分泌型衣原體多肽、其編碼多核苷酸及其治療和診斷用途的製作方法

2023-10-10 22:51:29 2

專利名稱：分泌型衣原體多肽、其編碼多核苷酸及其治療和診斷用途的製作方法
技術領域：
本發明涉及分泌型衣原體多肽(secreted Chlamydia polypeptides)。更具體地，本發明涉及由革蘭氏陰性細菌菌株表達的並由所述菌株的III型分泌途徑分泌的分泌型衣原體多肽。本發明還涉及編碼這些分泌型衣原體多肽的多核苷酸，並涉及這些分泌型衣原體多肽的治療用途(包括免疫接種)和診斷用途。
背景技術：
衣原體(Chlamydia)是僅能在真核宿主細胞內增殖的革蘭氏陰性細菌。三種人類致病性類型，即沙眼衣原體(Chlamydia trachomatis)、鸚鵡熱衣原體(Chlamydia psittaci)和肺炎衣原體(Chlamydia pneumoniae)，可引起多種疾病，包括沙眼、性傳播疾病、盆腔炎症性疾病、呼吸道疾病例如支氣管炎、肺炎及其後遺症(Gregory，D.W.and W.Schaffner.(1997).Psittacosis.Seminars in Respiratory Infections.127-11；Kuo，C.-C.，L.Jackson，L.Campbell，and J.Grayston.(1995).Chlamydia pneumoniae(TWAR).Clin.Microbiol.Rev.8451-61；Stamm，W.E.(1999).Chlamydiatrachomatis infectionsprogress and problems.J.Infect.Dis.179S380-3)。此外，已經證實鸚鵡熱衣原體和貓心衣原體(Chlamydia pecorum)對非人類動物具有致病性，因此對農業經濟具有重要的影響。具體而言，已經發現鸚鵡熱衣原體和貓心衣原體與牛、豬和羊的流產和肺炎有因果關聯(Fukushi等，(1993).Microbiol Immunol.37(7)516-522；Tanner等，(1999).ChlamydiaIntercellular Biology，Pathogenesis，and Immunity.Chapter 1)。
在其周期中，衣原體具有兩種截然不同的形態一種小的感染性形式，即原體(elementary body)(EB，直徑0.3μm)，和一種較大的複製型形式，即網狀體(reticulate body)(RB，直徑1μm)(Moulder，J.W.(1991).Interaction of chlamydiae and host cells in vitro.Microbiol.Rev.55143-90.)。EB是用於在細胞外間隙存活所採取的形式其外膜通過網狀的二硫鍵而變得堅硬，細菌的代謝不活躍。它們通過附著於合適的宿主細胞(主要為黏膜的上皮細胞)而啟動感染，並通過一種類似於吞噬作用的機制被內化(internalized)(Boleti，H.，A.Benmerah，D.Ojcius，N.Cerf-Bensussan，and A.Dautry-Varsat.(1999).Chlamydia infection of epithelialcells expressing dynamin and Eps 15 mutantsclathrin-independent entry intocells and dynamin-dependent productive growth.J.Cell.Sci.1121487-1496)。內化後僅僅數小時，EB即變為複製型的RB，後者在一種生長液泡(growing vacuole)增殖並產生多達大約一千個子代。感染後2至3天，RB變回EB，後者被輸送到細胞外間隙，並能夠開始新的感染周期。
在宿主細胞內的整個周期中，衣原體存在於一種稱為包涵體的膜結合型腔室結構(membrane-bound compartment)內。通過防止包涵體與宿主細胞的酸性降解腔室結構發生融合，細菌可逃脫宿主的防禦機制(即細胞漿蛋白酶或溶酶體酶)。此外，由於絕大多數細菌抗原並不到達被感染的細胞的質膜，因此這些抗原不會被宿主的免疫系統發現。對吞噬溶酶體融合的抑制僅限於載有鸚鵡熱衣原體的液泡(Eissenberg，L.G.，andP.B.Wyrick.(1981)Infect.Immun.32889-896；Friis，R.R.(1972).Interaction of L cells and Chlamydia psittacientry of the parasite and hostresponses to its development.J.Bacteriol.110706-721；Heinzen，R.A.，M.A.Scidmore，D.D.Rockey，and T.Hackstadt.(1996).Differentialinteraction with endocytic and exocytic pathways distinguish parasitophorousvacuoles of Coxiella burnetii and Chlamydia trachomatis.Infect.Immun.64769-809；Schramm，N.，C.R.Bagnell，and P.B.Wyrick.(1996).Vesiclescontaining Chlamydia trachomatis serovar L2 remain above pH 6 withinHEC-1B cells.Infect.Immun.641208-1214)。實際上，衣原體似乎僅與內吞性腔室結構發生極少的交換(Taraska，T.，D.M.Ward，R.S.Ajioka，P.B.Wyrick，S.R.Davis-Kaplan，C.H.Davis，and J.Kaplan.(1996).The latechlamydial inclusion membrane is not derived from the endocytic pathwayand is relatively deficient in host proteins.Infect.Immun.643713-372；VanOoij，C.，G.Apodaca，and J.Engel.(1997).Characterization of the Chlamydiatrachomatis vacuole and its interaction with the host endocytic pathway inHeLa cells.Infect.Immun.65758-766)。
不過，在其發展周期中，包涵體的體積顯著增大，直至其佔據細胞漿的一大部分。這種生長所必需的部分脂質來自於宿主細胞(Hatch，G.M.，and G.McClarty.(1998).Phospholipid composition of purifiedChlamydia trachomatis mimics that of the eucaryotic host cell.Infection Immunity.663727-35)。在高爾基體中合成的神經磷脂酶被轉移至包涵體膜並摻入細菌內部(Hackstadt，T.，M.A.Scidmore，and D.D.Rockey.(1995).Lipid metabolism in Chlamydia trachomatis-infected cellsdirectedtrafficking of Golgi-derived sphingolipids to the chlamydial inclusion.Proc.Natl.Acad.Sci，USA.924877-4881)。
令人驚異地，尚未發現與包涵體相關的來源於真核細胞的蛋白。不過，通過使用針對多種衣原體蛋白產生的抗體，採用螢光顯微鏡，已經在包涵體膜上定位了多種原核細胞蛋白(Bannantine，J.P.，R.S.Griffiths，W.Viratyosin，W.J.Brown，and D.D.Rockey.(2000).A secondary structuremotif predictive of protein localization to the chlamydial inclusion membrane.Cell.Microbiol.235-47；Bannantine，J.P.，D.D.Rockey，and T.Hackstadt.(1998).Tandem genes of Chlamydia psittaci that encode proteins located tothe inclusion membrane.Mol.Microbiol.281017-26；Rockey，D.D.，R.A.Heinzen，and T.Hackstadt.(1995).Cloning and characterization of aChlamydia psittaci gene coding for a protein localized in the inclusionmembrane of infected cells.Mol.Microbiol.15617-626；Scidmore-Carlson，M.A.，E.I.Shaw，C.A.Dooley，E.R.Fischer，and T.Hackstadt.(1999).Identification and characterization of a Chlamydia trachomatis early operonencoding four novel inclusion membrane proteins.Mol.Microbiol.33753-765)。這些蛋白不具有序列相似性，但均具有一種大的疏水性區域(40至70個胺基酸)，並因此被歸入一種被稱為Inc家族的結構家族。
最早鑑定的三個Inc蛋白，即IncA、IncB和IncC，很可能是包涵體的主要成分，這是因為其可被來自處於恢復期的感染了鸚鵡熱衣原體的豚鼠的抗血清所識別(Bannantine，J.，W.Stamm，R.Suchland，and D.Rockey.(1998).Chlamydia trachomatis IncA is localized to the inclusionmembrane and is recognized by antisera from infected humans and primates.Infect.Immun.666017-6021；Rockey，D.D.，R.A.Heinzen，and T.Hackstadt.(1995).Cloning and characterization of a Chlamydia psittaci genecoding for a protein localized in the inclusion membrane of infected cells.Mol.Microbiol.15617-626)。它們具有由沙眼衣原體和肺炎衣原體基因組編碼的同源物，具有大約20％的總體序列相同性，而在相似性最高的區域具有高達50％的序列相同性。
基於兩個標準，將Inc蛋白歸類為一個家族它們具有一個大疏水性結構域(大於40個殘基)，並且它們定位於宿主細胞內的包涵體膜。由沙眼衣原體基因組編碼的幾種蛋白質與來自肺炎衣原體的一種蛋白質屬於這一家族，這是通過使用特異性抗體以免疫螢光技術將它們定位於包涵體膜而證實的(Bannantine，J.，W.Stamm，R.Suchland，and D.Rockey.(1998).Chlamydia trachomatis IncA is localized to the inclusion membraneand is recognized by antisera from infected humans and primates.Infect.Immun.666017-6021；Bannantine，J.P.，R.S.Griffiths，W.Viratyosin，W.J.Brown，and D.D.Rockey.(2000).A secondary structure motif predictive ofprotein localization to the chlamydial inclusion membrane.Cell.Microbiol.235-47；Bannantine，J.P.，D.D.Rockey，and T.Hackstadt.(1998).Tandemgenes of Chlamydia psittaci that encode proteins located to the inclusionmembrane.Mol.Microbiol.281017-26；Rockey，D.D.，R.A.Heinzen，andT.Hackstadt.(1995).Cloning and characterization of a Chlamydia psittacigene coding for a protein localized in the inclusion membrane of infectedcells.Mol.Microbiol.15617-626.；Scidmore-Carlson，M.A.，E.I.Shaw，C.A.Dooley，E.R.Fischer，and T.Hackstadt.(1999).Identification andcharacterization of a Chlamydia trachomatis early operon encoding fournovel inclusion membrane proteins.Mol.Microbiol.33753-765)。不過，由於缺乏研究衣原體菌種成員的遺傳學工具，迄今對它們如何被分泌並插入包涵體膜的機制還沒有進行研究。
衣原體蛋白是如何到達包涵體膜的呢？目前已經描述了革蘭氏陰性細菌的4種蛋白質分泌途徑(I至IV型)。III型分泌機制能夠在細菌與細胞表面的接觸過程中使得細菌效應物(effectors)轉運進入真核細胞的細胞漿(Hueck，C.(1998).Type III protein secretion systems in bacterialpathogens of animals and plants.Microbiol.Mol.Biol.Rev.62379-433)。本發明人推測衣原體採用III型分泌將蛋白質插入包涵體膜(Subtil A.，A.Blocker，and A.Dautry-Varsat.(2000).Type III secretion system inChlamydiaidentified members and candidates.Microbes Infect.2367-369)。衣原體的基因組中存在編碼推定的III型分泌機製成分的基因提示Inc蛋白可能是由III型分泌途徑分泌的，這是因為III型分泌被大量革蘭氏陰性病原體採用，用來將細菌蛋白質插入真核細胞膜內部或轉運通過真核細胞膜。由於缺乏操控衣原體基因組的遺傳學工具，因此直接驗證這一假設存在困難。志賀氏菌屬(Shigella)採用III型分泌系統以進入上皮細胞並播散(Nhieu，G.T.，and P.J.Sansonetti.(1999).Mechanism ofShigella entry into epithelial cells.Curr.Opin.Microbiol.251-5；Page，A.-L.，H.Ohayon，P.J.Sansonetti，and C.Parsot.(1999).The secreted IpaB andIpaC invasins and their cytoplasmic chaperone IpgC are required forintercellular dissemination of Shigella flexneri.Cell.Microbiol.1183-193；Schuch，R.，R.C.Sandlin，and A.T.Maurelli.(1999).A system for identifyingpost-invasion functions of invasion genesrequirements for the Mxi-Spa typeIII secretion pathway of Shigella flexneri in intercellular dissemination，Mol.Microbiol.34675-689)。通過III型分泌途徑分泌蛋白質所涉及的分泌信號還不知道。不過，其通常定位於編碼被分泌的蛋白質的mRNA的前15個密碼子內，或者是定位於被分泌的蛋白質的N-端部分(Anderson，D.M.，and O.Schneewind.(1999).Type III machines of Gram-negativepathogensinjecting virulence factors into host cells and more.Curr.Opin.Microbiol.218-24)。儘管一些衣原體菌種的基因組已經是已知的，但仍無法基於它們的胺基酸序列而鑑別被分泌的蛋白質。
志賀氏菌屬中編碼III型分泌系統的基因是已知的(Hueck，C.(1998).Type III protein secretion systems in bacterial pathogens of animals andplants.Microbiol.Mol.Biol.Rev.62379-433)，並且目前推測Inc蛋白可能是通過類似機制被分泌的。
已經可以採用志賀氏菌屬的III型機制表達異源性蛋白質(見″Functional conservation of the Salmonella and Shigella effectors of entryinto epithelial cells″，發表於Molecular Microbiology(1995) 17(4)，781-789，Hermant，Ménard，Arricau，Parsot and Popoff)。不過，如標題所示，該文獻涉及沙門氏菌屬的蛋白質，後者不同於衣原體蛋白質，因為衣原體在系統發生方面與其他生物體之間存在距離(見page 369，first columnof Subtil，et al.；Microbes and Infection 2，2000；Subtil A.，A.Blocker，andA.Dautry-Varsat.(2000).Type III secretion system in Chlamydiaidentifiedmembers and candidates.Microbes Infect.2367-369)。
此外，WO 99/58714已經披露了用於在志賀氏菌屬中篩選革蘭氏陰性細菌的III型分泌機制的抑制劑和激活劑的方法。
包括衣原體在內的一些革蘭氏陰性病原體具有用於將細菌蛋白質插入真核細胞膜或轉運通過真核細胞膜的III型分泌機制。在美國專利申請10/014,670(將其全文併入本文作為參考)中，本發明人通過將這些蛋白質的N端與報導基因(百日咳桿菌(Bordetella pertussis)的Cya蛋白)進行融合而構建嵌合體，並在弗氏志賀氏菌(Shigella flexneri)的不同菌株中表達這些嵌合體。本領域普通技術人員熟知如何使用報導基因。將編碼一種易於被檢測的蛋白質的報導基因與感興趣的DNA片段連接。本發明所使用的報導基因百日咳桿菌cya基因見於Jones等，1998 andSory and Cornelis，1994(Jones，M.A.，M.W.Wood，P.B.Mullan，P.R.Watson，T.S.Wallis，and E.E.Galyov.(1998).Secreted effector proteins ofSalmonella dublin act in concert to induce enteritis.Infection Immunity.665799-804；Sory，M.P.，and G.R.Cornelis.(1994).Translocation of a hybridYopE-adenylate cyclase from Yersinia enterocolitica into HeLa cells.Mol.Microbiol.14583-94)。此外，本發明人已經發現，一些衣原體蛋白(包括Inc家族成員和選出的與Inc蛋白具有相似疏水性特徵的蛋白質)是通過弗氏志賀氏菌的III型分泌機制分泌的。由於這些蛋白質可能參與了衣原體在細胞內存在所必需的功能，因此鑑別此類蛋白質是非常重要的。通過能夠抑制細菌生長的非免疫治療幹預，可以在這些蛋白質的基礎上開發衣原體疫苗或抗衣原體感染治療以及開發衣原體感染診斷方法。
不過，目前依然亟需進一步闡明這些被分泌的衣原體多肽及其診斷和治療用途。
在本發明中，本發明人描述了衣原體屬在感染過程中所分泌的一些新的蛋白質。絕大多數這些蛋白質來自於人類病原體沙眼衣原體和肺炎衣原體，而少數蛋白質來自於一種人類和動物病原體鸚鵡熱衣原體。在一些情況中，本發明人已經證實，如果一種蛋白質被一種菌株分泌，那麼來自其他菌株的同源蛋白質也是分泌型的(見下，類別1)。因此，認識到一種蛋白質在一種菌株中是分泌型的，將提示該蛋白質在另一種菌株中也是分泌型的，而且可以採用在此所描述的方法對此加以檢驗。因此，在此展示的結果同樣可推廣至其他衣原體菌株，在此基礎上來開發用於人類或動物的衣原體疫苗或抗衣原體感染的治療方法，以及用於開發人類或動物的衣原體感染診斷方法。

發明內容
本發明的一個目的在於提供一種來自衣原體屬菌株(例如肺炎衣原體、沙眼衣原體、貓心衣原體或鸚鵡熱衣原體)的純化的分泌型多肽。所述純化的多肽可通過如下方法篩選，該方法包含(a)提供一種重組表達載體，所述重組表達載體至少含有一種編碼感興趣的多肽的多核苷酸；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述多核苷酸表達產物的分泌；其中所述表達產物的分泌說明其相應於一種分泌型衣原體多肽。通過檢測細菌外是否存在表達產物而檢測該表達產物的分泌。
或者，純化的多肽可通過如下方法篩選，該方法包含(a)提供一種重組表達載體，所述重組表達載體至少包含融合於報導基因的編碼所述感興趣的多肽的DNA(即該重組表達載體包含一種融合構建體，其中構建體的一個成分是感興趣的多核苷酸，而構建體的第二個部分是報導基因的多核苷酸序列)；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述報導基因表達產物的分泌；其中所述表達產物的分泌說明所述融合的DNA(即(a)的融合構建體，其含有感興趣的多核苷酸和報導基因的多核苷酸序列)至少含有相應於分泌型衣原體多肽的多核苷酸。通過檢測細菌外是否存在表達產物而檢測該表達產物的分泌。
本發明的另一個目的在於提供一種純化的多核苷酸，所述的多核苷酸編碼至少一種自衣原體分泌的多肽。
本發明的另一個目的在於提供一種免疫原性組合物，所述組合物包含分泌型衣原體多肽或其免疫原性片段。
本發明的另一個目的在於提供一種針對衣原體感染的預防接種組合物，所述組合物包含分泌型衣原體多肽或其免疫原性片段。所述組合物是人類和/或獸醫學預防接種組合物。例如，所述感染是性傳播疾病、呼吸道疾病(例如肺炎或支氣管炎)，或所述感染促成動脈粥樣硬化。
本發明的另一個目的在於提供一種有效抗衣原體感染的治療組合物，所述組合物包含一種分子，該分子抑制分泌型衣原體多肽的分泌。
本發明的另一個目的在於提供一種複合體，該複合體包含分泌型衣原體多肽以及針對所述多肽的抗體。
本發明的另一個目的在於提供一種抗衣原體的抗體，其中所述抗體針對分泌型衣原體多肽或其免疫原性片段，其中所述抗體是單克隆抗體或多克隆抗體。
本發明的另一個目的在於提供用於診斷動物的衣原體感染的方法，所述動物包括人。用於診斷動物的衣原體感染的方法包含(a)提供衣原體的分泌型多肽或其免疫原性片段，任選地被標記；(b)將所述多肽或其免疫原性片段與所述動物的血清樣品相接觸；以及(c)檢測所述多肽或其免疫原性片段與血清樣品中含有的抗體之間形成的複合體；其中所述複合體是表明所述動物存在衣原體感染的指徵。
或者，用於診斷包括人在內的動物的衣原體感染的方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)將所述樣品與抗衣原體的抗體相接觸，其中所述抗體針對純化的衣原體分泌型多肽；以及(c)檢測抗原抗體的複合(antigen-antibody complexion)；其中所述複合是表明所述動物存在衣原體感染的指徵。
在本發明的目的中，所述純化的衣原體多肽可通過其在含有III型分泌途徑的革蘭氏陰性細菌菌株中的表達和分泌而鑑定。
本發明的另一個目的在於提供一種在包括人在內的動物中檢測衣原體的方法，該方法使用選自表I或表II的探針或引物對。在動物中檢測衣原體的方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)將選自表I和表II中給出的列表中的引物對加至所述組織樣品中；(c)擴增編碼相應於所選定的引物對的蛋白質的多核苷酸；以及(d)根據所述多核苷酸的存在與否來檢測衣原體的存在。
本發明的另一個目的在於提供一種在包括人在內的動物中檢測衣原體的方法，所述方法使用一種探針，所述探針在嚴格性條件下與編碼本發明的多肽的多核苷酸雜交。在動物中檢測衣原體的方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)加入雜交探針，所述探針在嚴格性條件下與編碼本發明的多肽的多核苷酸雜交；以及(c)根據所述多核苷酸的存在與否來檢測衣原體的存在。
本發明的另一個目的在於提供一種在包括人在內的動物中檢測衣原體的方法，所述方法使用一種與本發明的多肽形成複合體的抗體，抗體任選地被標記。在動物中檢測衣原體的方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)在適合複合體形成的條件下加入一種與本發明的多肽形成複合體的抗體，抗體任選地被標記；以及(c)依據所述多肽的存在與否而檢測衣原體的存在。
本發明的另一個目的在於提供一種用於表達分泌型衣原體多肽的重組質粒。
本發明的另一個目的在於提供一種重組原核細胞，例如重組革蘭氏陰性細菌菌株，該重組原核細胞以至少包含編碼分泌型衣原體多肽的多核苷酸的載體轉染。
本發明的另一個目的在於提供一種預防或治療包括人在內的動物衣原體感染的方法，其包含施用有效量的本發明的純化的分泌型衣原體多肽或其免疫原性片段。
本發明的另一個目的在於提供一種預防或治療動物衣原體感染的方法，其包含給有需要的動物施用有效量的抗體，所述抗體針對本發明的分泌型衣原體多肽或其免疫原性片段。
本發明的仍另一個目的在於提供一種篩選抑制分泌型衣原體多肽的分泌的活性分子的方法，其包含(a)將活性分子提供給衣原體培養物；(b)在適合所述活性分子對所述培養物發揮活性的條件下生長或孵育所述培養物一段時間；(c)將相應於衣原體多肽的引物對加至所述培養物中；(d)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(e)根據所述多核苷酸的存在與否來檢測分泌型衣原體多肽的存在。
本發明的進一步的目的在於提供一種檢測衣原體多肽的存在與否的方法，所述方法使用在嚴格性條件下與編碼所述多肽的多核苷酸雜交的探針，或者使用與所述多肽形成複合體的抗體，所述抗體任選地被標記。
本發明的另一個目的在於提供一種篩選抑制分泌型衣原體多肽的分泌的活性分子的方法，其包含(a)將活性分子提供給衣原體培養物；(b)在適合所述活性分子對所述培養物發揮活性的條件下生長或孵育所述培養物一段時間；以及(c)評估本發明的多肽的存在。
以上目的著重強調了本發明的某些特定方面。本發明的其他的目的、方面和實施方式見於以下的發明詳述部分。


參閱以下附圖並結合以下的詳細描述將使得本發明更加易於理解，因此可容易地全面理解本發明及其具有的其他許多優越之處。
圖1說明在志賀氏菌屬中測定分泌。志賀氏菌屬的ipaB突變菌株通過III型途徑而組成型分泌高水平的效應物。以不同的嵌合構建體轉染該菌株並在菌落上探查所述嵌合體的分泌。在早晨，在新鮮的LB平板上挑出表達不同構建體的菌落，並在37℃孵育8小時。然後以PVDF膜覆蓋菌落並在37℃生長過夜。使用抗環化酶抗體，以Western印跡在該膜上檢測嵌合體的分泌。分泌的蛋白在菌落周圍形成暈輪(下方兩排)，而非分泌蛋白僅在菌落已經生長的位置才能檢測的到(上方兩排)。
圖2志賀氏菌屬分泌全長衣原體蛋白。志賀氏菌屬的ipaB突變株通過III型途徑而組成型分泌高水平的效應物，而mxiD突變株完全缺乏III型分泌。在實驗中，以Psi1058轉化菌株，並以Western印跡檢測培養物上清液中的蛋白。在ipaB株培養物的上清液(S)部分中發現了Psi1058，但在mxiD株中，發現其僅僅與沉澱(P)部分相關。這一結果顯示，Psi1058在弗氏志賀氏菌中是通過III型機制分泌的。同樣的結果也見於以CPn0175、Psi705、Psi725、Psi774(SEQ ID NO124)和Psi1005(SEQ ID NO128)轉化的弗氏志賀氏菌。
圖3將以鸚鵡熱衣原體GPIC株感染24小時的HeLa細胞或未感染的HeLa細胞置於勻漿緩衝液(0.25M蔗糖，0.1％凝膠，0.5mM EGTA，3mM咪唑，pH 7.4)中，來回7次通過25G11/2注射器針頭使之破碎。將細胞以800g離心15分鐘，將沉澱重懸於凝膠樣品緩衝液(沉澱1含有未破碎的細胞、細菌和細胞核)，而將上清以541 000g離心45分鐘(沉澱2含有細胞膜和細菌，上清含有細胞質中的蛋白質)。將等量的沉澱和上清成分在含SDS的8％聚丙烯醯胺凝膠中進行電泳(SDS-PAGE)分析。電泳後將蛋白轉移至PVDF膜(Millipore Corporation，Bedford，MA)，將膜與抗Psi0705抗體進行雜交。根據生產商的說明，通過增強的化學發光法(Amersham Pharmacia Biotech)進行Western印跡和顯色。Psi0705出現於沉澱成分，也出現於上清成分(細胞質物質)。作為對照的MOMP(主要外膜蛋白質(major outer membrane protein))僅出現於沉澱成分中。
圖4將以鸚鵡熱衣原體GPIC株感染24小時的HeLa細胞進行固定並以0.05％皂角苷增加其通透性。以特異性兔抗體標記Psi0710(該抗體是針對純化的蛋白質產生的)，然後以Alexa488綴合的抗兔二抗(Molecular Probes)進行標記。用連接了冷卻的CCD照相機的落射螢光顯微鏡(epifluorescence microscope)檢測細胞。箭頭指出自包涵體膜伸出的纖維，其結合了Psi0710。
發明詳述除非有特別定義，在此所用的所有科技術語均採用生物化學、分子生物學、酶學、遺傳學、免疫學、普通內科學和獸醫學領域的普通技術人員所常規理解的含義。
根據在此所述的合適的方法和材料，與之相似或等價的任何方法和材料均可用於實施或驗證本發明。在此體積的所有出版物、專利申請、專利以及其他參考文獻均以前全文併入本申請作為參考。如有衝突，以本說明書(包括其中的定義)為準。此外，除非另有說明，否則，說明書中的材料、方法以及實施例均僅僅用於例證性描述本發明，而非旨在限制本發明。
術語「多肽」在此指的是以肽鍵相連接的若干胺基酸殘基的序列。這些胺基酸是本領域已知的並包括非修飾的和修飾的胺基酸。此外，多肽可具有一或多種本領域已知的修飾，例如糖基化、磷酸化等等。
術語「分泌型衣原體多肽」在此指的是在細菌外可檢測到的、具有至少20個胺基酸的衣原體蛋白質或所述蛋白質的片段。
術語「同源性」在此指的是來自於相同種或不同種的衣原體的兩種或兩種以上的蛋白質。根據該詞的含義，所述兩種或兩種以上的蛋白質與一種具有至少50個胺基酸的片段具有至少40％的相同性。優選地，同源性衣原體蛋白質與一種具有至少50個胺基酸的片段具有至少50％的相同性。更優選地，同源性衣原體蛋白質與一種具有至少50個胺基酸的片段具有至少60％的相同性、至少70％的相同性、至少80％的相同性、至少90％的相同性、至少95％的相同性、或100％的相同性。因此，同源性衣原體蛋白質包括在本發明的範圍內。
術語「細菌外(outside the bacteria)」在此指的是由細菌產生的本發明的特定多肽已經通過了細菌內膜、周質和外膜，並且其或者仍然結合於外膜，或者存在於細胞外基質，或者存在於宿主細胞內。
術語「抑制分泌型衣原體多肽分泌的活性分子」在此指的是能夠以任何方式降低所述分泌包括完全消除所述分泌的分子。例如，所述分泌的降低或完全消除可以是所述分子作用於III型分泌系統的結果或作用於細菌對所述多肽的表達的結果。
術語「III型分泌途徑」在此指的是多種分子的複雜結合(complexassociation of various molecules)，這些分子對於宿主中正常表達的多肽被分泌是必需的，例如見Hueck CJ.(1998)，Type III protein secretionsystems in bacterial pathogens of animals and plants.Microbiol Mol Biol Rev.62379-433。
術語「免疫原性片段」，當其涉及多肽時，指的是具有至少6個胺基酸且優選地具有至少10個胺基酸的多肽片段，所述多肽片段當被施用於真核生物體宿主時，其足以誘導免疫應答。
術語「異源性」，當其涉及蛋白質或多肽分泌系統時，指的是所述蛋白質或多肽在正常情況下不表達於一種宿主內。
可採用核酸擴增技術例如聚合酶鏈反應(PCR)來檢測核酸。或者，可採用直接雜交法或者採用限制性片段長度多態性分析來檢測核酸。
雜交方案是本領域已知的，例如可參見Sambrook等，MolecularCloningA Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)。
術語「衣原體感染」在此指的是由一種衣原體引起的動物的感染，特別是人類的感染，即沙眼衣原體、鸚鵡熱衣原體、肺炎衣原體和貓心衣原體。就本發明而言，衣原體感染引起多種疾病，包括沙眼、性傳播疾病、盆腔炎症性疾病、呼吸道疾病例如支氣管炎、肺炎及其後遺症例如動脈粥樣硬化。
在此，嚴格性雜交條件指的是這樣的條件，其能夠使得具有75％、80％、85％、90％、95％或98％同源性的多核苷酸之間發生雜交，這種同源性是採用常規同源性程序而確定的，其中的一個實例是威斯康星大學的UWGCG序列分析程序(Devereaux等，Nucl.Acids Res.12387-397(1984))。此類嚴格性雜交條件典型地包括在65℃在2X SSC和0.5％SDS中洗滌雜交反應混合物(Sambrook等，Molecular CloningALaboratory Manual，Cold Spring Harbor Laboratory，New York(1989))。當然，本領域人員能夠認識到，根據待雜交的多核苷酸的長度和多核苷酸的GC含量，條件是可以變化的，例如見Sambrook等，MolecularCloningA Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)。
在本發明中，「引物」或「探針」是指一種多核苷酸，特別是寡核苷酸，其通過合成方法或生物學方法而產生，並包括特定的核苷酸序列，其能夠與靶核苷酸序列所含的一部分雜交。同樣是在本發明中，用語「選自表I和表II所列的引物對』』是指相應於感興趣的蛋白質的單鏈正向引物和相應的反向引物。
可以使用任何已知的方法產生所定義的引物或探針以及本發明的所有其他寡核苷酸和多核苷酸，這些方法包括automated solid-phasechemical synthesis using cyanoethyl-phosphoramidite precursors。當然，也可使用其他熟知的方法構建合成引物/寡核苷酸。J.Sambrook，E.F.Fritsch and T.Maniatis，Molecular Cloning 11(2d ed.1989)。
用於擴增樣品核酸的引物可偶聯一種可檢測部分。該可檢測部分的一種優選的實例是螢光素，在使用雷射作為檢測系統的核酸測序系統中，螢光素是一種標準的標記物。不過，也可使用其他可檢測標記物，包括其他螢光團、放射性標記物、化學偶聯劑例如的生物素，其可以鏈黴抗生物素結合的酶進行檢測、以及表位標籤例如可採用抗體進行檢測的洋地黃毒甙。可以對引物進行修飾，由此在寡核苷酸中產生至少一個核苷酸的添加、缺失或取代。這也包括在合成寡核苷酸後引入已知的標記物例如放射性物質、酶、螢光物質，等等。
類似地，用於與編碼本發明的多肽的多核苷酸雜交的探針/寡核苷酸也可偶聯一種可檢測部分，例如便於對該多核苷酸進行檢測。
可用於本發明的合適的引物的實例見於表I。
本發明人已經在若干衣原體蛋白質的N端結構域與一種報導蛋白之間構建了嵌合體，所述報導蛋白是來自百日咳桿菌的鈣調蛋白依賴性腺苷酸環化酶(Cya)，並且已經測試了這些嵌合體是否被弗氏志賀氏菌分泌。在此研究中被分析的蛋白質可被分為5類。
(i)來自肺炎衣原體、沙眼衣原體和鸚鵡熱衣原體的相應的嵌合體，這些嵌合體具有同源性並且是分泌型的。序列分析表明這三種衣原體之間的關係相當遠，且因此它們之間的同源性蛋白的N端結構域的胺基酸相同性百分比通常是低的。因此，自同源性蛋白構建的嵌合體在它們的氨基末端區域幾乎不具有相同性，所述氨基末端區域含有推定的分泌信號。因此，鑑別到這三種同源性嵌合體(上述衣原體中各自一種)是分泌型的，這強烈支持相應的蛋白質是在衣原體感染過程中被分泌的。這種類別包含以下多肽CPn0330；CT083；Psi0330；CPn0379；CT053；Psi0379；CPn0595；CT476；Psi0595；CPn0648；CT529；Psi0648；CPn0671；CT550；Psi0671；CPn0710；CT666；Psi0710；CPn0761；CT610；Psi0761；CPn0774；CT606.1；Psi0774；CPn1002；CT845；Psi1002；CPn1005；CT848；Psi1005；CPn1022；CT863；Psi1022。
(ii)具有同源性的肺炎衣原體和沙眼衣原體的蛋白，並且所述蛋白的兩種相應的嵌合體是分泌型的。根據與以上就類別1給出的相同的原因，這些結果支持這些蛋白質同樣是在衣原體感染過程中被分泌的。這種類別包含以下多肽CPn0490；CT387；CPn0705；CT671；CPn0711；CT665；CPn0712；CT664；CPn0725；CT652.1；CPn0770；CT642；CPn0859；CT718；CPn0906；CT763。
(iii)分泌型肺炎衣原體蛋白質，所述蛋白質在沙眼衣原體或鸚鵡熱衣原體沒有同源物。它們可能是具有肺炎衣原體特異性的重要蛋白質。這種類別包含以下多肽CPn0009；CPn0012；CPn0028；CPn0049；CPn0063；CPn0066；CPn0067；CPn0130；CPn0132；CPn0146；CPn0167；CPn0174；CPn0175；CPn0181；CPn0210；CPn0211；CPn0220；CPn0223；CPn0226；CPn0243；CPn0267；CPn0277；CPn0284；CPn0357；CPn0365；CPn0585；CPn0829；CPn1027。
(iv)肺炎衣原體蛋白質，所述蛋白質在其他兩種已經測序的衣原體的至少一種中具有同源物，且這些蛋白質的相應的同源性嵌合體的分泌是尚未確定的。這種類別包含以下多肽CPn0026；CPn0104；CPn0186；CPn0206；CPn0291；CPn0292；CPn0405；CPn0443；CPn0480；CPn0489；CPn0556；CPn0673；CPn0681；CPn0720；CPn0746；CPn0853；CPn0879；CPn0939；CPn1019；CPn1020；CPn1032。
(v)肺炎衣原體蛋白質，所述蛋白質在其他兩種已經測序的衣原體中具有同源物，且這些蛋白質的相應的同源性嵌合體的分泌是陰性的或者尚未確定的。儘管這些結果支持這一類別中的大多數蛋白質是在感染過程中被衣原體分泌的，但本發明人認為，在獲得更多的數據之前(例如見下面以Psi1058得到的結果)，這些蛋白質作為候選蛋白不如其他4種類別的蛋白質更具有吸引力。這種類別包含以下多肽CPn0105；CPn0287；CPn0334；CPn0374；CPn0399；CPn0497；CPn0522；CPn0582；CPn0588；CPn0729；CPn0755；CPn0764；CPn0792；CPn0820；CPn0821；CPn1007；CPn1016；CPn1058。
本發明的多肽施用的劑量可有效地免疫動物，優選地是人，以抵抗衣原體感染，或者可有效地治療感染了衣原體的動物，優選地是人。在此，實現這一目標的多肽的有效量通常為大約2ng至2mg/kg體重/周，優選地大約2μg/kg/周。這一範圍包括其間所有的特定的數值以及其間所有的子區域。
在一個優選的實施方案中，所述衣原體多肽與選自如下一組中的一或多種肺炎衣原體蛋白或其片段具有同源性，所述的組由CPn0104(SEQ ID NO2)、CPn0206(SEQ ID NO4)、CPn0210(SEQ ID NO6)、CPn0399(SEQ ID NO8)、CPn0405(SEQ ID NO10)、CPn0443(SEQ IDNO12)、CPn0480(SEQ ID NO14)、CPn0489(SEQ ID NO16)、CPn0490(SEQ ID NO18)、CPn0497(SEQ ID NO20)、CPn0522(SEQID NO22)、CPn0556(SEQ ID NO24)、CPn0582(SEQ ID NO26)、CPn0588(SEQ ID NO28)、CPn0595(SEQ ID NO30)、CPn0671(SEQID NO32)、CPn0673(SEQ ID NO34)、CPn0681(SEQ ID NO36)、CPn0712(SEQ ID NO38)、CPn0720(SEQ ID NO40)、CPn0725(SEQID NO42)、CPn0729(SEQ ID NO44)、CPn0746(SEQ ID NO46)、CPn0755(SEQ ID NO48)、CPn0761(SEQ ID NO50)、CPn0764(SEQID NO52)、CPn0770(SEQ ID NO54)、CPn0774(SEQ ID NO56)、CPn0792(SEQ ID NO58)、CPn0853(SEQ ID NO60)、CPn0859(SEQID NO62)、CPn0879(SEQ ID NO64)、CPn0906(SEQ ID NO66)、CPn0939(SEQ ID NO68)、CPn1002(SEQ ID NO70)、CPn1005(SEQID NO72)、CPn1007(SEQ ID NO74)、CPn1019(SEQ ID NO76)、CPn1020(SEQ ID NO78)、CPn1032(SEQ ID NO80)和CPn1058(SEQID NO82)組成。
在另一個實施方案中，所述同源性衣原體多肽是選自如下一組中的沙眼衣原體蛋白或其片段，所述的組由CT387(SEQ ID NO84)、CT476(SEQ ID NO86)、CT550(SEQ ID NO88)、CT606.1(SEQ ID NO90)、CT610(SEQ ID NO92)、CT642(SEQ ID NO94)、CT652.1(SEQ ID NO96)、CT664(SEQ ID NO98)、CT718(SEQ ID NO100)、CT763(SEQID NO102)、CT845(SEQ ID NO104)和CT848(SEQ ID NO106)組成。
在另一個實施方案中，所述同源性衣原體多肽是選自如下一組中的鸚鵡熱衣原體蛋白或其片段，所述的組由Psi0330(SEQ ID NO108)、Psi0379(SEQ ID NO110)、Psi0595(SEQ ID NO112)、Psi0648(SEQ IDNO114)、Psi0671(SEQ ID NO116)、Psi0705(SEQ ID NO118)、Psi0710(SEQ ID NO120)、Psi0761(SEQ ID NO122)、Psi0774(SEQ IDNO124)、Psi1002(SEQ ID NO126)、Psi1005(SEQ ID NO128)、Psi1022(SEQ ID NO130)和Psi1058(SEQ ID NO132)組成。
在此，動物、優選人的衣原體感染包括多種疾病，包括沙眼、性傳播疾病、盆腔炎症性疾病、呼吸道疾病如支氣管炎、肺炎及其後遺症，如動脈粥樣硬化。
在本發明的範圍內，其他可預見的動物的實例包括馬(馬科動物)、牛(牛科動物)、豬(豬科動物)、綿羊(綿羊屬動物)、山羊(山羊屬動物)、鳥、狗和貓。
本發明的多肽可作為藥物組合物而施用，該藥物組合物含有該多肽化合物和藥物學可接受的載體或稀釋劑。這些活性物質可與其他活性物質相混合，所述其他活性物質應不會損害所需的作用和/或可增強所需的作用。可以採用任何途徑來施用本發明的活性物質，例如口服、胃腸外、靜脈、皮內、皮下或者局部，所述活性物質可以是液體或者固體形式。
為進行胃腸外治療性給藥，可將活性成分加入溶液或懸液中。所述溶液或懸液還可包括以下組分無菌稀釋劑如注射用水、鹽水、不揮發性油、聚乙二醇、甘油、丙二醇或其他合成溶劑；抗菌劑如苯甲醇或甲基對羥基苯甲酸酯(methyl parabens)；抗氧化劑如抗壞血酸或亞硫酸氫鈉；螯合劑如EDTA；緩衝劑如乙酸鹽、檸檬酸鹽或磷酸鹽以及滲透壓調節劑如氯化鈉或葡萄糖。胃腸外製劑可置於安瓿、一次性注射器或由玻璃或塑料製成的多劑量小瓶。
本發明的多肽的另一種施用方式是口服。口服的組合物通常包括惰性稀釋劑或可食用的載體。為進行口服治療性給藥，上述多肽可與賦形劑混合，並可採用片劑、凝膠膠囊、錠劑、膠囊、西也劑(elixirs)、懸液、糖漿、餅狀片劑(wafers)、口香糖等形式。可採用藥物組合物製備領域的任何已知的方法製備組合物，所述組合物可含有選自以下一組中的一或多種成分甜味劑、調味劑、著色劑和防腐劑。片劑含有活性成分，其中可以混合適合用於生產片劑的非毒性藥物學可接受的賦形劑。這些賦形劑可以是，例如，惰性稀釋劑如碳酸鈣、碳酸鈉、乳糖、磷酸鈣或磷酸鈉，粒化和崩解劑如玉米澱粉或褐藻酸；結合劑如澱粉、凝膠或阿拉伯樹膠(acacia)；以及潤滑劑如硬脂酸鎂、硬脂酸或滑石粉。片劑可不帶有塗層，或者可採用已知的技術進行塗層，以延緩其在胃腸道中的崩解和吸收，並由此在較長的時間內提供持續的作用。例如，可單獨使用延時物質如單硬脂酸甘油酯或二硬脂酸甘油酯或及其與蠟同時使用。用於口服的製劑還可以是硬凝膠膠囊，其中的活性成分混合了惰性固體稀釋劑，例如碳酸鈣、磷酸鈣或高嶺土，或者是軟凝膠膠囊，其中的活性成分混合了水性或者油性介質，例如花生油、液體石蠟或橄欖油。
片劑、丸劑、膠囊、錠劑等等可含有以下成分粘合劑，如微晶纖維素、黃蓍膠或凝膠；賦形劑，如澱粉或乳糖；崩解劑，如褐藻酸、Primogel、玉米澱粉等等；潤滑劑，如硬脂酸鎂或Sterotes；助流劑(glidant)，如膠狀二氧化矽；以及甜味劑如蔗糖或糖精，或者調味劑如薄荷油、甲基水楊酸，或可加入橙味調味劑。如果劑量單位形式是膠囊，除上述物質以外，其還可含有液體載體如脂肪酸。其他的劑量單位形式可含有改變劑量單位的外形的其他不同的物質，例如作為塗層。因此，片劑或者丸劑可以塗上糖、蟲膠(shellac)、或者其他腸衣塗層。糖漿中除了活性多肽以外還可含有蔗糖作為甜味劑以及一些防腐劑、染料和色素以及香料。用於製備這些不同的組合物的物質的純度應該滿足製藥學或者獸醫學的要求，並且使用的量不產生毒性。
本發明的水性懸液含有活性物質並混合有適合於製備水性懸液的賦形劑。此類賦形劑包括懸浮劑如羧甲基纖維素鈉、甲基纖維素、羥基丙基乙基纖維素(hydroxypropylethyl cellulose)、藻酸鈉、聚乙烯吡咯烷酮、黃蓍膠和阿拉伯樹膠，以及分散劑或溼潤劑如天然存在的磷脂(如卵磷脂)、烯化氧與脂肪酸的縮合產物(如聚氧乙烯硬脂酸酯)、乙撐氧與長鏈脂肪醇的縮合產物(如heptadecaethylene oxycetanol)、乙撐氧與衍生自脂肪酸和己糖醇的偏酯的縮合產物(如聚氧乙烯山梨糖醇單油酸酯)、乙撐氧與衍生自脂肪酸和己糖醇酐的偏酯的縮合產物(如聚氧乙烯脫水山梨糖醇單油酸酯)。水性懸液也可含有一或多種防腐劑如對羥基苯甲酸乙酯或對羥基苯甲酸正丙酯(ethyl or n-propyl p-hydroxybenzoate)，一或多種著色劑，一或多種調味劑和一或多種甜味劑，如蔗糖、阿斯巴甜糖、糖精或蔗糖素(sucralose)。
油性懸液可通過將活性成分懸浮於植物油中而配製，所述植物油例如為花生油、橄欖油、芝麻油或椰子油，或懸浮於礦物油如液體石蠟中而配製。油性懸液可含有增稠劑如蜂蠟、硬質石蠟或鯨蠟醇。可加入甜味劑以提供可口的口服製劑。可加入抗氧化劑例如抗壞血酸以保存這些組合物。
可用活性成分混合分散劑、懸浮劑和/或溼潤劑以及一或多種防腐劑而配製適合用於通過添加水而製備成水性懸液的本發明的分散型粉末和顆粒。合適的分散劑或溼潤劑和懸浮劑的實例如上所述。也可使用其他賦形劑，如甜味劑、調味劑和著色劑。
本發明的組合物也可以是水包油(oil-in-water)乳劑的形式。油相可以是植物油如橄欖油或花生油、礦物油如液體石蠟、或上述之混合。合適的乳化劑包括天然存在的膠質物如阿拉伯樹膠和黃蓍膠、天然存在的磷脂如大豆卵磷脂、衍生自脂肪酸和己糖醇酐的酯類或偏酯如脫水山梨糖醇單油酸酯、和這些偏酯與乙撐氧的縮合產物如聚氧乙烯脫水山梨糖醇單油酸酯。乳狀液也可含有甜味劑和調味劑。
可用甜味劑配製糖漿和西也劑，例如用甘油、山梨醇或蔗糖。此類配製品也可含有潤滑劑、防腐劑、調味劑或著色劑。
本發明的組合物的形式可以是無菌注射製劑，如無菌注射水性或油性懸液。可以按照現有技術使用以上所述的合適的分散劑或溼潤劑和懸浮劑配製這種懸液。無菌注射製劑還可以是溶於無毒性的可胃腸外用稀釋劑或溶劑中的無菌溶液或懸液，如1，3-丁二醇的溶液。可採用的可接受的載體和溶劑例如是水和林格氏液，後者是等張氯化鈉溶液。此外，無菌不揮發性油通常可用作溶劑或懸浮介質。為此，可使用任何溫和的不揮發性油，包括合成的單甘油酯或二甘油酯。此外，注射製劑中也可使用脂肪酸如油酸。可採用本領域普通技術人員熟知的常規方法進行滅菌，如通過無菌過濾法、照射法或終末滅菌法(如高壓滅菌)。
可將水性配製品(即水包油乳狀液、糖漿、西也劑和注射製劑)配製為達到最佳的穩定pH值。可採用本領域普通技術人員熟知的常規方法確定最佳pH值。也可使用合適的緩衝液來維持配製品的pH值。
本發明的多肽還可以栓劑的形式用於直腸給藥。可通過將藥物與合適的非刺激性賦形劑混合而製備這些組合物，所述非刺激性賦形劑在常溫下是固體，但在直腸溫度下是液體，由此可在直腸中融化並釋放出藥物。這些物質的非限制性實例是可可油和聚乙二醇。
產生針對特定的肽或其片段的抗體的方法是本領域普通技術人員熟知的。可通過給動物注射本發明的免疫原性肽或其免疫原性片段，並自所述動物回收能夠與所述免疫原性肽或其片段形成複合物的抗體而獲得抗體。這些方法的實例見於Antibodies，A Laboratory Manual，Harlow andLane，Cold Spring Harbor Press，1988，將該出版物併入本申請作為參考。
