在阻止發生第二次減數分裂。因此減數分裂完全替換成有絲分裂，且不會影響隨後的有性生殖過程。因此，將osd1/spo11-1/rec8突變體命名為MiMe(有絲分裂而不是減數分裂)(d』Erfuth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432)。由MiMe突變體獲得的孢子和配子在遺傳上與原始細胞相同。

迄今為止，能夠產生2n配子或未減數分裂配子的植物的工程因此限於有限數目的基因。

因此，為了增加可以經修飾以產生高頻的2n配子或未減數分裂配子的基因的數目，需要參與植物中的這些配子形成的其它基因。

TDM(三次分裂突變，THREE-DIVISION MUTANT)基因，也被命名為TDM1、MS5(蛋白雄性不育，PROTEIN MALE STERILE 5)或POLLENLESS 3編碼屬於植物中保守的小蛋白家族的蛋白。擬南芥(Arabidopsis thaliana)的TDM基因的序列能夠在TAIR資料庫中以登錄號At4g20900來獲得，或在GenBank資料庫中以登錄號NC_003075.7來獲得。它編碼434個胺基酸(aa)的蛋白，其序列在所附的序列表中表示為SEQ ID NO：1。

TDM基因被描述為在減數分裂結束時退出減數第二次分裂所需的基因。TDM突變導致多分體(polyad)的形成，以及由於在正常的減數第一次分裂和減數第二次分裂後進入異常的第三次減數分裂而引起的雄性不育。據表明，tdm突變體攜帶突變，該突變產生編碼在其C末端缺少305或112個胺基酸的截短的TDM蛋白的基因(Bulankova et al.,The Plant Cell,2010,22,3791-3803；Cromer et al.,PLoS Genet.,2012,8,e10028652012；Glover et al.,The Plant Journal,1998,15,345-356；Ross et al.,Chrom.Res.,1997,5,551-559；WO/9730581)。

與此相比，如本文所示，本發明人已經確定了TDM基因中的顯性突變，該顯性突變使得在減數第二次分裂之前提前退出減數分裂，從而產生具有重組遺傳信息的二倍體雄SDR配子和二倍體雌SDR配子以及二倍體孢子。此外，他們已經表明，在spo11-1 rec8雙突變體的TDM基因中引入顯性突變產生了MiMe突變體。

本發明人因此鑑定出了在植物中參與2n配子和未減數分裂配子形成的另一個基因。

與參與二倍體配子產生的其它突變相比(這些突變是隱性的，因此需要初生突變體(突變雜合)的額外自花授粉步驟來獲得突變純合的植物)，TDM中的顯性的突變不需要這一步驟。攜帶TDM基因中的顯性突變的初生突變體能夠產生2n配子。

技術實現要素：

因此，本發明提供了一種用於獲得產生第二次分裂恢復(SDR)2n配子的植物的方法，

其中，所述方法包括提供基因內包括顯性突變的植物，所述基因在此指TDM基因，編碼在此被指定為TDM蛋白的蛋白；

其中，當所述植物為芸苔屬Brassica spp時，所述蛋白與SEQ ID NO:1的TDM蛋白的第1至286位胺基酸殘基具有至少75％序列一致性；或者，當所述植物不同於芸苔屬Brassica spp時，所述蛋白與所述殘基具有至少30％序列一致性，並且所述蛋白的前60個胺基酸包括在此被指定為TPP/Q模體的模體X1X2X3，其中，X1為蘇氨酸(T)，X2為脯氨酸(P)，以及X3為脯氨酸(P)或穀氨醯胺(Q)；以及

其中，所述顯性突變使所述植物具有產生SDR 2n配子的能力。

在下面的描述中，使用標準的單字母胺基酸代碼。

TDM基因和蛋白質序列能夠在公共資料庫中獲得，諸如但不限於，Plaza資料庫(http://bioinformatics.psb.ugent.be/plaza/)和phytozome網絡門戶http://www.phytozome.net/(phytosome v9.1)。

在使用具有默認參數的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)進行序列比對之後，對SEQ ID NO：1的第1至286位殘基計算TDM蛋白的蛋白序列一致性；使用Bioedit 7.2.5由該比對獲得百分比一致性(Hall,T.A.,1999.BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl.Acids.Symp.Ser.41:95-98)。

每個物種具有一個或兩個TDM基因，通常具有一個(圖1和圖2，以及表I)。取決於物種，TDM蛋白由約400個胺基酸至約850個胺基酸組成(圖1)。根據圖1中所示出各種被子植物物種的TDM蛋白的比對表明，TDM蛋白的前半部分是保守的。特別地，所有TDM蛋白的前60個胺基酸都包括保守的TPP/Q模體(圖1)。

在使用具有默認參數的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)進行多重序列比對之後，計算各種被子物種的TDM蛋白與SEQ ID NO：1的TDM蛋白的第1至286位殘基的百分比序列一致性。使用Bioedit 7.2.5由該比對獲得一致性矩陣(Hall，TA，1999.BioEdit：a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl.Acids.Symp.Ser.41：95-98)。結果示於下表I中。

表I：TDM蛋白的序列一致性

*除了黃瓜Cucumus sativus、番茄Solanum lycopersicum、藍花耬鬥菜Aquilegia caerulea、菜豆Phaseolus vulgaris、柿樹Prunus persica、雷蒙德氏棉Gossypium raimondii、大豆Glycine max和可可樹Theobroma cacao來自於http://www.phytozome.net/(phytosome v9.1)之外，所有的序列都來自於Plaza資料庫(http://bioinformatics.psb.ugent.be/plaza/)。

與SEQ ID NO：1的TDM蛋白的第1至286位胺基酸殘基具有至少30％序列一致性的序列與SEQ ID NO：1的TDM蛋白的第1至286位胺基酸殘基具有至少50％的序列相似性。因此，除了芸苔屬Brassica spp.植物以外的其它植物的TDM蛋白可替代地被限定為與SEQ ID NO：1的TDM蛋白的第1至286位胺基酸殘基具有至少50％的序列相似性，並且包括在該蛋白的前60個胺基酸中的TPP/Q模體。

根據本發明產生的SDR 2n配子可用於2n配子的所有常見應用中，例如用於生產多倍體植物，或允許不同倍性水平的植物之間進行雜交。它們也可用於遺傳作圖的方法，例如WO 2006/094774中所公開的「反向子代作圖」的方法中。

根據用於獲得產生第二次分裂恢復2n配子的植物的方法的優選實施方式，當所述植物不同於芸苔屬Brassica spp時，所述蛋白與SEQ ID NO:1的TDM蛋白具有至少35％的序列一致性，並且逐漸優選地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性；或者，當所述植物為芸苔屬Brassica spp時，所述蛋白與SEQ ID NO:1的TDM蛋白具有至少80％的序列一致性，並且逐漸優選地，具有至少85％、90％、95％或98％的序列一致性。根據用於獲得產生第二次分裂恢復2n配子的植物的方法的另一優選實施方式，所述TPP/Q模體位於所述蛋白的SEQ ID NO：1的第16～18位的區域中。

根據本發明，所述顯性突變在TDM基因或TDM轉基因的等位基因內，其中，所述TDM基因或轉基因來自任何植物物種，例如表I中提及的那些物種。因此，能夠產生SDR 2n配子的植物能夠通過TDM基因的定向誘變或隨機誘變來獲得，或者通過遺傳轉化來獲得。

根據本發明的用於獲得產生第二次分裂恢復2n配子的植物的方法的優選實施方式，所述方法包括：

提供在TDM基因的等位基因內具有所述顯性突變的植物，所述植物對於該突變是雜合的或純合的。

TDM基因的誘變可以是定向的或隨機的。在隨機誘變(例如通過EMS誘變)之後，篩選期望基因內的突變體。用於高通量誘變和篩選的方法是本領域可知曉的。作為實例，可以提及TILLING(定向誘導基因組局部突變(Targeting Induced Local Lesions IN Genomes)，由McCallum et al.,Plant Physiology,2000,123,439-442所述)。利用標準技術進行定向誘變，該標準技術是本領域所已知的並且利用同源重組，優選與核酸酶例如TALEN或CRISPR組合。

根據本發明的用於獲得產生第二次分裂恢復2n配子的植物的方法的另一優選實施方式，所述植物是轉基因植物，並且所述方法包括：

a)用包含DNA構建體的載體轉化至少一個植物細胞，所述DNA構建體包括具有所述顯性突變的TDM基因；

b)培養所述轉化的植物細胞，以使在其基因組中具有轉基因的植物再生，該轉基因包括所述DNA構建體。

DNA構建體包含TDM基因，該TDM基因可來自與其將引入的植物相同的物種，或來自不同的物種。

在TDM基因內的突變中，可以基於對於該突變是雜合的植物的表型性狀，來確定導致產生SDR 2n配子的能力的那些突變：這些植物可以形成至少5％，優選至少10％，更優選至少20％，還更優選至少50％，並且高達100％的雙孢體，作為減數分裂的產物。

可替代地，可通過它們恢復擬南芥(A.thaliana)spo11/rec8雙突變體的可育力的能力，來確定TDM基因內，導致突變體或轉基因植物具有產生SDR 2n配子的能力的顯性突變，其中可育的spo11/rec8/tdm三重突變體對於TDM突變是雜合的，如本申請的實施例中所證實的，擬南芥(A.thaliana)spo11/rec8雙突變體用於篩選顯性突變，隨後將該顯性突變引入到感興趣的植物中。

根據本發明的用於獲得產生第二次分裂恢復2n配子的植物的方法的另一優選實施方式，所述顯性突變包括保守TPP/Q模體的至少一個殘基的突變或由保守TPP/Q模體的至少一個殘基的突變組成。

上述突變可以是置換、插入或缺失，優選置換或缺失。

在更優選的實施方式中，所述顯性突變包括T殘基的突變和/或它相鄰的P殘基的突變，或由T殘基的突變和/或它相鄰的P殘基的突變組成。TP是潛在的磷酸化位點。本申請的實施例證實了T16或P17殘基的突變能夠顯著地提前退出減數分裂。不希望受理論的束縛，本發明人認為鑑於這些結果，TDM通過磷酸化來調節，以確保減數第一次分裂至減數第二次分裂的過渡。

因此，突變有利地是消除所述模體的T殘基磷酸化的突變，即，破壞TP磷酸化位點的突變。

所述突變有利地是所述T殘基和/或P殘基被不同的殘基置換，例如T被A置換，P被L置換。可替代地，所述突變是缺失T殘基和P殘基，並且最終缺失所述T殘基和/或P殘基兩側的額外殘基，例如缺失1個至10個，優選1個至5個，甚至更優選1個或2個殘基。

如上所限定，本發明的另一方面涉及包括TDM基因的DNA構建體，所述TDM基因具有使突變體/轉基因植物具有產生SDR 2n配子的能力的顯性突變。TDM基因可來自與其將引入的植物相同的物種，或來自不同的物種。DNA構建體包括可表達形式的TDM基因。優選地，TDM基因位於啟動子的轉錄控制下，該啟動子在植物細胞中有功能。啟動子可以是TDM基因啟動子，諸如所述TDM基因的內源啟動子，或在植物中有功能的另一啟動子。

在本領域中，具有大量供選擇的適於在植物中表達異源基因的啟動子。

它們可以例如獲自植物、植物病毒或細菌(諸如農桿菌(Agrobacterium))。它們包括：組成型啟動子，即在大多數組織和細胞中以及在大多數環境條件下具有活性的啟動子；以及，組織特異性啟動子或細胞特異性啟動子，僅僅或主要在特定組織或特定細胞類型中具有活性；和，誘導型啟動子，該誘導型啟動子通過物理刺激或化學刺激，諸如由於線蟲感染而產生的物理刺激或化學刺激而被激活。對啟動子進行選擇以便在性母細胞中具有功能。

常用於植物細胞中的組成型啟動子的非限制性實例是花椰菜花葉病毒(CaMV)35S啟動子、Nos啟動子、1,5-二磷酸核酮糖羧化酶/加氧酶(rubisco)啟動子、木薯葉脈花葉病毒(CsVMV)啟動子。

可用於本發明的器官或組織特異性啟動子特別包括能夠賦予減數分裂相關表達的啟動子，諸如DMC1啟動子(Klimyuk&Jones，Plant J，1997,11,1-14)。

本發明的DNA構建體通常還包括轉錄終止子(例如，35S轉錄終止子、胭脂鹼合酶(Nos)轉錄終止子或TDM基因終止子)。

本發明還包括重組載體，所述重組載體包含本發明的DNA構建體。通常，所述重組載體還包括一個或多個標記基因，允許對轉化的宿主進行選擇。

合適載體的選擇，以及用於將DNA構建體插入到其中的方法是本領域普通技術人員所熟知的。載體的選擇取決於預期的宿主以及所述宿主的預期的轉化方法。在本領域中，對於許多植物物種，雙子葉植物或單子葉植物，有多種用於植物細胞或植物的遺傳轉化方法。作為非限制性實例，可提及的是：病毒介導的轉化，通過顯微注射、電穿孔進行的轉化，微粒介導的轉化，農桿菌(Agrobacterium)介導的轉化等。

本發明還提供了包括本發明的重組DNA構建體的宿主細胞。所述宿主細胞可以是：原核細胞，例如農桿菌(Agrobacterium)細胞；或者真核細胞，例如通過本發明的DNA構建體遺傳轉化的植物細胞。構建體可被瞬時表達；它也可被併入到穩定的染色體外複製子中，或整合到染色體中。

本發明人還發現，通過使TDM突變中的顯性突變與兩個基因的失活相組合，來得到產生未減數分裂配子的MiMe突變體，該兩個基因中的一個基因對於減數分裂重組的起始是必需的並且選自spo11-1、SPO11-2、PRD1、PRD2(AT5G57880)、PRD3/PAIR1和DFO(AT1G07060)，另一個基因為REC8。

由MiMe突變體產生的未減數分裂配子可以與SDR 2n配子相同的方式，用於生產多倍體植物或用於不同倍性水平的植物的雜交。它們對於生產無融合生殖植物也是感興趣的，即能夠從胚珠的母體組織形成種子的植物，從而產生為母本的遺傳克隆的子代。

因此，本發明的另一目的是用於獲得產生未減數分裂配子的植物的方法，其中所述方法包括：

a)提供植物，所述植物包括如上定義的在TDM基因中的顯性突變；

b)在所述植物中，抑制參與植物中起始減數分裂重組的第一蛋白，所述蛋白選自以下蛋白：

被指定為SPO11-1蛋白的蛋白，其中，所述蛋白與SPO11-1蛋白具有至少40％的序列一致性，並且逐漸優選地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與SPO11-1蛋白具有至少60％的序列相似性，並且逐漸優選地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-1蛋白在SwissProt資料庫中的序列登錄號為Q9M4A2，對應於所附序列表中的SEQ ID NO:29；

被指定為SPO11-2蛋白的蛋白，其中，所述蛋白與SPO11-2蛋白具有至少40％的序列一致性，並且逐漸優選地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與SPO11-2蛋白具有至少60％的序列相似性，並且逐漸優選地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-2蛋白在SwissProt資料庫中的序列登錄號為Q9M4A2，對應於所附序列表中的SEQ ID NO:30；

被指定為PRD1蛋白的蛋白，其中，所述蛋白與PRD1蛋白具有至少25％的序列一致性，並且逐漸優選地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與PRD1蛋白具有至少35％的序列相似性，並且逐漸優選地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD1蛋白在GenBank資料庫中的序列登錄號為ABQ12642，對應於所附序列表中的SEQ ID NO:31；

被指定為PRD2蛋白的蛋白，其中，所述蛋白與PRD2蛋白具有至少25％的序列一致性，並且逐漸優選地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與PRD2蛋白具有至少35％的序列相似性，並且逐漸優選地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD2蛋白在Plaza資料庫中的序列登錄號為AT5G57880，對應於所附序列表中的SEQ ID NO:32；

被指定為PAIR1蛋白的蛋白，其中，所述蛋白與PAIR1蛋白具有至少30％的序列一致性，並且逐漸優選地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與PAIR1蛋白具有至少40％的序列相似性，並且逐漸優選地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PAIR1蛋白在GenBank資料庫中的序列登錄號為NP_171675，對應於所附序列表中的SEQ ID NO:33；

被指定為DFO蛋白的蛋白，其中，所述蛋白與DFO蛋白具有至少30％的序列一致性，並且逐漸優選地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與DFO蛋白具有至少40％的序列相似性，並且逐漸優選地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述DFO蛋白在Plaza資料庫中的序列登錄號為AT1G07060，對應於所附序列表中的SEQ ID NO:34；以及

c)在所述植物中，抑制被指定為REC8蛋白的第二蛋白，其中，所述蛋白與REC8蛋白具有至少40％的序列一致性，並且逐漸優選地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白與REC8蛋白具有至少45％的序列相似性，並且逐漸優選地，具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述REC8蛋白在GenBank資料庫中的序列登錄號為NP_196168，對應於所附序列表中的SEQ ID NO:35。

使用在默認參數下的BLASTP程序，計算spo11-1、spo11-2、prd1、PRD2、DFO、PAIR1或REC8蛋白的在此所提供的蛋白序列一致性和相似性的值。使用評分矩陣BLOSUM62，進行相似性計算。

可通過消除、阻斷或降低其功能，或者有利地通過阻止或下調相應基因的表達，來獲得對上述SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白的抑制。在申請WO2010/00431中詳細公開了SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白以及所述蛋白的抑制。

例如，所述蛋白的抑制可以通過誘變相應的基因或其啟動子，並選擇所述蛋白的活性部分或完全喪失的突變體來獲得。所述抑制公開在WO 2010/00431的第5頁的最後一段開始至第6頁的第4段結束，以及第6頁的最後一段，這些內容通過引用併入本文中。

可替代地，通過使相應的基因沉默來獲得目標蛋白的抑制。這種抑制公開在WO2010/00431的第7頁第4段開始至第9頁的倒數第二段結束，以及第10頁的前三段，這些內容通過引用併入本文中。

根據本發明的用於獲得能夠產生未減數分裂配子的植物的方法的優選實施方式，所述方法包括：

a)提供在TDM基因的等位基因內具有顯性突變，從而具有產生SDR 2n配子的能力的植物，該植物對於該突變是雜合的；

b)提供在選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因內具有突變，從而抑制由所述等位基因編碼的蛋白的植物，所述植物對於該突變是雜合的；

c)提供在REC8基因的等位基因內具有突變，從而抑制由所述等位基因編碼的蛋白的植物，所述植物對於該突變是雜合的；

e)使步驟a)、步驟b)和步驟c)的植物雜交，以獲得在TDM基因的等位基因內具有顯性突變，在選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因內具有突變，以及在REC8基因的等位基因內具有突變的植物，所述植物對於每一突變都是雜合的；

f)使步驟e)的植物自花授粉，以獲得對於TDM基因內的突變是純合的，對於選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因內的突變是純合的，以及對於REC8基因內的突變的是純合的植物。

根據本發明的用於獲得能夠產生未減數分裂配子的植物的方法的另一優選實施方式，所述植物是轉基因植物，並且所述方法包括：

a)用含有本發明的包含如上定義的TDM基因中的顯性突變的DNA構建體的載體，含有靶向選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA構建體的載體，以及含有靶向REC8基因的DNA構建體的載體轉化至少一個植物細胞；

b)培養所述轉化的植物細胞，以使在它的基因組中具有含所述DNA構建體的轉基因的植物再生。

在TDM基因中包括顯性突變的DNA構建體的表達，為所述轉基因植物提供了產生2n SDR配子的能力。包括TDM基因中的顯性突變的DNA構建體基因，靶向選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA構建體，以及靶向REC8基因的DNA構建體的共表達，使由這三種基因編碼的蛋白下調，並且為所述轉基因植物提供產生未減數分裂配子的能力。

本發明還涵蓋能夠通過本發明的方法獲得的能夠產生SDR 2n配子或未減數分裂配子的植物。

這特別包括如上定義的在TDM基因內包含顯性突變的植物，以及進一步包括第一突變和第二突變的植物，所述第一突變在選自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因內，其中由所述基因編碼的SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1蛋白因該突變而被抑制，所述第二突變在REC8基因內，其中REC8蛋白因該突變而被抑制。

這還包括由本發明的至少一種DNA構建體遺傳轉化的植物。優選地，所述植物是轉基因植物，其中所述構建體被包含在整合到植物基因組中的轉基因中，從而使它被傳遞給連續的植物後代中。

本發明還涵蓋用於產生SDR 2n配子的方法，其中所述方法包括培養能夠通過本發明的方法獲得的植物並回收由所述植物產生的配子。優選地，所述配子包括至少10％、更優選至少20％，並且逐漸優選地，至少30％、40％、50％、60％、70％、80％或90％的可育2n配子。

本發明還還涵蓋用於產生未減數分裂配子的方法，其中所述方法包括培養能夠通過本發明的方法獲得的植物並回收由所述植物產生的配子。優選地，所述配子包括至少10％、更優選至少20％，並且逐漸優選地，至少30％、40％、50％、60％、70％、80％或90％的可育未減數分裂配子。

