奧林巴斯BX53報價和功能

2023-12-10 03:32:21 1

　　傳統單點掃描與轉盤式掃描的區別：

工程師 張帆 13810005848

    轉盤式是多點同步掃描，轉盤上分布有20000個螺旋排列的針孔，而雷射光源覆蓋約2000個針孔的範圍(即掃描區域)，藉助轉盤的轉動，實現對樣品的完整掃描。相當於2000個雷射點同步照射樣品，同步激發，CCD上同步採集，所以掃描速度非常的快。轉盤式是目前活細胞共聚焦非常先進的技術，最大的特點是速度快，靈敏度高，光漂白和光毒性水平低，最適合活細胞、快速、6D掃描研究。

    　　Yokogawa雙轉盤式共聚焦掃描技術

　　Yokogawa的CV1000轉盤式共聚焦掃描成像系統是定位於長時間、多時相、6D活細胞成像的一體化共聚焦系統，它整合了顯微鏡，共聚焦光路系統，以及活細胞工作站等諸多模塊於一體，不需暗室操作並且集成了溫度、溼度控制等繁瑣過程。整套安裝簡易，操作簡單，使用方便，並且廠家攜代理商提供完善的售後服務。

　　CV1000使用的雙轉盤共聚焦掃描頭，是活細胞成像的金標準，最大限度地降低了光毒性和光漂白。CV1000是目前唯一的能長時間對胚胎幹細胞做3D成像，然後被觀察的胚胎幹細胞能順利發育為成體小鼠的系統。該系統的精確的溫度控制系統(精確度可達0.1℃)，使得幹細胞，胚胎，以及其他細胞可在穩定的溫度環境中生長，精確地完成您的實驗操作。為活細胞的觀察在技術水平上提供了雙保險，使得CV1000更適合於非常適合於精確度要求極高的生命過程的觀察，如發育生物學，幹細胞研究等領域的長時高速6D成像，尤其適合於幹細胞增殖和分化，iPS細胞成像，胚胎發育，轉基因細胞實時觀察等領域。

　　Software for Image Analysis

　　Voxx : Written by Jeff Clendenon and Jason Byars

　　A voxel-based (not surface-based) 3D imaging program capable of near real-time rendering of large microscopy data sets on inexpensive personal computers has been developed in the Indiana Center for Biological Microscopy. It was designed to use the low-cost (NVIDIA GeForce) 3D graphics processors found on video boards in many lab and office PCs, instead of the more expensive voxel processors or graphics workstations commonly used by others for voxel-based rendering. The program currently runs on Windows 98/NT/2000, with Apple MacOS and Linux ports underway.

　　Confocal Assistant (ver 4.02): Written by Todd Clark Brelje

　　A very neat and powerful program that reads Bio-Rad PIC images. It allows users to make format conversion, projection, movies, color encoding, montages, etc. Those who use Metamorph (Universal Imaging) will still find Confocal Assistant useful. It is a lot faster than Metamorph when you make projections. Best of all, it''s free!

　　ImageJ : From the NIH

　　A free image processing tool from the NIH. It has an extesive library of plugins.

　　Zeiss LSMoffline browser R3.5: From Carl Zeiss, Inc.

　　You need it to convert your LSM images to Tiff format. If you have problem installing and have DAO related error messages, you may need the dao.zip fil

　　LCSlite：From Leica

　　ImageTool

　　Confocal Assistant

　　Version 4.02

　　An amazing little program written by Todd Clark Brelje for reviewing and manipulating BioRad .pic image stacks

　　Zeiss LSM Image Examiner

　　Version 3.5

　　From Carl Zeiss, Inc. You will need it for browsing your Zeiss libraries away from the microscope.