能夠進行單克隆或單克隆抗體人源化的技術已經在以下文獻中公開Waldmann T.(1991).Science.2521657-1662；Winter G.等(1993).Immunology Today.14(6)243-246；Carter等(1992).Proc.Natl.Acad.Sci，USA.894285-4289；Singer等(1993).Journal of Immunology.150(7)2844-2857；Kipriyanov等，Mol.Biotechnol.，2004，26(1)39-60；美國專利6,693,176；美國專利5,225,539；美國專利6,572,857；美國專利6,183,744，均以其全部內容併入本申請作為參考。
表達IncA/cya的弗氏志賀氏菌的ipaB突變株，2000年12月13日保藏於C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法國)，保藏號為I-2592。
攜帶載體pUC19cya的菌株，2000年12月13日保藏於C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法國)，保藏號為I-2593。
弗氏志賀氏菌的ipaB-突變株，命名為SF620，2000年12月13日保藏於C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法國)，保藏號為I-2594。
攜帶置於表達載體(pQE trisystem，Qiagen)中的具有C端組氨酸標籤的Psi0710的大腸桿菌菌株，2003年2月18日保藏於C.N.C.M.(25，Rue de Docteur Roux，F-75724，巴黎Cedex 15，法國)，保藏號為I-2974。
實施例在對本發明進行一般性描述之後，通過參考在此給出的一些具體實施例可獲得對本發明的進一步的理解，這些實施例僅用於說明本發明，除非另有說明，其無意於對本發明做出限制。
實施例1材料和方法菌株和試劑菌株M90T是弗氏志賀氏菌5的致病性、野生型菌株(Sansonetti等，1982)。菌株SF401和SF620(保藏於C.N.C.M.，保藏號為I-2594)是M90T的衍生物，其中mxiD和ipaB基因分別被滅活(Allaoui，A.，P.J.Sansonetti，and C.Parsot.(1993).MxiD，an outer membrane proteinnecessary for the secretion of the Shigella flexneri lpa invasins.Mol.Microbiol.759-68；Ménard，R.，P.Sansonetti and C.Parsot.(1994).Thesecretion of the Shigella flexneri Ipa invasins is activated by epithelial cellsand controlled by IpaB and IpaD.EMBO J.135293-302)。E.coli菌株TG1(Sambrook等，Molecular CloningA Laboratory Manual，Cold SpringHarbor，New York，1989)被用於構建質粒。弗氏志賀氏菌和E.coli菌株培養於Luria-Bertani(LB)培養基中。使用的氨苄青黴素的濃度為0.1mg/ml。使用了針對鈣調蛋白依賴性腺苷酸環化酶的單克隆抗體(Brézin等，(1987)Evidence for the presence of cAMP，cAMP receptor andtranscription termination factor Rho in different Gram-negative bacteria.J.Gen.Microbiol.131(11)2953-2960)。抗IpaD抗體的用法如文獻所述(Ménard，R.，P.J.Sansonetti，and C.Parsot.(1993))。用於增強的化學發光法的辣根過氧化物酶綴合的二抗來自Amersham Pharmacia Biotech(Orsay，France)。用於增強的化學發光法的鹼性磷酸酶綴合的二抗來自Pierce(Rockford，IL，USA)。
基因組數據通過對肺炎衣原體CWL029菌株進行測序而獲得的肺炎衣原體蛋白序列(CPnXXXX)可由http//chlamydia-www.berkeley.edu4231/(染色體序列Genbank登記號AE001363)得到，也可參見Kalman等，(1999)Nature Genetics.21385-389。通過對沙眼衣原體的沙眼生物型血清型D(D/UW-3/Cx)進行測序得到沙眼衣原體蛋白序列(CTXXX)，該序列可由http//chlamydia-www.berkeley.edu4231/(染色體序列Genbank登記號AE001273)得到，也可參見Stephens等，(1998)Science.282754-759。自Institute for Genomic Research的網頁http//www.tigr.org得到了由Institute for Genomic Research(TIGR，Rockville，MD，Etats-Unis)對鸚鵡熱衣原體(Chlamydophila psittaci)(GPIC菌株)進行的測序數據，該數據是不完全的，僅僅是初步的序列數據。本發明人使用了由http//tigrblast.tigr.org/ufmg/提供的對比工具將肺炎衣原體蛋白與得到的序列的6個可讀框翻譯區進行了對比。在此，與來自肺炎衣原體的被比對蛋白(CPnXXXX)最匹配的來自鸚鵡熱衣原體的蛋白被稱為PsiXXXX。
隨本說明書附上本發明的代號為CPnXXXX、CTXXX和PsiXXXX的完整DNA序列和胺基酸序列。用於構建嵌合體的相應蛋白質的胺基酸序列與大寫字母表示。
構建表達雜合蛋白質的重組質粒使用RapidPrep Micro Genomic DNA分離試劑盒(AmershamPharmacia Biotech)自純化的細菌中製備來自肺炎衣原體菌株TW183(Kuo等，(1995)Clinical Microbiology Reviews.8(4)451-461)、沙眼衣原體的沙眼生物型血清型D(D/UW-3/Cx)和鸚鵡熱衣原體(Chlamydophilapsittaci)GPIC菌株(美國典型培養物保藏中心，Virginia，U.S.A.；ATCCNo.VR-2282)的基因組DNA。以菌株TW183作為模板，通過PCR擴增實施例中所使用的肺炎衣原體DNA片段。這些片段經過測序顯示出與CWL029菌株的序列具有超過99％的相同性。使用表1所列的引物，通過PCR擴增不同衣原體基因的5′部分，包括位於推定的翻譯起始位點上遊的大約30個核苷酸和前20至99個密碼子。對於Inc/cya構建體，正向和反向引物分別含有額外的HindIII和XbaI位點，以便將PCR片段克隆入puc19cya載體的HindIII和XbaI位點之間。該載體是通過將攜帶百日咳桿菌的cya基因(Sory，M.P.，and G.R.Cornelis.(1994))的質粒pMS109的XbaI-EcoRI片段克隆至pUC19載體的XbaI和EcoRI位點之間而構建的。在重組質粒中，雜合基因的轉錄處於載體的lac啟動子的控制。在E.coli TG1中擴增重組質粒並測序以核對所有構建體的序列。核酸測序的方法是本領域人員已知的，可參見例如Sambrook等，Molecular CloningA Laboratory Manual，Cold Spring Harbor Laboratory，New York(1989)和Current Protocols in Molecular Biology，Ausebel等，eds.，John Wiley and Sons，Inc.，New York(2000)。
表1用於構建Inc/cya嵌合體的寡核苷酸。

實施例11.
增塑溶膠配方下述增塑溶膠成份與(50、51、52和53)所示穩定劑混合，除去氣泡，傾倒在一個25mil厚度的金屬平板上。凝膠作用在204℃下進行3分鐘。穩定性試驗在204℃的爐中進行，每隔2分鐘取樣，返黃值(YI)如下所示

(1)注GY-250(Ciba Specialty Chemicals，Tarrytown，New York)是一種市場上可購得的環氧樹脂，由雙酚A和epichloridrin發生反應而製成。平均分子量≤700。
由於可以在不偏離本發明的原則下做出許多變化和改進，對本發明要求保護的範圍的理解應根據所附的權利要求書。
陰性對照-預期與其他細菌的胞漿蛋白具有同源性的衣原體蛋白不會在衣原體感染過程中被分泌，且因此在本試驗中不應該表現為分泌陽性。為了驗證本試驗的特異性，本發明人在推定的胞漿衣原體蛋白和cya報導分子之間構建了9種嵌合體。這些嵌合體中無一被弗氏志賀氏菌的ipaB菌株分泌。這一結果說明，本發明人開發的這種試驗對檢測分泌型蛋白質具有特異性。
構建的9種構建體使用的是CPn0032、CPn0089、CPn0l03、CPn0115、CPn0184、CPn0202、CPn0280、CPn0320、CPn0402的氨基末端區域。
陰性結果-本發明人測試的280種構建體中的大多數不被ipaB菌株分泌。這一結果是預料之中的，這是由於在其他細菌中，例如志賀氏菌屬中，III型分泌僅能分泌數量有限的蛋白質(對於弗氏志賀氏菌，目前已經鑑定了大約25種)(Buchrieser C.，P.Glaser，C.Rusniok，H.Nedjari，H.d』Hauteville，F.Kunst，P.Sansonetti，and C.Parsot.(2000).The virulenceplasmid pWR 100 and the repertoire of proteins secreted by the type IIIsecretion apparatus of Shigella flexneri.Mol.Microbiol.38(4)760-771)。
相應的嵌合體不被ipaB菌株分泌的衣原體蛋白質的實例為CPn0010、CPn0011、CPn0034、CPn0036、CPn0042、CPn0046、CPn0050、CPn0051、CPn0055、CPn0062、CPn0070、CPn0071、CPn0084、CPn0085、CPn0087、CPn0089、CPn0093、CPn0095、CPn0099、CPn0100。
檢測由弗氏志賀氏菌通過III型分泌系統分泌的蛋白質，測定了衣原體蛋白質的各種嵌合體形式(由這些蛋白質的氨基末端結構域與Cya報導分子相融合而組成)的分泌。這些分泌型嵌合體蛋白可分為5種類別類別1—來自肺炎衣原體、沙眼衣原體和鸚鵡熱衣原體的具有同源性並被分泌的相應的嵌合體。序列分析顯示，這三種衣原體之間的關係相當遠，且因此它們之間的同源性蛋白的胺基酸相同性百分比通常是低的。因此，自同源性蛋白構建的嵌合體在它們的氨基末端區域幾乎不具有相同性，所述氨基末端區域含有推定的分泌信號。因此，鑑別到這三種同源性嵌合體(上述衣原體中各自一種)是分泌型的，這強烈支持相應的蛋白質是在衣原體感染過程中被分泌的。
屬於這種類別的肺炎衣原體(CPnXXXX)、沙眼衣原體(CTXXX)和鸚鵡熱衣原體(PsiXXXX)的蛋白質是CPn0330；CT083；Psi0330；CPn0379；CT053；Psi0379；CPn0595；CT476；Psi0595；CPn0648；CT529；Psi0648；CPn0671；CT550；Psi0671；CPn0710；CT666；Psi0710；CPn0761；CT610；Psi0761；CPn0774；CT606.1；Psi0774；CPn1002；CT845；Psi1002；CPn1005；CT848；Psi1005；CPn1022；CT863；Psi1022。
類別2—具有同源性的肺炎衣原體和沙眼衣原體的蛋白，並且所述蛋白的兩種相應的嵌合體是分泌型的。根據與以上就類別1給出的相同的原因，這些結果支持這些蛋白質是在衣原體感染過程中被分泌的。
屬於這種類別的肺炎衣原體(CPnXXXX)和沙眼衣原體(CTXXX)的蛋白質是CPn0490；CT387；CPn0705；CT671；CPn0711；CT665；CPn0712；CT664；CPn0725；CT652.1；CPn0770；CT642；CPn0859；CT718；CPn0906；CT763。
類別3—分泌型肺炎衣原體蛋白質，所述蛋白質在沙眼衣原體或鸚鵡熱衣原體沒有同源物。它們可能是具有肺炎衣原體特異性的重要蛋白質。
屬於這種類別的肺炎衣原體蛋白(CPnXXXX)是CPn0009；CPn0012；CPn0028；CPn0049；CPn0063；CPn0066；CPn0067；CPn0130；CPn0132；CPn0146；CPn0167；CPn0174；CPn0175；CPn0181；CPn0210；CPn0211；CPn0220；CPn0223；CPn0226；CPn0243；CPn0267；CPn0277；CPn0284；CPn0357；CPn0365；CPn0585；CPn0829；CPn1027。
類別4—肺炎衣原體蛋白質，所述蛋白質在其他兩種已經測序的衣原體的至少一種中具有同源物，且由於技術上或科學上的因素，對這些蛋白質的相應的同源性嵌合體的分泌尚未進行測試(例如當這些同源性蛋白質之間的相同性百分比非常高的情況下)。
屬於這種類別的肺炎衣原體(CPnXXXX)蛋白質，即那些在其他兩種已經測序的衣原體的至少一種中具有同源物的，且對其相應的同源性嵌合體的分泌尚未進行測試的肺炎衣原體(CPnXXXX)蛋白質，是CPn0026；CPn0104；CPn0186；CPn0206；CPn0291；CPn0292；CPn0405；CPn0443；CPn0480；CPn0489；CPn0556；CPn0673；CPn0681；CPn0720；CPn0746；CPn0853；CPn0879；CPn0939；CPn1019；CPn1020；CPn1032。
類別5—肺炎衣原體蛋白質，所述蛋白質在其他兩種已經測序的衣原體中具有同源物，且這些蛋白質的相應的同源性嵌合體的分泌是尚未測試的或是陰性的或者未確定的。儘管這些結果支持這一類別中的大多數蛋白質是在感染過程中被衣原體分泌的，但本發明人認為，在獲得更多的數據之前(例如見下面以Psi1058得到的結果)，這些蛋白質作為候選蛋白不如其他4種類別的蛋白質更具有吸引力。
屬於這種類別的肺炎衣原體(CPnXXXX)蛋白質，即那些在其他兩種已經測序的衣原體中具有同源物的，且對其相應的同源性嵌合體的分泌是尚未測試的或是陰性的或者未確定的肺炎衣原體(CPnXXXX)蛋白質，是CPn0105；CPn0287；CPn0334；CPn0374；CPn0399；CPn0497；CPn0522；CPn0582；CPn0588；CPn0729；CPn0755；CPn0764；CPn0792；CPn0820；CPn0821；CPn1007；CPn1016；CPn1058。
實施例2通過PCR克隆衣原體基因以表達在羧基末端具有組氨酸標籤的全長衣原體蛋白質。正向引物和反向引物分別含有額外的NcoI和KpnI位點，以便能夠將PCR片段克隆入pQE-TriSystem表達載體(Qiagen)的NcoI和KpnI位點之間。引物的序列見於表2。將重組質粒在E.coli TG1中進行擴增，並測序以便對序列進行核對。
用質粒轉化M90T的衍生物SF401和SF620菌株，SF401和SF620中的mxiD和ipaB基因已經分別被滅活(Allaoui等，1993；Ménard等，1993)。在含有100μg/ml氨苄青黴素的培養皿中分離轉化的集落。
如前所述(Allaoui等，1993)對分泌的蛋白質進行分析。簡言之，將1ml過夜培養的培養物(30℃)接種於30ml的LB中，並在37℃孵育3小時。離心收集細菌，並以0.22μm濾器過濾培養物上清。在25ml的濾出液中加入1/10(v/v)的三氯乙酸以沉澱其中的蛋白質，然後重懸於0.5ml的樣品緩衝液中進行電泳分析。在含有SDS的12％聚丙烯醯胺凝膠中進行電泳(SDS-PAGE)以分析等體積的細菌沉澱樣品和培養物上清液樣品，在所使用的樣品中，相對於沉澱樣品而言，上清液樣品被濃縮了25倍。電泳後，蛋白質倍轉移至PVDF膜(Millipore Corporation，Bedford，MA)，並使用抗組氨酸抗體(H-15，sc-803，Santa CruzBiotechnology)在膜上進行雜交。按照生產商的說明，以增強的化學發光法(Amersham Pharmacia Biotech)進行Western印跡和顯色。
表2

本發明人已經顯示，在轉化的ipaB培養物的上清液中發現了C末端具有組氨酸標籤的全長蛋白質CPn0175、Psi0705、Psi0725、Psi0774、Psi1005和Psi1058。當這些蛋白質在缺乏III型分泌的mxiD菌株中表達時，其不出現於培養物上清液中(見圖2)。這些結果證實CPn0175、Psi0705、Psi0725、Psi0774、Psi1005和Psi1058在弗氏志賀氏菌中是通過III型分泌機制分泌的。
實施例3在E.coli中表達羧基端具有組氨酸標籤的Psi0705和Psi0710蛋白質，並根據生產商(Qiagen)的說明，通過標準的二價金屬柱層析法對蛋白質進行純化。用純化的蛋白質免疫兔子，由此獲得抗Psi0705和Psi0710的特異性抗體。這些抗體用於研究Psi0705和Psi0710在衣原體感染過程中的定位。
A本發明人通過免疫螢光證實，Psi0710與感染了鸚鵡熱衣原體GPIC菌株的HeLa細胞中的包涵體膜相關(圖4)。本發明人證實，作為對照的免疫前的血清沒有對感染的細胞作出標記，並且抗衣原體主要外膜蛋白質(Major Outer Membrane Protein of Chlamydia)的抗體沒有對包涵體膜作出標記。
B本發明人通過Western印跡證實，Psi0705與感染了鸚鵡熱衣原體GPIC菌株的HeLa細胞中的細胞質成分相關(圖3)。以特異於Psi0710的抗體得到了相同的結果。本發明人證實，在感染了鸚鵡熱衣原體GPIC菌株的HeLa細胞的細胞質成分中，沒有發現作為對照的衣原體主要外膜蛋白質。
這些實驗證實，Psi0705和Psi0710是在衣原體感染過程中由衣原體分泌的。因此，這些結果充分肯定了如本申請所公開的採用異源性III型分泌來鑑定此類蛋白質的本發明的方法。
顯然，根據以上教導，可以對本發明作出各種各樣的修改和變更。因此可以理解，在所附權利要求書的範圍內，可以採用不同於在此具體公開的具體實施方式
來實施本發明。
序列表110巴斯德研究院國立科學研究中心120分泌型衣原體多肽、其編碼多核苷酸及其治療和診斷用途130BLOcp226PCT114150US 60/448,8791512003-02-24160378170PatentIn version 3.121012111203212DNA213Chlamydia pneumoniae4001atgagaatgc tccagatttc tatgcttctt ttagctttag gaactgcaat caactcacca 60gcaatctatg ctgccgattc ccaatccgta tcctttccag aacaacttcc ctcttcattt 120actggagaaa ttaagggaaa ccacgtacgg atgcgtctag cacctcatac tgatgggacc 180atcattaggg aattttctaa aggagatctt gttgctgtta tcggagaaag caaagactac 240tacgtaattt ctgcgcctcc aggaattaca ggttatgtgt tccgctcatt tgttttagat 300aatgtcgttg aaggtgaaca agtcaatgtt cgtttagaac cctcaacatc agctccagta 360cttgtgagac tctcccgagg cacacaaata cagccagctt ctcaagagcc acatgggaaa 420tggttagagg tggtcttgcc ctcacaatgc gtattctatg ttgcaaaaaa ctttgttgct 480aacaaaggac ccatcgagct gtatacgcaa cgcgagggac aaaaaaagat tgccatggac 540cttatcaatt ctgctttaaa ctttgctcat atagagcttg agaaaagcct caatgagatt 600gatctggaag caatttataa aaagatcaac cttgtacaat ccgaagagtt taaagatgtt 660ccaggaattc aagggcttat acaaaaagct ttagaagaaa tccaagatgc ctatctttct 720aaatctctag aatctcaaaa tacttcgatt gcaagctcac aatgttccac tcctaaggtt 780tcttcttctg aagttacaac ttcattactt tcacgtcata ttcgtaagca aactgcatta 840aaaacagctc ctcttaccca aggaagagaa aacctagagt attctctctt cagaatctgg 900gccagtatgc agcaaggcaa tgaccactct gaagcactaa cacaagaagc gttttatcgc 960gctgaacaga agaaaaaaca agtgcttgcg ggtgtattag aagtgtatcc tcatgtagta 1020aagaacaatc ccggggatta cctactaaaa gctcaggaaa acacgattgc ttttctttac 1080ggtacaagta tcaacttaga gcaatggtta ggaaagcgtg tcactgtgga atgtctccca 1140cgtcctaaca accattttgc ttttcctgct tattatgtag ttggaattaa agaagcttca 1200taa1203
2102211310212PRT213Chlamydia pneumoniae4002Met Arg Met Leu Gln Ile Ser Met Leu Leu Leu Ala Leu Gly Thr Ala1 5 10 15Ile Asn Ser Pro Ala Ile Tyr Ala Ala Asp Ser Gln Ser Val Ser Phe20 25 30Pro Val Leu Leu Gly Leu Gly Gln Asp Lys Phe Leu Lys Ala Thr Glu35 40 45Asp Glu Asp Val Leu Phe Glu Ser Gln Lys Ala Ile Asp Ala Trp Asn50 55 60Ala Leu Leu Thr Lys Ala Arg Asp Val Leu Gly Leu Gly Asp Ile Gly65 70 75 80Ala Ile Tyr Gln Thr Ile Glu Phe Leu Gly Ala Tyr Leu Ser Lys Val85 90 95Asn Arg Arg Ala Phe Cys Ile Ala Ser Glu Ile His Phe Leu Lys Thr100 105 110Ala Ile Arg Asp Leu Asn Ala Tyr Tyr Leu Leu Asp Phe Arg Trp Pro115 120 125Leu Cys Lys Ile Glu Glu Phe Val Asp Trp Gly Asn Asp Cys Val Glu130 135 140Ile Ala Lys Arg Lys Leu Cys Thr Phe Glu Lys Glu Thr Lys Glu Leu145 150 155 160Asn Glu Ser Leu Leu Arg Glu Glu His Ala Met Glu Lys Cys Ser Ile165 170 175Gln Asp Leu Gln Arg Lys Leu Ser Asp Ile Ile Ile Glu Leu His Asp180 185 190Val Ser Leu Phe Cys Phe Ser Lys Thr Pro Ser Gln Glu Glu Tyr Gln195 200 205Lys Asp Cys Leu Tyr Gln Ser Arg Leu Arg Tyr Leu Leu Leu Leu Tyr210 215 220Glu Tyr Thr Leu Leu Cys Lys Thr Ser Thr Asp Phe Gln Glu Gln Ala225 230 235 240
Arg Ala Lys Glu Glu Phe Ile Arg Glu Lys Phe Ser Leu Leu Glu Leu245 250 255Glu Lys Gly Ile Lys Gln Thr Lys Glu Leu Glu Phe Ala Ile Ala Lys260 265 270Ser Lys Leu Glu Arg Gly Cys Leu Val Met Arg Lys Tyr Glu Ala Ala275 280 285Ala Lys His Ser Leu Asp Ser Met Phe Glu Glu Glu Thr Val Lys Ser290 295 300Pro Arg Lys Asp Thr Glu305 3102103211756212DNA213Chlamydia pneumoniae4003ttgctaatgg atatttccca tatcctggaa gatcttgcct atgacgaagg gatccttcca 60agggaagcta tagaagcggc tattgttaaa caaatgcaaa ttacgcctta tttactgcat 120attttacacg acgctactca gcgcgtccct gagattgtaa atgatgggag ttatcaaggt 180cacctctatg ccatgtatct cctcgcacaa ttcagagaaa gtcgcgcact ccctctcatc 240attaaactct ttgcatttga agatgatact ccacacgcaa tagcaggtga tgtcctaacc 300gaagatctgc ctaggatcct agctagcgtc tgcaatgatg actcgctaat taaagagctc 360atagaaactc caaaaatcaa tccttatgtg aaggcagccg caatctctgg tcttgtaact 420cttgtaggag ccgggaaaat tcctagggat aaagttatcc gttattttgc agaacttcta 480aactatagat tagaaaaaca gccctcgttc gcttgggata acctaatcgc agggatctgt 540actctttacc ccggagagct cttctatcca ataagcaaag cctttgacgg aggacttgtt 600gatacatctt tcatcagcat ggaagatgtc gaaaatatta tccacgaaga aaccgtggaa 660tcttgtatcc ataccctctg ttcttctaca gaactcatta atgacactct agaagaaatg 720gaaaaatggt tagaagactt ccccatagaa ccgtga7562104211251212PRT213Chlamydia pneumoniae4004Leu Leu Met Asp Ile Ser His Ile Leu Glu Asp Leu Ala Tyr Asp Glu1 5 10 15
Gly Ile Leu Pro Arg Glu Ala Ile Glu Ala Ala Ile Val Lys Gln Met20 25 30Gln Ile Thr Pro Tyr Leu Leu His Ile Leu His Asp Ala Thr Gln Arg35 40 45Val Pro Glu Ile Val Asn Asp Gly Ser Tyr Gln Gly His Leu Tyr Ala50 55 60Met Tyr Leu Leu Ala Gln Phe Arg Glu Ser Arg Ala Leu Pro Leu Ile65 70 75 80Ile Lys Leu Phe Ala Phe Glu Asp Asp Thr Pro His Ala Ile Ala Gly85 90 95Asp Val Leu Thr Glu Asp Leu Pro Arg Ile Leu Ala Ser Val Cys Asn100 105 110Asp Asp Ser Leu Ile Lys Glu Leu Ile Glu Thr Pro Lys Ile Asn Pro115 120 125Tyr Val Lys Ala Ala Ala Ile Ser Gly Leu Val Thr Leu Val Gly Ala130 135 140Gly Lys Ile Pro Arg Asp Lys Val Ile Arg Tyr Phe Ala Glu Leu Leu145 150 155 160Asn Tyr Arg Leu Glu Lys Gln Pro Ser Phe Ala Trp Asp Asn Leu Ile165 170 175Ala Gly Ile Cys Thr Leu Tyr Pro Gly Glu Leu Phe Tyr Pro Ile Ser180 185 190Lys Ala Phe Asp Gly Gly Leu Val Asp Thr Ser Phe Ile Ser Met Glu195 200 205Asp Val Glu Asn Ile Ile His Glu Glu Thr Val Glu Ser Cys Ile His210 215 220Thr Leu Cys Ser Ser Thr Glu Leu Ile Asn Asp Thr Leu Glu Glu Met225 230 235 240Glu Lys Trp Leu Glu Asp phe Pro Ile Glu Pro245 2502105211948212DNA213Chlamydia pneumoniae
4005atgctagtag agttagaggc tcttaaaaga gagtttgcgc atttaaaaga ccagaagccg 60acaagtgacc aagagatcac ttcactttat caatgtttgg atcatcttga attcgtttta 120ctcgggctgg gccaggacaa atttttaaag gctacggaag atgaagatgt gctttttgag 180tctcaaaaag caatcgatgc gtggaatgct ttattgacaa aagccagaga tgttttaggt 240cttggggaca taggtgctat ctatcagact atagaattct tgggtgccta tttatcaaaa 300gtgaatcgga gggctttttg tattgcttcg gagatacatt ttctaaaaac agcaatccga 360gatttgaatg catattacct gttagatttt agatggcctc tttgcaagat agaagagttt 420gtggattggg ggaatgattg tgttgaaata gcaaagagga agctatgcac ttttgaaaaa 480gaaaccaagg agctcaatga gagccttctt agagaggagc atgcgatgga gaaatgctcg 540attcaagatc tgcaaaggaa acttagcgac attattattg aattgcatga tgtttctctt 600ttttgttttt ctaagactcc cagtcaagag gagtatcaaa aggattgttt gtatcaatca 660cgattgaggt acttattgtt gctgtatgag tatacattgt tatgtaagac atccacagat 720tttcaagagc aggctagggc taaagaggag ttcattaggg agaaattcag ccttctagag 780ctcgaaaagg gaataaaaca aactaaagag cttgagtttg caattgctaa aagtaagtta 840gaacggggct gtttagttat gaggaagtat gaagctgccg ctaaacatag tttagattct 900atgttcgaag aagaaactgt gaagtcgccg cggaaagaca cagaataa 9482106211315212PRT213Chlamydia pneumoniae4006Met Leu Val Glu Leu Glu Ala Leu Lys Arg Glu Phe Ala His Leu Lys1 5 10 15Asp Gln Lys Pro Thr Ser Asp Gln Glu Ile Thr Ser Leu Tyr Gln Cys20 25 30Leu Asp His Leu Glu Phe Val Leu Leu Gly Leu Gly Gln Asp Lys Phe35 40 45Leu Lys Ala Thr Glu Asp Glu Asp Val Leu Phe Glu Ser Gln Lys Ala50 55 60Ile Asp Ala Trp Asn Ala Leu Leu Thr Lys Ala Arg Asp Val Leu Gly65 70 75 80Leu Gly Asp Ile Gly Ala Ile Tyr Gln Thr Ile Glu Phe Leu Gly Ala85 90 95
Tyr Leu Ser Lys Val Asn Arg Arg Ala Phe Cys Ile Ala Ser Glu Ile100 105 110His Phe Leu Lys Thr Ala Ile Arg Asp Leu Asn Ala Tyr Tyr Leu Leu115 120 125Asp Phe Arg Trp Pro Leu Cys Lys Ile Glu Glu Phe Val Asp Trp Gly130 135 140Asn Asp Cys Val Glu Ile Ala Lys Arg Lys Leu Cys Thr Phe Glu Lys145 150 155 160Glu Thr Lys Glu Leu Asn Glu Ser Leu Leu Arg Glu Glu His Ala Met165 170 175Glu Lys Cys Ser Ile Gln Asp Leu Gln Arg Lys Leu Ser Asp Ile Ile180 185 190Ile Glu Leu His Asp Val Ser Leu Phe Cys Phe Ser Lys Thr Pro Ser195 200 205Gln Glu Glu Tyr Gln Lys Asp Cys Leu Tyr Gln Ser Arg Leu Arg Tyr210 215 220Leu Leu Leu Leu Tyr Glu Tyr Thr Leu Leu Cys Lys Thr Ser Thr Asp225 230 235 240Phe Gln Glu Gln Ala Arg Ala Lys Glu Glu Phe Ile Arg Glu Lys Phe245 250 255Ser Leu Leu Glu Leu Glu Lys Gly Ile Lys Gln Thr Lys Glu Leu Glu260 265 270Phe Ala Ile Ala Lys Ser Lys Leu Glu Arg Gly Cys Leu Val Met Arg275 280 285Lys Tyr Glu Ala Ala Ala Lys His Ser Leu Asp Ser Met Phe Glu Glu290 295 300Glu Thr Val Lys Ser Pro Arg Lys Asp Thr Glu305 310 3152107211648212DNA213Chlamydia pneumoniae4007atgcgaaaaa tgttggtatt attggcatct ttaggacttc tatccccaac cctatccagc 60
tgcactcact taggctcttc aggaagttat catcctaagc tatacacttc agggagcaaa 120actaaaggtg tgattgcgat gcttcctgta tttcatcgcc caggaaagag tcttgaacct 180ttaccttgga acctccaagg agaatttact gaagagatca gcaaaaggtt ttatgcttcg 240gaaaaggtct tcctgatcaa gcacaatgct tcacctcaga cagtctctca gttctatgct 300ccgattgcga atcgtctacc cgaaacaatt attgagcaat ttcttcctgc agaattcatt 360gttgctacag aactgttaga acaaaagaca gggaaagaag caggtgtcga ttctgtaaca 420gcgtctgtac gtgttcgcgt ttttgatatc cgtcatcata aaatagctct catttatcaa 480gagattatcg aatgcagcca gcctttaact accctagtca atgattatca tcgctatggc 540tggaactcaa aacattttga ttcaacgccc atgggcttaa tgcatagccg tcttttccgc 600gaagttgttg ccagagttga gggctatgtt tgtgctaact actcgtag 6482108211173212PRT213Chlamydia pneumoniae4008Met Arg Lys Met Leu Val Leu Leu Ala Ser Leu Gly Leu Leu Ser Pro1 5 10 15Thr Leu Ser Ser Cys Thr Ala Ile Thr Ser Ser Pro Gly Met Val Asn20 25 30Leu Leu Ile Gly Trp Ala Lys Thr Lys Phe Ile Gln Pro Ile Arg Glu35 40 45Ser Lys Leu Phe Gln Ser Arg Ala Cys Gln Ile Thr Leu Leu Val Leu50 55 60Gly Ile Leu Leu Val Val Ala Gly Leu Ala Cys Met Phe Ile Phe His65 70 75 80Ser Gln Leu Gly Ala Asn Ala Phe Trp Leu Ile Ile Pro Ala Ala Ile85 90 95Gly Leu Ile Lys Leu Leu Val Thr Ser Leu Cys Phe Asp Glu Ala Cys100 105 110Thr Ser Glu Lys Leu Met Val Phe Gln Lys Trp Ala Gly Val Leu Glu115 120 125Asp Gln Leu Asp Asp Gly Ile Leu Asn Asn Ser Asn Lys Ile Phe Gly130 135 140His Val Lys Thr Glu Gly Asn Thr Ser Arg Ala Thr Thr Pro Val Leu
145 150 155 160Asn Asp Gly Arg Gly Thr Pro Val Leu Ser Pro Leu Val165 1702109211777212DNA213Chlamydia pneumoniae4009ttgggtttca ctgattactt aggaggttgt ttgacgaatc ctttagggaa attcccctca 60ccacagaatc cacaggttgt tacgatagcg ccttcttcca caacaccaca agcagtctca 120tctgcagttc aaggttttct tcaaactgga ggagctgcct cctctacagc gacaactact 180accgcatccg gagcctctgc attaggactt tcacctgatc aagtgcaagc gttgcttact 240aatttattaa atgtgggaca accatcagtg ggacaaccat caacttcagc aggaacttcg 300ggagcctcct cttccagtgc aagtatgcag caacagcttt tgcaacttat cttagacaag 360acaacaggaa gtggcggatc gtccgtgagt tcagagcaat tacagcaact ccttagcttg 420gtgagccaga tgactacgtc tcaaggagga agtggtggaa ctcaggcagg acaggccgct 480tcggtactgt tgaatttgtt atcggcaaca ggatctgcag cagcaaatcc tttagggaca 540gctgcatcgt tggcacagat catttatgca gcagtaacaa gtcctggagc aaagaaaact 600agcgaatttt gttataatta ttgtggagag acctgccaag gcaactgcgg ttgtcctacc 660tgtggctgtc cagacggaca gtgcggttgt ggaggatttg gccgtttttt ctgtggtgta 720tggaaaaatt gttgcgggat aggagaggga tcccaagaac ccgcaatccc tttataa 77721010211258212PRT213Chlamydia pneumoniae40010Leu Gly Phe Thr Asp Tyr Leu Gly Gly Cys Leu Thr Asn Pro Leu Gly1 5 10 15Lys Phe Pro Ser Pro Gln Asn Pro Gln Val Val Thr Ile Ala Pro Ser20 25 30Ser Thr Thr Pro Gln Ala Val Ser Ser Ala Val Gln Gly Phe Leu Gln35 40 45Thr Gly Gly Ala Ala Ser Ser Thr Ala Thr Thr Thr Thr Ala Ser Gly50 55 60Ala Ser Ala Leu Gly Leu Ser Pro Asp Gln Val Gln Ala Leu Leu Thr65 70 75 80
Asn Leu Leu Asn Val Gly Gln Pro Ser Val Gly Gln Pro Ser Thr Ser85 90 95Ala Gly Thr Ser Gly Ala Ser Ser Ser Ser Ala Ser Met Gln Gln Gln100 105 110Leu Leu Gln Leu Ile Leu Asp Lys Thr Thr Gly Ser Gly Gly Ser Ser115 120 125Val Ser Ser Glu Gln Leu Gln Gln Leu Leu Ser Leu Val Ser Gln Met130 135 140Thr Thr Ser Gln Gly Gly Ser Gly Gly Thr Gln Ala Gly Gln Ala Ala145 150 155 160Ser Val Leu Leu Asn Leu Leu Ser Ala Thr Gly Ser Ala Ala Ala Asn165 170 175Pro Leu Gly Thr Ala Ala Ser Leu Ala Gln Ile Ile Tyr Ala Ala Val180 185 190Thr Ser Pro Gly Ala Lys Lys Thr Ser Glu Phe Cys Tyr Asn Tyr Cys195 200 205Gly Glu Thr Cys Gln Gly Asn Cys Gly Cys Pro Thr Cys Gly Cys Pro210 215 220Asp Gly Gln Cys Gly Cys Gly Gly Phe Gly Arg Phe Phe Cys Gly Val225 230 235 240Trp Lys Asn Cys Cys Gly Ile Gly Glu Gly Ser Gln Glu Pro Ala Ile245 250 255Pro Leu210112111254212DNA213Chlamydia pneumoniae40011atgagccaac cccctataaa ccctttaggt caacctcaag ttcctgcagc agcatcccca 60tcagggcagc caagcgtggt aaaacgttta aaaacgtcat ccacagggtt attcaaaaga 120tttattacta ttcctgataa atatcctaaa atgcgctatg tctatgacac aggcattatt 180gcccttgcgg caattgcgat cctttcgatt ctcctgactg cttcaggaaa cagccttatg 240ctttatgctc tcgctccggc acttgccctg ggagctttgg gagttactct acttatttct 300
gatattctgg acagtccgaa ggccaagaaa atcggtgagg caatcactgc tatcgtcgtt 360cctatcattg tattagcgat tgctgcgggt cttattgcag gggctttcgt tgcctctagt 420gggacgatgt tagtctttgc caaccctatg tttgtcatgg gattgattac ggtggggcta 480tacttcatgt ccttgaataa gctcacctta gattatttcc gtagggaaca cctcttgagg 540atggaaaaga aaacccaaga gaccgcggag cctattctag tgactccatc cgccgacgat 600gcaaaaaaaa tcgcagtgga aaagaaaaaa gatctttctg catctgcccg catggaggaa 660cacgaagctt cacaacgcca agatgcccgt catcgtagga tcggtcggga ggctcaagga 720tctttcttct attcgtcacg aaatcctgag catagacgct ccttcggcag cctctcacgt 780tttaaaacaa aaccctcaga tgcggcttct acacgacccg catctataag tcctccattt 840aaggacgatt ttcagcctta tcacttcaaa gatttaagaa gcagttcatt cggtagtgga 900gcgagcagtg cgtttacacc cataatgcct gcaagttccc gctctcctaa tttctccacg 960gggacggttc tacaccctga gccggtctac cctaagggag gaaaagaacc ctcaattcct 1020cgagtttctt catcttcccg ccgttcccct cgtgatcgcc aagataaaca gcagcaacag 1080caaaatcaag atgaagaaca gaaacagcaa tctaagaaga aaagcgggaa atcgaatcaa 1140tctcttaaaa ctccgcctcc agacggaaaa agcacggcta acctcagccc ctccaatcca 1200ttctctgacg gttatgacga aagagaaaaa cggaaacaca gaaagaacaa ataa125421012211417212PRT213Chlamydia pneumoniae40012Met Ser Gln Pro Pro Ile Asn Pro Leu Gly Gln Pro Gln Val Pro Ala1 5 10 15Ala Ala Ser Pro Ser Gly Gln Pro Ser Val Val Lys Arg Leu Lys Thr20 25 30Ser Ser Thr Gly Leu Phe Lys Arg Phe Ile Thr Ile Pro Asp Lys Tyr35 40 45Pro Lys Met Arg Tyr Val Tyr Asp Thr Gly Ile Ile Ala Leu Ala Ala50 55 60Ile Ala Ile Leu Ser Ile Leu Leu Thr Ala Ser Gly Asn Ser Leu Met65 70 75 80Leu Tyr Ala Leu Ala Pro Ala Leu Ala Leu Gly Ala Leu Gly Val Thr85 90 95