本發明適用於農藝學上感興趣的範圍廣泛的單子葉植物或雙子葉植物。作為非限制性實例，可提及的是土豆、稻、小麥、玉米、番茄、黃瓜、苜蓿、甘蔗、甘薯、木薯、三葉草、大豆、射線草、香蕉、甜瓜、西瓜、棉，或觀賞植物(諸如玫瑰、百合、鬱金香和水仙)。

除非另有說明，否則本發明的實施將採用本領域技術範圍內的常規技術。這樣的技術在文獻中有充分的解釋。

附圖說明

除了上述設置之外，本發明還包括其他設置，這些其他設置，將在下文中的描述中顯現出來，並且參照所附附圖，指本發明主題的示例性實施方式，在附圖中：

圖1示出了來自多種被子植物物種的TDM蛋白的比對。使用具有默認參數的T-Coffee(v6.85)對序列進行比對(http://toolkit.tuebingen.mpg.de/t_coffee)。序列比對用BioEdit編輯。僅示出了在TDM蛋白中保守的序列的前半部分。在比對的TDM蛋白中顯示出80％以上一致性的殘基用陰影表示。包括TPP/Q模體的保守區則加邊框來表示。

圖2示出了來自多種被子植物的TDM蛋白、來自十字花目的TDM_樣1蛋白以及來自擬南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodium distachyon)的TDM樣蛋白的系統樹。該分析在Phylogeny.fr平臺上進行並包括以下步驟。使用以下成對比對方法，利用T-Coffee(v6.85)來進行序列比對：10次最佳局部比對(Lalign_pair)，精確的全局比對(slow_pair)。在比對之後，從比對中去除具有缺口的位置。利用在PhyML程序(v3.0aLRT)中實施的最大似然法來重建系統樹。示出了所有物種中的TDM進化枝的蛋白(包括在十字花目中的TDM-樣1)。僅示出了擬南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodium distachyon)的更遠的TDM旁系同源。

At：擬南芥Arabidopsis thaliana，A1：深山南芥Arabidopsis lyrata，Bra：芸苔Brassica rapa，Sly：番茄Solanum lycopersicum，St：馬鈴薯Solanum tuberosum，Csa：黃瓜Cucumis sativus，Eucgr：桉樹Eucalyptus grandis，Cp：番木瓜Carica papaya，ME：木薯Manihot esculenta，TC：可可樹Theobroma cacao，Goraii：雷蒙德氏棉Gossypium raimondii，FV：野草莓Fragaria vesca，Pp：柿樹Prunus persica，LJ：蓮藕Lotus japonicus，MT：蒺藜苜蓿Medicago truncatula，GM：大豆Glycine max，Pv：芸豆Phaseolus vulgaris，VV：菜豆Phaseolus vulgaris，Aq：藍花耬鬥菜Aquilegia caerula，OS：粳稻Oryza sativa japonica，OSINDICA：秈稻Oryza sativa indica，BD二穗短柄草Brachypodium distachyon，SB：高粱Sorghum bicolor，ZM：玉米Zea mays，Si：粟Setaria italica。

圖3顯示spo11-1 rec8(s)-40突變體產生二分體並且是四倍體的。(A～C)。用甲苯胺藍染色的雄性減數分裂產物。(A)野生型產生孢子的四分體。(B)spo11-1 rec8產生孢子的不平衡的多分體。(C)spo11-1 rec8-40產生孢子的二分體。(D～F)有絲分裂的核型。(D)野生型是二倍體，具有排列在有絲分裂中期板上的10個染色體。(E)spo11-1 rec8是二倍體。(F)spo11-1 rec8(s)-40是四倍體，具有排列在在有絲分裂中期板上的20個染色體。比例尺＝10μM。

圖4示出了TDM-P17L、TDM-T16A和TDM-Δ14-19的減數分裂產物。(A)用TDM-P17L轉化的spo11-1 rec8突變體。由(B)TDM-P17L、(C)TDM-T16A或(D)TDM-Δ14-19轉化的野生型植物。與野生型中的四分體(圖3A)和spo11-1 rec8中的多分體(圖3B)相比，觀察到孢子的二分體。

圖5示出了在野生型、spo11-1 rec8、TDM-P17和spo11-1 rec8 TDM-P17植物中的減數分裂的染色體分散。(A至D)野生型的減數分裂。(A)五個二價體在中期I時對齊，和(B)成對的同源染色在末期I時分布至兩個核中。(C)五對姐妹染色單體在兩個中期板上對齊。(D)在末期II，形成四個平衡的核。(E至H)spo11-1 rec8中的減數分裂。第一次分裂類似於有絲分裂，其中，(E)10對染色單體對齊在中期板上，並且(F)分離成兩組10個染色單體。(G)在中期II，單個染色單體未能適當地對齊，導致(H)末期II時產生可變數目的不平衡的核。(I至J)用TDM-P17轉化的野生型植物中的減數分裂。觀察到單次減數第一次分裂樣的分裂。(K至L)用TDM-P17轉化的spo11-1 rec8植物中的減數分裂。觀察到單次有絲分裂樣的分裂。

具體實施方式

實施例

實驗步驟

1.生長條件和基因分型

在21℃下，在16小時至18小時光周期和70％相對溼度下，如先前所述在溫室中(Vignard et al.,PLoS Genet.,2007,3,1894-1906)或者如前所述離體在擬南芥Arabidopsis培養基上(Estelle and Somerville,Mol.Genet.,1987,206,200-206)上培養Arabidopsis擬南芥植物。

如先前所述(d』Erfurth et al.,PLoS Biol.,2009,7,e1000124)對spo11-1-3rec8-2植物進行基因分型。如Cromer et al.,PLoS Genet.,2012,8,e1002865所述對tdm-3植物進行基因分型。

2.EMS誘變和突變鑑定

如先前所述(Crismani et al.,Science,2012,336,1588-1590)進行EMS誘變。通過HigSeqTM2000(Illumina)進行全基因組測序。與擬南芥Arabidopsis thaliana TAIR10(哥倫比亞栽培品種)的對照基因組相比，生成SNP列表。

3.細胞學和倍性分析

使用由d'Erfurth等人(PLoS Genet.,2008,4,e1000274)描述的技術，對雄性減數分裂產物進行觀察、染色體分散和倍性測量。

4.定向誘變構建體和植物轉化

使用TDM U(5'-GACATCGGCACTTGCTTAGAG-3'；SEQ ID NO：36)和TDM L(5'-GCGATATAGCTCCCACTGGTT-3'；SEQ ID NO：37)通過PCR擴增TDM基因組片段。擴增覆蓋ATG前的986個核苷酸和終止密碼子後的537個核苷酸。通過GatewayTM技術(Invitrogen)，將PCR產物克隆到pDONR207TM載體(Invitrogen)中，以產生pENTR-TDM；使用Stratagene QuickChangeTM定點誘變試劑盒，根據製造商的說明對pENTR-TDM進行定向誘變。用於產生TDM的突變形式的誘變引物為SEQ ID NO：38至SEQ ID NO：41：

-TDM-P17L:5′-GAGTTTACTATACTCTGCCGCCGGCGAGAAC-3′；

-TDM-T16A:5′-CTCCACCTGGAGTTTACTATGCCCCGCCGCCGGCGAGA-3′；

-TDM-Y14A:5』-CCACCTGGAGTTGCGTATACTCCGCCGCGGCG-3′；

-TDM-Δ14-19:5』-CCACCTGGAGTTGCGAGAACAAGTGATCATGTGGC-3』；

以及它們各自的反向互補引物。為了產生用於植物轉化的雙元載體，使用Gateway TM系統的二元載體pGWB1進行LR重組反應(Nakagawa et al.,Journal of Bioscience and Bioengineering.2007,104,34-41)。對野生型植物以及分離成spo11-1或rec8或tdm-3突變的植物群，使用農桿菌介導的花浸漬法(Clough,S.J.and Bent A.F.,Plant J.,1998,16,735-743)轉化得到的二元載體pTDM，pTDM-P17L，pT16A和pTDM-Y14A。在含有20mg/L潮黴素的瓊脂板上對轉化的植物進行選擇。

實施例1：TDM中的顯性突變導致提早退出減數分裂

為了鑑定控制減數分裂進程的新基因，基於以下觀點來設計遺傳篩選：導致遺漏第二次減數分裂的突變，諸如osd1和cyca1；2/tam將恢復僅在第二次減數分裂中具有不平衡的染色體分離缺陷的突變體的可育力(d』Erfurth et al.,PLoS Biol.,2009,7,e1000124；d』Erfurth et al.,PLoS Genet.,2010,6,e1000989和WO 2010/079432)。這是spo11rec8雙突變體的情況，其中第一次減數分裂類似於有絲分裂(姐妹染色單體平衡分離至相對極)，但是第二次分裂是不平衡的且產生非整倍體的配子，因此具有非常有限的可育力(圖5)(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。在OSD1(d』Erfurth et al.,PLoS Biol.,2009,7,e1000124 2009)或CYCA1；2/TAM(d』Erfurth et al.,PLoS Genet.,2010,6,e1000989)中的導致在減數第二次分裂前退出減數分裂的突變，確實能夠恢復spo11-1 rec8的可育力。因此，基於spo11-1 rec8的可育力的恢復來運行遺傳篩選，旨在鑑定出賦予比osd1或tam還相似缺陷的突變體。儘管它們具有減數分裂分離的缺陷，spo11-1 rec8植物產生了經甲基磺酸乙酯(EMS)誘變的足夠的剩餘種子。假定對EMS突變是雜合的M1植物進行自花授粉，並且在5個批次中收穫以產生M2家族。篩選了與spo11-1 rec8非誘變對照相比，可育力增加的約2000個M2家族(400個批次)。

三個批次分離具有增加可育力的植物。基因分型證實，它們是spo11-1 rec8突變體，這表明了其是真正的抑制因子。通過甲苯胺藍染色對雄性減數分裂產物進行的分析示出，在所有三種情況下，可育植物產生孢子的二分體(如在osd1或cyca1；2/tam中所觀察到的)，而不是四分體(如在野生型中所觀察到的)，表明了在這些植物中並沒有發生第二次減數分裂(圖3)。候選基因(CYCA1；2/TAM和OSD1)的測序確定了CYCA1；2/TAM在三個家族中的兩個中的隱性突變。所確定的突變是外顯子7(TAIR10chr1：29082522C>T)中的剪接位點，和5'UTR區域中的引入上遊框外起始密碼子(TAIR10chr1：29084174G>A)的突變。互補試驗表明它們是等位基因，證實了CYCA1；2中的突變引起二分體表型和可育力的恢復。第三個家族(spo11rec8(s)-40)在OSD1和CYCA1；2中沒有突變，是本研究的重點。

出乎意料地，染色體分散表明四種植物是四倍體的(圖3)。這表明因果突變(causal mutation)是顯性的，並且導致在M1植物的雄性和雌性器官中均產生二倍體配子。與野生型相比，以～100X覆蓋度對兩種姐妹植物的大部分所進行全基因組測序顯示出1144個SNP的存在。然而，僅15個SNP呈現為純合子。這些少數純合子SNP分散在基因組中，表明它們在誘變前就存在於spo11-1rec8株系中，而不是由於EMS誘導的突變所固定的。幾乎所有檢測到的突變都是雜合子這一事實進一步表明因果突變是顯性的。該突變會在M1植物中顯型表達，在與spo11-1 rec8突變組合下，導致二倍體克隆配子的產生(如在spo11-1rec8osd1三重突變體中觀察到的)(MiMe,d』Erfurth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432)，因此四倍體M2植物中維持了來自M1植物的EMS誘導的突變的雜合性。

然後，在雜合子SNP中尋找候選的因果突變。在這1129個突變中，預測341個突變將影響編碼序列(無義、錯義或剪接位點)。其中，TDM中導致胺基酸變化的突變(TDM-P17L)由於潛在的因果顯性突變而成為良好候選者。之前已證實，TDM對於減數第二次分裂結束時退出減數分裂是必需的。即使在tdm敲除突變體中觀察到的減數分裂缺陷(額外的一輪分裂)與(spo11rec8(s)-40)缺陷顯著不同，TDM中的顯性突變仍然是(spo11rec8(s)-40)中的因果突變的潛在候選者。

為了驗證該假設，產生含有TDM基因(包括啟動子和終止子)的基因組克隆，並使其突變以重新產生在篩選中所鑑定的突變(TDM-P17L)，其中TDM基因能夠補充tdm-3突變體(n＝8個轉化體，100％四分體)。當引入spo11-1rec8植物中時，TDM-P17L克隆恢復了初代轉化體的可育力(n＝2/3，spo11-1 rec8：0.1個種子/果實(n＝197)，spo11-1 rec8 TDM-P17L#15：25個種子/果實(n＝15)，spo11-1 rec8 TDM-P17L#67：48個種子/果實(n＝10))，並產生二分體(圖4，表II)。這表明TDM中的突變確實是spo11rec8(s)-40中的因果顯性突變。對spo11-1rec8TDM-P17L轉化體中減數分裂染色體分散的分析顯示出，有絲分裂樣的第一次分裂，其中10個單價體在中期I時對齊，並且姊妹染色單體在後期I分離，且不進行第二次分裂(圖5)。接下來，研究了spo11-1rec8 TDM-P17L後代的倍性水平。在自交子代中，僅發現四倍體(4n)(表III)。當spo11-1rec8 TDM-P17L花粉用於使野生型植物受精時，所有得到的子代都是三倍體(表III)。當spo11-1 rec8 TDM-P17L胚珠經野生型花粉粒受精時，僅發現三倍體植物(表III)。這證實了被TDM-P17L轉化的spo11-1 rec8突變體產生高水平的雄性和雌性(100％)有絲分裂樣來源的孢子，其導致功能性二倍體配子。

當引入到野生型植物和tdm-3突變體中時，TDM-P17L基因組克隆通過產生二分體來修飾兩種基因型的減數分裂表型(圖4，表II)。

表II：初代轉化體的減數分裂產物

產生二分體的TDM-P17L植物顯示出野生型第一次分裂並且不發生減數第二次分裂(圖5)，從而形成2n配子，以及來自osd1和cyca1；2/tam之一的表型重現(d』Erfurth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432；d』Erfurth et al.,PLoS Genet.,2010,6,e1000989)。在TDM-P17L植物的後代中測量倍性水平(表III)。在自交子代中，發現了四倍體和三倍體。當TDM-P17L胚珠經野生型花粉粒受精時，分離出二倍體植物和三倍體植物(表III)。

表III：spo11-1 rec8 TDM-P17L和TDM-P17L後代的倍性

總之，tdm-p17L顯性突變賦予了與隱性osd1或tam突變類似的減數分裂缺陷，使在第二次分裂之前提前退出減數分裂，從而產生二倍體的雄性和雌性配子。

TDM屬於植物中保守的小家族蛋白。例如，擬南芥Arabidopsis基因組含有與TDM顯示出顯著序列相似性的5個其他基因(圖2)。這些類TDM基因的功能是未知的。蛋白序列的分析表明，因果突變在僅TDM蛋白亞家族中保守的小結構域中，所述TDM蛋白亞家族通常每個植物物種含有一個或兩個基因(圖1)。Pro17胺基酸以及相鄰的Thr16胺基酸是絕對保守的(圖1)。這就限定了T16上的最小共有磷酸化位點。為了對此進行驗證，通過用不可磷酸化的胺基酸(TDM-T16A)來置換可磷酸化的胺基酸，並通過刪除整個保守結構域(TDM-Δ14_19)來建立基因組TDM基因的兩個其它潛在的磷酸化丟失形式。當引入到野生型植物中時，TDM-T16A和TDM-Δ14_19都以顯性方式產生了二分體表型，重現了TDM-P17L的作用(表II；圖4)。此外，當引入到tdm-3突變體時，TDM-Δ14_19也顯示二分體表型(表II)。然而，當引入到野生型時，TDM酪氨酸14(TDM-Y14A)(結構域的保守性稍低的胺基酸)的突變不能產生二分體表型，但能夠補充tdm-3突變(表II)。總之，TDM-P17L、TDM-T16A和TDM-Δ14-19突變的表達同樣能夠顯性地提早退出減數分裂。由於TP是潛在的磷酸化位點，這個結果表明TDM可能受到磷酸化的調節以確保減數第一次分裂至減數第二次分裂的過渡。