Leu Leu Ile Ser Asp Ile Leu Asp Ser Pro Lys Ala Lys Lys Ile Gly100 105 110Glu Ala Ile Thr Ala Ile Val Val Pro Ile Ile Val Leu Ala Ile Ala115 120 125Ala Gly Leu Ile Ala Gly Ala Phe Val Ala Ser Ser Gly Thr Met Leu130 135 140Val Phe Ala Asn Pro Met Phe Val Met Gly Leu Ile Thr Val Gly Leu145 150 155 160Tyr Phe Met Ser Leu Asn Lys Leu Thr Leu Asp Tyr Phe Arg Arg Glu165 170 175His Leu Leu Arg Met Glu Lys Lys Thr Gln Glu Thr Ala Glu Pro Ile180 185 190Leu Val Thr Pro Ser Ala Asp Asp Ala Lys Lys Ile Ala Val Glu Lys195 200 205Lys Lys Asp Leu Ser Ala Ser Ala Arg Met Glu Glu His Glu Ala Ser210 215 220Gln Arg Gln Asp Ala Arg His Arg Arg Ile Gly Arg Glu Ala Gln Gly225 230 235 240Ser Phe Phe Tyr Ser Ser Arg Asn Pro Glu His Arg Arg Ser Phe Gly245 250 255Ser Leu Ser Arg Phe Lys Thr Lys Pro Ser Asp Ala Ala Ser Thr Arg260 265 270Pro Ala Ser Ile Ser Pro Pro Phe Lys Asp Asp Phe Gln Pro Tyr His275 280 285Phe Lys Asp Leu Arg Ser Ser Ser Phe Gly Ser Gly Ala Ser Ser Ala290 295 300Phe Thr Pro Ile Met Pro Ala Ser Ser Arg Ser Pro Asn Phe Ser Thr305 310 315 320Gly Thr Val Leu His Pro Glu Pro Val Tyr Pro Lys Gly Gly Lys Glu325 330 335Pro Ser Ile Pro Arg Val Ser Ser Ser Ser Arg Arg Ser Pro Arg Asp340 345 350Arg Gln Asp Lys Gln Gln Gln Gln Gln Asn Gln Asp Glu Glu Gln Lys
355 360 365Gln Gln Ser Lys Lys Lys Ser Gly Lys Ser Asn Gln Ser Leu Lys Thr370 375 380Pro Pro Pro Asp Gly Lys Ser Thr Ala Asn Leu Ser Pro Ser Asn Pro385 390 395 400Phe Ser Asp Gly Tyr Asp Glu Arg Glu Lys Arg Lys His Arg Lys Asn405 410 415Lys21013211657212DNA213Chlamydia pneumoniae40013atgttaggtt ctttgccatg ttatcctggt gctggcaata ttgaagaata caaaaatagg 60tatttctatt gtcagttatg tgctgaggtc gttagtccct atgttgttcc tgttattgta 120gttgatgtgc aaggggctcc tcctacaggt atcttgcagg tcttgcgttg taagcaacat 180aaatttcaag gcctacccgt acatggcccc attacttctt tatgggcttt ggagcccgtg 240ggtaagggag ctccgcagct ggagtctgca atgtacgagc tctgttctca agtaaggaat 300tttgacatct gctctattgt gagttgggtc tttggtgggt tgtgtatttt tgcaggtctg 360attgtcgggg taatggttga agcccctttg attgcgggat taagtgcttg ggtgattccc 420tgtatcattg gaggggttgg tgccatttta tgcttgtttg cgatcttgat ggcgtacttg 480ggaagaggga gagtccgtga gtggctcaat ctttcacacg aatatataac gcaatgtcat 540tgtcgtcaga tacaggcaca ttctcaaaac tattctgtga tcacagagta tcctgcaacc 600tgtgcattat ctcaaccgat tacaaagtta cctaatggat cacgcagaga taactaa 65721014211218212PRT213Chlamydia pneumoniae40014Met Leu Gly Ser Leu Pro Cys Tyr Pro Gly Ala Gly Asn Ile Glu Glu1 5 10 15Tyr Lys Asn Arg Tyr Phe Tyr Cys Gln Leu Cys Ala Glu Val Val Ser20 25 30Pro Tyr Val Val Pro Val Ile Val Val Asp Val Gln Gly Ala Pro Pro35 40 45
Thr Gly Ile Leu Gln Val Leu Arg Cys Lys Gln His Lys Phe Gln Gly50 55 60Leu Pro Val His Gly Pro Ile Thr Ser Leu Trp Ala Leu Glu Pro Val65 70 75 80Gly Lys Gly Ala Pro Gln Leu Glu Ser Ala Met Tyr Glu Leu Cys Ser85 90 95Gln Val Arg Asn Phe Asp Ile Cys Ser Ile Val Ser Trp Val Phe Gly100 105 110Gly Leu Cys Ile Phe Ala Gly Leu Ile Val Gly Val Met Val Glu Ala115 120 125Pro Leu Ile Ala Gly Leu Ser Ala Trp Val Ile Pro Cys Ile Ile Gly130 135 140Gly Val Gly Ala Ile Leu Cys Leu Phe Ala Ile Leu Met Ala Tyr Leu145 150 155 160Gly Arg Gly Arg Val Arg Glu Trp Leu Asn Leu Ser His Glu Tyr Ile165 170 175Thr Gln Cys His Cys Arg Gln Ile Gln Ala His Ser Gln Asn Tyr Ser180 185 190Val Ile Thr Glu Tyr Pro Ala Thr Cys Ala Leu Ser Gln Pro Ile Thr195 200 205Lys Leu Pro Asn Gly Ser Arg Arg Asp Asn210 21521015211873212DNA213Chlamydia pneumoniae40015atgcagattc caagaagcat tggtactcac gatggttctt tccatgcgga tgaggtcaca 60gcgtgtgctc tccttattat tttcgatctt gtggatgaaa ataaaattat acgctctcga 120gatcctgtcg tattatcgaa atgtgaatat gtttgtgatg tcggtggtgt ttattctata 180gaaaacaagc gttttgatca tcatcaagtc tcttatgatg gatcttggag tagtgcaggt 240atgattctgc attatcttaa agagtttggt tatatggatt gtgaagaata tcatttcctt 300aacaacactt tggtacatgg tgtggatgaa caagataatg gcagattctt ctctaaggag 360ggattttgtt cgttttctga tattattaaa atttataatc ctcgcgagga agaagaaact 420
aattcggatg cggatttttc ttgtgctttg cattttacca tcgacttttt gtgtcggcta 480aggaagaagt ttcagtatga tcgagtttgt agggggattg tcagagaagc catggaaacc 540gaggatatgt gtttatattt tgatcgtcct ttagcatggc aagaaaattt ctttttttta 600gggggagaga agcaccctgc agcttttgtt tgttttcctt cctgcgatca atggatttta 660cgagggattc ctccgaattt agatcgccgt atggacgttc gtgttccttt ccctgagaat 720tgggcaggtt tgttaggtaa agagttgtcc aaagtatcag ggattcctgg ggctgtgttc 780tgccataaag gtcttttcct ttctgtatgg acaaatagag aaagttgcca acgtgctttg 840cggttaacgt tacaagatcg agggatcata tga 87321016211290212PRT213Chlamydia pneumoniae40016Met Gln Ile Pro Arg Ser Ile Gly Thr His Asp Gly Ser Phe His Ala1 5 10 15Asp Glu Val Thr Ala Cys Ala Leu Leu Ile Ile Phe Asp Leu Val Asp20 25 30Glu Asn Lys Ile Ile Arg Ser Arg Asp Pro Val Val Leu Ser Lys Cys35 40 45Glu Tyr Val Cys Asp Val Gly Gly Val Tyr Ser Ile Glu Asn Lys Arg50 55 60Phe Asp His His Gln Val Ser Tyr Asp Gly Ser Trp Ser Ser Ala Gly65 70 75 80Met Ile Leu His Tyr Leu Lys Glu Phe Gly Tyr Met Asp Cys Glu Glu85 90 95Tyr His Phe Leu Asn Asn Thr Leu Val His Gly Val Asp Glu Gln Asp100 105 110Asn Gly Arg Phe Phe Ser Lys Glu Gly Phe Cys Ser Phe Ser Asp Ile115 120 125Ile Lys Ile Tyr Asn Pro Arg Glu Glu Glu Glu Thr Asn Ser Asp Ala130 135 140Asp Phe Ser Cys Ala Leu His Phe Thr Ile Asp Phe Leu Cys Arg Leu145 150 155 160
Arg Lys Lys Phe Gln Tyr Asp Arg Val Cys Arg Gly Ile Val Arg Glu165 170 175Ala Met Glu Thr Glu Asp Met Cys Leu Tyr Phe Asp Arg Pro Leu Ala180 185 190Trp Gln Glu Asn Phe Phe Phe Leu Gly Gly Glu Lys His Pro Ala Ala195 200 205Phe Val Cys Phe Pro Ser Cys Asp Gln Trp Ile Leu Arg Gly Ile Pro210 215 220Pro Asn Leu Asp Arg Arg Met Asp Val Arg Val Pro Phe Pro Glu Asn225 230 235 240Trp Ala Gly Leu Leu Gly Lys Glu Leu Ser Lys Val Ser Gly Ile Pro245 250 255Gly Ala Val Phe Cys His Lys Gly Leu Phe Leu Ser Val Trp Thr Asn260 265 270Arg Glu Ser Cys Gln Arg Ala Leu Arg Leu Thr Leu Gln Asp Arg Gly275 280 285Ile Ile290210172112058212DNA213Chlamydia pneumoniae40017atgtataacc tactccacgc gcatcatgat gcagcctccc cagacggacg actcgtttcc 60catttgaaaa aactctcgcc ccacatttac gaaggagagg tcctcattga gaatattcct 120gcgtactttc ttggatttca tctgcctcaa cagtgtatac aagtaaattt aaaaagttcc 180ttagcccaac taggtgtcga agccgtttta aaccacttgg agctaaataa agcccgaaaa 240gaagctcgtc tacacgttct cttcatgagc caagatccta tagccactgc tatgttggag 300ctcctagagc ctggaagttt tgtctgcaag ctctttgctg ctgatgatcg ccgactcgta 360cgttcgcctt gttatctcaa caggatgttt acgcacacag accgtacagg atctccgctc 420ctacgctttg ggaaaaaact tgagcacttc atcactctag agatcattaa tgatcggctt 480gttgtcttcc ttccgatcct tccaggaaca atctgttacg aagagacaat ttatgggttc 540cttcccttaa tgagcaaatc actcacgcgt ccccatttaa aaatacgtaa gtttcttcct 600ttgtatcaaa tggtaacaga tcgtcctccc gttcccgaag atcataaaat tcttctcata 660aagacagagc ctctgcacat ccgaaccgta tttgcaagag tcgttcagga cttactcccc 720
caagggcttc gtcacaccgc agcggatatt ctcgaaccta ccacacaaga atctggagat 780atttatgaat tctacggcag cacttcagaa cctattgaga gaataccttt agaatttttt 840actcttgagc cttacaaaga gcattcgttt ttcttctata gagatatgct ccaggaaacc 900ttagaatctc ctcaagaggt atttcgtgtt tttgaatcca taccggaagg cgaagatcaa 960gctgcgatgt ttatctccaa aggtagtgag ctgcttgagc tctcccaaga ctcttggatc 1020atcaaacctc gaatctcccc atcagatgaa agacatgcta gggaaattca aaagcacatt 1080gaagaccaac cttgtttccc ttttttaaaa gccatggaaa cagatcatat cacaagccaa 1140ggagttttat tttcccgcta cttcccttca gcatcgctga agggcatgtt cctctctaac 1200tactctcgct attacctgca acatatctat tttcagattc cctctcccac ttctggagag 1260tttttctcga atcgagatcg ctctttcctt ctcgatctat attttgcagg aatttctgta 1320ttttgggcag acttagaatc gaaacgactc ttacaataca tcaaacgcag aaataaagat 1380gtgggcatgt ttgtccctaa acatcaagct gaacagtttg ctcaatccta ctttatagga 1440attcatggtt cctgcctaat cgctggggat tatgatgagt ttctccgtga gctcctgaca 1500ggaatgcata ctctttctca gcaattcacg atcccagaat ttccaccaca gacaccgtta 1560gcaatcctta caggaggggg ttctggagct atggaactcg cgaatcgtgt agctacagaa 1620ctctccatac tctcttgtgg gaatctaatt agcttggata ccacgaatgc ctatgtagaa 1680gctaaaatga gctatgctat tcctgatctt ttagaacgtc aggccgactt ccatgtcgac 1740cttgctgtat ttgttatcgg aggcatggga accgatttcg aactccttct ggagcttatt 1800agtctcaaaa cagggaaaaa agctcttgtt cccgtcttcc taatcggacc tgtagactat 1860tggaaatcca agatcacagc tttgtataat tccaatcatg ctgtaggaac cattcgaggt 1920tctgaatggg tacacaactg cctattctgc ctatcctcag caaaggcagg cattgcaatc 1980ttccgcagat atctcaatca tacgctgccc ataggacctg aacaccctgt ccctgaagat 2040ggttttgtta tcgtttag205821018211685212PRT213Chlamydia pneumoniae40018Met Tyr Asn Leu Leu His Ala His His Asp Ala Ala Ser Pro Asp Gly1 5 10 15Arg Leu Val Ser His Leu Lys Lys Leu Ser Pro His Ile Tyr Glu Gly20 25 30Glu Val Leu Ile Glu Asn Ile Pro Ala Tyr Phe Leu Gly Phe His Leu35 40 45
Pro Gln Gln Cys Ile Gln Val Asn Leu Lys Ser Ser Leu Ala Gln Leu50 55 60Gly Val Glu Ala Val Leu Asn His Leu Glu Leu Asn Lys Ala Arg Lys65 70 75 80Glu Ala Arg Leu His Val Leu Phe Met Ser Gln Asp Pro Ile Ala Thr85 90 95Ala Met Leu Glu Leu Leu Glu Pro Gly Ser Phe Val Cys Lys Leu Phe100 105 110Ala Ala Asp Asp Arg Arg Leu Val Arg Ser Pro Cys Tyr Leu Asn Arg115 120 125Met Phe Thr His Thr Asp Arg Thr Gly Ser Pro Leu Leu Arg Phe Gly130 135 140Lys Lys Leu Glu His Phe Ile Thr Leu Glu Ile Ile Asn Asp Arg Leu145 150 155 160Val Val Phe Leu Pro Ile Leu Pro Gly Thr Ile Cys Tyr Glu Glu Thr165 170 175Ile Tyr Gly Phe Leu Pro Leu Met Ser Lys Ser Leu Thr Arg Pro His180 185 190Leu Lys Ile Arg Lys Phe Leu Pro Leu Tyr Gln Met Val Thr Asp Arg195 200 205Pro Pro Val Pro Glu Asp His Lys Ile Leu Leu Ile Lys Thr Glu Pro210 215 220Leu His Ile Arg Thr Val Phe Ala Arg Val Val Gln Asp Leu Leu Pro225 230 235 240Gln Gly Leu Arg His Thr Ala Ala Asp Ile Leu Glu Pro Thr Thr Gln245 250 255Glu Ser Gly Asp Ile Tyr Glu Phe Tyr Gly Ser Thr Ser Glu Pro Ile260 265 270Glu Arg Ile Pro Leu Glu Phe Phe Thr Leu Glu Pro Tyr Lys Glu His275 280 285Ser Phe Phe Phe Tyr Arg Asp Met Leu Gln Glu Thr Leu Glu Ser Pro290 295 300
Gln Glu Val Phe Arg Val Phe Glu Ser Ile Pro Glu Gly Glu Asp Gln305 310 315 320Ala Ala Met Phe Ile Ser Lys Gly Ser Glu Leu Leu Glu Leu Ser Gln325 330 335Asp Ser Trp Ile Ile Lys Pro Arg Ile Ser Pro Ser Asp Glu Arg His340 345 350Ala Arg Glu Ile Gln Lys His Ile Glu Asp Gln Pro Cys Phe Pro Phe355 360 365Leu Lys Ala Met Glu Thr Asp His Ile Thr Ser Gln Gly Val Leu Phe370 375 380Ser Arg Tyr Phe Pro Ser Ala Ser Leu Lys Gly Met Phe Leu Ser Asn385 390 395 400Tyr Ser Arg Tyr Tyr Leu Gln His Ile Tyr Phe Gln Ile Pro Ser Pro405 410 415Thr Ser Gly Glu Phe Phe Ser Asn Arg Asp Arg Ser Phe Leu Leu Asp420 425 430Leu Tyr Phe Ala Gly Ile Ser Val Phe Trp Ala Asp Leu Glu Ser Lys435 440 445Arg Leu Leu Gln Tyr Ile Lys Arg Arg Asn Lys Asp Val Gly Met Phe450 455 460Val Pro Lys His Gln Ala Glu Gln Phe Ala Gln Ser Tyr Phe Ile Gly465 470 475 480Ile His Gly Ser Cys Leu Ile Ala Gly Asp Tyr Asp Glu Phe Leu Arg485 490 495Glu Leu Leu Thr Gly Met His Thr Leu Ser Gln Gln Phe Thr Ile Pro500 505 510Glu Phe Pro Pro Gln Thr Pro Leu Ala Ile Leu Thr Gly Gly Gly Ser515 520 525Gly Ala Met Glu Leu Ala Asn Arg Val Ala Thr Glu Leu Ser Ile Leu530 535 540Ser Cys Gly Asn Leu Ile Ser Leu Asp Thr Thr Asn Ala Tyr Val Glu545 550 555 560
Ala Lys Met Ser Tyr Ala Ile Pro Asp Leu Leu Glu Arg Gln Ala Asp565 570 575Phe His Val Asp Leu Ala Val Phe Val Ile Gly Gly Met Gly Thr Asp580 585 590Phe Glu Leu Leu Leu Glu Leu Ile Ser Leu Lys Thr Gly Lys Lys Ala595 600 605Leu Val Pro Val Phe Leu Ile Gly Pro Val Asp Tyr Trp Lys Ser Lys610 615 620Ile Thr Ala Leu Tyr Asn Ser Asn His Ala Val Gly Thr Ile Arg Gly625 630 635 640Ser Glu Trp Val His Asn Cys Leu Phe Cys Leu Ser Ser Ala Lys Ala645 650 655Gly Ile Ala Ile Phe Arg Arg Tyr Leu Asn His Thr Leu Pro Ile Gly660 665 670Pro Glu His Pro Val Pro Glu Asp Gly Phe Val Ile Val675 680 68521019211273212DNA213Chlamydia pneumoniae40019ttggatgatt catggatctt agaggttaaa gtcactccaa aagccaaaga gaacaaaatt 60gtaggctttg atggacaagc tttgaaggtc cgtgttaccg aacccccaga aaagggtaag 120gccaatgatg ctgtaatttc tttattagca aaagctttat ccttaccgaa gcgtgatgtc 180actttaattg caggagaaac ttctcgaaag aaaaagtttc ttcttcctaa cagagttcaa 240gacattattt tttctttgca tatagacgta tag 2732102021190212PRT213Chlamydia pneumoniae40020Leu Asp Asp Ser Trp Ile Leu Glu Val Lys Val Thr Pro Lys Ala Lys1 5 10 15Glu Asn Lys Ile Val Gly Phe Asp Gly Gln Ala Leu Lys Val Arg Val20 25 30Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala Val Ile Ser Leu
35 40 45Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val Thr Leu Ile Ala50 55 60Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro Asn Arg Val Gln65 70 75 80Asp Ile Ile Phe Ser Leu His Ile Asp Val85 9021021211672212DNA213Chlamydia pneumoniae40021atgaccctct acttaggatt gaatcaaaaa accgctcgta aataccaagc tcattatttg 60cctattctaa ctctcttccc ctatgcaaaa agcactccac aaaataagcg tgctcttcaa 120ttccttccac aagcaaccca tgtgattctc acaagtccct catccactca cctattcctt 180tccagaatga cttctcttct ttctaaggcc actctaaaaa caaagaccta cctctgtata 240ggagagtcca ccaaagaaag acttctctct ttccttggac aagtgaagta cgtagtagca 300actcaagaaa tcgctgaagg catcttccca ttgctacagg cactgccctc ttcagcccgc 360attctctacc cccactcctc cctcgcaaga cctgtgatca gagaatttct ttacaatcga 420tttacttttt tctcttaccc tcactacaca gtgaagccgc gaaaacttaa aaaaaatatt 480ttatctaaat acaaaaaaat tatcttcaca agcccttcaa ctgtaagagc tttcgccaaa 540atctttccgc gatttcctga aaaaacctac tggtgccaag gaaggatgac cttgcaggag 600tttcaaaagt tctcctctca aaagcaggta tctttgttag aaacgcttgg gaagtccagg 660acatctccgt ga 6722102221194212PRT213Chlamydia pneumoniae40022Met Thr Leu Tyr Leu Gly Leu Asn Gln Lys Thr Ala Arg Lys Tyr Gln1 5 10 15Ala His Tyr Leu Pro Ile Leu Thr Leu Phe Pro Tyr Ala Lys Ser Leu20 25 30Lys Val Arg Val Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala35 40 45
Val Ile Ser Leu Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val50 55 60Thr Leu Ile Ala Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro65 70 75 80Asn Arg Val Gln Asp Ile Ile Phe Ser Leu His Ile Asp Val85 9021023211570212DNA213Chlamydia pneumoniae40023atgtcatcaa atctacatcc cgtaggagga acaggaacag gagcagctgc tcctgagtct 60gtgctaaaca tagtagagga aatagcagca tcggggagtg tcaccgctgg tctacaagca 120attacgtcca gtccaggaat ggtgaatcta ctcataggat gggcaaagac aaaatttatt 180caacctatac gtgaatcaaa gctctttcaa tccagagctt gccaaattac cctgctcgtt 240ttaggaattc ttttggttgt tgctggatta gcatgtatgt ttatcttcca tagccagtta 300ggggcaaatg cattttggtt gattattcct gctgccatag gattgattaa gttactagtt 360acatcattat gttttgatga agcttgtaca tctgaaaaac tcatggtttt ccaaaaatgg 420gcaggtgttt tagaagatca gctcgatgat gggatcctta ataactcaaa taagattttt 480ggccatgtga aaacagaagg aaatacctct agggctacta ccccagtact taatgatggc 540cgcggaactc ctgtactttc acctttagta 57021024211190212PRT213Chlamydia pneumoniae40024Met Ser Ser Asn Leu His Pro Val Gly Gly Thr Gly Thr Gly Ala Ala1 5 10 15Ala Pro Glu Ser Val Leu Asn Ile Val Glu Glu Ile Ala Ala Ser Gly20 25 30Ser Val Thr Ala Gly Leu Gln Ala Ile Thr Ser Ser Pro Gly Met Val35 40 45Asn Leu Leu Ile Gly Trp Ala Lys Thr Lys Phe Ile Gln Pro Ile Arg50 55 60Glu Ser Lys Leu Phe Gln Ser Arg Ala cys Gln Ile Thr Leu Leu Val65 70 75 80
Leu Gly Ile Leu Leu Val Val Ala Gly Leu Ala Cys Met Phe Ile Phe85 90 95His Ser Gln Leu Gly Ala Asn Ala Phe Trp Leu Ile Ile Pro Ala Ala100 105 110Ile Gly Leu Ile Lys Leu Leu Val Thr Ser Leu Cys Phe Asp Glu Ala115 120 125Cys Thr Ser Glu Lys Leu Met Val Phe Gln Lys Trp Ala Gly Val Leu130 135 140Glu Asp Gln Leu Asp Asp Gly Ile Leu Asn Asn Ser Asn Lys Ile Phe145 150 155 160Gly His Val Lys Thr Glu Gly Asn Thr Ser Arg Ala Thr Thr Pro Val165 170 175Leu Asn Asp Gly Arg Gly Thr Pro Val Leu Ser Pro Leu Val180 185 19021025211828212DNA213Chlamydia pneumoniae40025atgcacgata aaaacaaggt tctgtatcta caagcaaacc atctaaatca aaaaagaaaa 60cgtcataatc ctctaaatac ataccactcc tcaaacacaa ctgaaactcg tcgcttacca 120acatactata aatccaacat tgtcttaaaa atgattttac ggatctccac cgtaagcctt 180cttacaagtt gctccttctc gaaaaattct cgtacctgtt tcgtcactcc agaacgcatt 240acctcacaaa aagactgccc cgtccttctc catccaaaaa gcactacgat ttctccccct 300ctctatgact ggatctcccc aaatagagag gtaatcaccg cctattcttt ctactgccga 360ggtcaaggaa actctatcat aactcccgaa ggggttctct atgattgtga tggactccat 420cacagcataa ctaaagaaga gttccgttat atccatccta gattgattga ggtagtacga 480ctcttgcaac aagatcaccc taaagtctct attattgaag ccttttgttg tcctaaacac 540tttcattttt tagaagcctc aggaatctca ctctctcaac tccatctcca aggtactgca 600gctaccttcg ctctagatcc tcccctcccc atggagaaac tcttggcaac tataaagaaa 660ctgtataaaa aaaactccga tccttctctc tctaatttta tcgttacaga agctacactg 720accaatccag aactgcgact cacgcaacaa gatctcggct cgcatacaga aattactgta 780gaaattctcg ataatctaca aaacaaagag gctctttcct ccgcataa 82821026
211142212PRT213Chlamydia pneumoniae40026Met His Asp Lys Asn Lys Val Leu Tyr Leu Gln Ala Asn His Leu Asn1 5 10 15Gln Lys Arg Lys Arg His Asn Pro Leu Asn Thr Tyr His Ser Ser Asn20 25 30Thr Thr Glu Thr Arg Arg Leu Pro Thr Tyr Tyr Lys Ser Asn Ile Val35 40 45Leu Lys Met Ile Leu Arg Ile Ser Thr Val Ser Leu Leu Thr Ser Cys50 55 60Ser Phe Ser Lys Asn Ser Arg Thr Cys Phe Val Thr Pro Glu Arg Leu65 70 75 80Lys Val Arg Val Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala85 90 95Val Ile Ser Leu Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val100 105 110Thr Leu Ile Ala Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro115 120 125Asn Arg Val Gln Asp Ile Ile Phe Ser Leu His Ile Asp Val130 135 14021027211546212DNA213Chlamydia pneumoniae40027atgtccttat tgaaccttcc ctcaagccag gattctgcat ctgaggactc cacatcgcaa 60tctcaaatct tcgatcccat tagaaatcgg gagttagttt ctactcccga agaaaaagtc 120cgccaaaggt tgctctcctt cctaatgcat aagctgaact accctaagaa actcatcatc 180atagaaaaag aactcaaaac tctttttcct ctgcttatgc gtaaaggaac cctaatccca 240aaacgccgcc cagatattct catcatcact ccccccacat acacagacgc acagggaaac 300actcacaacc taggcgaccc aaaacccctg ctacttatcg aatgtaaggc cttagccgta 360aaccaaaatg cactcaaaca actccttagc tataactact ctatcggagc cacctgcatt 420gctatggcag ggaaacactc tcaagtgtca gctctcttca atccaaaaac acaaactctt 480gatttttatc ctggcctccc agagtattcc caactcctaa actactttat ttctttaaac 540
ttatag 54621028211106212PRT213Chlamydia pneumoniae40028Met Ser Leu Leu Asn Leu Pro Ser Ser Gln Asp Ser Ala Ser Glu Asp1 5 10 15Ser Thr Ser Gln Ser Gln Ile Phe Asp Pro Ile Arg Asn Arg Glu Leu20 25 30Val Ser Thr Pro Glu Glu Lys Val Arg Gln Arg Leu Lys Val Arg Val35 40 45Thr Glu Pro Pro Glu Lys Gly Lys Ala Asn Asp Ala Val Ile Ser Leu50 55 60Leu Ala Lys Ala Leu Ser Leu Pro Lys Arg Asp Val Thr Leu Ile Ala65 70 75 80Gly Glu Thr Ser Arg Lys Lys Lys Phe Leu Leu Pro Asn Arg Val Gln85 90 95Asp Ile Ile Phe Ser Leu His Ile Asp Val100 10521029211972212DNA213Chlamydia pneumoniae40029atgaaacaat tacttttctg tgtttgcgta tttgctatgt catgttctgc ttacgcatcc 60ccacgacgac aagatccttc tgttatgaag gaaacattcc gaaataatta tggcattatt 120gtttccggtc aagaatgggt aaagcgtggt tctgacggca ccatcaccaa agtactcaaa 180aatggagcta ccctgcatga agtttattct ggaggcctcc ttcatgggga aattacctta 240acgtttcccc ataccacagc attggacgtt gttcaaatct atgatcaagg tagactcgtt 300tctcgcaaaa ccttttttgt gaacggtctt ccatctcaag aagagctgtt caatgaagat 360ggcacgtttg tcctcacacg atggccggac aacaacgaca gtgataccat cacaaagcct 420tacttcatag aaacgacata tcaagggcat gtcatagaag gaagttatac ttcctttaat 480gggaaatact cctcatccat ccacaatgga gagggagttc gttctgtgtt ctcctccaat 540aacatccttc tttctgaaga gaccttcaat gaaggtgtca tggtgaaata taccacattc 600
tatccgaatc gcgatcccga atcgattact cattatcaaa atggacagcc tcacggctta 660cggctaacat atctacaagg tggcatcccc aatacgatag aggagtggcg ttatggcttt 720caagacggaa cgaccatcgt atttaaaaat ggttgtaaga catctgagat cgcttatgtt 780aagggagtga aagaaggttt agaactgcgc tacaatgaac aggaaattgt agctgaagaa 840gtttcttggc gtaatgattt tctgcatgga gaacgtaaga tctatgctgg aggaatccaa 900aagcatgaat ggtattaccg cgggagatct gtatctaaag ccaaattcga gcggctaaat 960gctgcaggat ag 97221030211323212PRT213Chlamydia pneumoniae40030Met Lys Gln Leu Leu Phe Cys Val Cys Val Phe Ala Met Ser Cys Ser1 5 10 15Ala Tyr Ala Ser Pro Arg Arg Gln Asp Pro Ser Val Met Lys Glu Thr20 25 30Phe Arg Asn Asn Tyr Gly Ile Ile Val Ser Gly Gln Glu Trp Val Lys35 40 45Arg Gly Ser Asp Gly Thr Ile Thr Lys Val Leu Lys Asn Gly Ala Thr50 55 60Leu His Glu Val Tyr Ser Gly Gly Leu Leu His Gly Glu Ile Thr Leu65 70 75 80Thr Phe Pro His Thr Thr Ala Leu Asp Val Val Gln Ile Tyr Asp Gln85 90 95Gly Arg Leu Val Ser Arg Lys Thr Phe Phe Val Asn Gly Leu Pro Ser100 105 110Gln Glu Glu Leu Phe Asn Glu Asp Gly Thr Phe Val Leu Thr Arg Trp115 120 125Pro Asp Asn Asn Asp Ser Asp Thr Ile Thr Lys Pro Tyr Phe Ile Glu130 135 140Thr Thr Tyr Gln Gly His Val Ile Glu Gly Ser Tyr Thr Ser Phe Asn145 150 155 160Gly Lys Tyr Ser Ser Ser Ile His Asn Gly Glu Gly Val Arg Ser Val165 170 175
Phe Ser Ser Asn Asn Ile Leu Leu Ser Glu Glu Thr Phe Asn Glu Gly180 185 190Val Met Val Lys Tyr Thr Thr Phe Tyr Pro Asn Arg Asp Pro Glu Ser195 200 205Ile Thr His Tyr Gln Asn Gly Gln Pro His Gly Leu Arg Leu Thr Tyr210 215 220Leu Gln Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly Phe225 230 235 240Gln Asp Gly Thr Thr Ile Val Phe Lys Asn Gly Cys Lys Thr Ser Glu245 250 255Ile Ala Tyr Val Lys Gly Val Lys Glu Gly Leu Glu Leu Arg Tyr Asn260 265 270Glu Gln Glu Ile Val Ala Glu Glu Val Ser Trp Arg Asn Asp Phe Leu275 280 285His Gly Glu Arg Lys Ile Tyr Ala Gly Gly Ile Gln Lys His Glu Trp290 295 300Tyr Tyr Arg Gly Arg Ser Val Ser Lys Ala Lys Phe Glu Arg Leu Asn305 310 315 320Ala Ala Gly21031211429212DNA213Chlamydia pneumoniae40031atgagtttag attttttcga ggagttctat catcagtcaa tactcaatac agggacgtcc 60ttccccgaag gatacttaaa tattgcagaa atactctctt atcctcattg cactgatgct 120aacactgact ttctctgtag ccagtctgac aacgatttta ttattgcaga atctaaagat 180aaactcacat tatttaacgc tgattttgct atttggctcg ttcctgagct tgttcaagga 240caggcagtca ctcggggata tattgcggtt tcccaaggag aaggaaacta tgaaccagaa 300atggctttcg aagcctctgg acaatacaat cagtcgtcgc tgattctcga agccctgcag 360ttatatctta aggatattaa agatactgaa aatgctctgc gttctttccg ctttaataac 420gatcactag 42921032
211142212PRT213Chlamydia pneumoniae40032Met Ser Leu Asp Phe Phe Glu Glu Phe Tyr His Gln Ser Ile Leu Asn1 5 10 15Thr Gly Thr Ser Phe Pro Glu Gly Tyr Leu Asn Ile Ala Glu Ile Leu20 25 30Ser Tyr Pro His Cys Thr Asp Ala Asn Thr Asp Phe Leu Cys Ser Gln35 40 45Ser Asp Asn Asp Phe Ile Ile Ala Glu Ser Lys Asp Lys Leu Thr Leu50 55 60Phe Asn Ala Asp Phe Ala Ile Trp Leu Val Pro Glu Leu Val Gln Gly65 70 75 80Gln Ala Val Thr Arg Gly Tyr Ile Ala Val Ser Gln Gly Glu Gly Asn85 90 95Tyr Glu Pro Glu Met Ala Phe Glu Ala Ser Gly Gln Tyr Asn Gln Ser100 105 110Ser Leu Ile Leu Glu Ala Leu Gln Leu Tyr Leu Lys Asp Ile Lys Asp115 120 125Thr Glu Asn Ala Leu Arg Ser Phe Arg Phe Asn Asn Asp His130 135 14021033211180212DNA213Chlamydia pneumoniae40033gtgtctattg ccttaaaccg agaagaagtt tgggataatc cccatcactt aatgtttatc 60ttaatgcaat tccaacaatt ttcaggggaa caggatcgtt ttggaagttt cttagaagca 120accatccgtg atcgggtctc ttttttagtc ttacaagaaa agattgccac tttaaagtag 1802103421159212PRT213Chlamydia pneumoniae40034Val Ser Ile Ala Leu Asn Arg Glu Glu Val Trp Asp Asn Pro His His1 5 10 15
Leu Met Phe Ile Leu Met Gln Phe Gln Gln Phe Ser Gly Glu Gln Asp20 25 30Arg Phe Gly Ser Phe Leu Glu Ala Thr Ile Arg Asp Arg Val Ser Phe35 40 45Leu Val Leu Gln Glu Lys Ile Ala Thr Leu Lys50 5521035211675212DNA213Chlamydia pneumoniae40035atgcaaaccc ttgctcgtct atttggccaa tctccatttg ctcctttaca agctcatctg 60gaaatggtgg tctcttgtgt ggaatacatg cttcctatat tcactgctct ccgagatgga 120agatatgaag aattattaga aatggcaaaa cttgtttctg ataaagagta tcaagcagat 180tgtataaaaa atgatatgag gaatcatctt cctgcaggat tattcatgcc gatatctcga 240gcggggattc tagaaattat ttctatacaa gatagcatcg cggatactgc tgaagatgtt 300gctatcttat taaccatcag acgattaaac ttttatccat ctatggaaac gctttttttc 360cgatttttgg aaaaaaatct agaagctttt gagttaacta tgacattgct acatgaattc 420aaccaattgc ttgaaagttc atttgggggg aggaaggcag ataaagcacg cttgcttgta 480gggcgtgtgg ctaaatctga acatgaatcg gatgttttgc aacgagaact tatgcaaata 540tttttttctg atgattttat aattcctgaa aaagagtttt atctttggtt acaagtaatt 600cgacgcactg cggggatttc agatagttct gaaaagctcg cacatagaat taatatgacc 660ctagaagaaa agtaa 67521036211224212PRT213Chlamydia pneumoniae40036Met Gln Thr Leu Ala Arg Leu Phe Gly Gln Ser Pro Phe Ala Pro Leu1 5 10 15Gln Ala His Leu Glu Met Val Val Ser Cys Val Glu Tyr Met Leu Pro20 25 30Ile Phe Thr Ala Leu Arg Asp Gly Arg Tyr Glu Glu Leu Leu Glu Met35 40 45Ala Lys Leu Val Ser Asp Lys Glu Tyr Gln Ala Asp Cys Ile Lys Asn50 55 60
Asp Met Arg Asn His Leu Pro Ala Gly Leu Phe Met Pro Ile Ser Arg65 70 75 80Ala Gly Ile Leu Glu Ile Ile Ser Ile Gln Asp Ser Ile Ala Asp Thr85 90 95Ala Glu Asp Val Ala Ile Leu Leu Thr Ile Arg Arg Leu Asn Phe Tyr100 105 110Pro Ser Met Glu Thr Leu Phe Phe Arg Phe Leu Glu Lys Asn Leu Glu115 120 125Ala Phe Glu Leu Thr Met Thr Leu Leu His Glu Phe Asn Gln Leu Leu130 135 140Glu Ser Ser Phe Gly Gly Arg Lys Ala Asp Lys Ala Arg Leu Leu Val145 150 155 160Gly Arg Val Ala Lys Ser Glu His Glu Ser Asp Val Leu Gln Arg Glu165 170 175Leu Met Gln Ile Phe Phe Ser Asp Asp Phe Ile Ile Pro Glu Lys Glu180 185 190Phe Tyr Leu Trp Leu Gln Val Ile Arg Arg Thr Ala Gly Ile Ser Asp195 200 205Ser Ser Glu Lys Leu Ala His Arg Ile Asn Met Thr Leu Glu Glu Lys210 215 220210372112538212DNA213Chlamydia pneumoniae40037atggcagtac gattaattgt tgatgaaggc cccttgtctg gtgtaatttt tgttctggaa 60gatgggataa gctggtctat aggacgcgac tctagtgcta atgacattcc tattgaagat 120cctaaactcg gtgcatcgca agccattatc aataagactg acggaagcta ctacatcaca 180aatttagatg atacaattcc tattgttgta aatggcgtag cgatccaaga aactacacag 240ttaaaaaatg aagatactat cttattagga agcaatcagt attctttctt atcagatgaa 300tttgatcctc aagatcttgt ttatgatttt gatattcccg aagaaaattt ttctaatgat 360tcaggggatt tgtccgatag taatgaacag ggaaaagatc ttgagcctcg gcaaacttcg 420gaaacaaatc attcaccgaa gcctaaggaa aagctgacca aagatcaggg aagtagcgat 480ccaattacaa gtggggatca ggagcttgct gatgcttttt tagcatcagc aaaagcggaa 540
aaaaatcaac caagagccaa agttgctaag aagggtttaa aagaatcttc aaacgagtct 600ttgaatccaa aggaacaaaa tgcaaaggat tctccgaaag gagaggaaag aaccaacaaa 660ccccagaacg ccattatgga agataacgga gcttcgccta ggcaagatcc gcaaccaaag 720tcagcagaac cctctcttaa aaacacagcc agggatgaga ctcccttgaa agaaaataaa 780cctgtagaag agaaggctaa taagaaagca acaccggatt ctccagaaaa aaaagatcaa 840cctgaggaag gttctaaaaa ggaaggctct aaaatagaag caacaccttt ggattcacaa 900aaagaatccg aggataagga agcagaagaa gcctttgtac aagaagaaga agagaacctt 960acggaagata ataaagaaga ttctgacagt gccgctgatg caaatgacga cacggcaagt 1020gaccatactg cagaggataa caaagaaact cctaaaaaag tcgagaacga aaagagcgca 1080gttctatccc catttcatgt tcaagactta tttcgattcg atcaaacaat ttttccagca 1140gagattgatg atattgcgaa aaaaaatatc tctgtagact tgacgcagcc ttctcgtttt 1200ttactcaaag ttttagccgg agctaatatt ggagcagagt tccatttaga ctcaggaaaa 1260acctatattt taggtacgga tcctacaact tgtgacatag tatttaatga cttaagtgtt 1320tctcatcaac atgctaaaat tactgtcggt aatgacgggg gcattcttat cgaggatctc 1380gatagtaaaa acggtgtcat tgttgaagga cgaaaaattg ataagacctc tacattgagc 1440tcgaatcaag ttgtggcttt aggaacgaca ttatttttac ttatagatca tcatgccccc 1500gctgatacta tagttgcttc tctatcccca gacgattaca gtttgtttgg gagacagcaa 1560gacgccgaag ccttagaaag acaagaggcc caagaagaag aagaaaaaca aaaacgcgct 1620acactacccg caggatcttt cattcttacc ctgtttgttg gaggattggc tattctcttt 1680ggtataggaa cagcttctct tttccatacc aaagaagtgg ttcctttaga aaatattgat 1740tatcaagaag atcttgccca ggttatcaat cagttcccta cggtgcgtta tacgtttaat 1800aaaacgaaca gccaactttt cttaatcgga catgtcaaaa atagtacgga caaaagcgag 1860ctgctgtata aagtagacgc cctttccttt gtgaaatccg tagatgataa tgttattgat 1920gatgaagctg tttggcagga gatgaacatc ctgttatcaa agcgacccga gtttaaaggc 1980atcagcatgc attccccaga acctgggaaa ttcatcatca caggctatgt caagactgag 2040gagcaagcag cttgcctcgt tgattattta aatatacatt ttaattacct ctcgttacta 2100gagaataaag ttgttgttga aacccagatg ttaaaagcaa ttgcaggcca tcttcttcaa 2160ggaggttttg caaacatcca tgtggccttt gtgaacggtg aagttatcct tactggttac 2220gtcaataacg atgatgcaga gaagttccgt gctgtagtgc aagagctgtc ggggattcct 2280ggtgtgaggt tggtcaagaa ttttgctgtc ttactcccag ctgaagaggg aatcatagat 2340ttaaacctac gttaccccaa tcgctatcgt gttacaggct attctagata cggagaaata 2400agtatcaatg tagttgtcaa tggcagaatc ctcacaagag gggacgtgat tgatggtatg 2460
acagtaacaa gtatacaacc taacgcgatc tttttagaga aggaagggtt gaaatataaa 2520atagactaca ataaataa253821038211845212PRT213Chlamydia pneumoniae40038Met Ala Val Arg Leu Ile Val Asp Glu Gly Pro Leu Ser Gly Val Ile1 5 10 15Phe Val Leu Glu Asp Gly Ile Ser Trp Ser Ile Gly Arg Asp Ser Ser20 25 30Ala Asn Asp Ile Pro Ile Glu Asp Pro Lys Leu Gly Ala Ser Gln Ala35 40 45Ile Ile Asn Lys Thr Asp Gly Ser Tyr Tyr Ile Thr Asn Leu Asp Asp50 55 60Thr Ile Pro Ile Val Val Asn Gly Val Ala Ile Gln Glu Thr Thr Gln65 70 75 80Leu Lys Asn Glu Asp Thr Ile Leu Leu Gly Ser Asn Gln Tyr Ser Phe85 90 95Leu Ser Asp Glu Phe Asp Pro Gln Asp Leu Val Tyr Asp Phe Asp Ile100 105 110Pro Glu Glu Asn Phe Ser Asn Asp Ser Gly Asp Leu Ser Asp Ser Asn115 120 125Glu Gln Gly Lys Asp Leu Glu Pro Arg Gln Thr Ser Glu Thr Asn His130 135 140Ser Pro Lys Pro Lys Glu Lys Leu Thr Lys Asp Gln Gly Ser Ser Asp145 150 155 160Pro Ile Thr Ser Gly Asp Gln Glu Leu Ala Asp Ala Phe Leu Ala Ser165 170 175Ala Lys Ala Glu Lys Asn Gln Pro Arg Ala Lys Val Ala Lys Lys Gly180 185 190Leu Lys Glu Ser Ser Asn Glu Ser Leu Asn Pro Lys Glu Gln Asn Ala195 200 205Lys Asp Ser Pro Lys Gly Glu Glu Arg Thr Asn Lys Pro Gln Asn Ala
210 215 220Ile Met Glu Asp Asn Gly Ala Ser Pro Arg Gln Asp Pro Gln Pro Lys225 230 235 240Ser Ala Glu Pro Ser Leu Lys Asn Thr Ala Arg Asp Glu Thr Pro Leu245 250 255Lys Glu Asn Lys Pro Val Glu Glu Lys Ala Asn Lys Lys Ala Thr Pro260 265 270Asp Ser Pro Glu Lys Lys Asp Gln Pro Glu Glu Gly Ser Lys Lys Glu275 280 285Gly Ser Lys Ile Glu Ala Thr Pro Leu Asp Ser Gln Lys Glu Ser Glu290 295 300Asp Lys Glu Ala Glu Glu Ala Phe Val Gln Glu Glu Glu Glu Asn Leu305 310 315 320Thr Glu Asp Asn Lys Glu Asp Ser Asp Ser Ala Ala Asp Ala Asn Asp325 330 335Asp Thr Ala Ser Asp His Thr Ala Glu Asp Asn Lys Glu Thr Pro Lys340 345 350Lys Val Glu Asn Glu Lys Ser Ala Val Leu Ser Pro Phe His Val Gln355 360 365Asp Leu Phe Arg Phe Asp Gln Thr Ile Phe Pro Ala Glu Ile Asp Asp370 375 380Ile Ala Lys Lys Asn Ile Ser Val Asp Leu Thr Gln Pro Ser Arg Phe385 390 395 400Leu Leu Lys Val Leu Ala Gly Ala Asn Ile Gly Ala Glu Phe His Leu405 410 415Asp Ser Gly Lys Thr Tyr Ile Leu Gly Thr Asp Pro Thr Thr Cys Asp420 425 430Ile Val Phe Asn Asp Leu Ser Val Ser His Gln His Ala Lys Ile Thr435 440 445Val Gly Asn Asp Gly Gly Ile Leu Ile Glu Asp Leu Asp Ser Lys Asn450 455 460Gly Val Ile Val Glu Gly Arg Lys Ile Asp Lys Thr Ser Thr Leu Ser465 470 475 480
Ser Asn Gln Val Val Ala Leu Gly Thr Thr Leu Phe Leu Leu Ile Asp485 490 495His His Ala Pro Ala Asp Thr Ile Val Ala Ser Leu Ser Pro Asp Asp500 505 510Tyr Ser Leu Phe Gly Arg Gln Gln Asp Ala Glu Ala Leu Glu Arg Gln515 520 525Glu Ala Gln Glu Glu Glu Glu Lys Gln Lys Arg Ala Thr Leu Pro Ala530 535 540Gly Ser Phe Ile Leu Thr Leu Phe Val Gly Gly Leu Ala Ile Leu Phe545 550 555 560Gly Ile Gly Thr Ala Ser Leu Phe His Thr Lys Glu Val Val Pro Leu565 570 575Glu Asn Ile Asp Tyr Gln Glu Asp Leu Ala Gln Val Ile Asn Gln Phe580 585 590Pro Thr Val Arg Tyr Thr Phe Asn Lys Thr Asn Ser Gln Leu Phe Leu595 600 605Ile Gly His Val Lys Asn Ser Thr Asp Lys Ser Glu Leu Leu Tyr Lys610 615 620Val Asp Ala Leu Ser Phe Val Lys Ser Val Asp Asp Asn Val Ile Asp625 630 635 640Asp Glu Ala Val Trp Gln Glu Met Asn Ile Leu Leu Ser Lys Arg Pro645 650 655Glu Phe Lys Gly Ile Ser Met His Ser Pro Glu Pro Gly Lys Phe Ile660 665 670Ile Thr Gly Tyr Val Lys Thr Glu Glu Gln Ala Ala Cys Leu Val Asp675 680 685Tyr Leu Asn Ile His Phe Asn Tyr Leu Ser Leu Leu Glu Asn Lys Val690 695 700Val Val Glu Thr Gln Met Leu Lys Ala Ile Ala Gly His Leu Leu Gln705 710 715 720Gly Gly Phe Ala Asn Ile His Val Ala Phe Val Asn Gly Glu Val Ile725 730 735
Leu Thr Gly Tyr Val Asn Asn Asp Asp Ala Glu Lys Phe Arg Ala Val740 745 750Val Gln Glu Leu Ser Gly Ile Pro Gly Val Arg Leu Val Lys Asn Phe755 760 765Ala Val Leu Leu Pro Ala Glu Glu Gly Ile Ile Asp Leu Asn Leu Arg770 775 780Tyr Pro Asn Arg Tyr Arg Val Thr Gly Tyr Ser Arg Tyr Gly Glu Ile785 790 795 800Ser Ile Asn Val Val Val Asn Gly Arg Ile Leu Thr Arg Gly Asp Val805 810 815Ile Asp Gly Met Thr Val Thr Ser Ile Gln Pro Asn Ala Ile Phe Leu820 825 830Glu Lys Glu Gly Leu Lys Tyr Lys Ile Asp Tyr Asn Lys835 840 84521039211237212DNA213Chlamydia pneumoniae40039atgaaagaat ttttagccta tatcattaag aatctagtgg accgccctga agaagtccgt 60attaaagaag ttcaggggac tcacacgatt atttatgaac taagtgtagc taaacctgat 120atcgggaaga tcattggcaa agaaggccgt acgatcaaag cgattcgtac tcttctggtt 180tctgtagcaa gcaggaacaa tgtaagggtc agtttagaaa ttatggaaga aaagtag 2372104021178212PRT213Chlamydia pneumoniae40040Met Lys Glu Phe Leu Ala Tyr Ile Ile Lys Asn Leu Val Asp Arg Pro1 5 10 15Glu Glu Val Arg Ile Lys Glu Val Gln Gly Thr His Thr Ile Ile Tyr20 25 30Glu Leu Ser Val Ala Lys Pro Asp Ile Gly Lys Ile Ile Gly Lys Glu35 40 45Gly Arg Thr Ile Lys Ala Ile Arg Thr Leu Leu Val Ser Val Ala Ser50 55 60
Arg Asn Asn Val Arg Val Ser Leu Glu Ile Met Glu Glu Lys65 70 7521041211228212DNA213Chlamydia pneumoniae40041atgtttttcg ctcctcttct ttatgaatcg ttacggaggg gtcttatgca tccaacatct 60catatgcaac agcaactcgc tcgcttggag tttatcaacg accagctgac tacagagtta 120gaacatgtaa atgaattatt atgtagttta ggtttccctg aaggtctcac tacaatcaag 180gcaatcgcag aggaagtcct ctctgatgac gaacctctac tagattag 2282104221175212PRT213Chlamydia pneumoniae40042Met Phe Phe Ala Pro Leu Leu Tyr Glu Ser Leu Arg Arg Gly Leu Met1 5 10 15His Pro Thr Ser His Met Gln Gln Gln Leu Ala Arg Leu Glu Phe Ile20 25 30Asn Asp Gln Leu Thr Thr Glu Leu Glu His Val Asn Glu Leu Leu Cys35 40 45Ser Leu Gly Phe Pro Glu Gly Leu Thr Thr Ile Lys Ala Ile Ala Glu50 55 60Glu Val Leu Ser Asp Asp Glu Pro Leu Leu Asp65 70 75210432111269212DNA213Chlamydia pneumoniae40043atgaaaaaac aggtatatca atggttagcg agtgtggttc ttttagcgct gacaatttca 60ggatacgctg aacttcctct ctcggaacaa aaagtaaaaa gtcacactta tacaacttta 120gacgaagtca aagactactt aagtaaacgg ggttttgtag aaacgcgaaa gcaagatggc 180gttttaagaa tagcaggaga tgttagagcc cggtggttgt atttcagaga agatatcaaa 240aacccctcag ataaagataa atacaatccc ttaccagtaa atcgttatcg tagtgaattt 300tatctctata ttgattatcg cgctgagagg aactggctgt cttcaaagat gaattggaca 360
gcaattgcag gaggggaaaa cactgcagct ggtgttgata tcaacagagc atttctagga 420tatcgttttt ataagaatcc cgaaacacgt acagatttct ttatggaaat cggacgttct 480ggtttaggag atctctttga gtcagaagtc caattccaaa gtaattttga cggactacat 540atatattgga ctcgagaact ttctaaggac tatccttatc aagtgattgt tcatggaggt 600cctttcgtcg tgaacatgac aaaaaaacat tatgcttggg ttgtagaagg gattctcaat 660cgtttgccta aacagttttt tgtgaaatgt agtgttgtcg actggaacac attcgttcct 720tcagaaacct ccactacaga aaaagctgct acaaacgcta tgaaatacaa atactgtgtt 780tggcagtggc tcgtcggaaa gcatagtcag gttccttgga tcaatggaca gaaaaagcct 840ctatatcttt atggagcttt cttaatgaac cctttagcaa aggctacgaa gactacgtta 900aatggaaaag aaaacctagc ttggtttatt ggaggaactt tagggggact cagaaaagct 960ggagactggt ctgccacagt acgttatgag tatgtcgaag ccttgtcggt tccagaaata 1020gatgtttcag ggattggccg tggtaattta ttaaagtttt ggttcgccca agcaattgct 1080gctaactatg atcctaaaga ggctaatggt tttacaaatt ataaaggatt ttccgctcta 1140tatatgtatg gcatcacaga ttctctatca ttcagagctt atggggctta ctccaaacca 1200gcaaacgata aactcggcag tgattttact ttccgaaagt ttgatctagg tataatttca 1260gcgttttaa 126921044211422212PRT213Chlamydia pneumoniae40044Met Lys Lys Gln Val Tyr Gln Trp Leu Ala Ser Val Val Leu Leu Ala1 5 10 15Leu Thr Ile Ser Gly Tyr Ala Glu Leu Pro Leu Ser Glu Gln Lys Val20 25 30Lys Ser His Thr Tyr Thr Thr Leu Asp Glu Val Lys Asp Tyr Leu Ser35 40 45Lys Arg Gly Phe Val Glu Thr Arg Lys Gln Asp Gly Val Leu Arg Ile50 55 60Ala Gly Asp Val Arg Ala Arg Trp Leu Tyr Phe Arg Glu Asp Ile Lys65 70 75 80Asn Pro Ser Asp Lys Asp Lys Tyr Asn Pro Leu Pro Val Asn Arg Tyr85 90 95
Arg Ser Glu Phe Tyr Leu Tyr Ile Asp Tyr Arg Ala Glu Arg Asn Trp100 105 110Leu Ser Ser Lys Met Asn Trp Thr Ala Ile Ala Gly Gly Glu Asn Thr115 120 125Ala Ala Gly Val Asp Ile Asn Arg Ala Phe Leu Gly Tyr Arg Phe Tyr130 135 140Lys Asn Pro Glu Thr Arg Thr Asp Phe Phe Met Glu Ile Gly Arg Ser145 150 155 160Gly Leu Gly Asp Leu Phe Glu Ser Glu Val Gln Phe Gln Ser Asn Phe165 170 175Asp Gly Leu His Ile Tyr Trp Thr Arg Glu Leu Ser Lys Asp Tyr Pro180 185 190Tyr Gln Val Ile Val His Gly Gly Pro Phe Val Val Asn Met Thr Lys195 200 205Lys His Tyr Ala Trp Val Val Glu Gly Ile Leu Asn Arg Leu Pro Lys210 215 220Gln Phe Phe Val Lys Cys Ser Val Val Asp Trp Asn Thr Phe Val Pro225 230 235 240Ser Glu Thr Ser Thr Thr Glu Lys Ala Ala Thr Asn Ala Met Lys Tyr245 250 255Lys Tyr Cys Val Trp Gln Trp Leu Val Gly Lys His Ser Gln Val Pro260 265 270Trp Ile Asn Gly Gln Lys Lys Pro Leu Tyr Leu Tyr Gly Ala Phe Leu275 280 285Met Asn Pro Leu Ala Lys Ala Thr Lys Thr Thr Leu Asn Gly Lys Glu290 295 300Asn Leu Ala Trp Phe Ile Gly Gly Thr Leu Gly Gly Leu Arg Lys Ala305 310 315 320Gly Asp Trp Ser Ala Thr Val Arg Tyr Glu Tyr Val Glu Ala Leu Ser325 330 335Val Pro Glu Ile Asp Val Ser Gly Ile Gly Arg Gly Asn Leu Leu Lys340 345 350Phe Trp Phe Ala Gln Ala Ile Ala Ala Asn Tyr Asp Pro Lys Glu Ala
355 360 365Asn Gly Phe Thr Asn Tyr Lys Gly Phe Ser Ala Leu Tyr Met Tyr Gly370 375 380Ile Thr Asp Ser Leu Ser Phe Arg Ala Tyr Gly Ala Tyr Ser Lys Pro385 390 395 400Ala Asn Asp Lys Leu Gly Ser Asp Phe Thr Phe Arg Lys Phe Asp Leu405 410 415Gly Ile Ile Ser Ala Phe420210452111596212DNA213Chlamydia pneumoniae40045atgttgggca aagaagaaga gtttacgtgt aaacaaaagc agtgtttgtc acattttgtt 60accaatctga cgtccgatgt atttgcttta aaaaatcttc cagaagtcgt taagggagct 120ttattttcta aatactcccg ttcagtttta ggtttgcgag cacttttgtt aaaagaattt 180ctatctaatg aagaggatgg agatgtttgt gacgaagcct atgacttcga aaccgatgta 240cagaaagctg cggactttta ccaaagggtt cttgataatt ttggggatga ttctgtagga 300gagcttggcg gagcccacct ggctatggaa aatgtctcta ttttggctgc taaagtttta 360gaggatgctc gaattggcgg atccccgcta gaaaagtcca caagatacgt ctatttcgat 420caaaaggtac ggggggagta tttatattac cgagacccta ttttgatgac ttcggccttt 480aaagacatgt ttttgggtac ttgtgatttt ttattcgata cctattctgc tttaatccct 540caagttcgtg cctattttga aaaactgtat cctaaagatt ctaaaacacc cgcatctgcc 600tatgccacat cattacgagc taaagtttta gattgtatac ggggacttct tcctgcggca 660actttgacaa atctaggatt tttcggtaac ggtaggtttt ggcaaaatct gattcacaag 720ttacaaggtc ataaccttgc agagttgcga cgtttaggag atgaatccct aacagagctt 780atgaaagtta ttccttcatt tgtaagtaga gccgagcctc atcatcacca tcatcaagct 840atgatgcaat atcgaagagc tttaaaagag cagctcaagg gacttgctga acaagcaaca 900tttagtgagg agatgtcttc ttcaccgagt gttcagttgg tatacggaga ccctgatggc 960atttataaag tagctgctgg atttcttttt ccttattcaa atcgttctct tacagatctc 1020atagactatt gtaaaaaaat gcctcatgaa gatcttgtac agattttaga gagcagtgtt 1080tctgcaagag aaaaccgccg gcataagtct cctcgtggtt tagaatgcgt agaatttggc 1140tttgatatac ttgctgattt cggtgcatac cgcgatttgc aacgacatcg gacgctgact 1200
caagaacgac agttactctc tacacatcat ggatacaatt ttcctgtgga gcttctagat 1260actcctatgg aaaaatctta tcgagaagct atggagaggg cgaatgaaac ctataatgag 1320attgttcagg agttccctga ggaagctcag tatatggttc ccatggctta caatatacgt 1380tggtttttcc atgtaaatgc tcgggctttg caatggattt gtgagttacg ctcacagcct 1440caaggtcatc aaaattaccg cactatagct acaggtttag tgcgagaggt tgtcaagttc 1500aatcctatgt acgaattatt tttcaaattt gtagattatt ctgacataga tttaggacgg 1560ttaaatcagg aaatgcgaaa agaaccaacg acctaa159621046211531212PRT213Chlamydia pneumoniae40046Met Leu Gly Lys Glu Glu Glu Phe Thr Cys Lys Gln Lys Gln Cys Leu1 5 10 15Ser His Phe Val Thr Asn Leu Thr Ser Asp Val Phe Ala Leu Lys Asn20 25 30Leu Pro Glu Val Val Lys Gly Ala Leu Phe Ser Lys Tyr Ser Arg Ser35 40 45Val Leu Gly Leu Arg Ala Leu Leu Leu Lys Glu Phe Leu Ser Asn Glu50 55 60Glu Asp Gly Asp Val Cys Asp Glu Ala Tyr Asp Phe Glu Thr Asp Val65 70 75 80Gln Lys Ala Ala Asp Phe Tyr Gln Arg Val Leu Asp Asn Phe Gly Asp85 90 95Asp Ser Val Gly Glu Leu Gly Gly Ala His Leu Ala Met Glu Asn Val100 105 110Ser Ile Leu Ala Ala Lys Val Leu Glu Asp Ala Arg Ile Gly Gly Ser115 120 125Pro Leu Glu Lys Ser Thr Arg Tyr Val Tyr Phe Asp Gln Lys Val Arg130 135 140Gly Glu Tyr Leu Tyr Tyr Arg Asp Pro Ile Leu Met Thr Ser Ala Phe145 150 155 160Lys Asp Met Phe Leu Gly Thr Cys Asp Phe Leu Phe Asp Thr Tyr Ser165 170 175
Ala Leu Ile Pro Gln Val Arg Ala Tyr Phe Glu Lys Leu Tyr Pro Lys180 185 190Asp Ser Lys Thr Pro Ala Ser Ala Tyr Ala Thr Ser Leu Arg Ala Lys195 200 205Val Leu Asp Cys Ile Arg G1y Leu Leu Pro Ala Ala Thr Leu Thr Asn210 215 220Leu Gly Phe Phe Gly Asn Gly Arg Phe Trp Gln Asn Leu Ile His Lys225 230 235 240Leu Gln Gly His Asn Leu Ala Glu Leu Arg Arg Leu Gly Asp Glu Ser245 250 255Leu Thr Glu Leu Met Lys Val Ile Pro Ser Phe Val Ser Arg Ala Glu260 265 270Pro His His His His His Gln Ala Met Met Gln Tyr Arg Arg Ala Leu275 280 285Lys Glu Gln Leu Lys Gly Leu Ala Glu Gln Ala Thr Phe Ser Glu Glu290 295 300Met Ser Ser Ser Pro Ser Val Gln Leu Val Tyr Gly Asp Pro Asp Gly305 310 315 320Ile Tyr Lys Val Ala Ala Gly Phe Leu Phe Pro Tyr Ser Asn Arg Ser325 330 335Leu Thr Asp Leu Ile Asp Tyr Cys Lys Lys Met Pro His Glu Asp Leu340 345 350Val Gln Ile Leu Glu Ser Ser Val Ser Ala Arg Glu Asn Arg Arg His355 360 365Lys Ser Pro Arg Gly Leu Glu Cys Val Glu Phe Gly phe Asp Ile Leu370 375 380Ala Asp Phe Gly Ala Tyr Arg Asp Leu Gln Arg His Arg Thr Leu Thr385 390 395 400Gln Glu Arg Gln Leu Leu Ser Thr His His Gly Tyr Asn Phe Pro Val405 410 415Glu Leu Leu Asp Thr Pro Met Glu Lys Ser Tyr Arg Glu Ala Met Glu420 425 430
Arg Ala Asn Glu Thr Tyr Asn Glu Ile Val Gln Glu Phe Pro Glu Glu435 440 445Ala Gln Tyr Met Val Pro Met Ala Tyr Asn Ile Arg Trp Phe Phe His450 455 460Val Asn Ala Arg Ala Leu Gln Trp Ile Cys Glu Leu Arg Ser Gln Pro465 470 475 480Gln Gly His Gln Asn Tyr Arg Thr Ile Ala Thr Gly Leu Val Arg Glu485 490 495Val Val Lys Phe Asn Pro Met Tyr Glu Leu Phe Phe Lys Phe Val Asp500 505 510Tyr Ser Asp Ile Asp Leu Gly Arg Leu Asn Gln Glu Met Arg Lys Glu515 520 525Pro Thr Thr530210472111206212DNA213Chlamydia pneumoniae40047atgttattgg taaggaaatg gttgcatact tgtttcaaat attggattta ctttcttccg 60gtggtaacgc tacttcttcc cctagtgtgt tacccttttc tgtcgattag tcaaaaaatt 120tatggatact ttgtttttac tacaatttct tctttaggct ggttttttgc attgagacgt 180agggaaaatc aattaaaaac agcagctgtt cagcttcttc aaacaaaaat tagaaaatta 240acagaaaata atgaagggtt aagacaaatt cgagaatctc ttaaagaaca tcagcaagag 300agtgctcaac tgcaaattca aagtcagaag cttaaaaaca gcctatttca tcttcagggt 360ttacttgtga aaactaaggg agaggggcaa aaattagaaa ctttgttact tcatagaaca 420gaagagaatc gatgtttgaa aatgcaagta gattctttaa ttcaggaatg cggagaaaaa 480acagaggaag tacaaacttt aaatcgagag ttggctgaga ctttagccta ccagcaagct 540ttaaatgacg agtatcaagc gaccttctct gagcaacgca atatgctgga taagcggcag 600atctacattg gaaagctgga aaacaaggtt caggatttaa tgtatgagat ccgtaacttg 660cttcagttag agtcagacat agcagagaat attccttctc aagaatcgaa tgctgttacg 720ggaaatattt ctttacaatt gtctagtgag ttaaaaaaaa ttgcttttaa ggctgaaaac 780atagaggcag cctcttcttt aacagcatca cgttaccttc atacagatac gagtgtgcat 840aactactctt tagagtgtcg ccagttattt gatagcttaa gagaagaaaa tctcgggatg 900ctttttgtct atgctcgtca atcccaacgt gcggtttttg ctaatgcgtt atttaaaacg 960
tggacggggt attgtgcaga agatttttta aaatttggta gtgacatagt gatttctggg 1020ggcaaacagt ggatggagga tcttcattcc tctagagaag aatgctctgg tagattagtg 1080attaaaacga aatcacgagg tcatcttcct ttccgttatt gtttaatggc tttgaataaa 1140ggccctcttt gctatcatgt tttgggggtt ctttatcctc tccataaaga agtgcttcag 1200agttga 120621048211401212PRT213Chlamydia pneumoniae40048Met Leu Leu Val Arg Lys Trp Leu His Thr Cys Phe Lys Tyr Trp Ile1 5 10 15Tyr Phe Leu Pro Val Val Thr Leu Leu Leu Pro Leu Val Cys Tyr Pro20 25 30Phe Leu Ser Ile Ser Gln Lys Ile Tyr Gly Tyr Phe Val Phe Thr Thr35 40 45Ile Ser Ser Leu Gly Trp Phe Phe Ala Leu Arg Arg Arg Glu Asn Gln50 55 60Leu Lys Thr Ala Ala Val Gln Leu Leu Gln Thr Lys Ile Arg Lys Leu65 70 75 80Thr Glu Asn Asn Glu Gly Leu Arg Gln Ile Arg Glu Ser Leu Lys Glu85 90 95His Gln Gln Glu Ser Ala Gln Leu Gln Ile Gln Ser Gln Lys Leu Lys100 105 110Asn Ser Leu Phe His Leu Gln Gly Leu Leu Val Lys Thr Lys Gly Glu115 120 125Gly Gln Lys Leu Glu Thr Leu Leu Leu His Arg Thr Glu Glu Asn Arg130 135 140Cys Leu Lys Met Gln Val Asp Ser Leu Ile Gln Glu Cys Gly Glu Lys145 150 155 160Thr Glu Glu Val Gln Thr Leu Asn Arg Glu Leu Ala Glu Thr Leu Ala165 170 175Tyr Gln Gln Ala Leu Asn Asp Glu Tyr Gln Ala Thr Phe Ser Glu Gln180 185 190
Arg Asn Met Leu Asp Lys Arg Gln Ile Tyr Ile Gly Lys Leu Glu Asn195 200 205Lys Val Gln Asp Leu Met Tyr Glu Ile Arg Asn Leu Leu Gln Leu Glu210 215 220Ser Asp Ile Ala Glu Asn Ile Pro Ser Gln Glu Ser Asn Ala Val Thr225 230 235 240Gly Asn Ile Ser Leu Gln Leu Ser Ser Glu Leu Lys Lys Ile Ala Phe245 250 255Lys Ala Glu Asn Ile Glu Ala Ala Ser Ser Leu Thr Ala Ser Arg Tyr260 265 270Leu His Thr Asp Thr Ser Val His Asn Tyr Ser Leu Glu Cys Arg Gln275 280 285Leu Phe Asp Ser Leu Arg Glu Glu Asn Leu Gly Met Leu Phe Val Tyr290 295 300Ala Arg Gln Ser Gln Arg Ala Val Phe Ala Asn Ala Leu Phe Lys Thr305 310 315 320Trp Thr Gly Tyr Cys Ala Glu Asp Phe Leu Lys Phe Gly Ser Asp Ile325 330 335Val Ile Ser Gly Gly Lys Gln Trp Met Glu Asp Leu His Ser Ser Arg340 345 350Glu Glu Cys Ser Gly Arg Leu Val Ile Lys Thr Lys Ser Arg Gly His355 360 365Leu Pro Phe Arg Tyr Cys Leu Met Ala Leu Asn Lys Gly Pro Leu Cys370 375 380Tyr His Val Leu Gly Val Leu Tyr Pro Leu His Lys Glu Val Leu Gln385 390 395 400Ser21049211675212DNA213Chlamydia pneumoniae40049atgacatcct ggatagaatt acttgataag caaattgaag atcaacatat gttaaagcac 60