序列表

國家農藝研究所

在植物中產生二倍體配子的TDM基因中的顯性突變

539EP166

PatentIn 3.5版

434

PRT

擬南芥（Arabidopsis thaliana）

Met Cys Pro Cys Val Glu Arg Arg Ala Pro Pro Gly Val Tyr Tyr Thr

1 5 10 15

Pro Pro Pro Ala Arg Thr Ser Asp Asp Val Ala Ala Met Pro Met Thr

20 25 30

Glu Arg Arg Arg Pro Pro Tyr Ser Cys Ser Ser Ser Ser Glu Arg Arg

35 40 45

Asp Pro Phe His Ile Val His Lys Val Pro Ser Gly Asp Ser Pro Tyr

50 55 60

Val Arg Ala Lys His Ala Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala

65 70 75 80

Ile Ser Leu Phe Trp Thr Ala Ile Asn Ala Gly Asp Arg Val Asp Ser

85 90 95

Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Gly Arg Ser Asp

100 105 110

Glu Gly Ile Glu Ala Ile Lys Ser Phe Arg Tyr Leu Cys Ser Phe Glu

115 120 125

Ser Gln Asp Ser Ile Asp Asn Leu Leu Leu Glu Leu Tyr Lys Lys Ser

130 135 140

Gly Arg Ile Glu Glu Glu Ala Val Leu Leu Glu His Lys Leu Gln Thr

145 150 155 160

Leu Glu Gln Gly Met Gly Phe Gly Gly Arg Val Ser Arg Ala Lys Arg

165 170 175

Val Gln Gly Lys His Val Ile Met Thr Ile Glu Gln Glu Lys Ala Arg

180 185 190

Ile Leu Gly Asn Leu Gly Trp Val His Leu Gln Leu His Asn Tyr Gly

195 200 205

Ile Ala Glu Gln His Tyr Arg Arg Ala Leu Gly Leu Glu Arg Asp Lys

210 215 220

Asn Lys Leu Cys Asn Leu Ala Ile Cys Leu Met Arg Met Ser Arg Ile

225 230 235 240

Pro Glu Ala Lys Ser Leu Leu Asp Asp Val Arg Asp Ser Pro Ala Glu

245 250 255

Ser Glu Cys Gly Asp Glu Pro Phe Ala Lys Ser Tyr Asp Arg Ala Val

260 265 270

Glu Met Leu Ala Glu Ile Glu Ser Lys Lys Pro Glu Ala Asp Leu Ser

275 280 285

Glu Lys Phe Tyr Ala Gly Cys Ser Phe Val Asn Arg Met Lys Glu Asn

290 295 300

Ile Ala Pro Gly Thr Ala Asn Lys Asn Tyr Ser Asp Val Ser Ser Ser

305 310 315 320

Pro Ala Ser Val Arg Pro Asn Ser Ala Gly Leu Tyr Thr Gln Pro Arg

325 330 335

Arg Cys Arg Leu Phe Glu Glu Glu Thr Arg Gly Ala Ala Arg Lys Leu

340 345 350

Leu Phe Gly Lys Pro Gln Pro Phe Gly Ser Glu Gln Met Lys Ile Leu

355 360 365

Glu Arg Gly Glu Glu Glu Pro Met Lys Arg Lys Lys Leu Asp Gln Asn

370 375 380

Met Ile Gln Tyr Leu His Glu Phe Val Lys Asp Thr Ala Asp Gly Pro

385 390 395 400

Lys Ser Glu Ser Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Glu Glu

405 410 415

Ala Glu Glu Glu Glu Glu Glu Arg Leu Gln Gly Glu Leu Lys Thr Ala

420 425 430

Glu Met

426

PRT

深山南芥（Arabidopsis lyrata）

Met Cys Pro Cys Glu Glu Arg Arg Ala Pro Pro Gly Val Tyr Tyr Thr

1 5 10 15

Pro Pro Pro Ala Arg Thr Ser Tyr Val Ala Ala Met Pro Leu Ser Glu

20 25 30

Arg Arg Arg Pro Pro Phe Ser Cys Ser Ser Ser Ser Glu Lys Arg Asp

35 40 45

Pro Phe His Ile Val His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val

50 55 60

Arg Ala Lys His Ala Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala Ile

65 70 75 80

Ser Leu Phe Trp Thr Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala

85 90 95

Leu Lys Asp Met Val Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu

100 105 110

Gly Ile Glu Ala Ile Arg Ser Phe Arg Tyr Leu Cys Ser Phe Glu Ser

115 120 125

Gln Asp Ser Ile Asp Asn Leu Leu Leu Glu Leu Tyr Lys Lys Ser Gly

130 135 140

Arg Ile Glu Glu Glu Ala Glu Leu Leu Glu His Lys Leu Lys Thr Leu

145 150 155 160

Glu Gln Gly Met Gly Phe Gly Gly Arg Val Ile Arg Ala Lys Arg Val

165 170 175

Gln Gly Lys His Val Thr Met Thr Ile Glu Gln Glu Lys Ala Arg Val

180 185 190

Leu Gly Asn Leu Gly Trp Val His Leu Gln Leu His Asn Tyr Gly Ile

195 200 205

Ala Glu Gln His Tyr Arg Arg Ala Leu Gly Leu Glu Arg Asp Lys Asn

210 215 220

Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Arg Met Gly Arg Ile Pro

225 230 235 240

Glu Ala Lys Ser Leu Leu Asp Asp Val Arg Asp Ser Pro Thr Glu Ser

245 250 255

Glu Cys Gly Asp Glu Pro Phe Ala Lys Ser Tyr Asp Arg Ala Val Glu

260 265 270

Met Leu Ala Glu Ile Glu Ser Lys Asn Pro Glu Ala Asp Leu Ser Asp

275 280 285

Lys Phe Tyr Ala Gly Cys Ser Phe Ala Asn Gly Met Lys Glu Asn Ile

290 295 300

Ala Pro Gly Ile Ala Asn Lys Asn Tyr Ser His Val Ser Ser Ser Pro

305 310 315 320

Ala Ser Val Val Pro Asn Ser Ala Gly Leu Tyr Thr Gln Pro Arg Gly

325 330 335

Cys Arg Ala Gly Met Tyr Glu Glu Glu Thr Arg Gly Ala Ala Arg Lys

340 345 350

Leu Leu Phe Glu Lys Pro Lys Pro Phe Ala Ser Glu Gln Ile Lys Ile

355 360 365

Leu Lys Arg Gly Glu Glu Glu Pro Gln Lys Arg Lys Lys Leu Asp Pro

370 375 380

Asn Met Ile Gln Tyr Leu His Glu Phe Ile Lys Asp Thr Ala Asp Gly

385 390 395 400

Pro Lys Asn Glu Ser Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Glu

405 410 415

Glu Glu Glu Glu Arg Leu Gln Ala Glu Thr

420 425

432

PRT

芸苔（Brassica rapa）

Met Cys Pro Phe Glu Asp Arg Arg Ala Pro Pro Gly Val Tyr Trp Thr

1 5 10 15

Pro Pro Pro Ala Arg Arg Thr Glu Asn Ala Ala Ala Met Pro Met Pro

20 25 30

Leu Ser Glu Arg Arg Arg Pro Pro Ser Ser Glu Lys Arg Asp Pro Phe

35 40 45

His Ile Val His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala

50 55 60

Lys His Ala Gln Leu Val Tyr Lys Asp Pro Asn Arg Ala Ile Ser Leu

65 70 75 80

Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys

85 90 95

Asp Met Ala Val Val Phe Lys Lys Leu Asp Arg Ser Asp Glu Gly Ile

100 105 110

Glu Ala Ile Lys Ser Phe Arg Tyr Leu Cys Pro Phe Glu Ala Gln Asp

115 120 125

Ser Ile Asp Asn Leu Leu Leu Glu Leu Tyr Lys Lys Ser Gly Arg Ile

130 135 140

Gln Glu Glu Ala Glu Leu Leu Glu His Lys Leu Lys Val Ile Glu Gln

145 150 155 160

Gly Met Gly Phe Gly Gly Arg Ile Val Arg Ala Lys Arg Val Gln Gly

165 170 175

Lys His Val Thr Met Thr Val Glu Gln Glu Lys Ala Arg Val Leu Gly

180 185 190

Asn Leu Gly Trp Val His Leu Gln Leu His Asn Tyr Gly Ile Ala Glu

195 200 205

Gln His Tyr Arg Arg Ala Leu Cys Leu Glu Pro Asp Lys Asn Lys Gln

210 215 220

Cys Asn Leu Ala Ile Cys Leu Met Arg Met Gly Arg Ile Pro Glu Ala

225 230 235 240

Lys Ser Met Ile Asp Ala Val Arg Asp Ser Ser Ala Glu Thr Glu Ser

245 250 255

Gly Asp Glu Pro Phe Thr Lys Ser Tyr Asn Arg Ala Val Glu Met Leu

260 265 270

Ala Glu Val Glu Ser Lys Asp Pro Glu Asp Gly Leu Ser Asp Lys Phe

275 280 285

Tyr Ala Gly Cys Ser Phe Ala Asn Gly Thr Met Lys Glu Asn Lys Ala

290 295 300

Pro Arg Asn Ala Asn Arg Asn His Ser His Val Pro Pro Ser Pro Ala

305 310 315 320

Ser Val Arg Gln Asn Ser Ala Gly Leu Phe Thr Gln Pro Arg Gly Cys

325 330 335

Lys Gly Asp Pro Lys Asn Gly Val Ser Glu Glu Glu Thr Gly Gly Ala

340 345 350

Ala Arg Lys Leu Leu Phe Asp Lys Pro Ile Gly Ser Gln Arg Val Lys

355 360 365

Leu Leu Lys Ser Gly Glu Gly Glu Gln His Val Lys Gly Lys Lys Leu

370 375 380

Asp Gln Asn Met Ile Gln Asp Leu His Glu Tyr Ile Lys Asp Thr Ala

385 390 395 400

Asp Cys Leu Lys Ser Gly Ser Lys Lys Ser Trp Ala Asp Met Ala Glu

405 410 415

Glu Glu Asp Glu Glu Ser Val Gln Ser Gln Leu Lys Thr Ala Lys Ile

420 425 430

670

PRT

番木瓜（Carica papaya）

Met Trp Ala Asn Asp Arg Lys Pro Leu Gly Arg Gly Phe Ser Thr Pro

1 5 10 15

Gln Pro Thr Trp Asn Ser Arg Pro Arg Asp Ser Leu Val Met Pro Met

20 25 30

Ser Glu Arg Lys Ser Ser Ser Pro Val His Lys Gly Asp Leu Phe His

35 40 45

Val Val His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys

50 55 60

His Val Gln Leu Ile Glu Lys Asp Pro Ser Arg Ala Ile Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Ile Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Asp Ala Gln Glu Ser

115 120 125

Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu

130 135 140

Glu Glu Val Glu Leu Leu Gln His Lys Leu Lys Asn Ile Glu Gly Val

145 150 155 160

Thr Phe Gly Gly Lys Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys

165 170 175

Ile Gln Ile Thr Val Glu Gln Glu Lys Ser Arg Ile Leu Gly Asn Leu

180 185 190

Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ser Ala Glu Gln Tyr

195 200 205

Tyr Arg Lys Ala Leu Ser Leu Glu Val Asp Lys Asn Lys Gln Cys Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Ala Glu Ala Lys Ser

225 230 235 240

Leu Leu Leu Ala Val Arg Asp Ser Pro Lys Lys Arg Gln Met Asp Glu

245 250 255

Ser Cys Ala Lys Ser Phe Glu Arg Ala Tyr Gln Ile Leu Arg Glu Lys

260 265 270

Glu Ser Gln His Glu Arg Ser Gly Arg Ser Glu Asp Ser Phe Tyr Val

275 280 285

Val Asp Asn Glu Ser Ser Asn Ser Asp Lys Asn Gly Leu Asp Asn Trp

290 295 300

Ser Asn Gly Cys Cys Ser Glu Ser Ser Leu Glu Met Ser Asn Phe Ala

305 310 315 320

Asp Gly Met Lys Arg Tyr Gln Asn Gly Lys Glu Thr Glu Ala Ser Leu

325 330 335

Gly Asn Asn Ser Tyr Ser Thr Cys Thr Arg Arg Lys Ser Gly Ile Phe

340 345 350

Leu Thr Gln Pro Arg Arg Cys Ser Trp Gly Leu Glu Asp Glu Asn Tyr

355 360 365

Arg Glu Ile Trp Gly Lys Ser Leu Gly Gly Ser Ser Val Arg Lys Leu

370 375 380

Ser Phe Glu Gln Asn Lys Ile Thr Glu Asn Ala Phe Ser His Ala Ser

385 390 395 400

Gly Asn Leu Lys Ser Gly Ile Leu Leu Thr Gln Pro Arg Arg Cys Ser

405 410 415

Trp Gly Leu Lys Asp Glu Lys Tyr Arg Glu Arg Leu Gly Lys Ser Leu

420 425 430

Gly Gly Ser Ser Val Arg Lys Leu Ser Phe Glu Gln Ala Lys Ile Thr

435 440 445

Glu Asn Ala Phe Ser His Ala Ser Gly Asn Leu Ile Glu Glu Pro Val

450 455 460

Thr Cys Thr Asp Asp Asn Val Glu Ser His Ser Gly Val Leu Leu Thr

465 470 475 480

Gln Pro Arg Val Ser Leu Gly Ile Asn Glu Asp Gln Arg Gly Lys Trp

485 490 495

Gly Glu Asn Met Val Asp Ser Ser Pro Arg Gln Leu Ser Phe Glu Tyr

500 505 510

Lys Asn Gln Lys Glu Asp Pro Val Asp Glu Asn Pro Glu Asn Leu Lys

515 520 525

Asp Lys Ala Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Asp Glu Glu

530 535 540

Met Tyr Lys Glu Leu Val Cys Gly Asp Thr Pro Ser Lys Tyr Phe Asp

545 550 555 560

Gly Leu Lys Ser Lys Glu Glu Glu Glu Lys Phe Ser Asp Glu Asn Leu

565 570 575

Asp Thr Asn Leu Met Tyr Gln Ser Pro Tyr Tyr Pro Leu Ser Gln Thr

580 585 590

Glu Ala Ala Gly Gln Glu Leu Lys Ser Phe Tyr Gln Lys Asp Gly Tyr

595 600 605

Asn Gly Thr Ser Gly Asn Ala Val Ser Ser Arg Asn Pro Thr Ala Arg

610 615 620

Arg Ser Leu Tyr Phe Gly Gly Asp Ser Asn Ser Leu Pro Leu Lys Glu

625 630 635 640

Lys Asp Ser Phe Thr Gly Lys Asn Ile Ser Met Thr Arg Arg Asn Arg

645 650 655

Leu Lys Val Phe Thr Glu Ile Thr Leu Leu Pro Asp Ser Pro

660 665 670

676

PRT

可可樹（Theobroma cacao）

Met Trp Ser Asn Glu Lys Asn Leu Pro Ser Arg Ser Arg Gly Phe Phe

1 5 10 15

Thr Pro Gln Pro Pro Ala Trp Lys Lys Ala Gln Ser Ser Val Leu Pro

20 25 30

Met Ser Glu Arg Lys Arg Ile Ser Pro Ala Asp Gln Ala Asp Ser Phe

35 40 45

His Val Ile His Lys Val Pro Ala Ser Asp Ser Pro Tyr Gly Arg Ala

50 55 60

Lys His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu

65 70 75 80

Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys

85 90 95

Asp Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile

100 105 110

Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu

115 120 125

Ser Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val

130 135 140

Glu Glu Glu Ile Glu Met Leu Gln Asn Lys Leu Arg Asn Ile Glu Gly

145 150 155 160

Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly

165 170 175

Lys Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly

180 185 190

Asn Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu

195 200 205

Gln His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Met Asn Lys Gln

210 215 220

Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Ser Glu Ala

225 230 235 240

Lys Ser Leu Leu Gln Asp Val Lys Ala Ser Ser Gly Asn Glu Gln Met

245 250 255

Asp Glu Ser His Ser Lys Ser Tyr Glu Arg Ala Leu Asp Met Leu Ile

260 265 270

Gln Val Glu Ser Gln Ser Met Leu Glu Pro Val Ala Lys Glu Pro Asp

275 280 285

Lys Gly Arg Glu Ile Gln Arg Pro Ser Thr Pro Cys Arg Asp Arg Gly

290 295 300

Leu Lys Glu Ala Gly Ile Phe Leu Pro Arg Asn Glu Asp Asn Ile Ser

305 310 315 320

Gly Phe Met Gly Arg Arg Arg Leu Pro Tyr Ala His Trp Glu Gly Lys

325 330 335

Met Leu Ile Asp Glu Gln Asn Gly Glu Ser Tyr Arg Arg Asn Pro Leu

340 345 350

Glu Lys Asn Asp Asn Phe Pro Gly Tyr Asp Asp Arg Ser Ser Lys Cys

355 360 365

Thr Pro Ile Gly Gln Lys Gly Tyr Leu Gln Ser Ser Pro Gln Ser Met

370 375 380

Phe Thr Glu Lys Trp Arg Ile Gly Ser Tyr Trp Glu Ser Pro Cys Glu

385 390 395 400

Gly Tyr Ser Thr Gly Glu Glu Val Gly Ser Ala Gln Lys Lys Ile Tyr

405 410 415

Ala Ser Ser Ala Ala Ser Lys Lys Asn Ser Glu Ala Leu Phe Thr Gln

420 425 430

Pro Arg Arg Cys Ser Gln Gly Phe Asn Asn Ala Asp Gln Lys Arg Gly

435 440 445

Gly Arg Trp Gly Glu Asp Thr Val Arg Asn Ser Ile Arg Lys Leu Ser

450 455 460

Phe Glu Gln Ser Leu Thr Ser Glu Ser Glu Pro Leu His Ser Ile Gln

465 470 475 480

Asn Leu Asn Glu Lys Pro Gln Ala Ser Asn Asn Gly Lys Ser Glu Asn

485 490 495

Ser Ala Thr Gly Pro Val Glu Glu Glu Val Gln Glu Gly Leu Ser Gly

500 505 510

Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Leu Trp Leu Asn Asn Arg

515 520 525

Asp Gln Arg Met Glu Arg Trp Ala Glu Glu Ser Val Gly Cys Pro Phe

530 535 540

Arg Lys Leu Ser Phe Glu Lys Asn Ile Thr Gly Val Thr Pro His Ser

545 550 555 560

Ala Asp Gly Leu Asn Gly Glu Pro Leu Phe Ser Ser Lys Asp Glu Ser

565 570 575

Glu Ile Gly Leu Glu Arg Pro Ala Asn Ala Pro Asn Lys Lys Ser Trp

580 585 590

Ala Asp Met Val Glu Glu Glu Glu Lys Glu Glu Leu Leu Asn Ser Tyr

595 600 605

Asp Gly Phe Asn Arg Glu Glu Val Phe Asn Asp Glu Asn Leu Asn Ser

610 615 620

Asn Ile Ile Tyr Pro Arg Pro Asp Cys Lys Asp His Ile Gly Asn Ile

625 630 635 640

Thr Gln Gln Leu Glu Ser Phe Asp Met Lys Gly Gly Asp Asn Ala Ser

645 650 655

Ala Asn Thr Val Ser Ser Arg Arg Asn Arg Leu Pro Val Phe Arg Asp

660 665 670

Ile Thr Ser Ser

675

631

PRT

木薯（Manihot esculenta）

Met Trp Ser His His Glu Asn Asn Ile Pro Ala Arg Gly Phe Ser Thr

1 5 10 15

Pro Pro Pro Ser Trp Lys Ser Pro His Cys Leu Ala Ala Met Pro Met

20 25 30

Ser Glu Arg Lys Ser Val Ser Ser Gly Cys Lys Arg Asp Leu Phe His

35 40 45

Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys

50 55 60

His Val Gln Leu Ile Glu Lys Asp Pro Ser Arg Ala Ile Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Ile Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg Arg Leu Cys Pro Tyr Asp Ser Gln Glu Ser

115 120 125

Ile Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu

130 135 140

Glu Glu Ile Glu Met Leu His Leu Lys Leu Lys Asn Ile Glu Glu Gly

145 150 155 160

Ile Ala Phe Gly Gly Lys Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys

165 170 175

Lys Ile Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln His His Asp Tyr Gly Leu Ala Glu Gln

195 200 205

Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Pro Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Ala Val Ser Asp Ser Cys Gly Ser Lys Gln Met Asp

245 250 255

Asp Ser Tyr Ala Lys Ser Phe Glu Arg Ala Val Asp Ile Leu Thr Glu

260 265 270

Leu Glu Ser Lys Ser Val Leu Lys Pro Ala Glu Glu Asp Lys Glu Asn

275 280 285

Gln Arg Ser Leu Ala Ser Pro Leu Leu Ser Asp Lys His Asp Arg Gly

290 295 300

Ser Tyr Cys Gly Ile His Ser Glu Glu Gln Gly Asn Phe Ile Ser Ser

305 310 315 320

Ser Gly Lys Gly Pro Gly Leu Ala Asn Lys Arg Met Leu Asp Ser Pro

325 330 335

Ala Ala Val Leu Tyr Thr Gln Pro Lys Arg Val Met Gly Arg Ser Asp

340 345 350

Glu Glu Glu Gln Arg Arg Gly Val Gly Trp Glu Asn Asp Thr Val Glu

355 360 365

Lys Pro Ser Lys Asn Val Ser Ala Cys Ile Ile Arg Ser Leu Asp Gly

370 375 380

Glu Leu Leu Asp Pro Pro Ala Glu Arg Asn Trp Arg Glu Lys Ser Trp

385 390 395 400

Thr Glu Val Ala Gln Gly Lys Ile Thr Gly Val Thr Val Pro Tyr Gln

405 410 415

Phe Ser Gln Pro Arg Ile Arg Ala Phe Thr Gly Tyr Asn Asp Ala His

420 425 430

Leu Lys Asp Glu Asn Val Thr Lys Ser Asp Ser Gln Gln Pro Ser Trp

435 440 445

Arg Ser Asn Ala Arg Glu Thr Gly Gly Gln Met Gly Ser Thr Asn Glu

450 455 460

Lys Ser Asp Ala Ser Ser Lys Phe His Leu Glu Gln Asn Met Val Val

465 470 475 480

Asp Asp Ala Arg Gln Ser Glu Thr Ser Ile Asp Gly Lys Cys Gly Gln

485 490 495

Thr Phe Gly Ile Asn Gly Cys Leu Gly Lys Asn Ser Ser Ser Lys Phe

500 505 510

Ala Ile Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Lys Leu

515 520 525

Leu Thr Gly Lys Asp Leu Ser Pro Tyr Phe Asp Gly Gly Trp Asp Tyr

530 535 540

Glu Glu Glu Ser Ala Asp Glu Asn Gln Asp Ser Asn Ile Ile His Gln

545 550 555 560

Thr Ser Cys Pro Lys Ser Pro Ala Glu Ala Ile Asn Gln Lys Phe Glu

565 570 575

Ala Phe Asp Leu Lys Asp Gly Phe Ala Ala Phe Ser Asn Ala Val Ser

580 585 590

Pro Arg Asn Pro Thr Val Arg Arg Ser Leu Arg Phe Asp Ala Lys Asn

595 600 605

Asp Phe Ser Thr Arg Lys Lys His Arg Leu Gln Val Phe Gln Asp Ile

610 615 620

Thr Pro Ser Ile Asp Ser Pro

625 630

673

PRT

野草莓（Fragaria vesca）

Met Trp Ala Arg Asp Arg Ser Tyr Pro Pro Ala Gly Phe Phe Thr Pro

1 5 10 15

Pro Pro Pro Arg Lys Ser Gly Pro Pro Asn Pro Pro Pro Met Ser Glu

20 25 30

Arg Lys Arg Val Ser Pro Ala Ala Ala Arg Ser Asp Leu Phe His Val

35 40 45

Ala His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln

50 55 60

Val Gln Leu Ile Asp Lys Asn Pro Ser Lys Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ala Glu Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Glu Ser Gln Glu Ser Leu

115 120 125

Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu Asp

130 135 140

Glu Ile Glu Thr Leu Gln Ser Lys Leu Lys Arg Met Asp Glu Gly Ile

145 150 155 160

Ala Phe Asn Gly Lys Arg Thr Lys Thr Ala Arg Ala Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Val Leu Gly Asn Leu

180 185 190

Ala Trp Ala Tyr Met Gln Gln Gly Asn Tyr Thr Thr Ala Glu Glu His

195 200 205

Tyr Lys Asn Ala Leu Ala Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Thr Glu Ala Arg His