gaattttatc agcgttggtc tgaaggaaag ttagaaaaac aacaacttca agcttatgcc 120aaagattact atttacatat taaagcattt ccttgttacc tttcagcgct gcatgctcgc 180tgtgatgact tgcagattcg tagacaaatt cttgagaatc tcatggatga agaagctgga 240aatcctaatc acatagattt atggagacag tttgctttat ctcttggagt ttctgaagag 300gagcttgcca atcatgaatt cagtcaggct gctcaagata tggtagcgac atttcgccgc 360ttatgcgaca tgccacaact tgccgtgggt ttaggcgctc tctatactta tgagattcag 420attcctcaag tctgtgtaga gaaaatccgt ggtttgaaag aatattttgg agtttctgct 480cgaggctatg catactttac tgtacatcaa gaagctgata ttaaacatgc cagcgaagag 540aaagaaatgc tacaaacttt ggtaggcaga gagaatcctg atgctgtttt gcaaggatca 600caagaagttt tagatactct atggaacttt ttgagctctt ttattaattc aacggagcct 660tgttcttgta agtag 67521050211224212PRT213Chlamydia pneumoniae40050Met Thr Ser Trp Ile Glu Leu Leu Asp Lys Gln Ile Glu Asp Gln His1 5 10 15Met Leu Lys His Glu Phe Tyr Gln Arg Trp Ser Glu Gly Lys Leu Glu20 25 30Lys Gln Gln Leu Gln Ala Tyr Ala Lys Asp Tyr Tyr Leu His Ile Lys35 40 45Ala Phe Pro Cys Tyr Leu Ser Ala Leu His Ala Arg Cys Asp Asp Leu50 55 60Gln Ile Arg Arg Gln Ile Leu Glu Asn Leu Met Asp Glu Glu Ala Gly65 70 75 80Asn Pro Asn His Ile Asp Leu Trp Arg Gln Phe Ala Leu Ser Leu Gly85 90 95Val Ser Glu Glu Glu Leu Ala Asn His Glu Phe Ser Gln Ala Ala Gln100 105 110Asp Met Val Ala Thr Phe Arg Arg Leu Cys Asp Met Pro Gln Leu Ala115 120 125Val Gly Leu Gly Ala Leu Tyr Thr Tyr Glu Ile Gln Ile Pro Gln Val130 135 140
Cys Val Glu Lys Ile Arg Gly Leu Lys Glu Tyr Phe Gly Val Ser Ala145 150 155 160Arg Gly Tyr Ala Tyr Phe Thr Val His Gln Glu Ala Asp Ile Lys His165 170 175Ala Ser Glu Glu Lys Glu Met Leu Gln Thr Leu Val Gly Arg Glu Asn180 185 190Pro Asp Ala Val Leu Gln Gly Ser Gln Glu Val Leu Asp Thr Leu Trp195 200 205Asn Phe Leu Ser Ser Phe Ile Asn Ser Thr Glu Pro Cys Ser Cys Lys210 215 220210512111287212DNA213Chlamydia pneumoniae40051atggatataa aaaaactctt ttgcttattt ctatgttctt ctctaattgc catgagtccc 60atttatggga aaacaggtga ctatgagaaa ctcaccctta cagggatcaa tatcattgat 120agaaacggcc tgtcagaaac tatttgctct aaagagaagc taaagaaata caccaaggta 180gactttcttg ctccccagcc ctatcaaaag gtcatgagga tgtataaaaa caaacgcgga 240gataacgttt cttgtttaac agcctatcac actaacgggc aaattaagca gtacctggag 300tgtctcaata atcgtgctta tggaagatat cgtgaatggc acgtcaacgg gaatatcaaa 360atccaagctg aggttatcgg aggtattgcg gatcttcatc cctcagcaga gtctggctgg 420ctatttgatc aaactacatt tgcctataat gatgaaggta tcttagaagc cgctatcgtc 480tatgaaaaag ggctgctcga aggatcttcg gtgtattacc atactaatgg gaatatttgg 540aaagagtgtc cctatcataa gggagttcct caaggtaaat tcctgacata cacatcttcg 600gggaaactgc tcaaagaaca gaattaccaa caaggcaaaa gacacggtct ttcgattcgc 660tacagcgaag attccgaaga agatgtttta gcctgggaag aatatcatga gggacgactc 720ctaaaagcag agtacttaga tcctcaaact cacgaaatct atgcgactat acacgaaggg 780aacggcattc aagcaatcta cggcaagtat gccgttatag aaactagggc attttaccga 840ggggaacctt atggaaaagt taccagattc gacaactccg gaacacagat tgtccaaacg 900tataaccttt tgcaaggcgc gaagcacgga gaagaatttt tcttttatcc tgagacaggg 960aaacccaagc tgcttcttaa ttggcatgaa ggaattttaa atgggatagt aaaaacttgg 1020tatcccggag gaaccttaga aagttgtaaa gaactcgtaa ataacaaaaa atccgggtta 1080ctgaccattt actaccctga aggacagatc atggcgaccg aagagtatga taatgatctt 1140
ctaattaaag gagagtactt ccgccctgga gaccgtcatc cctactctaa aatagatcgt 1200ggttgtggga ctgcagtatt tttctcgtcg gcgggaacta ttactaaaaa aatcccctat 1260caggacggca aacctttgct caactag 128721052211428212PRT213Chlamydia pneumoniae40052Met Asp Ile Lys Lys Leu Phe Cys Leu Phe Leu Cys Ser Ser Leu Ile1 5 10 15Ala Met Ser Pro Ile Tyr Gly Lys Thr Gly Asp Tyr Glu Lys Leu Thr20 25 30Leu Thr Gly Ile Asn Ile Ile Asp Arg Asn Gly Leu Ser Glu Thr Ile35 40 45Cys Ser Lys Glu Lys Leu Lys Lys Tyr Thr Lys Val Asp Phe Leu Ala50 55 60Pro Gln Pro Tyr Gln Lys Val Met Arg Met Tyr Lys Asn Lys Arg Gly65 70 75 80Asp Asn Val Ser Cys Leu Thr Ala Tyr His Thr Asn Gly Gln Ile Lys85 90 95Gln Tyr Leu Glu Cys Leu Asn Asn Arg Ala Tyr Gly Arg Tyr Arg Glu100 105 110Trp His Val Asn Gly Asn Ile Lys Ile Gln Ala Glu Val Ile Gly Gly115 120 125Ile Ala Asp Leu His Pro Ser Ala Glu Ser Gly Trp Leu Phe Asp Gln130 135 140Thr Thr Phe Ala Tyr Asn Asp Glu Gly Ile Leu Glu Ala Ala Ile Val145 150 155 160Tyr Glu Lys Gly Leu Leu Glu Gly Ser Ser Val Tyr Tyr His Thr Asn165 170 175Gly Asn Ile Trp Lys Glu Cys Pro Tyr His Lys Gly Val Pro Gln Gly180 185 190Lys Phe Leu Thr Tyr Thr Ser Ser Gly Lys Leu Leu Lys Glu Gln Asn195 200 205
Tyr Gln Gln Gly Lys Arg His Gly Leu Ser Ile Arg Tyr Ser Glu Asp210 215 220Ser Glu Glu Asp Val Leu Ala Trp Glu Glu Tyr His Glu Gly Arg Leu225 230 235 240Leu Lys Ala Glu Tyr Leu Asp Pro Gln Thr His Glu Ile Tyr Ala Thr245 250 255Ile His Glu Gly Asn Gly Ile Gln Ala Ile Tyr Gly Lys Tyr Ala Val260 265 270Ile Glu Thr Arg Ala Phe Tyr Arg Gly Glu Pro Tyr Gly Lys Val Thr275 280 285Arg Phe Asp Asn Ser Gly Thr Gln Ile Val Gln Thr Tyr Asn Leu Leu290 295 300Gln Gly Ala Lys His Gly Glu Glu Phe Phe Phe Tyr Pro Glu Thr Gly305 310 315 320Lys Pro Lys Leu Leu Leu Asn Trp His Glu Gly Ile Leu Asn Gly Ile325 330 335Val Lys Thr Trp Tyr Pro Gly Gly Thr Leu Glu Ser Cys Lys Glu Leu340 345 350Val Asn Asn Lys Lys Ser Gly Leu Leu Thr Ile Tyr Tyr Pro Glu Gly355 360 365Gln Ile Met Ala Thr Glu Glu Tyr Asp Asn Asp Leu Leu Ile Lys Gly370 375 380Glu Tyr Phe Arg Pro Gly Asp Arg His Pro Tyr Ser Lys Ile Asp Arg385 390 395 400Gly Cys Gly Thr Ala Val Phe Phe Ser Ser Ala Gly Thr Ile Thr Lys405 410 415Lys Ile Pro Tyr Gln Asp Gly Lys Pro Leu Leu Asn420 42521053211795212DNA213Chlamydia pneumoniae40053gtggaaaaac ttgagtttgt caccagcctt tcttctcctg atgatgattt gattactttc 60
aataaacagg gattgattgc aggcccagaa gaagaaaagg tagcgtttct tgtacgtagc 120aatgctatgc tagatgcagg acccgaaacc cccgcgtcgt ttcctgaatc tttaagggaa 180caattcgata ttttccctga gtatgttgaa gtgctctact ctaatgaagg attagatgtc 240tgggaagcag gatgtacgtg gattctaaat aatgaagtga ccatccaact gcgtaaacat 300caccggaaag cttcgcgatg gctaggaatg tattccagag atgaggtact cgctcacgaa 360gccgtgcatg ctgtgagaat gaaatttcat gagcctgtct ttgaagaggt gttagcttat 420caaacttctc gttggggttg gagaaggttt ttcggtcctc tatttcgctc tccaggagag 480agctacttgc tattattctt caccatttta ggtttaggaa tctccttatg gtatcctgcc 540ggtatactga ttatgctggt tttacctatg tattttttga tgcgattgtg catggcgcag 600agctatttgt atcgggccat gaaaaagatt cgtaaaatgc tcggagtacc tcccttatgg 660gtgctgctaa ggctgacgga taaggaaata aaaatgtttg ctaaagagcc tattcctgtt 720ttggaacact atgctagaaa acgaaagctt gaaaatgtcc gttggaagca aatttatcaa 780tcctactttg tttaa 79521054211264212PRT213Chlamydia pneumoniae40054Val Glu Lys Leu Glu Phe Val Thr Ser Leu Ser Ser Pro Asp Asp Asp1 5 10 15Leu Ile Thr Phe Asn Lys Gln Gly Leu Ile Ala Gly Pro Glu Glu Glu20 25 30Lys Val Ala Phe Leu Val Arg Ser Asn Ala Met Leu Asp Ala Gly Pro35 40 45Glu Thr Pro Ala Ser Phe Pro Glu Ser Leu Arg Glu Gln Phe Asp Ile50 55 60Phe Pro Glu Tyr Val Glu Val Leu Tyr Ser Asn Glu Gly Leu Asp Val65 70 75 80Trp Glu Ala Gly Cys Thr Trp Ile Leu Asn Asn Glu Val Thr Ile Gln85 90 95Leu Arg Lys His His Arg Lys Ala Ser Arg Trp Leu Gly Met Tyr Ser100 105 110Arg Asp Glu Val Leu Ala His Glu Ala Val His Ala Val Arg Met Lys115 120 125
Phe His Glu Pro Val Phe Glu Glu Val Leu Ala Tyr Gln Thr Ser Arg130 135 140Trp Gly Trp Arg Arg Phe Phe Gly Pro Leu Phe Arg Ser Pro Gly Glu145 150 155 160Ser Tyr Leu Leu Leu Phe Phe Thr Ile Leu Gly Leu Gly Ile Ser Leu165 170 175Trp Tyr Pro Ala Gly Ile Leu Ile Met Leu Val Leu Pro Met Tyr Phe180 185 190Leu Met Arg Leu Cys Met Ala Gln Ser Tyr Leu Tyr Arg Ala Met Lys195 200 205Lys Ile Arg Lys Met Leu Gly Val Pro Pro Leu Trp Val Leu Leu Arg210 215 220Leu Thr Asp Lys Glu Ile Lys Met Phe Ala Lys Glu Pro Ile Pro Val225 230 235 240Leu Glu His Tyr Ala Arg Lys Arg Lys Leu Glu Asn Val Arg Trp Lys245 250 255Gln Ile Tyr Gln Ser Tyr Phe Val26021055211234212DNA213Chlamydia pneumoniae40055atgaatgaag gtatccactc tgtctgtttt caaaaaacac ctcggcttac tgcgaagtcc 60gtagtgagta tggagatgct cttaactact caacagcttc cttccgcaga agggatgccc 120tcggttgcta atttggaagc ggatttttta cgagcagaag ctctgttagc agaaatgcga 180gaaattcgtg gttgcttgga gcaatctttg cgaacactag tccctagtga gtag2342105621177212PRT213Chlamydia pneumoniae40056Met Asn Glu Gly Ile His Ser Val Cys Phe Gln Lys Thr Pro Arg Leu1 5 10 15Thr Ala Lys Ser Val Val Ser Met Glu Met Leu Leu Thr Thr Gln Gln
20 25 30Leu Pro Ser Ala Glu Gly Met Pro Ser Val Ala Asn Leu Glu Ala Asp35 40 45Phe Leu Arg Ala Glu Ala Leu Leu Ala Glu Met Arg Glu Ile Arg Gly50 55 60Cys Leu Glu Gln Ser Leu Arg Thr Leu Val Pro Ser Glu65 70 75210572111815212DNA213Chlamydia pneumoniae40057atgaaacata cctttaccaa gcgtgttcta ttttttttct ttttagtgat tcccattccc 60ctactcctca atcttatggt cgtaggtttt ttctcatttt ctgccgctaa agcaaattta 120gtacaggtcc tccatacccg tgctacgaac ttaagtatag aattcgaaaa aaaactgacg 180atacacaagc ttttcctcga tagacttgcc aacacattag ccttaaaatc ctatgcatct 240ccttctgcag agccctatgc acaggcatac aatgagatga tggcactctc caatacagac 300ttttccttat gccttataga tccctttgat ggatctgtaa ggacgaaaaa tcctggagac 360cctttcattc gctatctaaa acagcatcct gaaatgaaga aaaagctatc cgcagctgta 420gggaaagcct ttttattgac cattccaggt aaaccacttt tacattatct tattctagtt 480gaagatgtcg catcttggga ttctacaacg acttcaggac tgcttgtaag tttctatccc 540atgtcttttt tacagaaaga tttattccaa tccttacaca tcaccaaagg aaatatctgc 600cttgtaaata agtatggcga ggtcctcttc tgtgctcagg acagtgaatc ttcttttgta 660ttttctctag atctccctaa tttaccgcaa ttccaagcaa gaagcccctc tgccatagaa 720attgagaaag cttctggaat tcttggtggg gagaacctaa tcacagtgag tatcaacaag 780aaacgctacc taggattggt actgaataaa attcctatcc aagggaccta cactctatct 840ttagttccag tttctgatct catccaatcc gccttgaaag ttcctctcaa tatttgtttt 900ttctatgtac ttgctttcct cctcatgtgg tggattttct ctaagatcaa caccaaactt 960aacaagcctc ttcaagaact gaccttctgt atggaagctg cctggcgagg aaaccataac 1020gtgaggtttg aaccccagcc ttacggttat gaattcaatg aactaggaaa tattttcaat 1080tgcactctcc tactcttatt gaattccatt gagaaagcag atatcgatta ccattcaggc 1140gaaaaattac aaaaagaatt agggatttta tcttcactac aaagtgcgtt actaagtccg 1200gatttcccta cgttccctaa agttaccttt agttcccaac atctccggag aaggcaactt 1260tccggtcatt ttaatggttg gacagttcaa gatggtggcg ataccctttt agggatcata 1320
gggctcgctg gcgatattgg tcttccttcc tatctctatg ctttatccgc acggagtctt 1380tttcttgcct atgcttcctc ggacgtttcg ttacaaaaaa tcagcaagga tactgccgac 1440agcttctcaa aaacaacaga aggcaatgag gctgtagttg ctatgacttt cattaaatat 1500gtagaaaaag atcgatctct agagctcctc tcgttaagcg agggagctcc taccatgttt 1560ctacaacgag gagaatcttt cgtacgtctc cccttagaga ctcaccaagc tctacagcct 1620ggagatcggt tgatctgcct cactggagga gaagacatcc tcaagtactt ttctcagctt 1680cctattgaag agctcttaaa agatccttta aaccctctaa atacagagaa tcttattgat 1740tctctaacca tgatgttaaa caacgaaacc gaacattctg cagatggaac tctgaccatc 1800ctttcatttt cataa 181521058211604212PRT213Chlamydia pneumoniae40058Met Lys His Thr Phe Thr Lys Arg Val Leu Phe Phe Phe Phe Leu Val1 5 10 15Ile Pro Ile Pro Leu Leu Leu Asn Leu Met Val Val Gly Phe Phe Ser20 25 30Phe Ser Ala Ala Lys Ala Asn Leu Val Gln Val Leu His Thr Arg Ala35 40 45Thr Asn Leu Ser Ile Glu Phe Glu Lys Lys Leu Thr Ile His Lys Leu50 55 60Phe Leu Asp Arg Leu Ala Asn Thr Leu Ala Leu Lys Ser Tyr Ala Ser65 70 75 80Pro Ser Ala Glu Pro Tyr Ala Gln Ala Tyr Asn Glu Met Met Ala Leu85 90 95Ser Asn Thr Asp Phe Ser Leu Cys Leu Ile Asp Pro Phe Asp Gly Ser100 105 110Val Arg Thr Lys Asn Pro Gly Asp Pro Phe Ile Arg Tyr Leu Lys Gln115 120 125His Pro Glu Met Lys Lys Lys Leu Ser Ala Ala Val Gly Lys Ala Phe130 135 140Leu Leu Thr Ile Pro Gly Lys Pro Leu Leu His Tyr Leu Ile Leu Val145 150 155 160
Glu Asp Val Ala Ser Trp Asp Ser Thr Thr Thr Ser Gly Leu Leu Val165 170 175Ser Phe Tyr Pro Met Ser Phe Leu Gln Lys Asp Leu Phe Gln Ser Leu180 185 190His Ile Thr Lys Gly Asn Ile Cys Leu Val Asn Lys Tyr Gly Glu Val195 200 205Leu Phe Cys Ala Gln Asp Ser Glu Ser Ser Phe Val Phe Ser Leu Asp210 215 220Leu Pro Asn Leu Pro Gln Phe Gln Ala Arg Ser Pro Ser Ala Ile Glu225 230 235 240Ile Glu Lys Ala Ser Gly Ile Leu Gly Gly Glu Asn Leu Ile Thr Val245 250 255Ser Ile Asn Lys Lys Arg Tyr Leu Gly Leu Val Leu Asn Lys Ile Pro260 265 270Ile Gln Gly Thr Tyr Thr Leu Ser Leu Val Pro Val Ser Asp Leu Ile275 280 285Gln Ser Ala Leu Lys Val Pro Leu Asn Ile Cys Phe Phe Tyr Val Leu290 295 300Ala Phe Leu Leu Met Trp Trp Ile Phe Ser Lys Ile Asn Thr Lys Leu305 310 315 320Asn Lys Pro Leu Gln Glu Leu Thr Phe Cys Met Glu Ala Ala Trp Arg325 330 335Gly Asn His Asn Val Arg Phe Glu Pro Gln Pro Tyr Gly Tyr Glu Phe340 345 350Asn Glu Leu Gly Asn Ile Phe Asn Cys Thr Leu Leu Leu Leu Leu Asn355 360 365Ser Ile Glu Lys Ala Asp Ile Asp Tyr His Ser Gly Glu Lys Leu Gln370 375 380Lys Glu Leu Gly Ile Leu Ser Ser Leu Gln Ser Ala Leu Leu Ser Pro385 390 395 400Asp Phe Pro Thr Phe Pro Lys Val Thr Phe Ser Ser Gln His Leu Arg405 410 415
Arg Arg Gln Leu Ser Gly His Phe Asn Gly Trp Thr Val Gln Asp Gly420 425 430Gly Asp Thr Leu Leu Gly Ile Ile Gly Leu Ala Gly Asp Ile Gly Leu435 440 445Pro Ser Tyr Leu Tyr Ala Leu Ser Ala Arg Ser Leu Phe Leu Ala Tyr450 455 460Ala Ser Ser Asp Val Ser Leu Gln Lys Ile Ser Lys Asp Thr Ala Asp465 470 475 480Ser Phe Ser Lys Thr Thr Glu Gly Asn Glu Ala Val Val Ala Met Thr485 490 495Phe Ile Lys Tyr Val Glu Lys Asp Arg Ser Leu Glu Leu Leu Ser Leu500 505 510Ser Glu Gly Ala Pro Thr Met Phe Leu Gln Arg Gly Glu Ser Phe Val515 520 525Arg Leu Pro Leu Glu Thr His Gln Ala Leu Gln Pro Gly Asp Arg Leu530 535 540Ile Cys Leu Thr Gly Gly Glu Asp Ile Leu Lys Tyr Phe Ser Gln Leu545 550 555 560Pro Ile Glu Glu Leu Leu Lys Asp Pro Leu Asn Pro Leu Asn Thr Glu565 570 575Asn Leu Ile Asp Ser Leu Thr Met Met Leu Asn Asn Glu Thr Glu His580 585 590Ser Ala Asp Gly Thr Leu Thr Ile Leu Ser Phe Ser595 600210592111170212DNA213Chlamydia pneumoniae40059atgcatccga aaatagaaaa aagaaatagc cttccactta cggcagtcgc tcctgtgttt 60gaagaatcgt atcatccttc tgtagctaca actgtagatt atgtagatgc cacgacactt 120tcccgacatc ttacagtctt aaaagatgtg ataaaagaag ctcgaaactt agatttaggg 180aaggcattcc tgacatctat gaaacagggt tttataaata cgggtacgga acttgccatt 240atacaagcat ctctggcaga tcagagtagt cgcgagtcgc gtaagaagga agagaagatc 300ttccatcagc acttaggaaa ggcagcccca caagcggcaa cagcaacttc aggagtgcag 360
cctactgcgg atcctgttgc tgataagatg cctttacaat ctgcatttgc ctatgttctc 420cttgataagt acattcctgc tcaagaggaa gccctttatg ctcttggaag ggagttaaac 480ctatcaggat atgcgcaaaa tttatttagt cctcttttag atatgattaa gagctttaac 540tctgctccta tcaactacaa tttaggatcg tacatatctc agacgagtgg cactgcgaat 600ttcgcgtatg gttatgagat gattttatcg cgctataaca acgaagtctc tcaatgtcgc 660ctggacatag caagtacagt aaaagctaaa gctgcgttag cgaacatgtc ggcttctgtt 720aaagcaaatg tgagtctgac tgatgcacag aagaaacaaa ttgaggatat cattgccagc 780tatacgaaat ctttagatgt gattcataca cagttaactg atgtgatgac aaatttagca 840tccataacct ttgttcctgg tttaaataaa tatgatcctt cgtatcgcat tgttggtggg 900gatttatcta tcattgcctt gcagaatgac gagaaggtac ttgtcgatgg taaggtggat 960atcacgactg ctgtgaatga aggaggccta cttaatttct tcactacagt ccttacggat 1020gtgcagaatt atggagactt agctcaaacg caacagctga tgttggactt agagcttaag 1080gcgatgcaac aacaatggag tttagtatct gcatctttga aattattgaa tgggatgtat 1140accacagtaa tttctggatt taaaaactaa 117021060211389212PRT213Chlamydia pneumoniae40060Met His Pro Lys Ile Glu Lys Arg Asn Ser Leu Pro Leu Thr Ala Val1 5 10 15Ala Pro Val Phe Glu Glu Ser Tyr His Pro Ser Val Ala Thr Thr Val20 25 30Asp Tyr Val Asp Ala Thr Thr Leu Ser Arg His Leu Thr Val Leu Lys35 40 45Asp Val Ile Lys Glu Ala Arg Asn Leu Asp Leu Gly Lys Ala Phe Leu50 55 60Thr Ser Met Lys Gln Gly Phe Ile Asn Thr Gly Thr Glu Leu Ala Ile65 70 75 80Ile Gln Ala Ser Leu Ala Asp Gln Ser Ser Arg Glu Ser Arg Lys Lys85 90 95Glu Glu Lys Ile Phe His Gln His Leu Gly Lys Ala Ala Pro Gln Ala100 105 110
Ala Thr Ala Thr Ser Gly Val Gln Pro Thr Ala Asp Pro Val Ala Asp115 120 125Lys Met Pro Leu Gln Ser Ala Phe Ala Tyr Val Leu Leu Asp Lys Tyr130 135 140Ile Pro Ala Gln Glu Glu Ala Leu Tyr Ala Leu Gly Arg Glu Leu Asn145 150 155 160Leu Ser Gly Tyr Ala Gln Asn Leu Phe Ser Pro Leu Leu Asp Met Ile165 170 175Lys Ser Phe Asn Ser Ala Pro Ile Asn Tyr Asn Leu Gly Ser Tyr Ile180 185 190Ser Gln Thr Ser Gly Thr Ala Asn Phe Ala Tyr Gly Tyr Glu Met Ile195 200 205Leu Ser Arg Tyr Asn Asn Glu Val Ser Gln Cys Arg Leu Asp Ile Ala210 215 220Ser Thr Val Lys Ala Lys Ala Ala Leu Ala Asn Met Ser Ala Ser Val225 230 235 240Lys Ala Asn Val Ser Leu Thr Asp Ala Gln Lys Lys Gln Ile Glu Asp245 250 255Ile Ile Ala Ser Tyr Thr Lys Ser Leu Asp Val Ile His Thr Gln Leu260 265 270Thr Asp Val Met Thr Asn Leu Ala Ser Ile Thr Phe Val Pro Gly Leu275 280 285Asn Lys Tyr Asp Pro Ser Tyr Arg Ile Val Gly Gly Asp Leu Ser Ile290 295 300Ile Ala Leu Gln Asn Asp Glu Lys Val Leu Val Asp Gly Lys Val Asp305 310 315 320Ile Thr Thr Ala Val Asn Glu Gly Gly Leu Leu Asn Phe Phe Thr Thr325 330 335Val Leu Thr Asp Val Gln Asn Tyr Gly Asp Leu Ala Gln Thr Gln Gln340 345 350Leu Met Leu Asp Leu Glu Leu Lys Ala Met Gln Gln Gln Trp Ser Leu355 360 365Val Ser Ala Ser Leu Lys Leu Leu Asn Gly Met Tyr Thr Thr Val Ile
370 375 380Ser Gly Phe Lys Asn38521061211537212DNA213Chlamydia pneumoniae40061gtgacaacac cacaatctcc tggttcgctt tctcaatctc accttcctca tccacatgat 60ccttgggata cagaacctac aagtcttccc gaagatccga atgataaggc aagccaagaa 120ctccattcgt tagttcatct ctttcgcaag ctatccattc acctacttag tgaagtcgaa 180aaaacagtac aacagctaaa acccgaccta ctagaactgg ctcttctcat ctgtgaaaaa 240tttctctata agaagctaga aaatcctcaa gaactggccc tgctcctctc taccgcgctc 300caaagacata cgacattaag atctctgact cccatcaaag tatttctcca tcccgaggat 360ctcaaaacac ttacagattg gatctccacc cacgaactcc ccatgattaa gcatgctgag 420tttttccctg acacttcttg tagacgatca ggattcaaaa tagaaacccc taacggaatt 480ctgagacaag aaatcagcga agaactagac catctacttt ctgttttgac agcatga 53721062211178212PRT213Chlamydia pneumoniae40062Val Thr Thr Pro Gln Ser Pro Gly Ser Leu Ser Gln Ser His Leu Pro1 5 10 15His Pro His Asp Pro Trp Asp Thr Glu Pro Thr Ser Leu Pro Glu Asp20 25 30Pro Asn Asp Lys Ala Ser Gln Glu Leu His Ser Leu Val His Leu Phe35 40 45Arg Lys Leu Ser Ile His Leu Leu Ser Glu Val Glu Lys Thr Val Gln50 55 60Gln Leu Lys Pro Asp Leu Leu Glu Leu Ala Leu Leu Ile Cys Glu Lys65 70 75 80Phe Leu Tyr Lys Lys Leu Glu Asn Pro Gln Glu Leu Ala Leu Leu Leu85 90 95Ser Thr Ala Leu Gln Arg His Thr Thr Leu Arg Ser Leu Thr Pro Ile100 105 110
Lys Val Phe Leu His Pro Glu Asp Leu Lys Thr Leu Thr Asp Trp Ile115 120 125Ser Thr His G1u Leu Pro Met Ile Lys His Ala Glu Phe Phe Pro Asp130 135 140Thr Ser Cys Arg Arg Ser Gly Phe Lys Ile Glu Thr Pro Asn Gly Ile145 150 155 160Leu Arg Gln Glu Ile Ser Glu Glu Leu Asp His Leu Leu Ser Val Leu165 170 175Thr Ala21063211798212DNA213Chlamydia pneumoniae40063atgcaaggtt ttttcccttt agcttctgga tccaaaggga attctgctta tctaggcacg 60gattcttgta agattcttat tgatttagga gtgagcaagc aagtcgtcac tcgggaatta 120ctctctatga atatcgatcc tgaagatatt caggcaattt ttgttacgca cgaacattct 180gatcatatct ccgggattaa aagttttgtt aaggcgtata acactcccat tgtttgcaac 240ttggagacgg ctcgtgcttt atgccatcta ctagatagcc atccagaatt caaaatattt 300tccacagggt cttcattttg ttttcaagat ctcgaagtac agacgttcaa tgtacctcat 360gatgctgtag atcctgtggc ttttattttt cattatcgcg aagagaaact gggtttttgc 420acagatttag gttgggtcac ctcttggatc acacatgaac tctatgattg tgattactta 480ttaattgagt ccaatcattc ccctgaattg gtacgtcaat ctcaacgtcc tgatgtttac 540aaaaagcgtg tattgagtaa attaggtcat atttctaacc aagagtgtgg tcagctttta 600caaaagatta tcactccgaa gttaaagaag ttatatcttg ctcacctgtc taccgagtgt 660aacactgcgg agctagcact ttctacagta tctgaatcga tagcttctat cacttctata 720gcaccagaaa ttgcattagc gcagggcatt acatctccaa tatacttttc tcgtcttgag 780gttgcatgtc cacggtaa79821064211265212PRT213Chlamydia pneumoniae40064Met Gln Gly Phe Phe Pro Leu Ala Ser Gly Ser Lys Gly Asn Ser Ala
1 5 10 15Tyr Leu Gly Thr Asp Ser Cys Lys Ile Leu Ile Asp Leu Gly Val Ser20 25 30Lys Gln Val Val Thr Arg Glu Leu Leu Ser Met Asn Ile Asp Pro Glu35 40 45Asp Ile Gln Ala Ile Phe Val Thr His Glu His Ser Asp His Ile Ser50 55 60Gly Ile Lys Ser Phe Val Lys Ala Tyr Asn Thr Pro Ile Val Cys Asn65 70 75 80Leu Glu Thr Ala Arg Ala Leu Cys His Leu Leu Asp Ser His Pro Glu85 90 95Phe Lys Ile Phe Ser Thr Gly Ser Ser Phe Cys Phe Gln Asp Leu Glu100 105 110Val Gln Thr Phe Asn Val Pro His Asp Ala Val Asp Pro Val Ala Phe115 120 125Ile Phe His Tyr Arg Glu Glu Lys Leu Gly Phe Cys Thr Asp Leu Gly130 135 140Trp Val Thr Ser Trp Ile Thr His Glu Leu Tyr Asp Cys Asp Tyr Leu145 150 155 160Leu Ile Glu Ser Asn His Ser Pro Glu Leu Val Arg Gln Ser Gln Arg165 170 175Pro Asp Val Tyr Lys Lys Arg Val Leu Ser Lys Leu Gly His Ile Ser180 185 190Asn Gln Glu Cys Gly Gln Leu Leu Gln Lys Ile Ile Thr Pro Lys Leu195 200 205Lys Lys Leu Tyr Leu Ala His Leu Ser Thr Glu Cys Asn Thr Ala Glu210 215 220Leu Ala Leu Ser Thr Val Ser Glu Ser Ile Ala Ser Ile Thr Ser Ile225 230 235 240Ala Pro Glu Ile Ala Leu Ala Gln Gly Ile Thr Ser Pro Ile Tyr Phe245 250 255Ser Arg Leu Glu Val Ala Cys Pro Arg260 265
21065211429212DNA213Chlamydia pneumoniae40065atggaagagt ttgtagcata tattgttaag aatttagtta ctaatcctga agctgttgaa 60attcgttcta ttgaagacga ggataacgaa tctattaagt tagaaattcg tgtcgcagca 120gaggatattg ggaaaattat tggaagaaga gggaacacaa tacatgcgct aagaacgatt 180cttagacgtg tatgttctag attaaaaaag aaagtgcaga tagatttggt tcagcctgaa 240aatggaactg atgtgattgc tgatcaagat tacatctgtg ataatgactc ttctaactct 300actgaagata ctttcggcga gtctgacacc tgctgcagtg gacactgtca ttacgacgaa 360gatcttaatc aagaagaaca agaagaaggc aatatgcatc attcttgcga atgtagtaac 420caccattaa 42921066211142212PRT213Chlamydia pneumoniae40066Met Glu Glu Phe Val Ala Tyr Ile Val Lys Asn Leu Val Thr Asn Pro1 5 10 15Glu Ala Val Glu Ile Arg Ser Ile Glu Asp Glu Asp Asn Glu Ser Ile20 25 30Lys Leu Glu Ile Arg Val Ala Ala Glu Asp Ile Gly Lys Ile Ile Gly35 40 45Arg Arg Gly Asn Thr Ile His Ala Leu Arg Thr Ile Leu Arg Arg Val50 55 60Cys Ser Arg Leu Lys Lys Lys Val Gln Ile Asp Leu Val Gln Pro Glu65 70 75 80Asn Gly Thr Asp Val Ile Ala Asp Gln Asp Tyr Ile Cys Asp Asn Asp85 90 95Ser Ser Asn Ser Thr Glu Asp Thr Phe Gly Glu Ser Asp Thr Cys Cys100 105 110Ser Gly His Cys His Tyr Asp Glu Asp Leu Asn Gln Glu Glu Gln Glu115 120 125Glu Gly Asn Met His His Ser Cys Glu Cys Ser Asn His His
130 135 14021067211471212DNA213Chlamydia pneumoniae40067atggaaaaac aaaatttaaa attagatgtc aaagagattg agtttcctga aacggtattc 60agccgtgata tcgaaactcg tgttatccaa gtaattattt tgcattgttt agcaaaaatt 120aacggtgttt ccctcctcgg aggaaatcta atagacgctc tgttcggtag agatatcgaa 180agaatgaagg ggatctatgt agaacaggat tcaaaaaatc atctggtcaa agttcgtgtc 240gaagtgaacg tagattacgg tgtttctata ccagagaaaa cagaagaaat ccagggatgc 300attgtttcag aaatttcaga atatacagga cttcatgtgg ccgctgtcca cgtgatcatt 360aaagggttga cacaaccaaa agatcgtatt gatgaagaaa ttgaagagga agtctctgtt 420caagatcttc cttcacctga agacttctta cttgagaatt ctgaagggta g 47121068211156212PRT213Chlamydia pneumoniae40068Met Glu Lys Gln Asn Leu Lys Leu Asp Val Lys Glu Ile Glu Phe Pro1 5 10 15Glu Thr Val Phe Ser Arg Asp Ile Glu Thr Arg Val Ile Gln Val Ile20 25 30Ile Leu His Cys Leu Ala Lys Ile Asn Gly Val Ser Leu Leu Gly Gly35 40 45Asn Leu Ile Asp Ala Leu Phe Gly Arg Asp Ile Glu Arg Met Lys Gly50 55 60Ile Tyr Val Glu Gln Asp Ser Lys Asn His Leu Val Lys Val Arg Val65 70 75 80Glu Val Asn Val Asp Tyr Gly Val Ser Ile Pro Glu Lys Thr Glu Glu85 90 95Ile Gln Gly Cys Ile Val Ser Glu Ile Ser Glu Tyr Thr Gly Leu His100 105 110Val Ala Ala Val His Val Ile Ile Lys Gly Leu Thr Gln Pro Lys Asp115 120 125
Arg Ile Asp Glu Glu Ile Glu Glu Glu Val Ser Val Gln Asp Leu Pro130 135 140Ser Pro Glu Asp Phe Leu Leu Glu Asn Ser Glu Gly145 150 15521069211231212DNA213Chlamydia pneumoniae40069gtgaaaaata aaattgttac attattagat cagctctacg aggatcagga gtcacgactt 60cagaagttag gagaggaaat tgttcctaac ctcactcctg aagatttatt gcaacctatg 120gattttcctc aattggaagg gaatccggca tttcgttttg aagagggtgt cttatcagga 180attggtgagg tgcgagctgc gattttagcg gcgctctctc aagagaacta g 2312107021176212PRT213Chlamydia pneumoniae40070Val Lys Asn Lys Ile Val Thr Leu Leu Asp Gln Leu Tyr Glu Asp Gln1 5 10 15Glu Ser Arg Leu Gln Lys Leu Gly Glu GLu Ile Val Pro Asn Leu Thr20 25 30Pro Glu Asp Leu Leu Gln Pro Met Asp Phe Pro Gln Leu Glu Gly Asn35 40 45Pro Ala Phe Arg Phe Glu Glu Gly Val Leu Ser Gly Ile Gly Glu Val50 55 60Arg Ala Ala Ile Leu Ala Ala Leu Ser Gln Glu Asn65 70 7521071211522212DNA213Chlamydia pneumoniae40071atgtggatca tagaccctct atcagcaaaa aaacctctac aagcagccat aaatgttcct 60ggcactccaa ttacaggagg acctaataca gcaactgctg acgatatcat tgcaaaattc 120tccaaagact caaaccctct gattgttact gtttactacg tctatcaatc cgtattagtt 180gctcaagata acctctccat cattgcccaa gaactccaag caaactcttc agctcaaacc 240tacctaaata accaagaagc cttataccaa tacgtcagta ttcctaaaaa taaactgaac 300
gataactcct ctagctatct acaaaacatc caatccgata accaagcgat aggagcttct 360cggcaagcta tccaaaacca aatttccagt ttaggaaacg cggctcaggt aatctccagt 420aacttgaaca caaataataa catcatccaa caatccttac aggtaggaca ggctcttatc 480cagaccttct ctcaaattgt aagcctaatt gctaacatct aa 52221072211173212PRT213Chlamydia pneumoniae40072Met Trp Ile Ile Asp Pro Leu Ser Ala Lys Lys Pro Leu Gln Ala Ala1 5 10 15Ile Asn Val Pro Gly Thr Pro Ile Thr Gly Gly Pro Asn Thr Ala Thr20 25 30Ala Asp Asp Ile Ile Ala Lys Phe Ser Lys Asp Ser Asn Pro Leu Ile35 40 45Val Thr Val Tyr Tyr Val Tyr Gln Ser Val Leu Val Ala Gln Asp Asn50 55 60Leu Ser Ile Ile Ala Gln Glu Leu Gln Ala Asn Ser Ser Ala Gln Thr65 70 75 80Tyr Leu Asn Asn Gln Glu Ala Leu Tyr Gln Tyr Val Ser Ile Pro Lys85 90 95Asn Lys Leu Asn Asp Asn Ser Ser Ser Tyr Leu Gln Asn Ile Gln Ser100 105 110Asp Asn Gln Ala Ile Gly Ala Ser Arg Gln Ala Ile Gln Asn Gln Ile115 120 125Ser Ser Leu Gly Asn Ala Ala Gln Val Ile Ser Ser Asn Leu Asn Thr130 135 140Asn Asn Asn Ile Ile Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile145 150 155 160Gln Thr Phe Ser Gln Ile Val Ser Leu Ile Ala Asn Ile165 17021073211222212DNA213Chlamydia pneumoniae
40073ttgtggtata aatctttagc tggagaggag aaggacgtgt ctgggaatga gtgcaatgac 60tatccagaag tttttaaaga tgacgtaagt gcttacgtat tggtaacttg tggtcagatg 120tcttctgaag gcaaaatcca ggtggagatg acttatgaag gagatccagc tgtgatcagc 180tatttattaa caaaagcacg agactcttta gatgagtctt aa 2222107421173212PRT213Chlamydia pneumoniae40074Leu Trp Tyr Lys Ser Leu Ala Gly Glu Glu Lys Asp Val Ser Gly Asn1 5 10 15Glu Cys Asn Asp Tyr Pro Glu Val Phe Lys Asp Asp Val Ser Ala Tyr20 25 30Val Leu Val Thr Cys Gly Gln Met Ser Ser Glu Gly Lys Ile Gln Val35 40 45Glu Met Thr Tyr Glu Gly Asp Pro Ala Val Ile Ser Tyr Leu Leu Thr50 55 60Lys Ala Arg Asp Ser Leu Asp Glu Ser65 70210752111494212DNA213Chlamydia pneumoniae40075atgaCtgtat cttaccaatc catatcgact cctcctcctg aaggagagtt tgatattttt 60gtagatggaa atgccactga agaagctgtt gttgctgcag aagtacaagt agctctccct 120gcaggagagc aatatgcgat gctgagagca acttccgagc tatgttttgg gattttaaca 180caatcggaat gtgctttgac acaagcactg cctcccaagg aaaaaccatt acaagaagag 240caatttctag taaaaaatgg catattaatg cgatcaacat ctctgccgaa cctaaaacca 300ggacaatcgc agcagactag cttagcttca catagaaatc ccttagcgca gcaatccaca 360tcttcgaatt ccacagggaa agcatcaaca gaaacaacct cgtcttcctt tccgtttttc 420tcatgcaaag ctcctgaagg agactccagt gtggacaaaa cgttcacagt gtctgtccaa 480acaccaaaag cacaagagca acaagaagca agcgcttctc aaagccaagc acaatttcat 540gtaaggtcct attctagcag caccataaaa gaacatagtg ctaaagaaaa agtctcacaa 600agcaccaagt cggcagagac acaaaaacac acacaaacta agtctgatgc gactcttagt 660
ccgatgtcac tctatagcac cttacataag gaagtacctc aagcactatc ctctacaaaa 720agccaacaaa aagatgaaga acatcgagat caaaggcaac aagaaggata cgagcaagaa 780caagagcaag aagaaggcaa gaaaaagaca ccatggtgca ctgtggaatc tctacaacag 840acttcaagtt cgaaccaggt gtacgagtct tatactccta ttattcctga tcctattgtg 900gagtttgctt tgtctgaatc acagctaagc gtgcttgcag gaaagcgtgt gaccaacctt 960gatgtcctta gaatatgtac agagctaatg aagttgatgc tcaaaagtag agctaacgac 1020acaatgacac gtcttgaaga aagagagctc atggaaaggg aagctcatga attggcggcg 1080agttattcac gtcaagctaa atacgcccgc tggctaggga tcgcgacagc aacgttaggt 1140attttaggag cgattgctcc tatggttgga gaaatttccg gagatagcat tttagggttt 1200gtccaaagga tttctggaag attcaaagat gcgactgcga aaaccttctt taaaggaata 1260ggaaaagttt tcacctctct atcacaactt actgaagccg cttctaaagt acatgagtta 1320tcagagagtg ctgtacgagc tgttgctgag tacagaaaag aagtcttcag aatgagacag 1380gatgaagtca cacgtaccat tgaggaagtc aaagacaact ggaaaagtat ggacaatttc 1440cttctgaaca ttctccaaac agaacatgac gctgctcgca gtctgtatca gtag149421076211497212PRT213Chlamydia pneumoniae40076Met Thr Val Ser Tyr Gln Ser Ile Ser Thr Pro Pro Pro Glu Gly Glu1 5 10 15Phe Asp Ile Phe Val Asp Gly Asn Ala Thr Glu Glu Ala Val Val Ala20 25 30Ala Glu Val Gln Val Ala Leu Pro Ala Gly Glu Gln Tyr Ala Met Leu35 40 45Arg Ala Thr Ser Glu Leu Cys Phe Gly Ile Leu Thr Gln Ser Glu Cys50 55 60Ala Leu Thr Gln Ala Leu Pro Pro Lys Glu Lys Pro Leu Gln Glu Glu65 70 75 80Gln Phe Leu Val Lys Asn Gly Ile Leu Met Arg Ser Thr Ser Leu Pro85 90 95Asn Leu Lys Pro Gly Gln Ser Gln Gln Thr Ser Leu Ala Ser His Arg100 105 110
Asn Pro Leu Ala Gln Gln Ser Thr Ser Ser Asn Ser Thr Gly Lys Ala115 120 125Ser Thr Glu Thr Thr Ser Ser Ser Phe Pro Phe Phe Ser Cys Lys Ala130 135 140Pro Glu Gly Asp Ser Ser Val Asp Lys Thr Phe Thr Val Ser Val Gln145 150 155 160Thr Pro Lys Ala Gln Glu Gln Gln Glu Ala Ser Ala Ser Gln Ser Gln165 170 175Ala Gln Phe His Val Arg Ser Tyr Ser Ser Ser Thr Ile Lys Glu His180 185 190Ser Ala Lys Glu Lys Val Ser Gln Ser Thr Lys Ser Ala Glu Thr Gln195 200 205Lys His Thr Gln Thr Lys Ser Asp Ala Thr Leu Ser Pro Met Ser Leu210 215 220Tyr Ser Thr Leu His Lys Glu Val Pro Gln Ala Leu Ser Ser Thr Lys225 230 235 240Ser Gln Gln Lys Asp Glu Glu His Arg Asp Gln Arg Gln Gln Glu Gly245 250 255Tyr Glu Gln Glu Gln Glu Gln Glu Glu Gly Lys Lys Lys Thr Pro Trp260 265 270Cys Thr Val Glu Ser Leu Gln Gln Thr Ser Ser Ser Asn Gln Val Tyr275 280 285Glu Ser Tyr Thr Pro Ile Ile Pro Asp Pro Ile Val Glu Phe Ala Leu290 295 300Ser Glu Ser Gln Leu Ser Val Leu Ala Gly Lys Arg Val Thr Asn Leu305 310 315 320Asp Val Leu Arg Ile Cys Thr Glu Leu Met Lys Leu Met Leu Lys Ser325 330 335Arg Ala Asn Asp Thr Met Thr Arg Leu Glu Glu Arg Glu Leu Met Glu340 345 350Arg Glu Ala His Glu Leu Ala Ala Ser Tyr Ser Arg Gln Ala Lys Tyr355 360 365Ala Arg Trp Leu Gly Ile Ala Thr Ala Thr Leu Gly Ile Leu Gly Ala
370 375 380Ile Ala Pro Met Val Gly Glu Ile Ser Gly Asp Ser Ile Leu Gly Phe385 390 395 400Val Gln Arg Ile Ser Gly Arg Phe Lys Asp Ala Thr Ala Lys Thr Phe405 410 415Phe Lys Gly Ile Gly Lys Val Phe Thr Ser Leu Ser Gln Leu Thr Glu420 425 430Ala Ala Ser Lys Val His Glu Leu Ser Glu Ser Ala Val Arg Ala Val435 440 445Ala Glu Tyr Arg Lys Glu Val Phe Arg Met Arg Gln Asp Glu Val Thr450 455 460Arg Thr Ile Glu Glu Val Lys Asp Asn Trp Lys Ser Met Asp Asn Phe465 470 475 480Leu Leu Asn Ile Leu Gln Thr Glu His Asp Ala Ala Arg Ser Leu Tyr485 490 495Gln210772111533212DNA213Chlamydia pneumoniae40077atgtcctctt ggttatctca agcaagtgag gttcttctca atcaagatcc ttatatccct 60gatgctccta ggtcacaaga atcttcggtt cctaagatta gctattctat cactgtagcg 120cctcaagaag ctcaaaagtc tcttcccaag tttttcactc agaaatttca gtcacaatgt 180aagtctgagc ctcctatcac ccatcataaa acattcatta ttgcaacacc aagagagaga 240atcttgcgtt tcggcagctc tttcgaatct caacttcaca acacatcgca agctcaaact 300tcatctcctt ggaatctttt ttctcaaaaa aatagcacag aagcaagtaa agctctgatg 360caagaactga ctatgccaaa atctccggag aaaacttcag agaaggctct agataaaaac 420ctgagttcta aacaagaagg ctcttgcaaa aattttgata cgctgcacct acaacaacat 480ctcaagttgt ttggaaccgt tgactcgcta tattctcaaa gcctagatag tgaacagcaa 540gaactcctcc aatcacgaag agaagagcgc agtgaaacct atgcaaacca gcagagttct 600gagaaaaaaa tagaaaccaa agttcagata aaagatctct gtaaagacct cttttctcag 660gatcaggatt ccaatcaaaa acaaaagaaa tcccctttcc aacaagatac atcacgtaaa 720
aatagaatag ccaaagcagc acaagctgtt cctgtaattc ctcctccaag cataggagtg 780tttacattga gctatctact cacaaaacaa gggattcttt cagacttttc ttcgtatgga 840tgccacaaag attccgtgga gtcgacacaa cgagagctcg atgctctaca tgaaaagaga 900atcgagacta tcaaggtcag catcgagaaa gaaaaacgag aaagattatg gggatctctt 960tctgacatta tcggttggct agctccgttt gtttctatag ggatcggcat tgttgctatc 1020ttgagtggag gcggtatctt tgcttttgca ggtttttttg cagggctaat ttctcttgtt 1080atcaagtgtt tagagaaact aaagttctgg gattggctag aaaaacatct gcctataaaa 1140aatgaagaac taagacgaaa aattataacc ataatccagt gggtcgtcta tttaacccct 1200gtcattctct ccatatgcac attaaaagta gaaaatttag gtttttcccc tattatagaa 1260ggagctatta aaggaatcca acccgcgata gaatctacga tggctgcttt aagatgcgct 1320atactgtttt ctcaagcaga aatctataaa ctcaaaggaa aactcactaa aatccaactc 1380gacattgaat tgaaaagttt tgatagggac gatcattacg aacgttctca agaactttta 1440gataacatgg agagttcttt cgaagccctt tcaagaatct taaattacat gcgtgaacta 1500gatcaagtgt atctccactc cttaagagga taa 153321078211510212PRT213Chlamydia pneumoniae40078Met Ser Ser Trp Leu Ser Gln Ala Ser Glu Val Leu Leu Asn Gln Asp1 5 10 15Pro Tyr Ile Pro Asp Ala Pro Arg Ser Gln Glu Ser Ser Val Pro Lys20 25 30Ile Ser Tyr Ser Ile Thr Val Ala Pro Gln Glu Ala Gln Lys Ser Leu35 40 45Pro Lys Phe Phe Thr Gln Lys Phe Gln Ser Gln Cys Lys Ser Glu Pro50 55 60Pro Ile Thr His His Lys Thr Phe Ile Ile Ala Thr Pro Arg Glu Arg65 70 75 80Ile Leu Arg Phe Gly Ser Ser Phe Glu Ser Gln Leu His Asn Thr Ser85 90 95Gln Ala Gln Thr Ser Ser Pro Trp Asn Leu Phe Ser Gln Lys Asn Ser100 105 110Thr Glu Ala Ser Lys Ala Leu Met Gln Glu Leu Thr Met Pro Lys Ser
115 120 125Pro Glu Lys Thr Ser Glu Lys Ala Leu Asp Lys Asn Leu Ser Ser Lys130 135 140Gln Glu Gly Ser Cys Lys Asn Phe Asp Thr Leu His Leu Gln Gln His145 150 155 160Leu Lys Leu Phe Gly Thr Val Asp Ser Leu Tyr Ser Gln Ser Leu Asp165 170 175Ser Glu Gln Gln Glu Leu Leu Gln Ser Arg Arg Glu Glu Arg Ser Glu180 185 190Thr Tyr Ala Asn Gln Gln Ser Ser Glu Lys Lys Ile Glu Thr Lys Val195 200 205Gln Ile Lys Asp Leu Cys Lys Asp Leu Phe Ser Gln Asp Gln Asp Ser210 215 220Asn Gln Lys Gln Lys Lys Ser Pro Phe Gln Gln Asp Thr Ser Arg Lys225 230 235 240Asn Arg Ile Ala Lys Ala Ala Gln Ala Val Pro Val Ile Pro Pro Pro245 250 255Ser Ile Gly Val Phe Thr Leu Ser Tyr Leu Leu Thr Lys Gln Gly Ile260 265 270Leu Ser Asp Phe Ser Ser Tyr Gly Cys His Lys Asp Ser Val Glu Ser275 280 285Thr Gln Arg Glu Leu Asp Ala Leu His Glu Lys Arg Ile Glu Thr Ile290 295 300Lys Val Ser Ile Glu Lys Glu Lys Arg Glu Arg Leu Trp Gly Ser Leu305 310 315 320Ser Asp Ile Ile Gly Trp Leu Ala Pro Phe Val Ser Ile Gly Ile Gly325 330 335Ile Val Ala Ile Leu Ser Gly Gly Gly Ile Phe Ala Phe Ala Gly Phe340 345 350Phe Ala Gly Leu Ile Ser Leu Val Ile Lys Cys Leu Glu Lys Leu Lys355 360 365Phe Trp Asp Trp Leu Glu Lys His Leu Pro Ile Lys Asn Glu Glu Leu370 375 380
Arg Arg Lys Ile Ile Thr Ile Ile Gln Trp Val Val Tyr Leu Thr Pro385 390 395 400Val Ile Leu Ser Ile Cys Thr Leu Lys Val Glu Asn Leu Gly Phe Ser405 410 415Pro Ile Ile Glu Gly Ala Ile Lys Gly Ile Gln Pro Ala Ile Glu Ser420 425 430Thr Met Ala Ala Leu Arg Cys Ala Ile Leu Phe Ser Gln Ala Glu Ile435 440 445Tyr Lys Leu Lys Gly Lys Leu Thr Lys Ile Gln Leu Asp Ile Glu Leu450 455 460Lys Ser Phe Asp Arg Asp Asp His Tyr Glu Arg Ser Gln Glu Leu Leu465 470 475 480Asp Asn Met Glu Ser Ser Phe Glu Ala Leu Ser Arg Ile Leu Asn Tyr485 490 495Met Arg Glu Leu Asp Gln Val Tyr Leu His Ser Leu Arg Gly500 505 51021079211588212DNA213Chlamydia pneumoniae40079atggcttacg gaactcgtta tcccacgcta gcattccata cagggggcat tggtgaatct 60gatgacggta tgcccccaca accctttgaa actttctgct acgattctgc tcttctacaa 120gcaaaaatcg aaaattttaa tatcgtccct tatacatctg tacttcctaa agagctcttt 180gggaatatcg ttcctgtaga tacctgtgta aaatctttca aacacggcgc tgtccttgaa 240gtaatcatgg cgggtcgtgg tgctgctctc tctgacggaa cccatgcgat cgccaccgga 300atcggcattt gctggggtaa agataaaaat ggagagctta ttggcggatg ggcagcagaa 360tatgttgaat tcttccctac atggataaat gacgaaattg cagaaaccca tgctaaaatg 420tggttgaaaa aatccctaca acacgaactt gacctacgct ctatagcaaa gcatagcgaa 480ttccaattct tccataatta catcaacatc aaacaaaaat tcggtttttg cttaactgca 540ttaggattcc taaatttcga aaatgctgaa ccagctaagg taaattaa 58821080211195212PRT213Chlamydia pneumoniae
40080Met Ala Tyr Gly Thr Arg Tyr Pro Thr Leu Ala Phe His Thr Gly Gly1 5 10 15Ile Gly Glu Ser Asp Asp Gly Met Pro Pro Gln Pro Phe Glu Thr Phe20 25 30Cys Tyr Asp Ser Ala Leu Leu Gln Ala Lys Ile Glu Asn Phe Asn Ile35 40 45Val Pro Tyr Thr Ser Val Leu Pro Lys Glu Leu Phe Gly Asn Ile Val50 55 60Pro Val Asp Thr Cys Val Lys Ser Phe Lys His Gly Ala Val Leu Glu65 70 75 80Val Ile Met Ala Gly Arg Gly Ala Ala Leu Ser Asp Gly Thr His Ala85 90 95Ile Ala Thr Gly Ile Gly Ile Cys Trp Gly Lys Asp Lys Asn Gly Glu100 105 110Leu Ile Gly Gly Trp Ala Ala Glu Tyr Val Glu Phe Phe Pro Thr Trp115 120 125Ile Asn Asp Glu Ile Ala Glu Thr His Ala Lys Met Trp Leu Lys Lys130 135 140Ser Leu Gln His Glu Leu Asp Leu Arg Ser Ile Ala Lys His Ser Glu145 150 155 160Phe Gln Phe Phe His Asn Tyr Ile Asn Ile Lys Gln Lys Phe Gly Phe165 170 175Cys Leu Thr Ala Leu Gly Phe Leu Asn Phe Glu Asn Ala Glu Pro Ala180 185 190Lys Val Asn195210812111092212DNA213Chlamydia pneumoniae40081atgaaattat atcagacctt gcgaggtatt gttttagtaa gtacgggatg catattctta 60ggaatgcacg gaggatatgc cgctgaggtt ccagtgactt catctgggta tgagaatctt 120
ttagaatcta aggaacagga tccttcaggt ctagcgatcc acgatcgcat tttgtttaag 180gtagatgaag agaatgtagt gactgcctta gatgtgatcc ataaattaaa cttactattt 240tataattcgt atcctcatct tatagattct ttccctgcac gatcccagta ctatactgcg 300atgtggcctg tggttcttga atctgtgatt gatgagtttt tgatggtggc agatgccaag 360gcaaagagaa tcgctacaga tcccaccgca gtgaatcaag aaatcgaaga gatgttcgga 420agagatCtct ctcctttgta tgcgcatttt gaaatgagtc ccaacgatat ttttaatgtg 480atcgatcgca ctttgacagc acaaagggtg atgggtatga tggtgcgctc taaggtaatg 540ttgaaggtga ctccagggaa aattcgagaa tattaccgaa agctagaaga agaagcctct 600aggaaagtca tctggaagta tcgtgtgttg acgattaaag ccaacacaga atccttggct 660agccagattg ctgataaagt gcgtgctcgt ctaaatgaag cgaaaacctg ggataaagat 720cgtttaactg ctcttgtgat ctctcaggga gggcaactcg tctgctccga agagttttct 780cgagagaata gtgagctctc ccaaagccat aagcaagagc tggacttgat tggctatcct 840aaagagctct gtgggttgcc taaggcacat aagtcaggat ataaactcta tatgttgtta 900gacaaaacct caggttctat agagccttta gatgttatgg agtccaagat caaacagcat 960ctttttgctt tagaagctga gagtgtagag aaacaatata aagacagatt acgcaagcgc 1020tacggctatg atgcttctat gattgcgaaa cttctttctg aagaagctcc acctctattt 1080tccttattat ag 109221082211363212PRT213Chlamydia pneumoniae40082Met Lys Leu Tyr Gln Thr Leu Arg Gly Ile Val Leu Val Ser Thr Gly1 5 10 15Cys Ile Phe Leu Gly Met His Gly Gly Tyr Ala Ala Glu Val Pro Val20 25 30Thr Ser Ser Gly Tyr Glu Asn Leu Leu Glu Ser Lys Glu Gln Asp Pro35 40 45Ser Gly Leu Ala Ile His Asp Arg Ile Leu Phe Lys Val Asp Glu Glu50 55 60Asn Val Val Thr Ala Leu Asp Val Ile His Lys Leu Asn Leu Leu Phe65 70 75 80Tyr Asn Ser Tyr Pro His Leu Ile Asp Ser Phe Pro Ala Arg Ser Gln85 90 95
Tyr Tyr Thr Ala Met Trp Pro Val Val Leu Glu Ser Val Ile Asp Glu100 105 110Phe Leu Met Val Ala Asp Ala Lys Ala Lys Arg Ile Ala Thr Asp Pro115 120 125Thr Ala Val Asn Gln Glu Ile Glu Glu Met Phe Gly Arg Asp Leu Ser130 135 140Pro Leu Tyr Ala His Phe Glu Met Ser Pro Asn Asp Ile Phe Asn Val145 150 155 160Ile Asp Arg Thr Leu Thr Ala Gln Arg Val Met Gly Met Met Val Arg165 170 175Ser Lys Val Met Leu Lys Val Thr Pro Gly Lys Ile Arg Glu Tyr Tyr180 185 190Arg Lys Leu Glu Glu Glu Ala Ser Arg Lys Val Ile Trp Lys Tyr Arg195 200 205Val Leu Thr Ile Lys Ala Asn Thr Glu Ser Leu Ala Ser Gln Ile Ala210 215 220Asp Lys Val Arg Ala Arg Leu Asn Glu Ala Lys Thr Trp Asp Lys Asp225 230 235 240Arg Leu Thr Ala Leu Val Ile Ser Gln Gly Gly Gln Leu Val Cys Ser245 250 255Glu Glu Phe Ser Arg Glu Asn Ser Glu Leu Ser Gln Ser His Lys Gln260 265 270Glu Leu Asp Leu Ile Gly Tyr Pro Lys Glu Leu Cys Gly Leu Pro Lys275 280 285Ala His Lys Ser Gly Tyr Lys Leu Tyr Met Leu Leu Asp Lys Thr Ser290 295 300Gly Ser Ile Glu Pro Leu Asp Val Met Glu Ser Lys Ile Lys Gln His305 310 315 320Leu Phe Ala Leu Glu Ala Glu Ser Val Glu Lys Gln Tyr Lys Asp Arg325 330 335Leu Arg Lys Arg Tyr Gly Tyr Asp Ala Ser Met Ile Ala Lys Leu Leu340 345 350
Ser Glu Glu Ala Pro Pro Leu Phe Ser Leu Leu355 360210832112076212DNA213Chlamydia trachomatis40083atgacgctct ttcattctca tcatgatgcc gtctctccag acagctacct atgttcttcc 60cttcagttag ttggtactgg cgtatacgaa ggagaaatcg agattcaaaa tatcccctct 120tatttccttg gattccaatt accctctcat tgcatacacc ttaatttaaa gagctctcta 180gctcaattag gaatagatgc ctcccttctt cactgcgaat tgagcaaaaa tcaacatcga 240gcacatatac atgctcaatt taccggtcat ggccccattg ctgaatctat gctagccctt 300ctccaaccag gagatcgtgt agcaaaacta tttgctgcag acgatcgcag actggtccga 360tctccagatt acctcgaaag catgctgaaa aatacagata aagctggcca tcctttgctc 420tgttttggga aaaaattaga acacttgatt tcttttgatg tggtagatga tcgccttgtc 480gtctcccttc ctaccctgcc gggagttgtt cgttatgatt cggatattta tggactcctt 540cctcttattc aaaaatcact cagtaatccc aaactcagca ttcgtcactt tttagctctg 600taccaacaga ttgtggaagg gcaacatgtc tcttgcggaa accatattct tctgatcaaa 660acagaaccgc tgcacatccg cactgtattt gctcgcgtgg taaatcaact cctccctcaa 720ggtctctccc acacttctgc caatattttg gaaccaacca ctcgagaatc cggggatatc 780tttgaatttt ttgggaaccc ttctgcacag atagaaagaa ttcctttaga atttttcact 840atcgaaccct ataaagaaca ttcttacttc tgtaatcggg atttattaca aaccatctta 900caatcagaaa gcgaaatcaa aaaaatattc gaaacagcgc ccaaagaacc tgtcaaagct 960gccacctatt tatcaaaagg cagtgaaatc tcttccctgc acacagactc ttggctcaca 1020ggatccgcag ctgcctatca atatagtgag caagcagata aaaacgagta cactcatgct 1080caaccttgct atcctttctt agaagcaatg gaaatgggcc tgatcaatag cgaaggagcc 1140ttactcactc gttatttccc ttcagctagc ttaaaaggaa tgttgatttc ctaccatgtg 1200cgccactatc tcaaacaaat ctactttcaa gttccctctt atacacatgg aaactatttc 1260tctcataatg acagaggttt gctattagat ctgcagcaag cagatattga tgttttctgg 1320gcagatgaag aaagcggccg tgtgttgcaa tatacaaaac gacgcgataa gaatagcggt 1380atgttcgtga tcaaaaatcg tgttgaagag tttcgatcag cttattttat tgctatttat 1440ggctctcgtc tccttgagaa taatttctct gctcagctcc ataccctcct agcgggctta 1500cagcaagcag cacatactct cggcattcct ggattctcaa agcctacccc acttgcagtc 1560atcaccggag gcggcactgg agttatggcc acaggaaatc gtgtagctaa agaactagga 1620atcctatctt gtggaaccgt tcttgattta gaagcttctc cagcacaaat cgaccaacct 1680
accaatgaat tcttagatgc taaaatgaca taccgcctac ctcaacttat agaaaggcaa 1740gaacactttt atgcagacct tcctatcctt gtagttggcg gtgtaggaac cgatttcgaa 1800ctctacctag aacttgtcta tctcaaaaca ggagctaaac caccgactcc cattttccta 1860attggaccta ttgaatactg gaaagaaaaa gtggcccacg cctacgagat caacctcaaa 1920gcaggaacca tccgtggatc cgaatggatc agcaactgcc tatattgtat cacttctccg 1980gaagctggaa ttgccgtatt cgaacaattc ctagctggag aactccctat aggatacgac 2040tatcctccag ctccagatgg attagtgatc gtctaa207621084211691212PRT213Chlamydia trachomatis40084Met Thr Leu Phe His Ser His His Asp Ala Val Ser Pro Asp Ser Tyr1 5 10 15Leu Cys Ser Ser Leu Gln Leu Val Gly Thr Gly Val Tyr Glu Gly Glu20 25 30Ile Glu Ile Gln Asn Ile Pro Ser Tyr Phe Leu Gly Phe Gln Leu Pro35 40 45Ser His Cys Ile His Leu Asn Leu Lys Ser Ser Leu Ala Gln Leu Gly50 55 60Ile Asp Ala Ser Leu Leu His Cys Glu Leu Ser Lys Asn Gln His Arg65 70 75 80Ala His Ile His Ala Gln Phe Thr Gly His Gly Pro Ile Ala Glu Ser85 90 95Met Leu Ala Leu Leu Gln Pro Gly Asp Arg Val Ala Lys Leu Phe Ala100 105 110Ala Asp Asp Arg Arg Leu Val Arg Ser Pro Asp Tyr Leu Glu Ser Met115 120 125Leu Lys Asn Thr Asp Lys Ala Gly His Pro Leu Leu Cys Phe Gly Lys130 135 140Lys Leu Glu His Leu Ile Ser Phe Asp Val Val Asp Asp Arg Leu Val145 150 155 160Val Ser Leu Pro Thr Leu Pro Gly Val Val Arg Tyr Asp Ser Asp Ile165 170 175
Tyr Gly Leu Leu Pro Leu Ile Gln Lys Ser Leu Ser Asn Pro Lys Leu180 185 190Ser Ile Arg His Phe Leu Ala Leu Tyr Gln Gln Ile Val Glu Gly Gln195 200 205His Val Ser Cys Gly Asn His Ile Leu Leu Ile Lys Thr Glu Pro Leu210 215 220His Ile Arg Thr Val Phe Ala Arg Val Val Asn Gln Leu Leu Pro Gln225 230 235 240Gly Leu Ser His Thr Ser Ala Asn Ile Leu Glu Pro Thr Thr Arg Glu245 250 255Ser Gly Asp Ile Phe Glu Phe Phe Gly Asn Pro Ser Ala Gln Ile Glu260 265 270Arg Ile Pro Leu Glu Phe Phe Thr Ile Glu Pro Tyr Lys Glu His Ser275 280 285Tyr Phe Cys Asn Arg Asp Leu Leu Gln Thr Ile Leu Gln Ser Glu Ser290 295 300Glu Ile Lys Lys Ile Phe Glu Thr Ala Pro Lys Glu Pro Val Lys Ala305 310 315 320Ala Thr Tyr Leu Ser Lys Gly Ser Glu Ile Ser Ser Leu His Thr Asp325 330 335Ser Trp Leu Thr Gly Ser Ala Ala Ala Tyr Gln Tyr Ser Glu Gln Ala340 345 350Asp Lys Asn Glu Tyr Thr His Ala Gln Pro Cys Tyr Pro Phe Leu Glu355 360 365Ala Met Glu Met Gly Leu Ile Asn Ser Glu Gly Ala Leu Leu Thr Arg370 375 380Tyr Phe Pro Ser Ala Ser Leu Lys Gly Met Leu Ile Ser Tyr His Val385 390 395 400Arg His Tyr Leu Lys Gln Ile Tyr Phe Gln Val Pro Ser Tyr Thr His405 410 415Gly Asn Tyr Phe Ser His Asn Asp Arg Gly Leu Leu Leu Asp Leu Gln420 425 430
Gln Ala Asp Ile Asp Val Phe Trp Ala Asp Glu Glu Ser Gly Arg Val435 440 445Leu Gln Tyr Thr Lys Arg Arg Asp Lys Asn Ser Gly Met Phe Val Ile450 455 460Lys Asn Arg Val Glu Glu Phe Arg Ser Ala Tyr Phe Ile Ala Ile Tyr465 470 475 480Gly Ser Arg Leu Leu Glu Asn Asn Phe Ser Ala Gln Leu His Thr Leu485 490 495Leu Ala Gly Leu Gln Gln Ala Ala His Thr Leu Gly Ile Pro Gly Phe500 505 510Ser Lys Pro Thr Pro Leu Ala Val Ile Thr Gly Gly Gly Thr Gly Val515 520 525Met Ala Thr Gly Asn Arg Val Ala Lys Glu Leu Gly Ile Leu Ser Cys530 535 540Gly Thr Val Leu Asp Leu Glu Ala Ser Pro Ala Gln Ile Asp Gln Pro545 550 555 560Thr Asn Glu Phe Leu Asp Ala Lys Met Thr Tyr Arg Leu Pro Gln Leu565 570 575Ile Glu Arg Gln Glu His Phe Tyr Ala Asp Leu Pro Ile Leu Val Val580 585 590Gly Gly Val Gly Thr Asp Phe Glu Leu Tyr Leu Glu Leu Val Tyr Leu595 600 605Lys Thr Gly Ala Lys Pro Pro Thr Pro Ile Phe Leu Ile Gly Pro Ile610 615 620Glu Tyr Trp Lys Glu Lys Val Ala His Ala Tyr Glu Ile Asn Leu Lys625 630 635 640Ala Gly Thr Ile Arg Gly Ser Glu Trp Ile Ser Asn Cys Leu Tyr Cys645 650 655Ile Thr Ser Pro Glu Ala Gly Ile Ala Val Phe Glu Gln Phe Leu Ala660 665 670Gly Glu Leu Pro Ile Gly Tyr Asp Tyr Pro Pro Ala Pro Asp Gly Leu675 680 685