225 230 235 240

Leu Leu Gln Ala Val Arg Asp Ser Ala Gly Asn Lys Pro Met Asp Glu

245 250 255

Ser Tyr Ala Lys Ser Phe Glu Arg Ser Phe Glu Met Leu Thr Glu Leu

260 265 270

Glu Gln Gln Ser Val Leu Arg Pro Ile Gln Gln Asn Glu Asn Tyr Cys

275 280 285

Thr Gly Ile Ser Arg Thr Pro Lys Pro Ser Gly Ser Gly Tyr Met Pro

290 295 300

Met Pro Pro Arg Arg Trp Thr Asp Gly Pro Glu Tyr Val Thr Val Ala

305 310 315 320

Asn Glu Arg His Arg Glu Ser Phe Arg Pro Gly Ser Cys Ala Lys Ser

325 330 335

Phe Asp Lys Trp Lys Lys Asp Tyr Asp Leu Lys Asn Thr Gly Asp Ser

340 345 350

Arg Pro Ser Phe Ser Ser Ser Val Lys Gln Asn Gln Asp Gly Met Thr

355 360 365

Val Thr Glu Val Asn Pro His Glu Lys Thr Tyr Val Ser Pro Val Leu

370 375 380

Tyr Thr Gln Pro Arg Arg Pro Ser Trp Gly Phe Asn Asp Gly His Gln

385 390 395 400

Arg Ser Glu Ile Trp Gly Asn Gly Val Gly Ser Ser Asn Lys Lys Leu

405 410 415

Pro Pro Glu Arg Ser Ala Gly Asn Val Arg Ala His Val Val Arg Asn

420 425 430

Leu Asn Ala Asp Leu Leu Ala Ser Thr Pro Arg Glu Ser Asp Ser Ser

435 440 445

Leu Ser Ser Arg Ser His Gly Asp Trp Arg Met Pro Gln Arg Asp Ala

450 455 460

Val Ala Arg Pro Val Leu Gln Pro Val Ser Ser Met Thr Ser Arg Gly

465 470 475 480

Asn Glu Gly Tyr Leu Pro Val Arg Asn Glu Ala Met Ile Asp Arg Ser

485 490 495

Ser Lys Tyr Thr Gly Asn Gly Asp Ile Arg Arg Val Thr Trp Asp Asn

500 505 510

Ala Gly Met Gln Lys Ser Ala Ala Gly Ser Gln Val Val Asp Glu Asn

515 520 525

Ala Lys Ala Leu Leu Leu Cys Ile Asp Glu Gly Asp Ser Gln Ser Ser

530 535 540

Gly Thr Thr Val Leu Gly Ser Met Glu Asn Met Asn Ala Ser Val Glu

545 550 555 560

Glu Asp Cys Leu Gly Asp Asn Ser Ser Gly Thr Leu Asp Asn Val His

565 570 575

Gln Ser Pro Ala Glu Asn Glu Arg Pro Thr Leu His Gln Ser Pro Ala

580 585 590

Glu Thr Glu Lys Pro Thr Pro Asp Phe Trp Lys Tyr Ser Gly Lys Lys

595 600 605

Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Glu Leu Arg Ser Gly

610 615 620

Thr Thr Gly Tyr Phe Asp Ser Trp Asn Thr Gly Asp Glu Phe Asp Asp

625 630 635 640

Glu Asn Arg Asn Pro Asn Ile Met Thr Pro Gln Ser Pro Gln Phe Gln

645 650 655

Thr Gln Met Lys Arg Arg Asn Arg Leu Gln Ser Phe Gln Asp Ile Thr

660 665 670

Asn

601

PRT

大豆（Glycine max）

Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Pro Gln Arg Thr Ser Pro Trp Lys Pro Pro His Ser Arg Ser Ser Ser

20 25 30

Val Pro Phe Ser Glu Arg Lys Met Ser Pro Asn Ser Val Asn Lys Ser

35 40 45

Asp Ile Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala

65 70 75 80

Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Gln Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Val Asp Glu Glu Ile Ala Met Leu Cys His Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile

225 230 235 240

Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln

260 265 270

Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Gln Asn Ala Ala Phe Ser

275 280 285

Met Thr Thr Gln Cys Pro Pro Gln Ser Phe Glu Asn Ser Ile Arg Met

290 295 300

Ser Ser Asp Ser Val Gln Ser Arg Ser Glu Asn Arg Ser Glu Ile Ser

305 310 315 320

Glu Gly Asp Ala Pro His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp

325 330 335

Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro Lys Arg Cys

340 345 350

Ser Trp Gly Phe Asn Asn Gly His Arg Arg Glu Ala Trp Gly Asp Ala

355 360 365

Asn Ser Asp Tyr Lys Pro Thr Phe Gly Thr Pro Pro Asn Asp Lys His

370 375 380

Val Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Phe Ser Ser Pro Ala

385 390 395 400

Asn Gly Asn Gly Lys Ala Ala Thr Leu Glu Asp Pro Ala Ile Leu Lys

405 410 415

His Glu Ala Thr Lys Val Thr Ser Ser Asp Ser Leu His Thr Leu Asn

420 425 430

Thr Glu Ser Ala Thr Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Asp

435 440 445

Asp Ser Ser Tyr Gly Ser Ile Leu Ser Glu Ser His Val Thr Val Val

450 455 460

Asn Gly Val Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro Glu Lys Lys

465 470 475 480

Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe

485 490 495

Phe Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala Glu Val Phe

500 505 510

Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser Pro Trp Pro

515 520 525

Gln Asn Gln Pro Asp Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Asp

530 535 540

Lys Lys Asp Gly His Tyr Ala Ser Glu Ser Ala Ile Leu Ser Arg Asn

545 550 555 560

Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly Glu

565 570 575

Lys Arg Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile Thr

580 585 590

Leu Leu Pro Glu Thr Pro Arg Phe Ala

595 600

602

PRT

大豆（Glycine max）

Met Gln Phe Glu Arg Gly Ser Met Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Pro Gln Pro Pro Ser Ser Trp Lys Pro Pro His Ser Gln Ser Pro Ser

20 25 30

Val Pro Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ile

35 40 45

Asp Leu Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala

65 70 75 80

Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Arg Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Arg Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Ile Asp Glu Glu Ile Ala Met Leu His His Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Ala Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile

225 230 235 240

Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln

260 265 270

Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Ser Gln Asn Ala Ala Phe

275 280 285

Ser Met Thr Thr Met Ser Gln Cys Ser Pro Gln Ser Phe Glu Asn Ser

290 295 300

Ile Arg Lys Ser Ser Asp Arg Val Gln Ser Gly Ser Glu Ser Arg Ser

305 310 315 320

Glu Thr Ser Glu Gly Asp Val Pro His Ala Arg Arg Arg Leu Tyr Gln

325 330 335

Ser Pro Asp Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro

340 345 350

Lys Arg Cys Ser Trp Gly Phe Asn Thr Gly Tyr Arg Arg Glu Ala Trp

355 360 365

Gly Asp Val Asn Ser Asp Tyr Lys Pro Ser Phe Gly Thr Pro Pro Asn

370 375 380

Asp Lys His Ala Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Leu Ser

385 390 395 400

Ser Pro Ala Asn Gly Lys Trp Arg Ala Met Thr Leu Glu Asp Gln Ala

405 410 415

Ile Leu Lys His Glu Ala Thr Thr Ala Thr Ser Ser Asp Ser Leu His

420 425 430

Ala Leu Asn Thr Glu Val Ala Met Glu Phe Thr Glu Lys Glu Lys Ser

435 440 445

Ala Ala Asp Asp Ser Ser Tyr Arg Ser Ile Leu Ser Glu Ser His Val

450 455 460

Thr Val Val Asn Gly Ala Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro

465 470 475 480

Glu Lys Lys Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu

485 490 495

Tyr Asp Phe Leu Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala

500 505 510

Glu Val Phe Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser

515 520 525

Ser Trp Leu Gln Asn Leu Pro Glu Trp Ser Asn Lys Lys Leu Glu Ser

530 535 540

Ser Asp Lys Lys Asp Asp Tyr Ala Ser Glu Ser Thr Ile Leu Ser Arg

545 550 555 560

Asn Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly

565 570 575

Glu Lys Lys Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile

580 585 590

Thr Leu Leu Pro Glu Thr Pro Arg Phe Ala

595 600

603

PRT

蓮藕（Lotus japonicus）

Met Pro Phe Glu Arg Asn Ser Pro Ala Arg Cys Phe Met Thr Pro Pro

1 5 10 15

Pro Pro Arg Leu Ser Ser Trp Arg Ser Thr Pro Ser Arg Ser Pro Ile

20 25 30

Met Met Pro Leu Ser Glu Arg Lys Arg Ser Ser Pro Asn Lys Asp Asp

35 40 45

Pro Tyr His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val

50 55 60

Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala Ile

65 70 75 80

Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala

85 90 95

Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu

100 105 110

Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Pro Asp Ser

115 120 125

Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser Gly

130 135 140

Arg Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Gln Ile

145 150 155 160

Glu Asp Gly Val Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser

165 170 175

Gln Gly Lys Lys Ile His Val Thr Ala Glu Gln Glu Ile Ser Arg Ile

180 185 190

Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys Ala

195 200 205

Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg Asn

210 215 220

Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Thr Asn Arg Ile Thr

225 230 235 240

Glu Ala Lys Phe Leu Leu Gln Ala Val Thr Thr Ala Ser Lys His Arg

245 250 255

Lys Met Asp Asp Ser Cys Ala Lys Ser Phe Glu Arg Ala Ser Gln Met

260 265 270

Leu Met Asp Met Glu Ser Ser Ser Ser Gln Cys Ser Pro Leu Ser Asn

275 280 285

Leu Ser Gly Lys Ser Ser Asp Met Val Gln Ser Arg Thr Gln Asn Trp

290 295 300

Ser Val Thr Ser Glu Gly Glu Val Ser Asn Ala Arg Arg Arg Leu Tyr

305 310 315 320

Glu Ser Pro Glu Pro Ala Arg Arg Asp Leu Lys Val Pro Tyr Thr Asn

325 330 335

Thr Lys Lys Cys Ser Trp Gly Phe Asn Asn Gly Pro Gln Arg Glu Thr

340 345 350

Trp Gly Asp Val His Ser Asp Pro Lys Pro Ser Phe Gly Ile Pro Ser

355 360 365

Lys Gln Asn Asn Leu Ser Leu Pro Ala Asn Gly Lys Trp Asn Ala Arg

370 375 380

Thr Met Val Lys Arg Glu Asp Lys Thr Ala Ile Gly Ser Gly Leu Tyr

385 390 395 400

Thr Tyr Ser Asn Thr Glu Thr Asp Arg Lys Phe Lys Glu Asp Lys Phe

405 410 415

Ala Val Glu Ser Asn Asp Met Val Val Asn Gly Ala Asn Gln Phe Ala

420 425 430

Ser Ser Ile Gly Thr Asp Gln Ser Lys Cys Ser Glu Ile Ser Ile Lys

435 440 445

Leu Cys Cys Tyr Pro Phe Cys Gln Leu Asn Ser Ser Leu Ile Asn Leu

450 455 460

His Glu Leu Ala Ser Gly Ser Ile Lys Pro Val Glu Lys Lys Ser Trp

465 470 475 480

Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Gly His Asp Phe Phe Gly

485 490 495

Gly Tyr Thr Arg Phe Asp Gly Gln Glu Gly Ala Gln Val Phe Asn Lys

500 505 510

Glu Asn Glu Asn Ser Asn Ile Val Phe Gln Arg Pro Trp Pro Leu Ser

515 520 525

Glu Thr Lys Cys Ile Ser Lys Lys Leu Glu Ser Met Asp Leu Lys Asp

530 535 540

Gly His His Gly Ser Gly Thr Val Thr Leu Ser Arg Lys Pro Ala Val

545 550 555 560

Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ala Lys Glu Arg Asp Ser

565 570 575

Ser Leu Ser Glu Glu Lys Lys Pro Pro Arg Asn Asn Arg Leu Gln Val

580 585 590

Phe Lys Asp Ile Ala Leu His Pro Glu Thr Pro

595 600

617

PRT

蒺藜苜蓿（Medicago truncatula）

Met Thr Phe Glu Arg Asn Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Ser Ser Ser Ser Ser Trp Lys Ser Arg Pro Val Arg Ser Pro Thr Val

20 25 30

Pro Phe Ser Glu Arg Lys Lys Ser Pro Ala Ala Ser Val Ser Lys Asp

35 40 45

Asp Leu Phe His Val Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala

65 70 75 80

Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Val Asp Glu Glu Ile Gly Met Leu His Gln Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Met Thr Tyr Val Gly Arg Thr Thr Lys His Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Ser Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Met Asn Lys Val

225 230 235 240

Thr Glu Ala Arg Phe Leu Leu Gln Ala Val Thr Ala Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Val Lys Ser Tyr Glu Arg Ala Thr Gln

260 265 270

Met Leu Gln Glu Met Glu Ser Thr Ala Pro Ser Val Asp Ser Val Lys

275 280 285

Asp Lys Gly Asp Asn Lys Phe Asn Glu Thr Glu Arg Phe Ser Gly Arg

290 295 300

Asn Met Ser Ser Pro Tyr Ser Thr Pro Asn Leu Glu Ser Ser Asn Gly

305 310 315 320

Lys Thr Thr Gly Thr Val Lys Ser Arg Thr Glu Asn Asn Arg Ser Leu

325 330 335

Thr Ser Asp Ala Lys Asp Ser His His Ser His Ala Arg Arg Arg Leu

340 345 350

Tyr Glu Ser Leu Asp Pro Ala Lys Ser Asp Pro Lys Val Pro Pro Tyr

355 360 365

Thr Lys Pro Lys Arg Pro Ser Trp Gly Phe Asn Ser His Ser Asp Ser

370 375 380

Lys Pro Ser Phe Val Ser Tyr Pro Asn Glu Lys Ala Pro Tyr Ile Ile

385 390 395 400

Lys Pro Asn Ser Thr Gln Asn Gly Phe Ser Pro Arg Thr Ala Thr Asn

405 410 415

Trp Arg Thr Arg Thr Pro Glu Gly Asp Ala Ala Ile Val Lys Tyr Gly

420 425 430

Pro Thr Thr Thr Val Lys Gln Gly Asn Lys Thr Thr Val Phe Ser Ser

435 440 445

Gly Ser Ile Tyr Pro Leu Asn Thr Glu Ala Ala Met Lys Phe Thr Lys

450 455 460

Asn Asp Asn Asn Asn Lys Phe Thr Val Thr Asn Glu Phe Ala Ala Ser

465 470 475 480

Val Asp Thr Lys Asp Gln Asn Gln Asp Lys Lys Leu Ala Lys Lys Ser

485 490 495

Trp Ala Asp Met Val Glu Glu Glu Gln Gln Ser Glu Glu Tyr Glu Leu

500 505 510

Phe Tyr Lys Gly Tyr Thr Asn Phe Asp Ala Gln Val Phe Gln Asn Glu

515 520 525

Asn Glu Asn Ser Asn Ile Val Tyr Gln Pro Pro Ser Arg Arg Ser His

530 535 540

Tyr Glu Thr Glu Ser Leu Asn Gln Asn Leu Glu Phe Met Asn Leu Lys

545 550 555 560

Asp Gly Tyr Asn Ala Ala Pro Val Asn Asp Thr Trp Leu Arg Asn Pro

565 570 575

Thr Val Arg Arg Ser Leu Phe Thr Asn Ala Glu Met Thr Asn Glu Arg

580 585 590

Asp Val Phe Ser Gly Glu Glu Lys Arg Thr Arg Arg Ala Arg Leu Gln

595 600 605

Val Phe Gln Asp Ile Thr Ser Ser Ser

610 615

673

PRT

葡萄（Vitis vinifera）

Met Trp Ser His Asn Asn Asn Phe Pro Ala Lys Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Thr Trp Lys Ser Lys Ala Ser Phe Ser Ser Ala Thr Pro Val

20 25 30

Ser Glu Lys Thr Arg Ser Met Ala Asn Lys Asp Asp Leu Phe His Val

35 40 45

Val His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg His Leu Cys Pro Gln Glu Ser Gln Glu Ser Leu

115 120 125

Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Leu Asp Glu

130 135 140

Gln Ile Glu Met Leu Gln Tyr Lys Leu Lys Asn Ile Asp Glu Gly Ser

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Ile Gln Ile Ser Ile Glu Gln Glu Lys Ser Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Gly Glu Leu