Val Ile Val69021085211966212DNA213Chlamydia trachomatis40085atgaagcgtt tattttttat ctgcgccctc gccctttctc ctctagcata tggagctgtt 60caaaaggatc ctatgttaat gaaggagact ttccgtaata actacgggat cattgtctct 120aagcaagaat ggaacaaacg tggatgcgat ggctccatca ctagagtatt caaagatgga 180actacaacct tagaagttta tgcgcaaggt gctttacatg gggaagtcac acgaacgttt 240cctcactcta ctaccctggc cgttatagaa acttatgatc agggaaggct tctttctaag 300aagaccttct tcccaaatgc tttgcctgct aaagaagaag tttaccacga agatgggtct 360ttctccctaa cacgttggcc tgacaataac aactctgaca caatcacaga cccctgcttt 420gtagaaaaaa cttatggggg aagagtattg gaaggtcatt acacctcttt taatggaaaa 480tactcttcaa caatccttaa cggcgaggga gttcgctcta ctttttcttc ggatagtatc 540ttgttgacag aagagtcgtt taatgatggc gtaatggtca aaaaaacgac attttactcg 600actcgagaac ccgaaaccgt cactcattat gtcaatgggt accctcacgg agttcggttt 660acctatcttc ctggtgggat tccaaatacg attgaagaat ggcgatatgg acatcaagac 720ggccttacaa tcttatttaa aaatggttgt aagattgctg aagtcccatt tgtacgcgga 780gcaaaaaatg gaatcgaact ccgatacaat gaacaagaga atatcgctga agagatttct 840tggcagcaca acatcttgca tggagtccgt aaaatccatg cggcgggggt atgcaaatcc 900gaatggtatt acaaaggcaa acctgtctcg caaatcaagt ttgaacgact cagcgctgcc 960agataa 96621086211321212PRT213Chlamydia trachomatis40086Met Lys Arg Leu Phe Phe Ile Cys Ala Leu Ala Leu Ser Pro Leu Ala1 5 10 15Tyr Gly Ala Val Gln Lys Asp Pro Met Leu Met Lys Glu Thr Phe Arg20 25 30Asn Asn Tyr Gly Ile Ile Val Ser Lys Gln Glu Trp Asn Lys Arg Gly35 40 45Cys Asp Gly Ser Ile Thr Arg Val Phe Lys Asp Gly Thr Thr Thr Leu
50 55 60Glu Val Tyr Ala Gln Gly Ala Leu His Gly Glu Val Thr Arg Thr Phe65 70 75 80Pro His Ser Thr Thr Leu Ala Val Ile Glu Thr Tyr Asp Gln Gly Arg85 90 95Leu Leu Ser Lys Lys Thr Phe Phe Pro Asn Ala Leu Pro Ala Lys Glu100 105 110Glu Val Tyr His Glu Asp Gly Ser Phe Ser Leu Thr Arg Trp Pro Asp115 120 125Asn Asn Asn Ser Asp Thr Ile Thr Asp Pro Cys Phe Val Glu Lys Thr130 135 140Tyr Gly Gly Arg Val Leu Glu Gly His Tyr Thr Ser Phe Asn Gly Lys145 150 155 160Tyr Ser Ser Thr Ile Leu Asn Gly Glu Gly Val Arg Ser Thr Phe Ser165 170 175Ser Asp Ser Ile Leu Leu Thr Glu Glu Ser Phe Asn Asp Gly Val Met180 185 190Val Lys Lys Thr Thr Phe Tyr Ser Thr Arg Glu Pro Glu Thr Val Thr195 200 205His Tyr Val Asn Gly Tyr Pro His Gly Val Arg Phe Thr Tyr Leu Pro210 215 220Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly His Gln Asp225 230 235 240Gly Leu Thr Ile Leu Phe Lys Asn Gly Cys Lys Ile Ala Glu Val Pro245 250 255Phe Val Arg Gly Ala Lys Asn Gly Ile Glu Leu Arg Tyr Asn Glu Gln260 265 270Glu Asn Ile Ala Glu Glu Ile Ser Trp Gln His Asn Ile Leu His Gly275 280 285Val Arg Lys Ile His Ala Ala Gly Val Cys Lys Ser Glu Trp Tyr Tyr290 295 300Lys Gly Lys Pro Val Ser Gln Ile Lys Phe Glu Arg Leu Ser Ala Ala305 310 315 320
Arg21087211426212DNA213Chlamydia trachomatis40087gtgagtttag attttttaga ggattttttc cgtcgctcaa ttaccaatca caacacagct 60tttccagaag gctttctgga tatatctgat gtcttagctc gttcggcttt agattttaag 120gctgaggaac ttgctgacag tgctgttaat gacttcatcg tatcagaatc ttcagataaa 180ctcactttat ttaacacaaa ttttgctgtg tggttggtac ctacattagt tgatggtgag 240gcaattactc gcggctacat cgctttaaac cagggtgaag agttctctcc tgaattagct 300tttgaagcat caggaaagta taataattcg agtttaatct tagaggcgtt gcgaagatat 360ttgtgtgata tccaggatac agaaaaagaa ttacgggcat tacgcccgcc ttcaatagat 420ggatag 42621088211141212PRT213Chlamydia trachomatis40088Val Ser Leu Asp Phe Leu Glu Asp Phe Phe Arg Arg Ser Ile Thr Asn1 5 10 15His Asn Thr Ala Phe Pro Glu Gly Phe Leu Asp Ile Ser Asp Val Leu20 25 30Ala Arg Ser Ala Leu Asp Phe Lys Ala Glu Glu Leu Ala Asp Ser Ala35 40 45Val Asn Asp Phe Ile Val Ser Glu Ser Ser Asp Lys Leu Thr Leu Phe50 55 60Asn Thr Asn Phe Ala Val Trp Leu Val Pro Thr Leu Val Asp Gly Glu65 70 75 80Ala Ile Thr Arg Gly Tyr Ile Ala Leu Asn Gln Gly Glu Glu Phe Ser85 90 95Pro Glu Leu Ala Phe Glu Ala Ser Gly Lys Tyr Asn Asn Ser Ser Leu100 105 110Ile Leu Glu Ala Leu Arg Arg Tyr Leu Cys Asp Ile Gln Asp Thr Glu
115 120 125Lys Glu Leu Arg Ala Leu Arg Pro Pro Ser Ile Asp Gly130 135 14021089211240212DNA213Chlamydia trachomatis40089ttggaagata gaatgatcga cgggattcag acgtgttctt ttagccctac gcatcgctta 60actgcgaaat ccgcagtgag tatagagatg cctttagcaa cacaaaatct tcaagaggga 120gctttggtca atgcaaagct cgaagcggat ttcgcgagag cagagcagat tcttacagag 180atgcaagaaa tccgttctag tttagagagg tctttagaga ctctctttcc ccgcgagtaa 2402109021179212PRT213Chlamydia trachomatis40090Leu Glu Asp Arg Met Ile Asp Gly Ile Gln Thr Cys Ser Phe Ser Pro1 5 10 15Thr His Arg Leu Thr Ala Lys Ser Ala Val Ser Ile Glu Met Pro Leu20 25 30Ala Thr Gln Asn Leu Gln Glu Gly Ala Leu Val Asn Ala Lys Leu Glu35 40 45Ala Asp Phe Ala Arg Ala Glu Gln Ile Leu Thr Glu Met Gln Glu Ile50 55 60Arg Ser Ser Leu Glu Arg Ser Leu Glu Thr Leu Phe Pro Arg Glu65 70 7521091211696212DNA213Chlamydia trachomatis40091atgatggagg tgtttatgaa ttttttagat cagttagatt taattattca aaataagcat 60atgctagaac acacatttta tgtgaaatgg tcgaaggggg agcttactaa agagcaatta 120caggcgtatg ccaaagacta ttatttacat atcaaagcct ttcctaaata tttatctgcg 180attcatagtc gttgcgatga tttagaggcg cgtaagttat tgttagataa cttgatggat 240gaagagaacg gttaccctaa tcatattgat ttgtggaagc agtttgtgtt tgctctagga 300
gttactccag aagagttaga ggctcatgag cctagtgaag cagcaaaagc gaaagtagct 360actttcatgc ggtggtgtac aggagattct ttagctgcag gagtggctgc tttgtattct 420tatgagagtc aaattccacg tatcgctaga gagaaaattc gtggattgac tgagtacttt 480ggattttcca atcctgaaga ctatgcatat ttcacagaac atgaagaagc ggatgtgcgg 540catgctagag aagaaaaagc gctcattgag atgcttctca aagatgacgc tgataaagtg 600ttagaggcat cgcaagaagt aacgcaatct ttgtatggct ttttagattc ttttttggat 660ccaggaactt gttgtagttg tcatcaatct tattaa69621092211231212PRT213Chlamydia trachomatis40092Met Met Glu Val Phe Met Asn Phe Leu Asp Gln Leu Asp Leu Ile Ile1 5 10 15Gln Asn Lys His Met Leu Glu His Thr Phe Tyr Val Lys Trp Ser Lys20 25 30Gly Glu Leu Thr Lys Glu Gln Leu Gln Ala Tyr Ala Lys Asp Tyr Tyr35 40 45Leu His Ile Lys Ala Phe Pro Lys Tyr Leu Ser Ala Ile His Ser Arg50 55 60Cys Asp Asp Leu Glu Ala Arg Lys Leu Leu Leu Asp Asn Leu Met Asp65 70 75 80Glu Glu Asn Gly Tyr Pro Asn His Ile Asp Leu Trp Lys Gln Phe Val85 90 95Phe Ala Leu Gly Val Thr Pro Glu Glu Leu Glu Ala His Glu Pro Ser100 105 110Glu Ala Ala Lys Ala Lys Val Ala Thr Phe Met Arg Trp Cys Thr Gly115 120 125Asp Ser Leu Ala Ala Gly Val Ala Ala Leu Tyr Ser Tyr Glu Ser Gln130 135 140Ile Pro Arg Ile Ala Arg Glu Lys Ile Arg Gly Leu Thr Glu Tyr Phe145 150 155 160Gly Phe Ser Asn Pro Glu Asp Tyr Ala Tyr Phe Thr Glu His Glu Glu165 170 175
Ala Asp Val Arg His Ala Arg Glu Glu Lys Ala Leu Ile Glu Met Leu180 185 190Leu Lys Asp Asp Ala Asp Lys Val Leu Glu Ala Ser Gln Glu Val Thr195 200 205Gln Ser Leu Tyr Gly Phe Leu Asp Ser Phe Leu Asp Pro Gly Thr Cys210 215 220Cys Ser Cys His Gln Ser Tyr225 23021093211816212DNA213Chlamydia trachomatis40093ttgagagcag ccttgaatac acttgaattt ctttcttctc ccccctcttc ggatccttac 60gatgatttat tacaattaaa caaagaagga ttccttgctg gccctgaaga agaaaaacaa 120gctttttttc ttcgggtaga aaggacatta gcagaagctc ctgtacatcc cacccctttt 180cccatagaat tacagaaact cttcgatgtg aacccttctt ttttagaggt tgtgtactct 240aatgaaagtt tagatgcctg ggaagcagga tgtacatgga tcaccgataa cagagtatcg 300attcaactac gcaaacgttt tcaaaaagct tctttctggt ttggtttttt ttccaaagaa 360gaagtgctgt ctcacgaagc tgttcatgct gtgcgtatga aattttatga accgatcttt 420gaagaggtct tggcatacag cacttctaaa cacttttgga gacgcttttt tggtcccctg 480ttccgatcag cagaagaaac gcatttcttt ctgtttttcg ttttatttgg agcgttttta 540ttcccttggt ttccttggat aggcctttct tgtattcttg ctcctaatat gttctttttt 600tttcgcttat tccgaacaca aatcctattt cgtaaagcaa agaaaaaaat tcgaaaactt 660ttaggtatag aacctctctg ggtcttacta cgcttaacag atagagaaat tcgcctattt 720gctacacagc ccttagctgt gatagaagac ttcgctagga aagaaaagct gaaaagtgtg 780cgctggaggc aaatctatca aagttacttc acctaa81621094211271212PRT213Chlamydia trachomatis40094Leu Arg Ala Ala Leu Asn Thr Leu Glu Phe Leu Ser Ser Pro Pro Ser1 5 10 15Ser Asp Pro Tyr Asp Asp Leu Leu Gln Leu Asn Lys Glu Gly Phe Leu20 25 30
Ala Gly Pro Glu Glu Glu Lys Gln Ala Phe Phe Leu Arg Val Glu Arg35 40 45Thr Leu Ala Glu Ala Pro Val His Pro Thr Pro Phe Pro Ile Glu Leu50 55 60Gln Lys Leu Phe Asp Val Asn Pro Ser Phe Leu Glu Val Val Tyr Ser65 70 75 80Asn Glu Ser Leu Asp Ala Trp Glu Ala Gly Cys Thr Trp Ile Thr Asp85 90 95Asn Arg Val Ser Ile Gln Leu Arg Lys Arg Phe Gln Lys Ala Ser Phe100 105 110Trp Phe Gly Phe Phe Ser Lys Glu Glu Val Leu Ser His Glu Ala Val115 120 125His Ala Val Arg Met Lys Phe Tyr Glu Pro Ile Phe Glu Glu Val Leu130 135 140Ala Tyr Ser Thr Ser Lys His Phe Trp Arg Arg Phe Phe Gly Pro Leu145 150 155 160Phe Arg Ser Ala Glu Glu Thr His Phe Phe Leu Phe Phe Val Leu Phe165 170 175Gly Ala Phe Leu Phe Pro Trp Phe Pro Trp Ile Gly Leu Ser Cys Ile180 185 190Leu Ala Pro Asn Met Phe Phe Phe Phe Arg Leu Phe Arg Thr Gln Ile195 200 205Leu Phe Arg Lys Ala Lys Lys Lys Ile Arg Lys Leu Leu Gly Ile Glu210 215 220Pro Leu Trp Val Leu Leu Arg Leu Thr Asp Arg Glu Ile Arg Leu Phe225 230 235 240Ala Thr Gln Pro Leu Ala Val Ile Glu Asp Phe Ala Arg Lys Glu Lys245 250 255Leu Lys Ser Val Arg Trp Arg Gln Ile Tyr Gln Ser Tyr Phe Thr260 265 27021095211180212DNA
213Chlamydia trachomatis40095atggaccagt tatcacagat acatcaagag ctagctcgtt tagaatttat caatgatcaa 60ctgcagtcgg aaagagcgta catccatgat ttattgtgtg cgataggctt tcctgaaggg 120ttaaagacga tagccgcgat agctaacgaa gtgctttccg aagaagattc ccaaggttaa 1802109621159212PRT213Chlamydia trachomatis40096Met Asp Gln Leu Ser Gln Ile His Gln Glu Leu Ala Arg Leu Glu Phe1 5 10 15Ile Asn Asp Gln Leu Gln Ser Glu Arg Ala Tyr Ile His Asp Leu Leu20 25 30Cys Ala Ile Gly Phe Pro Glu Gly Leu Lys Thr Ile Ala Ala Ile Ala35 40 45Asn Glu Val Leu Ser Glu Glu Asp Ser Gln Gly50 55210972112490212DNA213Chlamydia trachomatis40097atgggtatac gcttagttat tgataaaggg cccttgtctg gaactgttct tattttagaa 60aatgggacga gttggtctct tggcagtgat ggaaaagcta gtgatattct cctgcaagat 120gaaaagcttg ctccctctca gattcgcatc actttaaaag atggcgagta ttatttagaa 180aatttagatg ctttgaggcc ggtttctgtt gatggaacag ttatcactgc ccctgttttg 240ttaaaagatg gggtttcctt tgtaatggga agctgccaag tctcgttttt taaaggggaa 300gaggtagaag gagatataga gttatcgttc cagacagaag gtggtaatga gggagagcct 360gcagcgcaag gctcttcaag cgtttcgtcc gaagctccta aaaaggagac agggaatcca 420agtcttcctt cggaggcaaa ggcttctgga gaagtatcta gttcagcaat agcgaaagaa 480caagagttag cggcgtcctt tttagcttct gttgagaagg agcctggaac accaaaagaa 540gtctctgagc caaaggtctc ttcacaagaa ggacagactc cttctgttac aggagaaaaa 600aaggatcttg agcttccttt ggcaagtcaa gaacaaccta aacaaactac tccatcaggc 660agtggtgaac caacccaatc tcaaaacgcg agtatggaag aaaacagaac gtcgcccgat 720caaaatcagc agccacagct ttcttctgct tcagaatcgg gttctcaaag tcccgaaaat 780
caggagcaac aaccttctca aacgcctccc ccatccccgg aaactccaga gccgtcagga 840gaacctaata gcgctacgga agaaaactcg ccatctccaa tggagaaagc ttccgtaaca 900gaagaaggca gctcagggac gagtgaagaa gaaaaagagg gtgaagaaga tactgctgaa 960agcgcagcaa atgaagagcc aaaggcagag gcttctcaag aagaagagaa gaaagaggaa 1020gataaaggag aggttcttgc tccctttaat gttcaggatc ttttccgttt tgatcaagga 1080atcttccctg ctgagataga agatcttgca cagaaacaag ttgcggttga tttgacgcaa 1140ccatcacgat ttttgttgaa ggttcttgct ggtgcgaata tcggtgctga attccatttg 1200gatagtggga aaacctatat cgtaggaagt gatccgcagg ttgcagacat tgtcttaagt 1260gatatgagta tttcgcgcca acatgcgaag atcattatcg gaaatgataa ttcagttttg 1320attgaagatc tgggtagtaa gaatggcgtg attgttgaag ggcgcaagat tgaacatcaa 1380tctacgctct ctgcgaatca agttgttgct ctaggaacaa cgttattctt acttgtcgac 1440tatgctgctc cttccgatac ggtaatggcg acgatttctt ctgaagatta tgggttattt 1500ggtcgtccgc aatctcctga agagattgct gccagagctg cggaagagga agaagagaag 1560agaaaacgtg ctacgttgcc aacaggtgct tttatattaa ccttgttcat tggagggtta 1620gctctgctct ttggaatagg aacagcttct ttgttccata cgaaggaagt agtttctata 1680gatcaaatcg atttgattca tgatattgaa catgtaattc agcagtttcc aactgtacgg 1740tttacgttca ataagaacaa cggacagttg ttcttaattg ggcatgtaag aaatagcatt 1800gataagagcg agttacttta caaagtggat gctctctcgt ttgtcaagtc ggtagatgat 1860aacgtgatcg atgacgaggc agtatggcaa gagatgaata ttctcttgtc taagaatcca 1920gaatttaaag gtatcagcat gcaatctcca gagccgggga tttttgtaat cagcgggtat 1980ctaaagacag aagaacaagc agcttgtttg gctgattatc taaatctaca ttttaattac 2040ctttcactat tggataataa ggtgattatc gaatcacaag tcatgaaagc tcttgctgga 2100catcttgtgc aatcaggttt tgcgaacgtt catgtgtcct tcaccaatgg tgaagctgtt 2160ttgacaggat atatcaataa taaagatgca gataaattcc gaacggttgt gcaagaactg 2220caagatattg cagggattcg tgcggtgaag aattttgtcg ttttgctgcc tgcagaagaa 2280ggtgttattg atctaaatat gcggtatcca ggccgttatc gggtaaccgg tttttcaaag 2340tgcggggata ttagtattaa tgttgtagtt aatgggcgta ttttaactcg aggcgatatt 2400ttagatggaa tgacggtaac aagcattcaa tcgcattgta tctttttaga acgggaaggg 2460ttgaaatata aaattgagta caataaatag 249021098211829212PRT213Chlamydia trachomatis40098
Met Gly Ile Arg Leu Val Ile Asp Lys Gly Pro Leu Ser Gly Thr Val1 5 10 15Leu Ile Leu Glu Asn Gly Thr Ser Trp Ser Leu Gly Ser Asp Gly Lys20 25 30Ala Ser Asp Ile Leu Leu Gln Asp Glu Lys Leu Ala Pro Ser Gln Ile35 40 45Arg Ile Thr Leu Lys Asp Gly Glu Tyr Tyr Leu Glu Asn Leu Asp Ala50 55 60Leu Arg Pro Val Ser Val Asp Gly Thr Val Ile Thr Ala Pro Val Leu65 70 75 80Leu Lys Asp Gly Val Ser Phe Val Met Gly Ser Cys Gln Val Ser Phe85 90 95Phe Lys Gly Glu Glu Val Glu Gly Asp Ile Glu Leu Ser Phe Gln Thr100 105 110Glu Gly Gly Asn Glu Gly Glu Pro Ala Ala Gln Gly Ser Ser Ser Val115 120 125Ser Ser Glu Ala Pro Lys Lys Glu Thr Gly Asn Pro Ser Leu Pro Ser130 135 140Glu Ala Lys Ala Ser Gly Glu Val Ser Ser Ser Ala Ile Ala Lys Glu145 150 155 160Gln Glu Leu Ala Ala Ser Phe Leu Ala Ser Val Glu Lys Glu Pro Gly165 170 175Thr Pro Lys Glu Val Ser Glu Pro Lys Val Ser Ser Gln Glu Gly Gln180 185 190Thr Pro Ser Val Thr Gly Glu Lys Lys Asp Leu Glu Leu Pro Leu Ala195 200 205Ser Gln Glu Gln Pro Lys Gln Thr Thr Pro Ser Gly Ser Gly Glu Pro210 215 220Thr Gln Ser Gln Asn Ala Ser Met Glu Glu Asn Arg Thr Ser Pro Asp225 230 235 240Gln Asn Gln Gln Pro Gln Leu Ser Ser Ala Ser Glu Ser Gly Ser Gln245 250 255
Ser Pro Glu Asn Gln Glu Gln Gln Pro Ser Gln Thr Pro Pro Pro Ser260 265 270Pro Glu Thr Pro Glu Pro Ser Gly Glu Pro Asn Ser Ala Thr Glu Glu275 280 285Asn Ser Pro Ser Pro Met Glu Lys Ala Ser Val Thr Glu Glu Gly Ser290 295 300Ser Gly Thr Ser Glu Glu Glu Lys Glu Gly Glu Glu Asp Thr Ala Glu305 310 315 320Ser Ala Ala Asn Glu Glu Pro Lys Ala Glu Ala Ser Gln Glu Glu Glu325 330 335Lys Lys Glu Glu Asp Lys Gly Glu Val Leu Ala Pro Phe Asn Val Gln340 345 350Asp Leu Phe Arg Phe Asp Gln Gly Ile Phe Pro Ala Glu Ile Glu Asp355 360 365Leu Ala Gln Lys Gln Val Ala Val Asp Leu Thr Gln Pro Ser Arg Phe370 375 380Leu Leu Lys Val Leu Ala Gly Ala Asn Ile Gly Ala Glu Phe His Leu385 390 395 400Asp Ser Gly Lys Thr Tyr Ile Val Gly Ser Asp Pro Gln Val Ala Asp405 410 415Ile Val Leu Ser Asp Met Ser Ile Ser Arg Gln His Ala Lys Ile Ile420 425 430Ile Gly Asn Asp Asn Ser Val Leu Ile Glu Asp Leu Gly Ser Lys Asn435 440 445Gly Val Ile Val Glu Gly Arg Lys Ile Glu His Gln Ser Thr Leu Ser450 455 460Ala Asn Gln Val Val Ala Leu Gly Thr Thr Leu Phe Leu Leu Val Asp465 470 475 480Tyr Ala Ala Pro Ser Asp Thr Val Met Ala Thr Ile Ser Ser Glu Asp485 490 495Tyr Gly Leu Phe Gly Arg Pro Gln Ser Pro Glu Glu Ile Ala Ala Arg500 505 510Ala Ala Glu Glu Glu Glu Glu Lys Arg Lys Arg Ala Thr Leu Pro Thr
515 520 525Gly Ala Phe Ile Leu Thr Leu Phe Ile Gly Gly Leu Ala Leu Leu Phe530 535 540Gly Ile Gly Thr Ala Ser Leu Phe His Thr Lys Glu Val Val Ser Ile545 550 555 560Asp Gln Ile Asp Leu Ile His Asp Ile Glu His Val Ile Gln Gln Phe565 570 575Pro Thr Val Arg Phe Thr Phe Asn Lys Asn Asn Gly Gln Leu Phe Leu580 585 590Ile Gly His Val Arg Asn Ser Ile Asp Lys Ser Glu Leu Leu Tyr Lys595 600 605Val Asp Ala Leu Ser Phe Val Lys Ser Val Asp Asp Asn Val Ile Asp610 615 620Asp Glu Ala Val Trp Gln Glu Met Asn Ile Leu Leu Ser Lys Asn Pro625 630 635 640Glu Phe Lys Gly Ile Ser Met Gln Ser Pro Glu Pro Gly Ile Phe Val645 650 655Ile Ser Gly Tyr Leu Lys Thr Glu Glu Gln Ala Ala Cys Leu Ala Asp660 665 670Tyr Leu Asn Leu His Phe Asn Tyr Leu Ser Leu Leu Asp Asn Lys Val675 680 685Ile Ile Glu Ser Gln Val Met Lys Ala Leu Ala Gly His Leu Val Gln690 695 700Ser Gly Phe Ala Asn Val His Val Ser Phe Thr Asn Gly Glu Ala Val705 710 715 720Leu Thr Gly Tyr Ile Asn Asn Lys Asp Ala Asp Lys Phe Arg Thr Val725 730 735Val Gln Glu Leu Gln Asp Ile Ala Gly Ile Arg Ala Val Lys Asn Phe740 745 750Val Val Leu Leu Pro Ala Glu Glu Gly Val Ile Asp Leu Asn Met Arg755 760 765Tyr Pro Gly Arg Tyr Arg Val Thr Gly Phe Ser Lys Cys Gly Asp Ile770 775 780
Ser Ile Asn Val Val Val Asn Gly Arg Ile Leu Thr Arg Gly Asp Ile785 790 795 800Leu Asp Gly Met Thr Val Thr Ser Ile Gln Ser His Cys Ile Phe Leu805 810 815Glu Arg Glu Gly Leu Lys Tyr Lys Ile Glu Tyr Asn Lys820 82521099211525212DNA213Chlamydia trachomatis40099ttggaggatt acgtggcttc tcctcatctt cgttccctag cgtgtttaga taacccacaa 60ctacccatag aaacacctct ctttgagcaa gaagctctct cccatgagct tctttctctt 120attcaggtgt tccgtaaatt atctgtccat cttctctctg aaatcgaaaa attatctcag 180aaactaaaac ctgagcttct tgaacttgct gtcctcgtct gtgaaaaatt tctgtacaga 240aagcttgcct gtacagaaga acttgctctc ctaatctccg cagctctgca acatcattta 300gctacttatg ccgtctctcc cataaaaata ggtttacatc ctgaagatct ttcaaaccta 360tctaaatggt taatccttca cgatgttccc ttactcaaaa atatcgaatt cattgcagat 420cctttatgca agaaagctag ctataaaata gaactccctt caggaattct gagacaagac 480atcggggaag agctgtccca tctactgagt gtactcactc cataa 525210100211174212PRT213Chlamydia trachomatis400100Leu Glu Asp Tyr Val Ala Ser Pro His Leu Arg Ser Leu Ala Cys Leu1 5 10 15Asp Asn Pro Gln Leu Pro Ile Glu Thr Pro Leu Phe Glu Gln Glu Ala20 25 30Leu Ser His Glu Leu Leu Ser Leu Ile Gln Val Phe Arg Lys Leu Ser35 40 45Val His Leu Leu Ser Glu Ile Glu Lys Leu Ser Gln Lys Leu Lys Pro50 55 60Glu Leu Leu Glu Leu Ala Val Leu Val Cys Glu Lys Phe Leu Tyr Arg65 70 75 80
Lys Leu Ala Cys Thr Glu Glu Leu Ala Leu Leu Ile Ser Ala Ala Leu85 90 95Gln His His Leu Ala Thr Tyr Ala Val Ser Pro Ile Lys Ile Gly Leu100 105 110His Pro Glu Asp Leu Ser Asn Leu Ser Lys Trp Leu Ile Leu His Asp115 120 125Val Pro Leu Leu Lys Asn Ile Glu Phe Ile Ala Asp Pro Leu Cys Lys130 135 140Lys Ala Ser Tyr Lys Ile Glu Leu Pro Ser Gly Ile Leu Arg Gln Asp145 150 155 160Ile Gly Glu Glu Leu Ser His Leu Leu Ser Val Leu Thr Pro165 170210101211420212DNA213Chlamydia trachomatis400101atggaagatt ttgcagagta catcgttaaa aatttagtta ccgatcccaa cgccgttgag 60attcggtcat ccgaggacaa agccagcgca acccttaagc tggagatcca tgccgcttct 120gaagatattg gaaagatcat cgggagaaaa ggacaaacca tacaagcgct aagaaccatt 180ctaaaacgtg taggcgctag attgcagaaa aaaatccttg ttgagcttgc tcaacctgaa 240aacggctctc tcacagacga agaagttttg tctttagatt atatctctgc agcttctgcc 300gaagatttcg aagaagattc ttcttttgct gaaaatagca tcgaagaaga accttcggtc 360attgtacgat ctcttgcagg tgtatgccca ggttgtagct gctctcatca tcatgattag 420210102211139212PRT213Chlamydia trachomatis400102Met Glu Asp Phe Ala Glu Tyr Ile Val Lys Asn Leu Val Thr Asp Pro1 5 10 15Asn Ala Val Glu Ile Arg Ser Ser Glu Asp Lys Ala Ser Ala Thr Leu20 25 30Lys Leu Glu Ile His Ala Ala Ser Glu Asp Ile Gly Lys Ile Ile Gly35 40 45
Arg Lys Gly Gln Thr Ile Gln Ala Leu Arg Thr Ile Leu Lys Arg Val50 55 60Gly Ala Arg Leu Gln Lys Lys Ile Leu Val Glu Leu Ala Gln Pro Glu65 70 75 80Asn Gly Ser Leu Thr Asp Glu Glu Val Leu Ser Leu Asp Tyr Ile Ser85 90 95Ala Ala Ser Ala Glu Asp Phe Glu Glu Asp Ser Ser Phe Ala Glu Asn100 105 110Ser Ile Glu Glu Glu Pro Ser Val Ile Val Arg Ser Leu Ala Gly Val115 120 125Cys Pro Gly Cys Ser Cys Ser His His His Asp130 135210103211279212DNA213Chlamydia trachomatis400103atgaaggaag aaattctcgc gctacttgat catttatata cggagcagga aagacgatta 60atgtcgctag ggacgacgat tgttcctgga ttgacgaaag aggatctttt acagcctatg 120gattatgatg aacttgagga gaacccttct tttagatttg aagaaggagt tttgaatgga 180ataggagaga ctcgagccgc attatattct tttttttctg atctagaaga ctccttttgc 240gtggagtctt ctagcgatac gagcctctgt aaggattag 27921010421192212PRT213Chlamydia trachomatis400104Met Lys Glu Glu Ile Leu Ala Leu Leu Asp His Leu Tyr Thr Glu Gln1 5 10 15Glu Arg Arg Leu Met Ser Leu Gly Thr Thr Ile Val Pro Gly Leu Thr20 25 30Lys Glu Asp Leu Leu Gln Pro Met Asp Tyr Asp Glu Leu Glu Glu Asn35 40 45Pro Ser Phe Arg Phe Glu Glu Gly Val Leu Asn Gly Ile Gly Glu Thr50 55 60Arg Ala Ala Leu Tyr Ser Phe Phe Ser Asp Leu Glu Asp Ser Phe Cys
65 70 75 80Val Glu Ser Ser Ser Asp Thr Ser Leu Cys Lys Asp85 90210105211507212DNA213Chlamydia trachomatis400105atgtggcata aagaaccaat gcatgctgta ttacagctac ctgaaacacc tctggttaca 60ggaacaacga actctgcaac ggctgatgag attataacac gatttgctaa ggattctaac 120cctctcatcg ttactgttta ctatatttac cagtctgttc tcgtcgcgca aaataacttg 180tctctagttg cagagcaatt gcaagctaat gctgccgcgc aaacattcct gaacaatgaa 240gaagcgctat accaatacac taccattcca aaaaaccaag taaactctca aaactcctct 300tatttacaaa acgtacaatc ggttaaccaa gcagtcggag catctagaca agcgattcaa 360aaccaaatat ctggtcttgg aaatgcttct caagtgattt ccagtaactt gaacacgaat 420aataatatta ttcaacagtc cctacaagtc ggtcaggcgc tcattcaaac gttttcacaa 480atcgttagct tgatcgcgaa tatctaa 507210106211168212PRT213Chlamydia trachomatis400106Met Trp His Lys Glu Pro Met His Ala Val Leu Gln Leu Pro Glu Thr1 5 10 15Pro Leu Val Thr Gly Thr Thr Asn Ser Ala Thr Ala Asp Glu Ile Ile20 25 30Thr Arg Phe Ala Lys Asp Ser Asn Pro Leu Ile Val Thr Val Tyr Tyr35 40 45Ile Tyr Gln Ser Val Leu Val Ala Gln Asn Asn Leu Ser Leu Val Ala50 55 60Glu Gln Leu Gln Ala Asn Ala Ala Ala Gln Thr Phe Leu Asn Asn Glu65 70 75 80Glu Ala Leu Tyr Gln Tyr Thr Thr Ile Pro Lys Asn Gln Val Asn Ser85 90 95Gln Asn Ser Ser Tyr Leu Gln Asn Val Gln Ser Val Asn Gln Ala Val100 105 110
Gly Ala Ser Arg Gln Ala Ile Gln Asn Gln Ile Ser Gly Leu Gly Asn115 120 125Ala Ser Gln Val Ile Ser Ser Asn Leu Asn Thr Asn Asn Asn Ile Ile130 135 140Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile Gln Thr Phe Ser Gln145 150 155 160Ile Val Ser Leu Ile Ala Asn Ile165210107211486212DNA213Chlamydia psittaci400107gtgcgcatac taccctttga tccttatgga gcattacctc ctcaaggagt aaataaagat 60cctcatagca atataccttt aaatcagaag atttctgatg aaatagctaa aaatgaagct 120atgcgtttgg ctttgcttgc tattactgat caagaaaaag acaaaagaaa gagaaaacat 180cggtttaaaa ttcttaaccg caaacaagca aaagtgctcc tatctcaatt gcgaaatgta 240gatttagact ttcaaagttt gaaaaatgct ggtttaaaag aagaagaaga taacgaagaa 300gataatgaag aatcttcaaa acaagggaaa actttccata ttgctagtag taagaagcct 360gtgaagatag gggcatctgc agctcaggct attgctgatg ctgcagaggc ttgggttatt 420gctcgcaata gaggagtctt ggatatggcc tccctattat tctggcataa agatgatgag 480tgttaa 486210108211161212PRT213Chlamydia psittaci400108Val Arg Ile Leu Pro Phe Asp Pro Tyr Gly Ala Leu Pro Pro Gln Gly1 5 10 15Val Asn Lys Asp Pro His Ser Asn Ile Pro Leu Asn Gln Lys Ile Ser20 25 30Asp Glu Ile Ala Lys Asn Glu Ala Met Arg Leu Ala Leu Leu Ala Ile35 40 45Thr Asp Gln Glu Lys Asp Lys Arg Lys Arg Lys His Arg Phe Lys Ile50 55 60