195 200 205

Tyr Lys Gln Ala Leu Ala Leu Asp Pro Asp Arg Asn Lys Glu Cys Asn

210 215 220

Leu Ala Ile Cys Leu Met Tyr Met Asn Lys Ile Lys Glu Ala Lys Ala

225 230 235 240

Met Leu Tyr Ala Ile Gln Val Ser Ser Gln Asn Gly Arg Met Asp Asp

245 250 255

Ser Tyr Val Lys Ser Phe Glu Arg Ala Ser Gln Val Leu Thr Glu Leu

260 265 270

Glu Ala Asn Ser Val Ile Asp Pro Asn Glu Gln Glu Gly His Glu Glu

275 280 285

Met Arg Arg His Leu Arg Ser Leu Val Ser Arg Asn Ser Ile Glu Val

290 295 300

Asn Ser Cys Ile Asn Glu Glu Asn Asp His Leu Ser Gly Leu Val Ala

305 310 315 320

Ser Arg Arg Arg Ala Gly Arg Gln Gln Glu Glu Thr Met Leu Leu Asp

325 330 335

Lys Pro Asn Arg Arg Ser Tyr Cys Gln Asn Gln Phe Glu Asn Lys Asp

340 345 350

Asn Phe Ser Gln Pro Asp Glu Glu Ser Ser Lys Cys Met Ser Leu Gly

355 360 365

Leu Ser Ser Ala Gln Ser Pro Gln Asn Leu Tyr Ala Asp Lys Trp Lys

370 375 380

Lys Gly Ala Gln Leu Glu Asn Pro Phe Glu Arg Ser Asp Phe Ser Ser

385 390 395 400

Arg Arg Lys Gly Asn Trp Val Ser Ala Thr Asp Lys Val Gly Ser Val

405 410 415

Gln Arg Arg Thr Tyr Gly Ser Pro Leu Pro Val Arg Gly Asn Ser Lys

420 425 430

Leu Pro Ser Thr Glu Gln Arg Arg Gly Pro Cys Leu Leu Ser Lys Ala

435 440 445

Asp Gln Arg Lys Ser Thr Trp Gly Glu Asn Thr Ala Asp Ser Pro Gly

450 455 460

Arg Lys Leu Ser Phe Glu Asp Pro Ile Ala Lys Glu Ala Gly Ala Met

465 470 475 480

Ala Pro Gln Asn Pro Asp Gly Arg Leu Gln Ala Ser Ser Asn Glu Lys

485 490 495

Leu Lys Ile Ala Leu Gln Thr Ser Glu Lys Ser Leu Pro Ser Pro Gly

500 505 510

Gly Phe Asp Gly Lys Cys Phe Arg Glu Asn Ser Gly Lys Leu Met Ser

515 520 525

Leu Gln Gln Val Glu Gly Asn Pro Gln Leu Pro Asn Gln Asp Ser Ser

530 535 540

Thr Ser Lys Asn Lys Met Ser Trp Ala Asp Met Leu Asp Tyr Cys Tyr

545 550 555 560

Gln Lys Pro Ser Phe Ser Phe Gln Thr Pro Asn Lys Trp Tyr Asp Gly

565 570 575

Trp Ser His Gly Glu Asp Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile

580 585 590

Phe His Gln Thr Pro Pro Ser Val His Glu Ile Asp Asn Val Ser Tyr

595 600 605

Lys Leu Glu Ala Phe Asp Leu Lys Asp Gly Tyr Asn Thr Pro Gly Ser

610 615 620

Asp Val Ser Ser Arg Asn Asn Pro Thr Ala Arg Arg Ser Leu Ser Asn

625 630 635 640

Glu Thr Met Ser Thr Ser Gly Ser Thr Ile Arg Pro Lys Arg Arg Asn

645 650 655

Arg Leu Gln Val Phe Arg Asp Ile Thr Leu His Thr Glu Ser Pro Arg

660 665 670

Thr

773

PRT

黃瓜（Cucumis sativus）

Met Leu Thr Asn Ser Gly Lys Asn Lys Phe Leu Cys Lys Gly Phe Ser

1 5 10 15

Thr Pro Pro Pro Ser Trp Lys Trp Lys Pro Phe Arg Leu Pro Lys Thr

20 25 30

Ala Pro Phe Ser Glu Ser Lys Arg Leu Ser Pro Asn Phe Ala Asn Lys

35 40 45

Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro

50 55 60

Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Asp Lys Asp Pro Asn Arg

65 70 75 80

Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp

85 90 95

Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser

100 105 110

Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr

115 120 125

Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg

130 135 140

Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Cys Lys Leu Lys

145 150 155 160

Gln Ile Glu Asp Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ala Ala

165 170 175

Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Val Glu Gln Glu Lys Ser

180 185 190

Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asp Asn Ile

195 200 205

Tyr Ile Ala Glu Glu Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp

210 215 220

Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Leu Ile Leu Thr Asn Arg

225 230 235 240

Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly

245 250 255

Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ser

260 265 270

His Met Leu Ala Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Glu His

275 280 285

Glu Glu Asp Asn Asn Thr Ala Ala Thr Ile Thr Ser Lys Asn Thr Thr

290 295 300

Gly Lys Ser Gly Arg Cys Val Pro Gln Ile Thr Ala Ser Thr Lys Trp

305 310 315 320

Thr Arg Asp Asp Glu Arg Met Tyr Ile Asn Glu Asn Ser Trp Asp Asp

325 330 335

Asp His His Trp Asp Cys Tyr Glu Asn Lys Ser Ile Gly Ala Val Asn

340 345 350

Ser Ser His Asn Tyr Leu His Cys Asp Lys Trp Ser Glu Gly Cys Phe

355 360 365

Ile Glu Asn Leu Gly Lys Thr Asp Ser Cys Ile Pro Ile Lys Ile Lys

370 375 380

Gly Asp Arg Asn Gln Gly Gly Leu Phe Arg Leu Glu Asp Glu Ser Phe

385 390 395 400

Asn Cys Cys Ser Leu Phe Ser Ser Pro Thr Pro Ala Lys Arg Ser Val

405 410 415

Glu Val Pro Phe Thr Gln Pro Lys Asn Ser Phe Trp Glu Phe Asn Asn

420 425 430

Arg Trp Gly Ser Lys Glu Arg Lys Gln Gln Arg Lys Arg Ile Arg Lys

435 440 445

Val Leu Phe Gly Asn Pro Ser Lys Lys Asn Lys Ser Phe Asp Ser Gly

450 455 460

Phe Leu Val Asp Ser Ser Ser Glu Ser Glu Gly Thr Lys Pro Thr Ser

465 470 475 480

Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Val Glu Leu

485 490 495

Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Glu Trp Val Met Asn Arg

500 505 510

His Ser Arg Lys Ala Thr Glu Cys Phe Arg Ser Leu Arg Ser Ser Ser

515 520 525

Ser Ser Arg Lys Leu Ser Phe Glu Pro Pro Thr Ser Thr Glu Asn Ile

530 535 540

Gln Thr Thr Asn Asp Ser Asn Phe Gly Arg Phe Glu Leu Ser Arg Ala

545 550 555 560

Val Ser Asp Glu Pro Gln Asp Leu Glu Gly Asp Trp Asn Gln Thr Ser

565 570 575

Cys Gly Asp Ile Lys Tyr Glu Glu Gly Gly Ser Pro Met Leu Tyr Gly

580 585 590

Leu Met Lys Lys Ile Lys Glu Glu Cys Ile Ala Val Asp Gln Lys Leu

595 600 605

Gln His Asn Ser Pro Thr Val Phe Gly Lys Lys Ser Trp Ala Asp Met

610 615 620

Val Glu Glu Glu Glu Glu Glu Lys Glu Met Ser Ser Ser Ser Gly Ser

625 630 635 640

Asp Gln Val Asn Cys Phe Ala Asp Asn Trp Ser Cys Ser Ser Asp Asp

645 650 655

Asn Gly Glu Phe Lys Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile Leu

660 665 670

His Gln Asn His Cys Pro Pro Ser Ser Asn Gln Leu Glu Asp Thr Ile

675 680 685

Lys Ile Gly Ser Leu Glu Ile Lys Asp Asp Ser Asp Glu Val Val Ser

690 695 700

Ser Arg Asn Ser Val Glu Arg Cys Pro Leu Tyr Phe Asp Gln Gln Gln

705 710 715 720

Gln Pro Thr Leu Glu Ser Ile Asp Asn Cys Cys Ala Ser Pro Leu Pro

725 730 735

Arg Lys Asp Leu Thr Thr Glu Val Ser Cys Lys Phe Gly Gln Glu Asn

740 745 750

Lys Leu Met Arg Gly Asn Arg Leu Gln Val Phe His Glu Ile Thr Ser

755 760 765

Val His Gln Glu Ser

770

683

PRT

黃瓜（Cucumis sativus）

Met Trp Thr Asn Asn Ser Lys Asn Asn Phe Pro Cys Lys Gly Phe Leu

1 5 10 15

Thr Pro Pro Pro Ser Trp Lys Ser Gly Pro Phe Arg Ser Pro Lys Thr

20 25 30

Ala Pro Phe Ser Glu Arg Lys Arg Ser Ser Pro Asn Phe Ala Asn Lys

35 40 45

Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro

50 55 60

Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Arg

65 70 75 80

Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp

85 90 95

Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser

100 105 110

Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr

115 120 125

Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg

130 135 140

Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Arg Lys Leu Lys

145 150 155 160

Gln Ile Glu Asp Gly Thr Ile Phe Gly Gly Lys Arg Thr Lys Ala Ala

165 170 175

Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Ile Glu Gln Glu Lys Ser

180 185 190

Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asn Asn Ile

195 200 205

Tyr Val Ala Glu Asp Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ala Asp

210 215 220

Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Gln Ile Leu Thr Asn Arg

225 230 235 240

Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly

245 250 255

Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Phe

260 265 270

His Met Leu Thr Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Gly Asn

275 280 285

Glu Glu Asp Asn Gly Ala Gly Thr Thr Ile Thr Ser Lys Asn Ala Thr

290 295 300

Gly Arg Thr Gly His Cys Val Pro Gln Ile Ala Ala Ser Thr Arg Trp

305 310 315 320

Thr Arg Asp Asp Glu Gln Met Tyr Ile Asn Glu Asn Ser Arg Asp Ile

325 330 335

Asp Pro His Trp Asp Cys Cys Asp Asp Lys Ser Val Gly Ala Val Asn

340 345 350

Ser Ser His Asn Tyr Leu His Ser Asp Lys Trp Ile Glu Gly Cys Cys

355 360 365

Ile Glu Asn Leu Gly Lys Thr Val Ser Cys Met Pro Ile Lys Met Lys

370 375 380

Gly Asn Arg Asn Arg Asp Ser Leu Phe Arg Leu Val Glu Glu Ser Phe

385 390 395 400

Asn Cys Cys Ser Leu His Thr Ser Pro Thr Pro Thr Lys Lys Asn Val

405 410 415

Glu Val Pro Phe Thr Gln Gln Lys Asn Ser Phe Trp Glu Phe Asn Thr

420 425 430

Arg Trp Arg Ser Lys Glu Arg Lys Gln Gln Gln Lys Arg Thr Arg Lys

435 440 445

Val Leu Phe Glu Asn Pro Ser Arg Lys Asp Gln Ser Phe Asp Ser Gly

450 455 460

Phe Val Val Asp Tyr Ser Ser Glu Ser Asp Glu Thr Glu Pro Ala Ser

465 470 475 480

Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Ile Glu Leu

485 490 495

Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Ser Trp Gly Met Asn Gly

500 505 510

Gly Gly Asn Ser Arg Lys Thr Thr Glu Cys Phe Arg Ser Leu Leu Ser

515 520 525

Arg Ser Ser Ser Arg Lys Leu Ser Phe Glu Leu Pro Thr Ser Thr Glu

530 535 540

Asn Thr Gln Ala Met Thr Asp Ser Asn Leu Gly Arg Ser Lys Leu Ser

545 550 555 560

Arg Glu Ile Ser Asp Glu Pro Gln Asp Leu Ala Gly Gly Asp Trp Lys

565 570 575

Gln Thr Ser Tyr Gly Asp Ile Glu Tyr Glu Glu Gly Thr Ile Pro Asn

580 585 590

Asp Ser Met Lys Ile Met Glu Glu His Met Thr Ile Asp His Lys Phe

595 600 605

Lys His Asn Thr Pro Thr Val Gly Gly Lys Lys Ser Trp Ala Asp Met

610 615 620

Val Glu Glu Glu Glu Glu Asp Ser Asp Asp Lys Asn Glu Asp Asp Thr

625 630 635 640

Glu Glu Thr Leu Ser Ser Ser Gly Arg Gly Gln Val Asn Cys Phe Asp

645 650 655

Asp Asn Trp Ser Ser Ser Ser Asp Asn Val Glu Tyr Lys Phe Asn Asp

660 665 670

Glu Thr Leu Thr Val His Gln Glu Leu Glu Cys

675 680

644

PRT

桉樹（Eucalyptus grandis）

Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro

1 5 10 15

Gln Pro Pro Lys Arg Ala Val Arg Pro Leu Val Ser Pro Val Leu Met

20 25 30

Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His

35 40 45

Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln

50 55 60

Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala

65 70 75 80

Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile

85 90 95

Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys

100 105 110

Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn

115 120 125

Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile

130 135 140

Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe

145 150 155 160

Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln

165 170 175

Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp

180 185 190

Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg

195 200 205

Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala

210 215 220

Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu

225 230 235 240

Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr

245 250 255

Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser

260 265 270

Arg Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser

275 280 285

Arg Glu Ser Thr Cys Phe Thr Glu Gln Val Ile Asn Arg Gly Ala Arg

290 295 300

Pro Leu Ala Ser Thr Thr Thr Ala Asn Gly His Gly Glu Glu Thr Trp

305 310 315 320

Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser

325 330 335

Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe

340 345 350

Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu

355 360 365

Asn Cys Leu Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Lys Ser

370 375 380

Ala Tyr Val Ser Pro Ala Ser Ile Lys Arg Asn Ile Glu Phe Ser Pro

385 390 395 400

Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Tyr Ala Ser Pro Ala

405 410 415

Ser Cys Arg Asp Ser Glu Tyr Lys Ser Lys Ala Thr Ser Thr Gly Glu

420 425 430

Thr Lys Leu Val Gly Asn Gly Ser Val Ala Met Ala Lys Pro Lys Asn

435 440 445

Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly Asp Pro Ala Lys Leu Val

450 455 460

Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser Gly Gly Glu Ser Phe Trp

465 470 475 480

Val Asp Lys His Lys Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu

485 490 495

Glu Gln Glu Pro Thr Asp Pro Pro Val Gln His Leu Trp Asn Asp Ser

500 505 510

Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro Thr Arg Ser Phe Arg Ser

515 520 525

Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile Asn Ile Gly Gly His Glu

530 535 540

Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr Leu Thr Phe Ser Asp Leu

545 550 555 560

Gly Cys Gly Ser Lys Ser Gln Thr Met Asp Thr Pro Ser Arg Thr Ser

565 570 575

Gly Arg Ser Ala Val Arg Arg Ser Leu Asn Phe Asn Gln Ile Pro Lys

580 585 590

Gln Glu Leu Asp Ser Val Met Leu Leu Glu Pro Glu Lys Ala Gly Gly

595 600 605

Cys Gly Ala Pro Glu Asp Ala Val Pro Val Lys Lys Ala Gly Gln Pro

610 615 620

Pro Arg Arg Lys Arg Leu Gln Val Phe Gln Asp Ile Thr Arg Leu Pro

625 630 635 640

Pro His Thr Ala

634

PRT

桉樹（Eucalyptus grandis）

Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro

1 5 10 15

Gln Pro Pro Lys Arg Ala Thr Arg Pro Leu Val Ser Pro Val Leu Met

20 25 30

Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His

35 40 45

Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln

50 55 60

Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala

65 70 75 80

Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile

85 90 95

Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys

100 105 110

Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn

115 120 125

Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile

130 135 140

Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe

145 150 155 160

Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln

165 170 175

Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp

180 185 190

Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg

195 200 205

Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala

210 215 220

Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu

225 230 235 240

Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr

245 250 255

Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser

260 265 270

Leu Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser

275 280 285

Arg Glu Ser Thr Cys Cys Thr Glu Arg Val Ile Asn Leu Gly Ala Gln

290 295 300

Pro Leu Ala Ser Thr Thr Ile Ala Asn Gly His Gly Glu Glu Thr Trp

305 310 315 320

Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser

325 330 335

Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe

340 345 350

Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu

355 360 365

Asn Cys His Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Arg Ser

370 375 380

Ala Tyr Ala Ser Pro Ala Ser Val Lys Arg Asn Ile Glu Phe Ser Pro

385 390 395 400

Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Ile Gln Val Glu Gly

405 410 415

Tyr Phe Asn Trp Glu Arg Gln Asn Ser Ser Glu Met Gly Ser Val Ala

420 425 430

Met Ala Lys Pro Lys Asn Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly

435 440 445

Asp Pro Ala Lys Val Val Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser

450 455 460

Gly Gly Glu Ser Phe Trp Val Asp Lys His Lys Lys Ser Trp Ala Asp

465 470 475 480

Met Ala Glu Glu Glu Glu Glu Gln Glu Pro Thr Asp Pro Pro Val Gln

485 490 495

His Leu Trp Asn Asp Ser Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro

500 505 510

Thr Arg Ser Phe Arg Ser Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile

515 520 525

Asn Ile Gly Gly His Glu Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr

530 535 540

Leu Thr Phe Ser Asp Leu Gly Cys Gly Ser Lys Ser Gln Thr Met Asp

545 550 555 560

Thr Pro Ser Arg Thr Ser Gly Arg Ser Ala Val Arg Arg Ser Leu Asn

565 570 575

Phe Asn Gln Ile Pro Lys Gln Glu Leu Asp Ser Val Met Leu Leu Glu

580 585 590

Pro Glu Lys Ala Gly Gly Cys Gly Ala Pro Glu Asp Ala Val Pro Val

595 600 605

Lys Lys Ala Gly Gln Pro Pro Arg Arg Lys Arg Leu Gln Val Phe Gln

610 615 620

Asp Ile Thr Arg Leu Pro Pro His Thr Thr

625 630

689

PRT

藍花耬鬥菜（Aquilegia caerula）

Met Gly Ile Asp Asn Lys Lys Val Gly Met Lys Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Pro Pro Leu Arg Trp Lys Ser Ala Pro Cys Thr Pro Ile Gln

20 25 30

Thr Leu Glu Met Asn Glu Glu Ser Leu His Gln Leu Ser Ala Thr Ile

35 40 45

Ala Lys Val Asp Ser Phe His Ile Ile His Lys Val Pro Ser Gly Asp

50 55 60

Ser Pro Tyr Val Lys Ala Lys His Val Gln Leu Ile Asp Lys Asp Pro

65 70 75 80

Ser Lys Ser Val Ala Leu Phe Trp Ser Ala Ile Asn Ala Gly Asp Arg

85 90 95

Val Asp Ser Ala Leu Lys Asp Met Ala Ile Val Met Lys Gln Leu Asp

100 105 110

Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys

115 120 125

Thr Pro Leu Ser Gln Glu Ser Leu Asp Asn Val Leu Val Asp Leu Tyr

130 135 140

Lys Arg Gly Gly Arg Ile Glu Glu His Ile Glu Leu Leu His His Lys

145 150 155 160

Leu Lys Leu Ile Asp Asp Gly Val Ala Phe Gly Gly Lys Lys Thr Lys

165 170 175

Ile Ala Arg Ser Gln Gly Lys Lys Phe Leu Val Ser Ile Asp Gln Glu

180 185 190

Arg Ser Arg Leu Leu Gly Asn Leu Gly Trp Ala Tyr Met Gln Gln Asn

195 200 205

Asp Tyr Lys Thr Ala Glu Glu Ile Tyr Arg Lys Ala Leu Ser Ile Glu

210 215 220

Gln Asp Lys Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Asn Arg

225 230 235 240

Gly Glu Ile Met Glu Ala Lys Ser Leu Leu Gln Thr Val Thr Pro Ser

245 250 255

Ser Thr Glu Arg Glu Leu Val Asp Pro Phe Ile Lys Ser Phe Asp Arg

260 265 270

Ala Tyr Glu Met Leu Ile Glu Leu Glu Ser Lys Ser Cys Pro Asn Arg

275 280 285

Asn Ala Lys Pro Val Ala Ser Ala Ser Pro Ile Glu Glu Pro Leu Leu

290 295 300

Leu Lys Lys Gly Ser Tyr Val Cys Asp Asp Arg Leu Asp Ser Phe Gly

305 310 315 320

Lys Gly Arg Leu Asn Arg Glu Val Glu Met Glu Glu Lys Gly Gly Gly

325 330 335

His Ser Cys Arg Lys Ser Leu Phe Ala Asp Lys Gly Asn Lys Glu Asn

340 345 350

Cys Ser Pro Asn Leu Phe Glu Arg Gly Asn Gly Ile Ser Gly Trp Lys

355 360 365

Asn Val Glu Pro Phe Ser Glu Ser Arg Met Glu Glu Gly Ser Thr Arg

370 375 380

Lys Asn Val Arg Thr Phe Gly Phe His Arg Tyr Ser Asn Arg Ser Pro

385 390 395 400

Lys Leu Thr Thr Lys Tyr Gly Leu Gln Ser Pro Ser Asp Gly Asp Trp

405 410 415

Arg Arg Arg Ser Arg Asp Asn Asp Ala Asn Lys Asn Gln Ala Thr Ile

420 425 430

Thr Pro Thr Lys Ser Pro Ile Phe Ile Glu Ser Lys Cys Gly Leu Gln

435 440 445

Ser Thr Asp Gly Gly Cys Arg Lys Ser Pro Ile Ser Phe Ala Tyr Gln

450 455 460

Lys Ala His Glu Val Gly Gly Asp Leu Gln Ala Cys Ser Gly Gln His

465 470 475 480

Leu Glu Gln Arg Ser Glu Val Thr Val Thr Asp Ser Ala Ser Lys Tyr

485 490 495

Ile Gly Gly Asn Glu Thr Pro Glu Ala Cys Asn Gly Ile Asp Ser Thr

500 505 510

Thr Glu Gly Ser Thr Ser Lys Tyr Leu Cys Ser Ser Phe Arg Ser Gly

515 520 525

Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu Glu Leu Leu Arg Glu

530 535 540

Cys Lys Asn Asn Ser Ile Ser Thr Trp Glu Tyr Pro Ser Phe Gln Thr

545 550 555 560

Pro Cys Lys Ser Val Asp Arg Trp Asn Asp Glu Lys Phe Phe His Asn

565 570 575

Glu Asn Met Asp Ser Asn Ile Ile Gly Ser Thr Pro Glu Leu Thr Thr

580 585 590

Gln Pro Arg Arg Asp Gly Tyr Thr Gln Thr Leu Ile Arg Lys Leu Glu

595 600 605

Leu Ile Asp Leu Lys Lys Glu Glu His Ile Lys Thr Thr Ala Ser Tyr

610 615 620

Ser Phe Ser Asn Pro Thr Ala Arg Arg Thr Leu Ala Phe Glu Lys Thr

625 630 635 640

Asp Ser Ala Asp Tyr Phe Arg Pro Ser Pro Leu Cys Lys Gln Val Leu

645 650 655

Phe Asn Gly Glu Ser Asp Asn Thr Ala Gly His Gly Lys Asn Ser Met

660 665 670

Pro Val Gly Arg Arg Ser Arg Arg Leu Pro Val Phe Gln Glu Ile Thr

675 680 685

Pro

625

PRT

菜豆（Phaseolus vulgaris）

Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Phe Val Thr Pro Pro

1 5 10 15

Pro Pro Ser Pro Trp Lys Thr Ile Ser Ser Arg Ser Pro Ser Val Pro

20 25 30

Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ser Asp Ile

35 40 45

Phe His Ile Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Lys

50 55 60

Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala Ile Ser

65 70 75 80

Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala Leu

85 90 95

Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala

100 105 110

Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp Ser Gln

115 120 125

Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Phe Lys Arg Ser Gly Arg

130 135 140

Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Leu Ile Glu

145 150 155 160

Asp Gly Ile Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser Gln

165 170 175

Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg Ile Leu

180 185 190

Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Glu Asp Tyr Lys Thr Ala

195 200 205

Glu Glu His Tyr Arg Arg Ala Leu Ser Phe Glu Val Asp Arg Asn Lys

210 215 220

Gln Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Lys Glu

225 230 235 240

Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn Arg Lys

245 250 255

Met Asp Glu Ser Phe Val Lys Ser Phe Glu Arg Ala Ser Gln Met Leu

260 265 270

Val Glu Ile Glu Thr Ser Ser Ser Glu Asn Ala Ser Phe Ser Met Thr

275 280 285

Thr Ser Leu Pro Gln Cys Ser Ser Leu Gly Phe Glu Asn Ser Val Arg

290 295 300

Lys Ser Ser Asp Arg Val Lys Arg Thr Glu Asn Gln Ser Asp Lys Ser

305 310 315 320

Glu Gly Asp Val Ser His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp

325 330 335

Pro Gly Arg Arg His Leu Asn Leu Tyr Val Pro Thr Lys Pro Lys Arg

340 345 350

Cys Ser Trp Gly Phe Asn Asn Gly Tyr Arg Arg Gly Asp Phe His Ser

355 360 365

Asp Ser Lys Pro Ser Ser Gly Thr Pro Pro Asn Glu Lys His Val Thr

370 375 380

Arg Thr Leu Asn Leu Arg Glu Ser Gly Leu Ser Ser Pro Ala Asn Glu

385 390 395 400

Lys Trp Arg Thr Ser Thr Leu Glu Asp Pro Ala Ile Arg Lys Asn Lys

405 410 415

Asp Thr Lys Val Ile Ile Ser Asp Leu Leu His Thr Leu Asn Thr Glu

420 425 430

Ala Val Val Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Gly Asp Ser

435 440 445

Ser His Arg Ser Ile Met Ser Glu Ser His Ala Met Val Glu Asn Gly

450 455 460

Thr Asp Asp Phe Ala Ser Gly Asn Gly Lys Pro His Glu Lys Lys Ser

465 470 475 480

Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe Phe

485 490 495

Ser Gly Tyr Ile Asn Phe Asp Gly Glu Asn Gly Ala Glu Val Phe Asn

500 505 510

Asp Glu Asn Glu Asp Ser Asn Ile Met Tyr Gln Ser Pro Trp Pro Gln

515 520 525

Asn Gln Pro Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Glu Gln Lys

530 535 540

Asp Gly Tyr His Pro Ser Gly Ser Val Ile Leu Ser Arg Asn Pro Thr

545 550 555 560

Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ser Ser Glu Ser Ala

565 570 575

Tyr Ala Ile Arg Thr Ser Lys Ser Pro Lys Lys Ala Ser Asn Leu Glu

580 585 590

Asn Arg Asp Thr Leu Val Gly Glu Lys Lys Leu Pro Arg Lys Ser Arg

595 600 605

Leu Gln Val Phe Gln Asp Ile Thr Leu Phe Pro Glu Thr Pro Arg Phe

610 615 620

Ala

625

758

PRT

柿樹（Prunus persica）

Met Trp Thr Arg Asp Lys Ser Leu His Thr Arg Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Thr Trp Lys Ser Gly Pro Pro Asn Pro Pro Met Val Pro Met

20 25 30

Ser Glu Arg Lys Arg Val Ser Pro Ser Asp Gly Gly Asp Leu Phe His

35 40 45

Val Met His Lys Val Pro Val Gly Asp Ser Pro Tyr Val Arg Ala Lys

50 55 60

Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Ile Val Met Lys Gln Leu Asn Arg Ser Glu Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu Ser

115 120 125

Leu Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu

130 135 140

Glu Glu Ile Glu Met Leu Gln Ser Lys Leu Lys His Ile Asp Glu Gly

145 150 155 160

Ile Ala Phe Gly Gly Arg Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys

165 170 175

Lys Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Glu Glu

195 200 205

Tyr Tyr Met Lys Ser Leu Ser Leu Glu Leu Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Ala Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Val Val Arg Ala Ser Ser Gly Asn Lys Pro Met Asp

245 250 255

Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ile Gln Met Leu Thr Glu

260 265 270

Leu Glu Ala Lys Ser Val Leu Arg Pro Ile Gln Leu Asp Glu Asn Cys

275 280 285

Cys Lys Glu Ile Ser Arg Phe Pro Ile Ser Pro Ile Asn Arg Asn Ser

290 295 300

Lys Gln Gly Asn Ser Leu Thr Asn Glu Gly Gln His Tyr Val Ser Gly

305 310 315 320

Cys Met Ile Ser Arg Arg Trp Ala Asp Gly His Glu Glu Glu Thr Val

325 330 335

Ser Val Asn Lys Trp Lys Lys Asp Cys Tyr Phe Lys Asn Ser Cys Glu

340 345 350

Gly Arg Ser Ser Phe Ser Ser Arg Met Lys Glu Asn Gln Gly Gly Ile

355 360 365

Val Gly Thr Glu Thr Thr Pro Tyr Ser Lys Thr Phe Phe Ser Pro Ala

370 375 380

Pro Asp Ile Trp Asn Arg Glu Val Leu Phe Thr Gln Pro Arg Arg Ser

385 390 395 400

Ser Arg Gly Phe Asn Asp Gly His Gln Thr Arg Glu Ile Trp Gly Arg

405 410 415

Gly Val Gly Ser Ser Asn Lys Lys Leu Ser Phe Glu Ser Cys Ser Arg

420 425 430

Thr Glu Asn Met Arg Ala His Val Val Arg Ser Leu Asn Glu Asp Leu

435 440 445

Leu Ala Ser Thr Thr Gly Lys Ser Glu Val Ala Phe Gln Asn Ser Val

450 455 460

Ser Ser Ile Ser Ser Pro Ile Ser Arg Asp Leu Arg Arg Arg Pro Gln

465 470 475 480

Lys Asp Ala Ala Val Arg Ser Val Leu Gln Pro Ile Ser Ser Gly Asn

485 490 495

Trp Lys Cys Thr Ser Arg Ala Asn Asp Gly Cys Phe Gln Leu Lys Asp

500 505 510

Glu Ala Val Val Val Ser Ser Ser Gln Asn Thr Val Asn Gly Asp Trp

515 520 525

Arg Arg Thr Ser Trp Glu Asn Asp Gly Met Lys Lys Ser Ala Glu Pro

530 535 540

Leu Met Val Gly Glu Asp Ala Lys Ala Leu Glu Ile Ser Thr Asp Gly

545 550 555 560

Gly Pro Asn Gln Ser Ser Asp Thr Thr Ala Phe Val Glu Asp Cys Phe

565 570 575

Gly Glu Asn Thr Ser Ser Lys Val Asp Asp Val His Gln Pro Ile Ala

580 585 590

Glu Asn Gln Lys Pro Ala Pro Asp Phe Ser Met Tyr Ser Lys Cys Lys

595 600 605

Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Gln Glu Leu Leu Asn

610 615 620

Gly Arg Thr Glu Tyr Phe Asp Ser Trp Tyr Thr Glu Asp Gly Phe Asn

625 630 635 640

Asn Glu Asn Leu Asn Cys Asn Ile Thr Pro Glu Ser Pro Cys Leu Gln

645 650 655

Ser Gln Met Lys Ser Leu Gly Gln Lys Leu Gln Ser Thr Asp Leu Val

660 665 670

Asp Glu Tyr Val Ser Gly Asn Ala Ala Ser Ser Arg Asn Ser Thr Val

675 680 685

Arg Arg Ser Leu Cys Phe Gly Gln Gln Gln Glu Gln Glu Ser Val Asp

690 695 700

Tyr Ile Ser Ser Ser Pro Val Pro Lys Glu Ala Leu Asn Phe Glu Gly

705 710 715 720

Ser Asp Ser Val Gln Ala Asn Gly Lys Gly Ser Ile Tyr Gly Lys Asn

725 730 735

Ser Ser Phe Ser Arg Arg Lys Arg Leu Gln Ser Phe Gln Asp Ile Thr

740 745 750

Glu Phe Gln Asp Ser Pro

755

649

PRT

雷蒙德氏棉（Gossypium raimondii）

Met Trp Ser Ser Asp Lys His Cys Pro Ala Arg Gly Phe Leu Thr Pro

1 5 10 15

Gln Pro Pro Ala Trp Lys Lys Val Gln Ser Thr Thr Val Leu Pro Met

20 25 30

Ser Glu Arg Lys Arg Ile Ser Pro Ala Asn Asn Gly Asp Cys Phe His

35 40 45

Val Ile His Lys Val Pro Ala Ser Asp Ser Pro Tyr Gly Arg Ala Lys

50 55 60

His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Val Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Asp Ser Gln Glu Ser

115 120 125

Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val Asp

130 135 140

Glu Glu Ile Glu Ile Leu Leu Asn Lys Leu Arg Asn Ile Glu Glu Gly

145 150 155 160

Thr Val Phe Gly Gly Lys Lys Thr Lys Ile Ala Arg Ser Gln Gly Lys

165 170 175

Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu Gln

195 200 205

His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Gly Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Asp Val Lys Val Ser Ala Gly Thr Glu Glu Met Asp

245 250 255

Glu Ser Tyr Ser Lys Ser Tyr Glu Arg Ala Met Glu Ile Leu Met Gln

260 265 270

Val Glu Thr Gln Ser Lys Leu Glu Pro Ala Gly Ala Gln Glu Pro Glu

275 280 285

Lys Gly Asn Glu Thr Arg Arg Cys Leu Thr Ser Cys Arg Asp Arg Ser

290 295 300

Leu Lys Glu Ala Ser Val Phe Leu Pro Arg Asn Gly Asp Asn Ile Pro

305 310 315 320

Trp Cys Ile Glu Lys Asn Gly Asn Leu Ser Gly Tyr Asp Asp Thr Ser

325 330 335

Ser Ser Gln Cys Thr Pro Ile Gly Leu Lys Gly Ser Phe Gln Cys Ser

340 345 350

Pro Gln Thr Met Leu Ser Glu Lys Trp Arg Lys Gly Ser Tyr Phe Glu

355 360 365

Ser Pro Ser Glu Gly Ser Val Tyr Ser Ser Ser Lys Leu Lys Glu Ser

370 375 380

Trp Arg Tyr Ser Ala Gly Gln Glu Val Gly Ser Ala His Lys Asn Met

385 390 395 400

Tyr Ala Ser Leu Ala Ala Ser Arg Lys Asn Ser Glu Lys Val Leu Leu

405 410 415

Thr Gln Pro Arg Arg Cys Ser Trp Gly Phe Asn Thr Ala Asp Gln Arg

420 425 430

Arg Gly Gly Arg Trp Gly Glu Asp Thr Thr Val Arg Asn Ser Ile Arg

435 440 445

Lys Leu Ser Phe Glu Gln Thr Thr Thr Thr Glu Ser Val Pro Ser Pro

450 455 460

Ser Ile Gln Lys Leu Lys Glu Glu Pro Leu Ser Ser Ser Asn Ala Lys

465 470 475 480

Ser Glu Asn Tyr Ser Ala Val Gly Leu Gly Glu Glu Glu Ala Gln Glu

485 490 495

Gly Leu Ser Gly Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Ser Trp

500 505 510

Leu Asn Asn Arg Asp Gln Arg Arg Gly Arg Cys Ala Glu Glu Ser Ile

515 520 525

Asp Gly Ser Phe Ser Lys Leu Ser Ser Ser Val Thr Thr His Ser Val

530 535 540

Gln Ser Leu Asn Val Glu Pro Leu Val Ser Ser Lys Asp Glu Ser Glu

545 550 555 560

Ile Gly Val Glu Lys Pro Ala Asp Ala Ala Ser Asn Lys Lys Thr Trp

565 570 575

Ala Asp Met Val Glu Glu Glu Glu Lys Asp Glu Phe Leu Asn Asp Glu

580 585 590

Asn Leu Asn Ser Asn Ile Ile Tyr Gln His Pro Asp Arg Ser Lys His

595 600 605

His Ile Glu Asn Ile Thr Gln Gln Leu Glu Ser Phe Gly Val Lys Gly

610 615 620

Gly Tyr Asn Ala Ser Ala Asn Thr Val Ser Leu Arg Arg Asn Arg Leu

625 630 635 640

Gln Val Phe Arg Asp Ile Thr Ser Thr

645

614

PRT

番茄（Solanum lycopersicum）

Met Trp Arg Asn Asn Glu Arg Val Tyr Met Thr Pro Ala Arg Gly Phe

1 5 10 15

Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Thr Glu Lys Asp Gln

20 25 30

Lys Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val

35 40 45

Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Val Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile

115 120 125

Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu

130 135 140

Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr

195 200 205

Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe

225 230 235 240

Leu Leu Gln Ser Ile Lys Thr Ser Asp Arg Arg Gln Met Asp Glu Ser

245 250 255

Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu

260 265 270

Ser His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu

275 280 285

Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Arg Arg Gly His

290 295 300

Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His

305 310 315 320

Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly

325 330 335

Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe

340 345 350

Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn

355 360 365

Phe Asn Lys Leu Ile Ser Val Asn Asp Ile Ser Glu Gly Ala Ser Val

370 375 380

Val Cys Gly Gln Val Phe Ser Arg Ser Trp Gly Asn Gly Ala Asn Val

385 390 395 400

Arg Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn

405 410 415

Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Arg

420 425 430

Ser Pro Thr Glu Ser Leu Pro Asp Ile Thr Ser Ala Arg Lys Cys Ser

435 440 445

Glu Asn Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp

450 455 460

Met Val Thr Leu Glu Asp Thr Thr Glu His Leu Asp Ser Thr Ser Leu

465 470 475 480

Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp

485 490 495

Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly

500 505 510

Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ala Asn Ile Ile Asn Leu

515 520 525

Ser Gln Asn Ile Asp Thr Leu Cys Leu Asn Glu Gly Tyr His Thr Gln

530 535 540

Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp His Asn Asp Arg

545 550 555 560

Lys Glu Lys Cys Ser Ser Gly Phe Gln Gly Lys Glu Leu Lys Ser Gly

565 570 575

Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro

580 585 590

Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr

595 600 605

Pro Glu Ser Pro Lys Pro

610

614

PRT

馬鈴薯（Solanum tuberosum）

Met Trp Arg Asn Asn Glu Arg Val Tyr Met Ser Pro Ala Arg Gly Phe

1 5 10 15

Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Ser Asp Lys Asp Gln

20 25 30

Arg Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val

35 40 45

Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile

115 120 125

Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu

130 135 140

Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr

195 200 205

Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe

225 230 235 240

Leu Leu Gln Ser Ile Lys Ala Ser Asp Arg Trp Gln Met Asp Glu Ser

245 250 255

Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu

260 265 270

Thr His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu

275 280 285

Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Cys Arg Gly His

290 295 300

Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His

305 310 315 320

Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly

325 330 335

Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe

340 345 350

Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn

355 360 365

Phe Asn Lys Leu Ile Ser Ala Asn Asp Met Ser Glu Gly Ala Ser Leu

370 375 380

Val Arg Gly Gln Val Leu Thr Arg Ser Trp Gly Asn Gly Ala Asn Val

385 390 395 400

Glu Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn

405 410 415

Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Lys

420 425 430

Ser Pro Thr Glu Ser Leu Ala Asp Ile Thr Ser Ala Arg Lys Tyr Ser

435 440 445

Gly Asp Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp

450 455 460

Thr Val Thr Leu Glu Asp Thr Thr Glu His Leu Glu Ser Thr Asn Leu

465 470 475 480

Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp

485 490 495

Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly

500 505 510

Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ser Asn Ile Ile Asn Leu

515 520 525

Ser Gln Asn Ile Asp Thr Leu Arg Leu Asn Glu Gly Tyr His Thr Gln

530 535 540

Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp Gln Asn Asp Arg

545 550 555 560

Lys Glu Lys Cys Ser Ser Asp Phe Gln Gly Lys Val Leu Lys Ser Gly

565 570 575

Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro

580 585 590

Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr

595 600 605

Pro Glu Ser Pro Lys Pro

610

683

PRT

粟（Setaria italica）

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Pro Arg Trp Ser Pro Ala Ala Gly Thr Pro Val Ala Gly

20 25 30

Ala Gly Cys Gly Pro Val Ser Gly Tyr Arg Thr Pro Pro Val Ser Ala

35 40 45

Gly Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Ala

50 55 60

Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro Arg

65 70 75 80

Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln

85 90 95

Val Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala

100 105 110

Ile Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Asn

115 120 125

Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg

130 135 140

Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Asp Ser Leu Asp Asn

145 150 155 160

Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Glu Glu Gln Ile

165 170 175

Glu Met Leu Thr Ile Lys Leu Arg Val Val Asp Glu Glu Leu Ala Ser

180 185 190

Gly Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr

195 200 205

Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp

210 215 220

Ala Tyr Met Gln Ser Glu Asn Tyr Glu Glu Ala Glu Met Leu Tyr Arg

225 230 235 240

Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala

245 250 255

Ile Cys Leu Met Lys Thr Gly Lys Leu Ala Glu Ala Lys Tyr Leu Leu

260 265 270

Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser Leu

275 280 285

Ser Arg Ala Thr Glu Met Leu Arg Asp Leu Glu Leu Gln Ser Leu Pro

290 295 300

Ser Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Arg Ile Leu Leu Ala

305 310 315 320

Thr Asp Val Glu Ile Leu Glu Asp Pro Gln Pro Gln Thr Leu Ser Thr

325 330 335

Pro Leu Ser Gln Leu Lys Tyr Lys Glu Pro His Ile Ser Val Ser Ala

340 345 350

Asn Ala Glu Gln His Glu Lys Cys Ser Ser Trp Phe Pro Ser Pro Ile

355 360 365

Thr Gln Leu Lys Arg Glu Glu Pro Arg Ile Leu Val Thr Val Asp Ala

370 375 380

Glu Lys Asn Glu Gly Cys Ala Glu Phe Gln Asp Leu Ser Arg Leu Phe

385 390 395 400

Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys Arg

405 410 415

Leu Val Asn Glu Ala Pro Lys Ser Ser Ile His Asp Gln Ile Gln Thr

420 425 430

His Thr Pro Thr Glu Cys Leu Pro Asn Ser Glu Gly Asn His Asn Ala

435 440 445

Ser Glu Asn Pro Val Gln Gly Gly Lys Leu Leu Thr Lys Gly Val Arg

450 455 460

Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu Gly Glu

465 470 475 480

Asp Lys Ser Trp Thr Asp Met Val Ala Lys Gly Glu His Gln Leu Arg

485 490 495

Asn Asp Lys Leu Thr Val Gly Val Gly Thr Thr Glu Gln Thr Glu Ser

500 505 510

Ser Lys His Ala Ser Lys Gln Glu Tyr Arg Thr Pro Pro Pro Ser Gln

515 520 525

Gly Ser Ser Thr Leu His Arg Pro Val Ile Gly Gly His Gln Gln Gly

530 535 540

Phe Ser Ala Asn Ser Trp Arg Arg Ser Asn Ser Lys Ile Ser Thr Asp

545 550 555 560

Asn Lys Val Asn Trp Asp Leu Val Arg Ala Ala Pro Thr Trp Ser Lys

565 570 575

His Lys Val Gln Asp His Ser Gly Arg Val Cys Gln Arg Pro Asn Ala

580 585 590

Ala His Leu Lys Glu Asn Thr Ser Gly Ser Lys Gln Ala Pro Trp Arg

595 600 605

Ser Ser Ala Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser Lys Gly

610 615 620

Glu Gly Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His Ser

625 630 635 640

Gln Gly Ser Ser Arg Thr Glu Ala Thr His Arg Trp His Asn Asn Ala

645 650 655

Ala Gly Thr Val Ser Trp Arg Pro Gln Asn Arg Leu Arg Val Phe Gln

660 665 670

Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val

675 680

717

PRT

二穗短柄草（Brachypodium distachyon)

Met Arg Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

His Ala Ala Ala Glu Val Val Ala Pro Thr Gly Trp Ser Pro Arg Thr

20 25 30

Pro Ala Ala Gly Gly Gly Gly Ser Gly Ser Tyr Val Thr Pro Pro Leu

35 40 45

Thr Ala Gly Cys Cys Cys Ser Ser Ser Ser Tyr Arg Val Thr Pro Pro

50 55 60

Ser Ser Gly Gly Gly Cys Thr Ser Leu Leu Thr Pro Pro Val Ser Gly

65 70 75 80

Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro Pro Ala Val Val Asp Ser

85 90 95

Pro Tyr Val Arg Ala Lys Gln Ala Gln Leu Ile Glu Lys Asp Pro Asn

100 105 110

Lys Ala Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Glu Arg Ile

115 120 125

Glu Ser Ala Leu Lys Asp Met Ala Thr Val Leu Lys Gln Ala Asn Arg

130 135 140

Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro

145 150 155 160

Asn Glu Ala Gln Asp Ser Leu Asp Asn Val Leu Leu Asp Leu Tyr Lys

165 170 175

Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu Met Leu Thr Ile Lys Leu

180 185 190

Arg Met Val Asp Glu Asp Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu

195 200 205

Ser Lys Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys

210 215 220

Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala His Met Gln Ser Glu Asn

225 230 235 240

Tyr Glu Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala

245 250 255

Asp Tyr Asn Lys Glu Cys Asn Leu Ala Val Cys Leu Met Lys Thr Gly

260 265 270

Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln Ala Ile Pro Tyr Asn Ser

275 280 285

Ser Asp Glu Lys His Val Arg Ser Phe Ala Arg Ala Thr Glu Met Ile

290 295 300

Lys Glu Leu Glu Ser Gln Ala Leu Pro Ser Pro Ile Thr Gln Met Lys

305 310 315 320

Ser Lys Asp Ser Arg Ile Leu Leu Ala Thr Asp Ala Glu Asn Leu Asp

325 330 335

Tyr Ile Gln Pro Glu Ile Leu Ser Thr Ser Ser Thr Gln Leu Gln Tyr

340 345 350

Glu Glu Pro Glu Phe Ser Val Ser Ala Asp Thr Glu Lys Gln Val Asp

355 360 365

Cys Asn Ser Gln Glu Leu Pro Ser Pro Ile Thr Gln Leu Lys Arg Lys

370 375 380

Val Pro Gln Ile Met Val Asp Ser Glu Lys Asn Gly Glu Cys Pro Glu

385 390 395 400

Glu Asn Gln Asp Ile Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro Gln

405 410 415

Ser Leu Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Lys Asp Arg Pro

420 425 430

Asn Ile Ser Ile Gln His Gln Ala Gln Thr Pro Ser Ser Thr Glu Cys

435 440 445

Leu Pro Ile Cys Asn Gly Ala Thr Asp Ala Ser Asp Asn His Leu Gln

450 455 460

Glu Gly Gln Ser Ser Val Gly Gly Ala Arg Lys Thr Trp Ala Asp Met

465 470 475 480

Val Glu Glu Asp Glu Gln Gln Leu Gly Asp Gly Lys Ser Gly Thr Ala

485 490 495

Gln Asn Glu Ser Ser Lys Gln Ala Ser Glu Gln Arg Tyr Ile Thr Pro

500 505 510

Pro Ser Ser Gln Ala Asn Ser Thr Leu Gln Thr Pro Ala Ala Gly Val

515 520 525

Arg Leu Gln Ser Ser Ser Ala Gly Ser Trp Arg Arg Lys Asp Ser Thr

530 535 540

Gly Glu Asn Val Asn Arg Lys Phe Val Arg Thr Ala Pro Ala Trp Arg

545 550 555 560

Gln Gln Lys Val Gln Asp His Ser Asn Arg Val Cys Gln Arg Leu Asn

565 570 575

Thr Ile His Leu Gly Glu Lys Ala Gln Gly Gln Gly Ala Glu Gln Thr

580 585 590

Pro Trp Arg Ser Ser Ala Ala Gln Arg Ser Leu Phe His Gly His Leu

595 600 605

Pro Ser Gly Glu Ser Gly Arg Cys His Val Ser Gly His Thr Glu Ala

610 615 620

Val Asn Arg Trp Pro Lys Asn Ala Ala Ala Ile Thr Arg Pro Trp Arg

625 630 635 640

Ala Gln Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Met Asn

645 650 655

Arg Asn Val Ala Tyr Glu His Cys Cys Leu Pro Cys Met Gln Thr Leu

660 665 670

Cys Ala Cys Thr Phe Trp Arg Tyr Cys Leu Asp Val Thr Ala Trp Phe

675 680 685

Ile Cys Cys Asn Val Leu His Val Ala Phe Ile Cys Tyr Cys Cys Cys

690 695 700

Cys Trp Leu Ala Gly Trp Leu Ala Gly Gln Phe Pro Ala

705 710 715

815

PRT

粳稻（Oryza sativa japonica）

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser

20 25 30

Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser

35 40 45

His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg

50 55 60

Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro

65 70 75 80

Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val

85 90 95

Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile

100 105 110

Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val

115 120 125

Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser

130 135 140

Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile

145 150 155 160

Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu

165 170 175

Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly

180 185 190

Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu

195 200 205

Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala

210 215 220

His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln

225 230 235 240

Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile

245 250 255

Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln

260 265 270

Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala

275 280 285

Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser

290 295 300

Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile

305 310 315 320

Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala

325 330 335

Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp

340 345 350

Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile

355 360 365

Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly

370 375 380

Glu Asn Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val

420 425 430

Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr

435 440 445

Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly

450 455 460

Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu

465 470 475 480

Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val

485 490 495

Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser

500 505 510

Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln

515 520 525

Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr

530 535 540

Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg

545 550 555 560

Gln Gln Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp

565 570 575

Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp

580 585 590

Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys

595 600 605

Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His

610 615 620

Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn

625 630 635 640

Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile

645 650 655

Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile

660 665 670

Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp

675 680 685

Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His

690 695 700

Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala

705 710 715 720

Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp

725 730 735

Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met

740 745 750

Val Val Leu Leu Gln Asp Gln Leu Leu Pro Met Ala Phe Ser Ile Ala

755 760 765

Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser

770 775 780

Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr

785 790 795 800

Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Ala Ala Thr Ile

805 810 815

813

PRT

秈稻（Oryza sativa indica）

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser

20 25 30

Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser

35 40 45

His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg

50 55 60

Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro

65 70 75 80

Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val

85 90 95

Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile

100 105 110

Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val

115 120 125

Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser

130 135 140

Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile

145 150 155 160

Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu

165 170 175

Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly

180 185 190

Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu

195 200 205

Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala

210 215 220

His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln

225 230 235 240

Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile

245 250 255

Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln

260 265 270

Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala

275 280 285

Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser

290 295 300

Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile

305 310 315 320

Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala

325 330 335

Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp

340 345 350

Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile

355 360 365

Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly

370 375 380

Glu Lys Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val

420 425 430

Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr

435 440 445

Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly

450 455 460

Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu

465 470 475 480

Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val

485 490 495

Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser

500 505 510

Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln

515 520 525

Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr

530 535 540

Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg

545 550 555 560

Gln Lys Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp

565 570 575

Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp

580 585 590

Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys

595 600 605

Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Cys Asp Asn Glu His

610 615 620

Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn

625 630 635 640

Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile

645 650 655

Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile

660 665 670

Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp

675 680 685

Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His

690 695 700

Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala

705 710 715 720

Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp

725 730 735

Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met

740 745 750

Val Val Leu Leu Gln Asp Gln Met Leu Pro Met Ala Phe Ser Ile Ala

755 760 765

Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser

770 775 780

Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr

785 790 795 800

Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Thr Ala

805 810

685

PRT

高粱（Sorghum bicolor）

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Gly Ala Ala Arg Trp Ser Pro Ala Ala Gly Thr Pro Ala

20 25 30

Ala Ala Gly Gly Gln Arg Ser Gly Ser Gly Tyr Gly Thr Pro Pro Leu

35 40 45

Ser Ala Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly

50 55 60

Ala Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro

65 70 75 80

Arg Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala

85 90 95

Gln Ile Val Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala

100 105 110

Ala Ile Asn Ser Gly Asp Arg Thr Glu Ser Ala Leu Lys Asp Met Ala

115 120 125

Asn Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile

130 135 140

Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Glu Ser Leu Asp

145 150 155 160

Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Asp Glu Gln

165 170 175

Ile Glu Met Leu Thr Ile Lys Leu Arg Ile Val Asp Glu Glu Leu Ala

180 185 190

Ser Gly Arg Trp Lys Thr Lys Met Ser Lys Ser His Gly Arg Val Val

195 200 205

Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala

210 215 220

Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu Gly Ala Glu Met Leu Tyr

225 230 235 240

Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu

245 250 255

Ala Ile Cys Leu Met Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu

260 265 270

Ile Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser

275 280 285

Leu Ser Arg Ala Thr Glu Met Leu Arg Glu Leu Glu Leu Gln Ser Leu

290 295 300

Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys Glu Ser Gln Ile Phe Leu

305 310 315 320

Ala Asp Asp Val Glu Met Leu Val Asp Leu Gln Pro Gln Thr Leu Ser

325 330 335

Thr Pro Leu Ser Glu Leu Lys Tyr Lys Arg Pro His Ile Ser Val Ser

340 345 350

Gln Asn Ala Glu Lys His Glu Asn Cys Asn Ser Trp Leu Pro Ser Pro

355 360 365

Ile Thr Gln Leu Arg Arg Glu Glu Pro His Ile Met Val Thr Ala Gly

370 375 380

Ala Glu Lys Asn Glu Ser Phe Ala Glu Phe Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Ala Pro Lys Ile Gly Ile His Asp Asp Gln Ile

420 425 430

Gln Thr Pro Ile Pro Thr Glu Cys Leu Pro Asn Ser Glu Arg Asn Leu

435 440 445

Asp Ala Ser Glu Thr Pro Met Gln Glu Gly Lys Leu Leu Thr Lys Gly

450 455 460

Val Lys Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu

465 470 475 480

Gly Asp Asp Lys Pro Leu Ala Asp Met Val Ala Lys Asp Glu Gln Gln

485 490 495

Leu Gly Glu Ser Lys Ser Thr Leu Gly Val Gly Thr Thr Glu Gln Lys

500 505 510

Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg Thr Pro Leu Ala

515 520 525

Ser Gln Glu Ser Arg Thr His Gln Arg Pro Phe Met Gly Gly Gln Leu

530 535 540

Gln Gly Ser Ser Ala Ala Ser Trp Arg Gln Asn Asp Ser Lys Ile Ser

545 550 555 560

Met Asp Lys Asn Val Asn Arg Asp Leu Val Arg Thr Ala Pro Thr Trp

565 570 575

Ser Lys His Lys Ala Gln Asp His Asn Asn Arg Val Trp Gln Arg Leu

580 585 590

Asp Thr Val His Pro His Glu Arg Ala Ser Asp Thr Asn Gln Val Pro

595 600 605

Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser

610 615 620

Lys Gly Glu Gly His Gly His Gly Cys Val Leu Phe Asp Asp Asn Glu

625 630 635 640

Arg Thr Gln Cys Ser Ser His Val Glu Ala Thr His Arg Trp His Asn

645 650 655

Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln Asn Arg Leu Arg Val

660 665 670

Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val

675 680 685

690

PRT

玉米（Zea mays）

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Pro Met Trp Ser Thr Ala Gly Thr Pro Gly Gly Pro Arg

20 25 30

Pro Gly Pro Gly Tyr Gly Thr Pro Pro Val Ser Ala Gly Cys Phe Gly

35 40 45

Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Thr Arg Val Thr Pro Pro

50 55 60

Thr Ser Gly Gly Ala Gly Ala Arg Val Thr Pro Pro Ser Thr Gly Gly

65 70 75 80

Cys Ser Ser Arg Pro Pro Arg Pro Pro Pro Ser Met Asp Ser Pro Tyr

85 90 95

Val Arg Ala Lys Gln Ala Gln Ile Val Glu Lys Asp Pro Asn Lys Ala

100 105 110

Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Asp Arg Ile Glu Ser

115 120 125

Ala Leu Lys Asp Met Ala Asn Val Leu Lys Gln Ala Asn Arg Ser Glu

130 135 140

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu

145 150 155 160

Ala Gln Glu Ser Leu Asp Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys

165 170 175

Gly Arg Thr Asp Glu Gln Ile Glu Met Leu Thr Leu Lys Leu Arg Ile

180 185 190

Val Asp Glu Glu Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu Ser Lys

195 200 205

Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg

210 215 220

Leu Leu Gly Asn Leu Ala Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu

225 230 235 240

Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr

245 250 255

Asn Lys Glu Cys Asn Leu Ala Ile Cys Leu Met Lys Thr Gly Lys Leu

260 265 270

Ala Glu Ala Lys Tyr Leu Ile His Ala Ile Pro Tyr Asn Cys Asn Asp

275 280 285

Glu Ser His Val Lys Ser Leu Ser Arg Ala Thr Glu Met Leu Arg Glu

290 295 300

Phe Asp Leu Gln Ser Leu Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys

305 310 315 320

Glu Pro Arg Asn Phe Val Ala Asp Asp Val Glu Met Leu Val Asp Leu

325 330 335

Gln Pro Gln Thr Leu Ser Thr Pro Phe Ser Glu Leu Lys Tyr Lys Glu

340 345 350

Ala His Ile Ser Val Ser Gln Asn Ala Glu Lys His Glu Asn Cys Asn

355 360 365

Ser Cys Leu Pro Ser Pro Ile Thr Gln Leu Arg Arg Glu Glu Pro His

370 375 380

Thr Met Val Thr Ala Asp Ala Glu Lys Asn Glu Gly Phe Ala Glu Phe

385 390 395 400

Gln Asp Leu Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro His Ser Val

405 410 415

Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Glu Ala Pro Lys Val Ser

420 425 430

Ile His Asp Gln Ile Gln Thr Pro Pro Thr Glu Ser Leu Pro Asn Ser

435 440 445

Glu Arg Asn Leu Asp Ala Ser Glu Thr Ser Val Gln Glu Gly Lys Leu

450 455 460

Leu Thr Lys Gly Val Arg Lys Thr Trp Ser Asp Met Val Asp Glu Glu

465 470 475 480

Glu Gln Gln Leu Gly Asp Asp Lys Pro Trp Ala Asp Met Val Ala Lys

485 490 495

Asp Asp Gln Gln Leu Gly Asp Gly Lys Ser Thr His Gly Val Gly Ile

500 505 510

Thr Glu Gln Asn Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg

515 520 525

Thr Pro Ser Ser Ser Gln Glu Ser Arg Thr His Gln Arg Pro Val Met

530 535 540

Gly Gly Gln Leu Gln Gly Ser Ser Ala Gly Ser Trp Arg Arg Ser Asp

545 550 555 560

Ser Lys Ile Tyr Met Asp Lys Asn Val Asn Trp Asp Leu Val Arg Thr

565 570 575

Ala Pro Thr Trp Ser Arg His Lys Val His Asp His Asn Asn Arg Val

580 585 590

Trp Gln Arg Leu His Thr Ile His Pro Arg Glu Arg Ala Ser Gly Thr

595 600 605

Lys Gln Val Pro Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro

610 615 620

Asp Trp Lys Ser Lys Gly Glu Gly Tyr Gly His Gly Tyr Val Pro Phe

625 630 635 640

Asp Asp Asn Glu His Thr Gln Cys Ser Ser His Ile Glu Ala Ala Thr

645 650 655

His Arg Trp His Asn Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln

660 665 670

Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn

675 680 685

Val Val

690

362

PRT

擬南芥（Arabidopsis thaliana）

Met Glu Gly Lys Phe Ala Ile Ser Glu Ser Thr Asn Leu Leu Gln Arg

1 5 10 15

Ile Lys Asp Phe Thr Gln Ser Val Val Val Asp Leu Ala Glu Gly Arg

20 25 30

Ser Pro Lys Ile Ser Ile Asn Gln Phe Arg Asn Tyr Cys Met Asn Pro

35 40 45

Glu Ala Asp Cys Leu Cys Ser Ser Asp Lys Pro Lys Gly Gln Glu Ile

50 55 60

Phe Thr Leu Lys Lys Glu Pro Gln Thr Tyr Arg Ile Asp Met Leu Leu

65 70 75 80

Arg Val Leu Leu Ile Val Gln Gln Leu Leu Gln Glu Asn Arg His Ala

85 90 95

Ser Lys Arg Asp Ile Tyr Tyr Met His Pro Ser Ala Phe Lys Ala Gln

100 105 110

Ser Ile Val Asp Arg Ala Ile Gly Asp Ile Cys Ile Leu Phe Gln Cys

115 120 125

Ser Arg Tyr Asn Leu Asn Val Val Ser Val Gly Asn Gly Leu Val Met

130 135 140

Gly Trp Leu Lys Phe Arg Glu Ala Gly Arg Lys Phe Asp Cys Leu Asn

145 150 155 160

Ser Leu Asn Thr Ala Tyr Pro Val Pro Val Leu Val Glu Glu Val Glu

165 170 175

Asp Ile Val Ser Leu Ala Glu Tyr Ile Leu Val Val Glu Lys Glu Thr

180 185 190

Val Phe Gln Arg Leu Ala Asn Asp Met Phe Cys Lys Thr Asn Arg Cys

195 200 205

Ile Val Ile Thr Gly Arg Gly Tyr Pro Asp Val Ser Thr Arg Arg Phe

210 215 220

Leu Arg Leu Leu Met Glu Lys Leu His Leu Pro Val His Cys Leu Val

225 230 235 240

Asp Cys Asp Pro Tyr Gly Phe Glu Ile Leu Ala Thr Tyr Arg Phe Gly

245 250 255

Ser Met Gln Met Ala Tyr Asp Ile Glu Ser Leu Arg Ala Pro Asp Met

260 265 270

Lys Trp Leu Gly Ala Phe Pro Ser Asp Ser Glu Val Tyr Ser Val Pro

275 280 285

Lys Gln Cys Leu Leu Pro Leu Thr Glu Glu Asp Lys Lys Arg Thr Glu

290 295 300

Ala Met Leu Leu Arg Cys Tyr Leu Lys Arg Glu Met Pro Gln Trp Arg

305 310 315 320

Leu Glu Leu Glu Thr Met Leu Lys Arg Gly Val Lys Phe Glu Ile Glu

325 330 335

Ala Leu Ser Val His Ser Leu Ser Phe Leu Ser Glu Val Tyr Ile Pro

340 345 350

Ser Lys Ile Arg Arg Glu Val Ser Ser Pro

355 360

383

PRT

擬南芥（Arabidopsis thaliana）

Met Glu Glu Ser Ser Gly Leu Ser Ser Met Lys Phe Phe Ser Asp Gln

1 5 10 15

His Leu Ser Tyr Ala Asp Ile Leu Leu Pro His Glu Ala Arg Ala Arg

20 25 30

Ile Glu Val Ser Val Leu Asn Leu Leu Arg Ile Leu Asn Ser Pro Asp

35 40 45

Pro Ala Ile Ser Asp Leu Ser Leu Ile Asn Arg Lys Arg Ser Asn Ser

50 55 60

Cys Ile Asn Lys Gly Ile Leu Thr Asp Val Ser Tyr Ile Phe Leu Ser

65 70 75 80

Thr Ser Phe Thr Lys Ser Ser Leu Thr Asn Ala Lys Thr Ala Lys Ala

85 90 95

Phe Val Arg Val Trp Lys Val Met Glu Ile Cys Phe Gln Ile Leu Leu

100 105 110

Gln Glu Lys Arg Val Thr Gln Arg Glu Leu Phe Tyr Lys Leu Leu Cys

115 120 125

Asp Ser Pro Asp Tyr Phe Ser Ser Gln Ile Glu Val Asn Arg Ser Val

130 135 140

Gln Asp Val Val Ala Leu Leu Arg Cys Ser Arg Tyr Ser Leu Gly Ile

145 150 155 160

Met Ala Ser Ser Arg Gly Leu Val Ala Gly Arg Leu Phe Leu Gln Glu

165 170 175

Pro Gly Lys Glu Ala Val Asp Cys Ser Ala Cys Gly Ser Ser Gly Phe

180 185 190

Ala Ile Thr Gly Asp Leu Asn Leu Leu Asp Asn Thr Ile Met Arg Thr

195 200 205

Asp Ala Arg Tyr Ile Ile Ile Val Glu Lys His Ala Ile Phe His Arg

210 215 220

Leu Val Glu Asp Arg Val Phe Asn His Ile Pro Cys Val Phe Ile Thr

225 230 235 240

Ala Lys Gly Tyr Pro Asp Ile Ala Thr Arg Phe Phe Leu His Arg Met

245 250 255

Ser Thr Thr Phe Pro Asp Leu Pro Ile Leu Val Leu Val Asp Trp Asn

260 265 270

Pro Ala Gly Leu Ala Ile Leu Cys Thr Phe Lys Phe Gly Ser Ile Gly

275 280 285

Met Gly Leu Glu Ala Tyr Arg Tyr Ala Cys Asn Val Lys Trp Ile Gly

290 295 300

Leu Arg Gly Asp Asp Leu Asn Leu Ile Pro Glu Glu Ser Leu Val Pro

305 310 315 320

Leu Lys Pro Lys Asp Ser Gln Ile Ala Lys Ser Leu Leu Ser Ser Lys

325 330 335

Ile Leu Gln Glu Asn Tyr Ile Glu Glu Leu Ser Leu Met Val Gln Thr

340 345 350

Gly Lys Arg Ala Glu Ile Glu Ala Leu Tyr Cys His Gly Tyr Asn Tyr

355 360 365

Leu Gly Lys Tyr Ile Ala Thr Lys Ile Val Gln Gly Lys Tyr Ile

370 375 380

1330

PRT

擬南芥（Arabidopsis thaliana）

Met Phe Phe Gln His Ser Gln Leu Gln Asn Ser Asp His Leu Leu His

1 5 10 15

Glu Ser Met Ala Asp Ser Asn His Gln Ser Leu Ser Pro Pro Cys Ala

20 25 30

Asn Gly His Arg Ser Thr Ile Ser Leu Arg Asp Asp Gln Gly Gly Thr

35 40 45

Phe Cys Leu Ile Cys Phe Ser Asn Leu Val Ser Asp Pro Arg Ile Pro

50 55 60

Thr Val His Val Ser Tyr Ala Leu His Gln Leu Ser Ile Ala Ile Ser

65 70 75 80

Glu Pro Ile Phe Leu Arg Thr Leu Leu Ser Ser His Ile His Phe Leu

85 90 95

Val Ser Pro Leu Val His Ala Leu Ser Ser Ile Asp Asp Ala Pro Ile

100 105 110

Ala Ile Gln Ile Met Asp Met Ile Ser Leu Leu Cys Ser Val Glu Glu

115 120 125

Ser Ser Ile Gly Glu Asp Phe Val Glu Arg Ile Ser Asp Gln Leu Ser

130 135 140

Ser Gly Ala Leu Gly Trp Ser Arg Arg Gln Leu His Met Leu His Cys

145 150 155 160

Phe Gly Val Leu Met Ser Cys Glu Asn Ile Asp Ile Asn Ser His Ile

165 170 175

Arg Asp Lys Glu Ala Leu Val Cys Gln Leu Val Glu Gly Leu Gln Leu

180 185 190

Pro Ser Glu Glu Ile Arg Gly Glu Ile Leu Phe Ala Leu Tyr Lys Phe

195 200 205

Ser Ala Leu Gln Phe Thr Glu Gln Asn Val Asp Gly Ile Glu Val Leu

210 215 220

Ser Leu Leu Cys Pro Lys Leu Leu Cys Leu Ser Leu Glu Ala Leu Ala

225 230 235 240

Lys Thr Gln Arg Asp Asp Val Arg Leu Asn Cys Val Ala Leu Leu Thr

245 250 255

Ile Leu Ala Gln Gln Gly Leu Leu Ala Asn Ser His Ser Asn Ser Ala

260 265 270

Ser Ser Met Ser Leu Asp Glu Val Asp Asp Asp Pro Met Gln Thr Ala

275 280 285

Glu Asn Val Ala Ala Arg Pro Cys Leu Asn Val Leu Phe Ala Glu Ala

290 295 300

Ile Lys Gly Pro Leu Leu Ser Thr Asp Ser Glu Val Gln Ile Lys Thr

305 310 315 320

Leu Asp Leu Ile Phe His Tyr Ile Ser Gln Glu Ser Thr Pro Ser Lys

325 330 335

Gln Ile Gln Val Met Val Glu Glu Asn Val Ala Asp Tyr Ile Phe Glu

340 345 350

Ile Leu Arg Leu Ser Glu Cys Lys Asp Gln Val Val Asn Ser Cys Leu

355 360 365

Arg Val Leu Asp Leu Phe Ser Leu Ala Glu His Ser Phe Arg Lys Arg

370 375 380

Leu Val Ile Gly Phe Pro Ser Val Ile Arg Val Leu His Tyr Val Gly

385 390 395 400

Glu Val Pro Cys His Pro Phe Gln Ile Gln Thr Leu Lys Leu Ile Ser

405 410 415

Ser Cys Ile Ser Asp Phe Pro Gly Ile Ala Ser Ser Ser Gln Val Gln

420 425 430

Glu Ile Ala Leu Val Leu Lys Lys Met Leu Glu Arg Tyr Tyr Ser Gln

435 440 445

Glu Met Gly Leu Phe Pro Asp Ala Phe Ala Ile Ile Cys Ser Val Phe

450 455 460

Val Ser Leu Met Lys Thr Pro Ser Phe Gly Glu Thr Ala Asp Val Leu

465 470 475 480

Thr Ser Leu Gln Glu Ser Leu Arg His Ser Ile Leu Ala Ser Leu Ser

485 490 495

Leu Pro Glu Lys Asp Ser Thr Gln Ile Leu His Ala Val Tyr Leu Leu

500 505 510

Asn Glu Ile Tyr Val Tyr Cys Thr Ala Ser Thr Ser Ile Asn Met Thr

515 520 525

Ser Cys Ile Glu Leu Arg His Cys Val Ile Asp Val Cys Thr Ser His

530 535 540

Leu Leu Pro Trp Phe Leu Ser Asp Val Asn Glu Val Asn Glu Glu Ala

545 550 555 560

Thr Leu Gly Ile Met Glu Thr Phe His Ser Ile Leu Leu Gln Asn Ser

565 570 575

Asp Ile Gln Ala Lys Glu Phe Ala Glu Leu Leu Val Ser Ala Asp Trp

580 585 590

Phe Ser Phe Ser Phe Gly Cys Leu Gly Asn Phe Cys Thr Asp Asn Met

595 600 605

Lys Gln Arg Ile Tyr Leu Met Leu Ser Ser Leu Val Asp Ile Leu Leu

610 615 620

Glu Gln Lys Thr Gly Ser His Ile Arg Asp Ala Leu His Cys Leu Pro

625 630 635 640

Ser Asp Pro Gln Asp Leu Leu Phe Leu Leu Gly Gln Ala Ser Ser Asn

645 650 655

Asn Gln Glu Leu Ala Ser Cys Gln Ser Ala Ala Leu Leu Ile Phe His

660 665 670

Thr Ser Ser Ile Tyr Asn Asp Arg Leu Ala Asp Asp Lys Leu Val Leu

675 680 685

Ala Ser Leu Glu Gln Tyr Ile Ile Leu Asn Lys Thr Ser Leu Ile Cys

690 695 700

Ala Ile Ser Asp Ser Pro Ala Leu Leu Asn Leu Val Asn Leu Tyr Gly

705 710 715 720

Leu Cys Arg Ser Leu Gln Asn Glu Arg Tyr Gln Ile Ser Tyr Ser Leu

725 730 735

Glu Ala Glu Arg Ile Ile Phe His Leu Leu Asn Glu Tyr Glu Trp Asp

740 745 750

Leu Gly Ser Ile Asn Ile His Leu Glu Ser Leu Lys Trp Leu Phe Gln

755 760 765

Gln Glu Ser Ile Ser Lys Ser Leu Ile Tyr Gln Ile Gln Lys Ile Ser

770 775 780

Arg Asn Asn Leu Ile Gly Asn Glu Val His Asn Val Tyr Gly Asp Gly

785 790 795 800

Arg Gln Arg Ser Leu Thr Tyr Trp Phe Ala Lys Leu Ile Ser Glu Gly

805 810 815

Asp Asn Tyr Ala Ala Thr Leu Leu Val Asn Leu Leu Thr Gln Leu Ala

820 825 830

Glu Lys Glu Glu Gln Glu Asn Asp Val Thr Ser Ile Leu Asn Leu Met

835 840 845

Asn Thr Ile Val Ser Ile Phe Pro Thr Ala Ser Asn Asn Leu Ser Met

850 855 860

Asn Gly Ile Gly Ser Val Val His Arg Leu Val Ser Gly Phe Ser Asn

865 870 875 880

Ser Ser Leu Gly Thr Ser Phe Lys Thr Leu Leu Leu Leu Val Phe Asn

885 890 895

Ile Leu Thr Ser Val Gln Pro Ala Val Leu Met Ile Asp Glu Ser Trp

900 905 910

Tyr Ala Val Ser Ile Lys Leu Leu Asn Phe Leu Ser Leu Arg Asp Thr

915 920 925

Ala Ile Lys Gln Asn His Glu Asp Met Val Val Ile Gly Ile Leu Ser

930 935 940

Leu Val Leu Tyr His Ser Ser Asp Gly Ala Leu Val Glu Ala Ser Arg

945 950 955 960

Asn Ile Val Ser Asn Ser Tyr Leu Val Ser Ala Ile Asn Thr Val Val

965 970 975

Asp Val Ala Cys Ser Lys Gly Pro Ala Leu Thr Gln Cys Gln Asp Glu

980 985 990

Thr Asn Ile Gly Glu Ala Leu Ala Phe Thr Leu Leu Leu Tyr Phe Phe

995 1000 1005

Ser Leu Arg Ser Leu Gln Ile Val Leu Ala Gly Ala Val Asp Trp

1010 1015 1020

Gln Ala Phe Phe Gly Thr Ser Thr Ser Leu Glu Thr Leu Pro Val

1025 1030 1035

Val Cys Ile Tyr Cys His Asn Leu Cys Arg Leu Met His Phe Gly

1040 1045 1050

Ala Pro Gln Ile Lys Leu Ile Ala Ser Tyr Cys Leu Leu Glu Leu

1055 1060 1065

Leu Thr Gly Leu Ser Glu Gln Val Asp Ile Lys Lys Glu Gln Leu

1070 1075 1080

Gln Cys Ser Ser Ser Tyr Leu Lys Ser Met Lys Ala Val Leu Gly

1085 1090 1095

Gly Leu Val Phe Cys Asp Asp Ile Arg Val Ala Thr Asn Ser Ala

1100 1105 1110

Leu Cys Leu Ser Met Ile Leu Gly Trp Glu Asp Met Glu Gly Arg

1115 1120 1125

Thr Glu Met Leu Lys Thr Ser Ser Trp Tyr Arg Phe Ile Ala Glu

1130 1135 1140

Glu Met Ser Val Ser Leu Ala Leu Pro Cys Ser Ala Ser Ser Thr

1145 1150 1155

Tyr Val Asn His His Lys Pro Ala Val Tyr Leu Thr Val Ala Met

1160 1165 1170

Leu Arg Leu Lys Asn Lys Pro Val Trp Leu Arg Thr Val Phe Asp

1175 1180 1185

Glu Ser Cys Ile Ser Ser Met Ile Gln Asn Leu Asn Gly Ile Asn

1190 1195 1200

Ile Ser Arg Glu Ile Val Ile Leu Phe Arg Glu Leu Met Gln Ala

1205 1210 1215

Glu Leu Leu Asn Ser Gln Gln Val Thr Lys Leu Asp Arg Ala Phe

1220 1225 1230

Gln Glu Cys Arg Lys Gln Met His Arg Asn Gly Thr Arg Asp Glu

1235 1240 1245

Thr Val Glu Glu Gln Val Gln Arg Lys Ile Pro Ser Ile His Asp

1250 1255 1260

His Ser Glu Phe Cys Asn Tyr Leu Val His Leu Met Val Ser Asn

1265 1270 1275

Ser Phe Gly His Pro Ser Glu Ser Glu Thr Tyr Thr Gln Lys Lys

1280 1285 1290

Lys Gln Ile Leu Asp Glu Met Glu Gln Leu Ser Glu Leu Ile Ser

1295 1300 1305

Thr Arg Glu Gly Arg Val Ser Pro Ile Gln Glu Glu Thr Arg Gln

1310 1315 1320

Met Gln Thr Glu Arg Ile Val

1325 1330

385

PRT

擬南芥（Arabidopsis thaliana）

Met Ser Ser Ser Val Ala Glu Ala Asn His Thr Glu Lys Glu Glu Ser

1 5 10 15

Leu Arg Leu Ala Ile Ala Val Ser Leu Leu Arg Ser Lys Phe Gln Asn

20 25 30

His Gln Ser Ser Ser Ser Thr Ser Arg Cys Tyr Val Ser Ser Glu Ser

35 40 45

Asp Ala Leu Arg Trp Lys Gln Lys Ala Lys Glu Arg Lys Lys Glu Ile

50 55 60

Ile Arg Leu Gln Glu Asp Leu Lys Asp Ala Glu Ser Ser Phe His Arg

65 70 75 80

Asp Leu Phe Pro Ala Asn Ala Ser Cys Lys Cys Tyr Phe Phe Asp Asn

85 90 95

Leu Gly Val Phe Ser Gly Arg Arg Ile Gly Glu Ala Ser Glu Ser Arg

100 105 110

Phe Asn Asp Val Leu Arg Arg Arg Phe Leu Arg Leu Ala Cys Val Val

115 120 125

Ile Leu Ser Leu Ala Arg Arg Arg Ser Arg Arg Lys Leu Thr Arg Ser

130 135 140

Ser Gln Arg Leu Gln Pro Ser Glu Pro Asp Tyr Glu Glu Glu Ala Glu

145 150 155 160

His Leu Arg Ile Ser Ile Asp Phe Leu Leu Glu Leu Ser Glu Ala Asp

165 170 175

Ser Asn Asp Ser Asn Phe Ser Asn Trp Ser His Gln Ala Val Asp Phe

180 185 190

Ile Phe Ala Ser Leu Lys Lys Leu Ile Ser Met Gly Arg Asn Leu Glu

195 200 205

Ser Val Glu Glu Ser Ile Ser Phe Met Ile Thr Gln Leu Ile Thr Arg

210 215 220

Met Cys Thr Pro Val Lys Gly Asn Glu Val Lys Gln Leu Glu Thr Ser

225 230 235 240

Val Gly Phe Tyr Val Gln His Leu Ile Arg Lys Leu Gly Ser Glu Pro

245 250 255

Phe Ile Gly Gln Arg Ala Ile Phe Ala Ile Ser Gln Arg Ile Ser Ile

260 265 270

Leu Ala Glu Asn Leu Leu Phe Met Asp Pro Phe Asp Glu Ser Phe Pro

275 280 285

Glu Met Asp Glu Cys Met Phe Ile Leu Ile Gln Leu Ile Glu Phe Leu

290 295 300

Ile Cys Asp Tyr Leu Leu Pro Trp Ala Asn Glu Ala Phe Asp Asn Val

305 310 315 320

Met Phe Glu Glu Trp Ile Ala Ser Val Val His Ala Arg Lys Ala Val

325 330 335

Lys Ala Leu Glu Glu Arg Asn Gly Leu Tyr Leu Leu Tyr Met Asp Arg

340 345 350

Val Thr Gly Glu Leu Ala Lys Arg Val Gly Gln Ile Thr Ser Phe Arg

355 360 365

Glu Val Glu Pro Ala Ile Leu Asp Lys Ile Leu Ala Tyr Gln Glu Ile

370 375 380

Glu

385

210> 33

449

PRT

擬南芥（Arabidopsis thaliana）

Met Lys Met Asn Ile Asn Lys Ala Cys Asp Leu Lys Ser Ile Ser Val

1 5 10 15

Phe Pro Pro Asn Leu Arg Arg Ser Ala Glu Pro Gln Ala Ser Gln Gln

20 25 30

Leu Arg Ser Gln Gln Ser Gln Gln Ser Phe Ser Gln Gly Pro Ser Ser

35 40 45

Ser Gln Arg Gly Cys Gly Gly Phe Ser Gln Met Thr Gln Ser Ser Ile

50 55 60

Asp Glu Leu Leu Ile Asn Asp Gln Arg Phe Ser Ser Gln Glu Arg Asp

65 70 75 80

Leu Ser Leu Lys Lys Val Ser Ser Cys Leu Pro Pro Ile Asn His Lys

85 90 95

Arg Glu Asp Ser Gln Leu Val Ala Ser Arg Ser Ser Ser Gly Leu Ser

100 105 110

Arg Arg Trp Ser Ser Ala Ser Ile Gly Glu Ser Lys Ser Gln Ile Ser

115 120 125

Glu Glu Leu Glu Gln Arg Phe Gly Met Met Glu Thr Ser Leu Ser Arg

130 135 140

Phe Gly Met Met Leu Asp Ser Ile Gln Ser Asp Ile Met Gln Ala Asn

145 150 155 160

Arg Gly Thr Lys Glu Val Phe Leu Glu Thr Glu Arg Ile Gln Gln Lys

165 170 175

Leu Thr Leu Gln Asp Thr Ser Leu Gln Gln Leu Arg Lys Glu Gln Ala

180 185 190

Asp Ser Lys Ala Ser Leu Asp Gly Gly Val Lys Phe Ile Leu Glu Glu

195 200 205

Phe Ser Lys Asp Pro Asn Gln Glu Lys Leu Gln Lys Ile Leu Gln Met

210 215 220

Leu Thr Thr Ile Pro Glu Gln Val Glu Thr Ala Leu Gln Lys Ile Gln

225 230 235 240

Arg Glu Ile Cys His Thr Phe Thr Arg Glu Ile Gln Val Leu Ala Ser

245 250 255

Leu Arg Thr Pro Glu Pro Arg Val Arg Val Pro Thr Ala Pro Gln Val

260 265 270

Lys Ala Lys Glu Asn Leu Pro Glu Gln Arg Gly Gln Ala Ala Lys Val

275 280 285

Leu Thr Ser Leu Lys Met Pro Glu Pro Arg Val Gln Val Pro Ala Ala

290 295 300

Pro Gln Ala Lys Glu Asn Phe Pro Glu Gln Arg Gly Pro Val Ala Lys

305 310 315 320

Ser Asn Ser Phe Cys Asn Thr Thr Leu Lys Thr Lys Gln Pro Gln Phe

325 330 335

Pro Arg Asn Pro Asn Asp Ala Ser Ala Arg Ala Val Lys Pro Tyr Leu

340 345 350

Ser Pro Lys Ile Gln Val Gly Cys Trp Lys Thr Val Lys Pro Glu Lys

355 360 365

Ser Asn Phe Lys Lys Arg Ala Thr Arg Lys Pro Val Lys Ser Glu Ser

370 375 380

Thr Arg Thr Gln Phe Glu Gln Cys Ser Val Val Ile Asp Ser Asp Glu

385 390 395 400

Glu Asp Ile Asp Gly Gly Phe Ser Cys Leu Ile Asn Glu Asn Thr Arg

405 410 415

Gly Thr Asn Phe Glu Trp Asp Ala Glu Lys Glu Thr Glu Arg Ile Leu

420 425 430

Arg Thr Ala Arg Arg Thr Lys Arg Lys Phe Gly Asn Pro Ile Ile Ile

435 440 445

Asn

233

PRT

擬南芥（Arabidopsis thaliana）

Met Arg His Asn Ile Lys Phe Lys Ser Lys Gly Thr Leu Lys Ile Arg

1 5 10 15

Asn Thr Ala Gln Ile Ser Leu Trp Lys Lys Cys Ser Asp Ser Met Ile

20 25 30

Ala Asp Gln Thr Tyr Leu Phe Ile Asn Arg Val Gln Asp Arg Arg Phe

35 40 45

Asp Glu Glu Ser Leu Arg Ile Leu Glu Leu Ser Leu Val Ala Met Asn

50 55 60

Val Lys Ser Phe Leu Glu Val Arg Ser Arg Leu Arg Asp Phe Met Arg

65 70 75 80

Ser Glu Ser Val Val Ile Phe Gly Glu Leu Thr Gly Glu Ser Met Val

85 90 95

Ala Lys Leu Ser Val Leu Glu Phe Phe Ala Arg Ala Phe Ala Leu Leu

100 105 110

Gly Asp Met Glu Ser Cys Leu Ala Met Arg Tyr Glu Ala Leu Asn Leu

115 120 125

Arg Gln Leu Lys Ser Pro Ser Cys Leu Trp Leu Gly Val Ser His Ser

130 135 140

Glu Trp Thr Lys Phe Ala Val Gln Ser Met Glu Asn Gly Phe Pro Ser

145 150 155 160

Ile Ala Gly Lys Ala Ser Glu Asn Ala Leu Leu Ser Leu Lys Lys Asp

165 170 175

Ser Leu Ile Glu Pro Lys Ser Glu Asp Asn Ser Asp Ile Leu Asp Ala

180 185 190

Ala Glu Lys Val Arg Arg Leu Arg Asp Ser Ala Ala Ser Leu Thr Ser

195 200 205

Ser His Ser Gly Ile Phe Ile Tyr Ile Val Ser Ser Leu Lys Phe Ala

210 215 220

Val Cys Asn Arg Leu Leu Thr Thr Phe

225 230

617

PRT

擬南芥（Arabidopsis thaliana）

Met Phe Tyr Ser His Gln Leu Leu Ala Arg Lys Ala Pro Leu Gly Gln

1 5 10 15

Ile Trp Met Ala Ala Thr Leu His Ala Lys Ile Asn Arg Lys Lys Leu

20 25 30

Asp Lys Leu Asp Ile Ile Gln Ile Cys Glu Glu Ile Leu Asn Pro Ser

35 40 45

Val Pro Met Ala Leu Arg Leu Ser Gly Ile Leu Met Gly Gly Val Val

50 55 60

Ile Val Tyr Glu Arg Lys Val Lys Leu Leu Phe Asp Asp Val Asn Arg

65 70 75 80

Phe Leu Val Glu Ile Asn Gly Ala Trp Arg Thr Lys Ser Val Pro Asp

85 90 95

Pro Thr Leu Leu Pro Lys Gly Lys Thr His Ala Arg Lys Glu Ala Val

100 105 110

Thr Leu Pro Glu Asn Glu Glu Ala Asp Phe Gly Asp Phe Glu Gln Thr

115 120 125

Arg Asn Val Pro Lys Phe Gly Asn Tyr Met Asp Phe Gln Gln Thr Phe

130 135 140

Ile Ser Met Arg Leu Asp Glu Ser His Val Asn Asn Asn Pro Glu Pro

145 150 155 160

Glu Asp Leu Gly Gln Gln Phe His Gln Ala Asp Ala Glu Asn Ile Thr

165 170 175

Leu Phe Glu Tyr His Gly Ser Phe Gln Thr Asn Asn Glu Thr Tyr Asp

180 185 190

Arg Phe Glu Arg Phe Asp Ile Glu Gly Asp Asp Glu Thr Gln Met Asn

195 200 205

Ser Asn Pro Arg Glu Gly Ala Glu Ile Pro Thr Thr Leu Ile Pro Ser

210 215 220

Pro Pro Arg His His Asp Ile Pro Glu Gly Val Asn Pro Thr Ser Pro

225 230 235 240

Gln Arg Gln Glu Gln Gln Glu Asn Arg Arg Asp Gly Phe Ala Glu Gln

245 250 255

Met Glu Glu Gln Asn Ile Pro Asp Lys Glu Glu His Asp Arg Pro Gln

260 265 270

Pro Ala Lys Lys Arg Ala Arg Lys Thr Ala Thr Ser Ala Met Asp Tyr

275 280 285

Glu Gln Thr Ile Ile Ala Gly His Val Tyr Gln Ser Trp Leu Gln Asp

290 295 300

Thr Ser Asp Ile Leu Cys Arg Gly Glu Lys Arg Lys Val Arg Gly Thr

305 310 315 320

Ile Arg Pro Asp Met Glu Ser Phe Lys Arg Ala Asn Met Pro Pro Thr

325 330 335

Gln Leu Phe Glu Lys Asp Ser Ser Tyr Pro Pro Gln Leu Tyr Gln Leu

340 345 350

Trp Ser Lys Asn Thr Gln Val Leu Gln Thr Ser Ser Ser Glu Ser Arg

355 360 365

His Pro Asp Leu Arg Ala Glu Gln Ser Pro Gly Phe Val Gln Glu Arg

370 375 380

Met His Asn His His Gln Thr Asp His His Glu Arg Ser Asp Thr Ser

385 390 395 400

Ser Gln Asn Leu Asp Ser Pro Ala Glu Ile Leu Arg Thr Val Arg Thr

405 410 415

Gly Lys Gly Ala Ser Val Glu Ser Met Met Ala Gly Ser Arg Ala Ser

420 425 430

Pro Glu Thr Ile Asn Arg Gln Ala Ala Asp Ile Asn Val Thr Pro Phe

435 440 445

Tyr Ser Gly Asp Asp Val Arg Ser Met Pro Ser Thr Pro Ser Ala Arg

450 455 460

Gly Ala Ala Ser Ile Asn Asn Ile Glu Ile Ser Ser Lys Ser Arg Met

465 470 475 480

Pro Asn Arg Lys Arg Pro Asn Ser Ser Pro Arg Arg Gly Leu Glu Pro

485 490 495

Val Ala Glu Glu Arg Pro Trp Glu His Arg Glu Tyr Glu Phe Glu Phe

500 505 510

Ser Met Leu Pro Glu Lys Arg Phe Thr Ala Asp Lys Glu Ile Leu Phe

515 520 525

Glu Thr Ala Ser Thr Gln Thr Gln Lys Pro Val Cys Asn Gln Ser Asp

530 535 540

Glu Met Ile Thr Asp Ser Ile Lys Ser His Leu Lys Thr His Phe Glu

545 550 555 560

Thr Pro Gly Ala Pro Gln Val Glu Ser Leu Asn Lys Leu Ala Val Gly

565 570 575

Met Asp Arg Asn Ala Ala Ala Lys Leu Phe Phe Gln Ser Cys Val Leu

580 585 590

Ala Thr Arg Gly Val Ile Lys Val Asn Gln Ala Glu Pro Tyr Gly Asp

595 600 605

Ile Leu Ile Ala Arg Gly Pro Asn Met

610 615

DNA

人工序列

合成引物

gacatcggca cttgcttaga g 21

DNA

人工序列

合成引物

gcgatatagc tcccactggt t 21

DNA

人工序列

合成引物

gagtttacta tactctgccg ccggcgagaa c 31

DNA

人工序列

合成引物

ctccacctgg agtttactat gccccgccgc cggcgaga 38

DNA

人工序列

合成引物

ccacctggag ttgcgtatac tccgccgcgg cg 32

DNA

人工序列

合成引物

ccacctggag ttgcgagaac aagtgatcat gtggc 35

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