Leu Asn Arg Lys Gln Ala Lys Val Leu Leu Ser Gln Leu Arg Asn Val65 70 75 80Asp Leu Asp Phe Gln Ser Leu Lys Asn Ala Gly Leu Lys Glu Glu Glu85 90 95Asp Asn Glu Glu Asp Asn Glu Glu Ser Ser Lys Gln Gly Lys Thr Phe100 105 110His Ile Ala Ser Ser Lys Lys Pro Val Lys Ile Gly Ala Ser Ala Ala115 120 125Gln Ala Ile Ala Asp Ala Ala Glu Ala Trp Val Ile Ala Arg Asn Arg130 135 140Gly Val Leu Asp Met Ala Ser Leu Leu Phe Trp His Lys Asp Asp Glu145 150 155 160Cys210109211447212DNA213Chlamydia psittaci400109atgaaaagtg aacgtctgaa aaaactcgaa tctgaattac gtgacttaac ccagtggatg 60caattgggtt tagttccaaa aaaagaaatc gatagacata aggaagaaat acgttcttta 120gaaaacaaaa tccatgaaga gaaggaacgc ctgcaacttc taaaagaaag tggcgaagtt 180gaagagtttg ttactccaag acgtagccct gcaaaaacgg tatatcctga cggtccaagc 240atgtcagata tggaatttgt tgaagctaca gagacagaaa ttgatattga tccaggtgaa 300accgtagagc tcgaacttcc tgatgaagaa cgtgaagaag gcgctgtaga aatcgattat 360tccagtgatg acgatgaaga tcctttcagt gatcgcaatc gttggagacg tgggggcatt 420gttgaccccg atgctaatga atggtaa 447210110211148212PRT213Chlamydia psittaci400110Met Lys Ser Glu Arg Leu Lys Lys Leu Glu Ser Glu Leu Arg Asp Leu1 5 10 15Thr Gln Trp Met Gln Leu Gly Leu Val Pro Lys Lys Glu Ile Asp Arg20 25 30
His Lys Glu Glu Ile Arg Ser Leu Glu Asn Lys Ile His Glu Glu Lys35 40 45Glu Arg Leu Gln Leu Leu Lys Glu Ser Gly Glu Val Glu Glu Phe Val50 55 60Thr Pro Arg Arg Ser Pro Ala Lys Thr Val Tyr Pro Asp Gly Pro Ser65 70 75 80Met Ser Asp Met Glu Phe Val Glu Ala Thr Glu Thr Glu Ile Asp Ile85 90 95Asp Pro Gly Glu Thr Val Glu Leu Glu Leu Pro Asp Glu Glu Arg Glu100 105 110Glu Gly Ala Val Glu Ile Asp Tyr Ser Ser Asp Asp Asp Glu Asp Pro115 120 125Phe Ser Asp Arg Asn Arg Trp Arg Arg Gly Gly Ile Val Asp Pro Asp130 135 140Ala Asn Glu Trp145210111211972212DNA213Chlamydia psittaci400111atgaaacggc tgcttttttg cgtttgcgca ctctctttct catgctttac ctacggttca 60gctctaaaac aagattcttc tgttatgaag gaaaccttcc gtaacaacta cggaattatt 120gtatcaggaa aagattgggt aaaacgaggc tgtgatggga caatcaccaa agttttaaag 180gatgggtcta ctctttatga aatttacgtt cagggtcttc ttcatggcga gataacgtta 240acattccccc attctacgac tcttgctgta ataaaaactt acgatcaggg aaggcttgtt 300tcatataaaa cattcttttc taatggttta ccttctcaag aagaattata ccaagaagat 360ggctctcttg ttgtgactcg ctggcctgac aacaaaaata gcgataccat caccgatcct 420tattttactg agactaccta ccagggtcgt gtacttgaag ggagctactc ctcatttaat 480gggaaatata catcaacaat tcgcaatgga gaaggcatac gctcgaactt ttctccaagt 540aacgtccttc tttctgaaga aacctttaac gatggcgtta tggtaaaaag aactaccttc 600tatgctacta gagatcctga aactataacg cattatatta atggccaacc tcatggattg 660cgtttaacct atctccctgg agggattcct aacactattg aagaatggcg ttatggttac 720caagatggaa ctacaacagt atttaaaaac ggttgcaaag ctgctgagat tccttttgta 780
aaaggatcta aggaaggatg tgaattacgc tataatgaag acgaagttat tgccgaagaa 840gtgtcttgga gaaataactt ccctcatggt atgagaaaga tctatgctgc cggtgtttat 900aaatgcgagt ggtaccaccg cggacgccta gtctcaaaag ctaagttcga gagactcaat 960aatgcaggat aa 972210112211323212PRT213Chlamydia psittaci400112Met Lys Arg Leu Leu Phe Cys Val Cys Ala Leu Ser Phe Ser Cys Phe1 5 10 15Thr Tyr Gly Ser Ala Leu Lys Gln Asp Ser Ser Val Met Lys Glu Thr20 25 30Phe Arg Asn Asn Tyr Gly Ile Ile Val Ser Gly Lys Asp Trp Val Lys35 40 45Arg Gly Cys Asp Gly Thr Ile Thr Lys Val Leu Lys Asp Gly Ser Thr50 55 60Leu Tyr Glu Ile Tyr Val Gln Gly Leu Leu His Gly Glu Ile Thr Leu65 70 75 80Thr Phe Pro His Ser Thr Thr Leu Ala Val Ile Lys Thr Tyr Asp Gln85 90 95Gly Arg Leu Val Ser Tyr Lys Thr Phe Phe Ser Asn Gly Leu Pro Ser100 105 110Gln Glu Glu Leu Tyr Gln Glu Asp Gly Ser Leu Val Val Thr Arg Trp115 120 125Pro Asp Asn Lys Asn Ser Asp Thr Ile Thr Asp Pro Tyr Phe Thr Glu130 135 140Thr Thr Tyr Gln Gly Arg Val Leu Glu Gly Ser Tyr Ser Ser Phe Asn145 150 155 160Gly Lys Tyr Thr Ser Thr Ile Arg Asn Gly Glu Gly Ile Arg Ser Asn165 170 175Phe Ser Pro Ser Asn Val Leu Leu Ser Glu Glu Thr Phe Asn Asp Gly180 185 190
Val Met Val Lys Arg Thr Thr Phe Tyr Ala Thr Arg Asp Pro Glu Thr195 200 205Ile Thr His Tyr Ile Asn Gly Gln Pro His Gly Leu Arg Leu Thr Tyr210 215 220Leu Pro Gly Gly Ile Pro Asn Thr Ile Glu Glu Trp Arg Tyr Gly Tyr225 230 235 240Gln Asp Gly Thr Thr Thr Val Phe Lys Asn Gly Cys Lys Ala Ala Glu245 250 255Ile Pro Phe Val Lys Gly Ser Lys Glu Gly Cys Glu Leu Arg Tyr Asn260 265 270Glu Asp Glu Val Ile Ala Glu Glu Val Ser Trp Arg Asn Asn Phe Pro275 280 285His Gly Met Arg Lys Ile Tyr Ala Ala Gly Val Tyr Lys Cys Glu Trp290 295 300Tyr His Arg Gly Arg Leu Val Ser Lys Ala Lys Phe Glu Arg Leu Asn305 310 315 320Asn Ala Gly210113211975212DNA213Chlamydia psittaci400113atgacatctg caatgcctcg tgtggctagc cttgtagtgg gcagtagaaa tgtgtttatg 60caaacagcta tgcagggagc caagaaaggc gatgtaggct gctgcattag gcagtttgtt 120acgaatggaa acaaccattt agcacgcttt gtcggaagta caaaaaatat agataaggca 180tttaagtttg ctaaatccgt ctctgaattt agctgcggtg ttattgaaag cacaggtgat 240acaggacctg ctcttcagat aagtaaaaac gttgcatcga ctttaagcac ggctagaaat 300gttgtcgcct tgtctaatgt gttcactggt gcgattcctg gattcgtcct ttcctcaaag 360aattgctata atcatattaa gaaatgtttt gctccagaaa ctgagtacga ttgcggtagt 420attgagaaag gcttgcctta taacaagctt tatctcacta agggtgatca tgtgttgggt 480gcgattaaag aaggttgctc tgcagttggc gcgggaactt acgttgcaac gtttggtgtc 540agccgtccgg tgctcttggc aaacaagctt gctcacaagc ctttcctctc ttcaggagtg 600aaggcagcat tttgtaacag cgtaacctat atgatgacag caaaccacgc tgcaggggtt 660atcggcggag cagcagcctt gctctatgaa aaccgtgtat ataaacgcgc ttctgaggga 720
ttgttagctt ctaaaatgac agagagtctg gattctgaag tttacgatca agtgtctaaa 780ggcttgaaag agtcgcacta ccaagctgtt aaaaaaacaa tacttggaat tttagaaaaa 840gcatttgaat tgatcgctga tatattcgat ttcattgctt taccaattac tgcttcagtt 900cgcttggcaa taaaagcggg attagtcaca gtatccagtg gtttcggtct ttacagcgtc 960tgggtcaatt cttaa 975210114211324212PRT213Chlamydia psittaci400114Met Thr Ser Ala Met Pro Arg Val Ala Ser Leu Val Val Gly Ser Arg1 5 10 15Asn Val Phe Met Gln Thr Ala Met Gln Gly Ala Lys Lys Gly Asp Val20 25 30Gly Cys Cys Ile Arg Gln Phe Val Thr Asn Gly Asn Asn His Leu Ala35 40 45Arg Phe Val Gly Ser Thr Lys Asn Ile Asp Lys Ala Phe Lys Phe Ala50 55 60Lys Ser Val Ser Glu Phe Ser Cys Gly Val Ile Glu Ser Thr Gly Asp65 70 75 80Thr Gly Pro Ala Leu Gln Ile Ser Lys Asn Val Ala Ser Thr Leu Ser85 90 95Thr Ala Arg Asn Val Val Ala Leu Ser Asn Val Phe Thr Gly Ala Ile100 105 110Pro Gly Phe Val Leu Ser Ser Lys Asn Cys Tyr Asn His Ile Lys Lys115 120 125Cys Phe Ala Pro Glu Thr Glu Tyr Asp Cys Gly Ser Ile Glu Lys Gly130 135 140Leu Pro Tyr Asn Lys Leu Tyr Leu Thr Lys Gly Asp His Val Leu Gly145 150 155 160Ala Ile Lys Glu Gly Cys Ser Ala Val Gly Ala Gly Thr Tyr Val Ala165 170 175Thr Phe Gly Val Ser Arg Pro Val Leu Leu Ala Asn Lys Leu Ala His180 185 190
Lys Pro Phe Leu Ser Ser Gly Val Lys Ala Ala Phe Cys Asn Ser Val195 200 205Thr Tyr Met Met Thr Ala Asn His Ala Ala Gly Val Ile Gly Gly Ala210 215 220Ala Ala Leu Leu Tyr Glu Asn Arg Val Tyr Lys Arg Ala Ser Glu Gly225 230 235 240Leu Leu Ala Ser Lys Met Thr Glu Ser Leu Asp Ser Glu Val Tyr Asp245 250 255Gln Val Ser Lys Gly Leu Lys Glu Ser His Tyr Gln Ala Val Lys Lys260 265 270Thr Ile Leu Gly Ile Leu Glu Lys Ala Phe Glu Leu Ile Ala Asp Ile275 280 285Phe Asp Phe Ile Ala Leu Pro Ile Thr Ala Ser Val Arg Leu Ala Ile290 295 300Lys Ala Gly Leu Val Thr Val Ser Ser Gly Phe Gly Leu Tyr Ser Val305 310 315 320Trp Val Asn Ser210115211423212DNA213Chlamydia psittaci400115atgagtttgg attttttaga agaattttac cgtcgttcta tttgtaacaa aggaacggca 60tttcctgagg gcttcatgga tattgccgat gtcctatccc attctgcgtc tgaacttaaa 120atcgagtcta tgagcgatct tcctgttaac aatttcatca ttgcagaatc ggcagataaa 180ctcactttat ttaatgcaga ttttgctgtt tggttagtgc ctgagcttgt ccaaggagaa 240gctgtgactc gaggatacat cgctttatac cattctggag gggattatac tccagaaatg 300gcatttcaag cctctgggga gtacaatcaa tcagcattaa ttcttgaagc acttcagata 360tatctacaag acataaaaga taccgaaagc acgctacgct ctttccgctt taatcaagac 420tag423210116211140212PRT213Chlamydia psittaci
400116Met Ser Leu Asp Phe Leu Glu Glu Phe Tyr Arg Arg Ser Ile Cys Asn1 5 10 15Lys Gly Thr Ala Phe Pro Glu Gly Phe Met Asp Ile Ala Asp Val Leu20 25 30Ser His Ser Ala Ser Glu Leu Lys Ile Glu Ser Met Ser Asp Leu Pro35 40 45Val Asn Asn Phe Ile Ile Ala Glu Ser Ala Asp Lys Leu Thr Leu Phe50 55 60Asn Ala Asp Phe Ala Val Trp Leu Val Pro Glu Leu Val Gln Gly Glu65 70 75 80Ala Val Thr Arg Gly Tyr Ile Ala Leu Tyr His Ser Gly Gly Asp Tyr85 90 95Thr Pro Glu Met Ala Phe Gln Ala Ser Gly Glu Tyr Asn Gln Ser Ala100 105 110Leu Ile Leu Glu Ala Leu Gln Ile Tyr Leu Gln Asp Ile Lys Asp Thr115 120 125Glu Ser Thr Leu Arg Ser Phe Arg Phe Asn Gln Asp130 135 140210117211849212DNA213Chlamydia psittaci400117atggaattaa ataaaacatc cgagtctttg tacaattgca agacagatcg ccattcagta 60caacaagaag taggtccaga gcctaaagat aaccgtgacg ttaaagtgtt ttctttagaa 120ggccgtcaac aatcaaaaca agatcgtcag gataaagttt ccagtaaaga ttctcgtcaa 180gaatctcgag gagctgatga taagcatgta gaagagaaaa catcagcagt atcttctaaa 240gaagaagata aagaagagag tgatggtttc atggcttatg acaatcctac agcaggcatg 300gcatttgtag atgttgctgc ttctgtatct agtgaagccg ttgtagaaag tactacagta 360gctatcggca gtgcagattt acagtgggtg caagatgtta ttgctagtac tgtagaatct 420atgatggttg ctgatgttaa cggtcagcaa ttaatcgagt tagttttaga tgctgaaggt 480aatgttcctg acatttttgc aggtgcgaat ttaacattag tacaaacagg aacagaccta 540tctgtaaaat tctctaattt tgtagataat gctcagatgg cagaagcaat gagtctcatt 600
gtgaataacc cttctcagct tgctggttta gtagaggcat taaaaaatcg tcatttgaat 660ttgacagaat tggctgttgg atcaagtatt gtacaattgc caactattga agaggtgcaa 720acacctctac atatgattgc tgctacaatt catcaaagag atgaagagag agatcaagaa 780ggacaagatc agcagcaaca gcaagatcaa gaacaaaacc aatataaagt tgaagaagca 840cgtttgtaa 849210118211282212PRT213Chlamydia psittaci400118Met Glu Leu Asn Lys Thr Ser Glu Ser Leu Tyr Asn Cys Lys Thr Asp1 5 10 15Arg His Ser Val Gln Gln Glu Val Gly Pro Glu Pro Lys Asp Asn Arg20 25 30Asp Val Lys Val Phe Ser Leu Glu Gly Arg Gln Gln Ser Lys Gln Asp35 40 45Arg Gln Asp Lys Val Ser Ser Lys Asp Ser Arg Gln Glu Ser Arg Gly50 55 60Ala Asp Asp Lys His Val Glu Glu Lys Thr Ser Ala Val Ser Ser Lys65 70 75 80Glu Glu Asp Lys Glu Glu Ser Asp Gly Phe Met Ala Tyr Asp Asn Pro85 90 95Thr Ala Gly Met Ala Phe Val Asp Val Ala Ala Ser Val Ser Ser Glu100 105 110Ala Val Val Glu Ser Thr Thr Val Ala Ile Gly Ser Ala Asp Leu Gln115 120 125Trp Val Gln Asp Val Ile Ala Ser Thr Val Glu Ser Met Met Val Ala130 135 140Asp Val Asn Gly Gln Gln Leu Ile Glu Leu Val Leu Asp Ala Glu Gly145 150 155 160Asn Val Pro Asp Ile Phe Ala Gly Ala Asn Leu Thr Leu Val Gln Thr165 170 175Gly Thr Asp Leu Ser Val Lys Phe Ser Asn Phe Val Asp Asn Ala Gln180 185 190
Met Ala Glu Ala Met Ser Leu Ile Val Asn Asn Pro Ser Gln Leu Ala195 200 205Gly Leu Val Glu Ala Leu Lys Asn Arg His Leu Asn Leu Thr Glu Leu210 215 220Ala Val Gly Ser Ser Ile Val Gln Leu Pro Thr Ile Glu Glu Val Gln225 230 235 240Thr Pro Leu His Met Ile Ala Ala Thr Ile His Gln Arg Asp Glu Glu245 250 255Arg Asp Gln Glu Gly Gln Asp Gln Gln Gln Gln Gln Asp Gln Glu Gln260 265 270Asn Gln Tyr Lys Val Glu Glu Ala Arg Leu275 280210119211261212DNA213Chlamydia psittaci400119atgagtagtg gtagcgggag cagttgctca gcatttaatt ttaatgacat gctcaatggc 60gtatgtaagt acgtccaagg tgtgcaacaa tatttaacag aattagaaac ctcaacgcaa 120ggtactgttg acttaggtac gatgtttaat ttgcagtttc gtatgcaaat tttatcacag 180tacatggaag cagtatccaa catcttgaca gcagtgaaca cagagatgat tactatggca 240agagctgtta aaggaagtta a26121012021186212PRT213Chlamydia psittaci400120Met Ser Ser Gly Ser Gly Ser Ser Cys Ser Ala Phe Asn Phe Asn Asp1 5 10 15Met Leu Asn Gly Val Cys Lys Tyr Val Gln Gly Val Gln Gln Tyr Leu20 25 30Thr Glu Leu Glu Thr Ser Thr Gln Gly Thr Val Asp Leu Gly Thr Met35 40 45Phe Asn Leu Gln Phe Arg Met Gln Ile Leu Ser Gln Tyr Met Glu Ala50 55 60
Val Ser Asn Ile Leu Thr Ala Val Asn Tnr Glu Met Ile Thr Met Ala65 70 75 80Arg Ala Val Lys Gly Ser85210121211729212DNA213Chlamydia psittaci400121atggaacctt atttagattt attagataaa aacatcaaag aaaaacatat gttagatcat 60cccttttata tgaagtggtc taagggagaa ttaacgaaag agcagttaaa agaatacgct 120aaggattact acctccacat caaagctttt cctcgttatc tttcagcagt tcatagccgc 180tgtgataatt tagaagcgcg taaattgctt cttgataacc ttatggatga agaaacaggc 240catcctaatc acatagatct atggaaaaat tttgcctatg cgttaggtgt tactgaggaa 300gaattagaaa atcatgttcc tagtgcagca gcacaaaaga aagtggatac atttttacgt 360tggtgtactg gagattcctt atctgctggt gtagctgctt tatataccta tgaaagccaa 420atccctacag ttgcagagac taaaatctcg ggattaaaac agtatttcgg ctttacggct 480cctgaagatt atgagtactt cacagtacat caagatgttg atgtaagaca ttctcgtgaa 540gaaaaagaat taatagagtc gttgctaaat aatgatagcg ataaggttct acaagcttca 600aaagaagttt gtgatgcttt atacggcttt ttagatactt tcttagatga aaaagacgcc 660tgctcagcaa cttcatcttc tgttgcggat tctaaaccat cttcatgttg ttgtcgttgc 720cgtcattaa 729210122211242212PRT213Chlamydia psittaci400122Met Glu Pro Tyr Leu Asp Leu Leu Asp Lys Asn Ile Lys Glu Lys His1 5 10 15Met Leu Asp His Pro Phe Tyr Met Lys Trp Ser Lys Gly Glu Leu Thr20 25 30Lys Glu Gln Leu Lys Glu Tyr Ala Lys Asp Tyr Tyr Leu His Ile Lys35 40 45Ala Phe Pro Arg Tyr Leu Ser Ala Val His Ser Arg Cys Asp Asn Leu50 55 60Glu Ala Arg Lys Leu Leu Leu Asp Asn Leu Met Asp Glu Glu Thr Gly
65 70 75 80His Pro Asn His Ile Asp Leu Trp Lys Asn Phe Ala Tyr Ala Leu Gly85 90 95Val Thr Glu Glu Glu Leu Glu Asn His Val Pro Ser Ala Ala Ala Gln100 105 110Lys Lys Val Asp Thr Phe Leu Arg Trp Cys Thr Gly Asp Ser Leu Ser115 120 125Ala Gly Val Ala Ala Leu Tyr Thr Tyr Glu Ser Gln Ile Pro Thr Val130 135 140Ala Glu Thr Lys Ile Ser Gly Leu Lys Gln Tyr Phe Gly Phe Thr Ala145 150 155 160Pro Glu Asp Tyr Glu Tyr Phe Thr Val His Gln Asp Val Asp Val Arg165 170 175His Ser Arg Glu Glu Lys Glu Leu Ile Glu Ser Leu Leu Asn Asn Asp180 185 190Ser Asp Lys Val Leu Gln Ala Ser Lys Glu Val Cys Asp Ala Leu Tyr195 200 205Gly Phe Leu Asp Thr Phe Leu Asp Glu Lys Asp Ala Cys Ser Ala Thr210 215 220Ser Ser Ser Val Ala Asp Ser Lys Pro Ser Ser Cys Cys Cys Arg Cys225 230 235 240Arg His210123211234212DNA213Chlamydia psittaci400123atgaatgaag gtattcaaac cgtttctttt aacaagacac accgactcac cgcgaaatct 60acagttagtt tagaaatgcc cgtagcgaca cagaaacttc aaggtaaaga aggcatgccc 120gcagcggcaa gtttagaagc tgatttctta agagcagaag ctatacttgc agaaatgcgt 180gaaattcgcg gctgtttaga acattcattg gaaacgctag ttcctagaga ctag23421012421177212PRT
213Chlamydia psittaci400124Met Asn Glu Gly Ile Gln Thr Val Ser Phe Asn Lys Thr His Arg Leu1 5 10 15Thr Ala Lys Ser Thr Val Ser Leu Glu Met Pro Val Ala Thr Gln Lys20 25 30Leu Gln Gly Lys Glu Gly Met Pro Ala Ala Ala Ser Leu Glu Ala Asp35 40 45Phe Leu Arg Ala Glu Ala Ile Leu Ala Glu Met Arg Glu Ile Arg Gly50 55 60Cys Leu Glu His Ser Leu Glu Thr Leu Val Pro Arg Asp65 70 75210125211231212DNA213Chlamydia psittaci400125atgaaaaata aactacatga tttgttgaat cagctttatg aaaatcaaaa atctcgttta 60caaagcatgg gggagcagat cgtcccgaat ttaacttctg atgatgtatt acagcccatg 120gatttttcta tgctggaaga aaatcctttc tttcggtttg aggaaggtgt tctttctggt 180cttggagagg ctcgggcggc gattttggca ttatttgccg aagaaggtta a 23121012621176212PRT213Chlamydia psittaci400126Met Lys Asn Lys Leu His Asp Leu Leu Asn Gln Leu Tyr Glu Asn Gln1 5 10 15Lys Ser Arg Leu Gln Ser Met Gly Glu Gln Ile Val Pro Asn Leu Thr20 25 30Ser Asp Asp Val Leu Gln Pro Met Asp Phe Ser Met Leu Glu Glu Asn35 40 45Pro Phe Phe Arg Phe Glu Glu Gly Phe Phe Leu Val Leu Glu Arg Leu50 55 60Gly Arg Arg Phe Trp His Tyr Leu Pro Lys Lys Val65 70 75
210127211519212DNA213Chlamydia psittaci400127atgtggttct cttctccagc acttcaaact agccctagag ccgctattga cgttcccggg 60acatctatca caggtggacc aaatacagca acagccgatg aaattattgc aaaatttgcg 120aaagattcca atcctctgat tatcaccgtg tactatgtat accagtctgt attggtagcg 180caggacaact tatctattat cgctcaagaa cttcagtcta atgcttctgc ccagactttt 240ttaaacaacc aagaagcttt ataccaatac gtaaccatac ctaagaacaa attaaacgat 300aactcgtcgg cgttcttaca agatgttcaa tcaacaaacc aagctgttgg agcttctcga 360caagcactac aaaaccaaat ttcaggttta ggaaatggtg ctcaggttat ctcaagtaac 420ttgaacacga acaataacat tatccaacag tctctacaag taggccaggc gttaattcaa 480acgttctcac aaattgtaag tttgatagca aacatttaa 519210128211172212PRT213Chlamydia psittaci400128Met Trp Phe Ser Ser Pro Ala Leu Gln Thr Ser Pro Arg Ala Ala Ile1 5 10 15Asp Val Pro Gly Thr Ser Ile Thr Gly Gly Pro Asn Thr Ala Thr Ala20 25 30Asp Glu Ile Ile Ala Lys Phe Ala Lys Asp Ser Asn Pro Leu Ile Ile35 40 45Thr Val Tyr Tyr Val Tyr Gln Ser Val Leu Val Ala Gln Asp Asn Leu50 55 60Ser Ile Ile Ala Gln Glu Leu Gln Ser Asn Ala Ser Ala Gln Thr Phe65 70 75 80Leu Asn Asn Gln Glu Ala Leu Tyr Gln Tyr Val Thr Ile Pro Lys Asn85 90 95Lys Leu Asn Asp Asn Ser Ser Ala Phe Leu Gln Asp Val Gln Ser Thr100 105 110Asn Gln Ala Val Gly Ala Ser Arg Gln Ala Leu Gln Asn Gln Ile Ser115 120 125
Gly Leu Gly Asn Gly Ala Gln Val Ile Ser Ser Asn Leu Asn Thr Asn130 135 140Asn Asn Ile Ile Gln Gln Ser Leu Gln Val Gly Gln Ala Leu Ile Gln145 150 155 160Thr Phe Ser Gln Ile Val Ser Leu Ile Ala Asn Ile165 1702101292111443212DNA213Chlamydia psittaci400129atgaacactc ccctgccttc agcagttccc tctaccaaca tgacgttaaa ggaagatgcc 60tcctcttcat cctcagcatc aacatcttcc agtattttaa agactgcagc aggagatgtt 120gcgctttccg tattcacttc cgaaggaacc acaccagctt ccttaaactc tctagttgct 180cttgcccttg cgcaaatttc tgcagcttct ggagaaaatg ccaatccttt acaagattgt 240gctcataatc ttgtcttcct ttctccagaa actattgaag ttgaaatact catctctgat 300cttcttcaaa ctttagagac tacagatctt ataaacacac aggaagagtc ctcaagttta 360ggaaaacaag aacagcgcct tcctcaagaa ggatgcaaac cacaggattt agcaccaaga 420tctacaatag attcgtacgg cacaccgaaa gcattacaac aacctgccgt gaaactcgct 480gtccgctatt cttctgcaaa ggctcctgat tctcggcctt atacaagttc ctcctcaccg 540cagcatactt cgggacaatt tgcccaacgt gctgcccagg cgccaggaat actgcaacac 600tcccaaacaa aaaaagatgg agagcaaatt tcttctcaat ctcacaattc ctttattgcg 660gagaaaaaag aacagcagat tttcaccaca aagtctcaag aatctcagca ggatcgtgaa 720aaccgagatc aaaaacaaga tcgccaacat gatggccaac atcaagatga tgatgatgat 780cagcaaaaag gtagggggaa aaaatataaa tcaaagactt ctgccgaagc agttcctgct 840gatctctccg tagcgcatct acgctatctt aatgaggtac gccaatctcg agaaattcat 900gttgaggaag aaaaaacatt taagaagaaa gcgcaatctc cgatggcact tttttctgct 960ccaactcctc cagcaggatt taccccaatt cctactccaa agattgaaaa cgtattcata 1020cgttttatga aacttatgga aaggattctg ggacaggccg aagccgaagc ccaagaatta 1080tatcttcgcg ttaaagagcg taccgataat gtagacacat taatgctgct catttctaaa 1140attaactctg aaaaaggtgc tattgactgg cgagatgatt cagaaatgaa agctctcgtg 1200gatcaagcaa aaaaactggg tgtaacgata acagatactc tgcaatggtc tgaagaagag 1260aaaaaacttc taaaagaaaa tattcagatg cgtaaagaaa acctggaaaa aatcacccag 1320cttgaacgaa cagacatgca aaggcatctt caagaagtct cacaatgtca ccaagcacgc 1380tccaacgcct tgaaacttct caaagaactc atggatacct ttatttacaa tttgcgtccg 1440
taa1443210130211480212PRT213Chlamydia psittaci400130Met Asn Thr Pro Leu Pro Ser Ala Val Pro Ser Thr Asn Met Thr Leu1 5 10 15Lys Glu Asp Ala Ser Ser Ser Ser Ser Ala Ser Thr Ser Ser Ser Ile20 25 30Leu Lys Thr Ala Ala Gly Asp Val Ala Leu Ser Val Phe Thr Ser Glu35 40 45Gly Thr Thr Pro Ala Ser Leu Asn Ser Leu Val Ala Leu Ala Leu Ala50 55 60Gln Ile Ser Ala Ala Ser Gly Glu Asn Ala Asn Pro Leu Gln Asp Cys65 70 75 80Ala His Asn Leu Val Phe Leu Ser Pro Glu Thr Ile Glu Val Glu Ile85 90 95Leu Ile Ser Asp Leu Leu Gln Thr Leu Glu Thr Thr Asp Leu Ile Asn100 105 110Thr Gln Glu Glu Ser Ser Ser Leu Gly Lys Gln Glu Gln Arg Leu Pro115 120 125Gln Glu Gly Cys Lys Pro Gln Asp Leu Ala Pro Arg Ser Thr Ile Asp130 135 140Ser Tyr Gly Thr Pro Lys Ala Leu Gln Gln Pro Ala Val Lys Leu Ala145 150 155 160Val Arg Tyr Ser Ser Ala Lys Ala Pro Asp Ser Arg Pro Tyr Thr Ser165 170 175Ser Ser Ser Pro Gln His Thr Ser Gly Gln Phe Ala Gln Arg Ala Ala180 185 190Gln Ala Pro Gly Ile Leu Gln His Ser Gln Thr Lys Lys Asp Gly Glu195 200 205Gln Ile Ser Ser Gln Ser His Asn Ser Phe Ile Ala Glu Lys Lys Glu210 215 220
Gln Gln Ile Phe Thr Thr Lys Ser Gln Glu Ser Gln Gln Asp Arg Glu225 230 235 240Asn Arg Asp Gln Lys Gln Asp Arg Gln His Asp Gly Gln His Gln Asp245 250 255Asp Asp Asp Asp Gln Gln Lys Gly Arg Gly Lys Lys Tyr Lys Ser Lys260 265 270Thr Ser Ala Glu Ala Val Pro Ala Asp Leu Ser Val Ala His Leu Arg275 280 285Tyr Leu Asn Glu Val Arg Gln Ser Arg Glu Ile His Val Glu Glu Glu290 295 300Lys Thr Phe Lys Lys Lys Ala Gln Ser Pro Met Ala Leu Phe Ser Ala305 310 315 320Pro Thr Pro Pro Ala Gly Phe Thr Pro Ile Pro Thr Pro Lys Ile Glu325 330 335Asn Val Phe Ile Arg Phe Met Lys Leu Met Glu Arg Ile Leu Gly Gln340 345 350Ala Glu Ala Glu Ala Gln Glu Leu Tyr Leu Arg Val Lys Glu Arg Thr355 360 365Asp Asn Val Asp Thr Leu Met Leu Leu Ile Ser Lys Ile Asn Ser Glu370 375 380Lys Gly Ala Ile Asp Trp Arg Asp Asp Ser Glu Met Lys Ala Leu Val385 390 395 400Asp Gln Ala Lys Lys Leu Gly Val Thr Ile Thr Asp Thr Leu Gln Trp405 410 415Ser Glu Glu Glu Lys Lys Leu Leu Lys Glu Asn Ile Gln Met Arg Lys420 425 430Glu Asn Leu Glu Lys Ile Thr Gln Leu Glu Arg Thr Asp Met Gln Arg435 440 445His Leu Gln Glu Val Ser Gln Cys His Gln Ala Arg Ser Asn Ala Leu450 455 460Lys Leu Leu Lys Glu Leu Met Asp Thr Phe Ile Tyr Asn Leu Arg Pro465 470 475 480
2101312111083212DNA213Chlamydia psittaci400131atgaagaaca aaacactaag cgttttctct ttggtggggt tggtttgttc ggtaggagtt 60ttaccaggca atgaaagttc tctccctgta caacgggagt atccaagtcg tacagaaaga 120atccctgaag atcctgccgg aattgctatt catgatcgtg tgttatttaa aatcgatgaa 180gataacgttg ttacgacttt agatgttata cagaagctta accttctttt tgcttcttca 240taccctcagc ttatggattc ttatcctgcg cgttcgcaat actatacagc catgtggcca 300gtagttttag aatctgtaat agacgagttt cttatggttg ccgatgctaa gactaaaaaa 360atccaggtgg actctactac agtgaatgaa gaaattgaag caatgtttgg tagagatcta 420tctcctttgt atgtacattt tgacatgacc cccgaagatg ttttcaacgt ggtaaatcgt 480accctaattg ctcagagagt catgggtatg atggtgcgct ctaaggtaat gttgaaagtt 540accccaggga aaattcgcga acattataat cagctggctg aagatgcagc aaacactact 600gtatggaaat acagagttgt tacgattaaa gcagctacag agtcattatc gagccaaatt 660gccgataaag tctgcgctag gttaaatgaa acgcaaagct ggaataaaga gcgtttatct 720gctcttactc tttctcaagg agggcagttt gtctgttctg aagagtttac acgcaatgat 780aaagaattat cagaagctca taaaatggaa ttgtcctcgg taaactaccc acaaactatt 840tgcagcctac ctaaagccca taagtcagga cataagcttt atgtgcttct tgataaatct 900gcaatggcaa tgcagcccct agaagaaatg gaaacgcaga taaagcagac gctatttatg 960aattatgctg ggactataga aagtcagtat aaaatgaaat tgcgtacacg ctatggattt 1020gactcttcaa cgattgctaa attgctttct gaagaagctc cgcctctatt ttcattgctt 1080tag1083210132211360212PRT213Chlamydia psittaci400132Met Lys Asn Lys Thr Leu Ser Val Phe Ser Leu Val Gly Leu Val Cys1 5 10 15Ser Val Gly Val Leu Pro Gly Asn Glu Ser Ser Leu Pro Val Gln Arg20 25 30Glu Tyr Pro Ser Arg Thr Glu Arg Ile Pro Glu Asp Pro Ala Gly Ile35 40 45
Ala Ile His Asp Arg Val Leu Phe Lys Ile Asp Glu Asp Asn Val Val50 55 60Thr Thr Leu Asp Val Ile Gln Lys Leu Asn Leu Leu Phe Ala Ser Ser65 70 75 80Tyr Pro Gln Leu Met Asp Ser Tyr Pro Ala Arg Ser Gln Tyr Tyr Thr85 90 95Ala Met Trp Pro Val Val Leu Glu Ser Val Ile Asp Glu Phe Leu Met100 105 110Val Ala Asp Ala Lys Thr Lys Lys Ile Gln Val Asp Ser Thr Thr Val115 120 125Asn Glu Glu Ile Glu Ala Met Phe Gly Arg Asp Leu Ser Pro Leu Tyr130 135 140Val His Phe Asp Met Thr Pro Glu Asp Val Phe Asn Val Val Asn Arg145 150 155 160Thr Leu Ile Ala Gln Arg Val Met Gly Met Met Val Arg Ser Lys Val165 170 175Met Leu Lys Val Thr Pro Gly Lys Ile Arg Glu His Tyr Asn Gln Leu180 185 190Ala Glu Asp Ala Ala Asn Thr Thr Val Trp Lys Tyr Arg Val Val Thr195 200 205Ile Lys Ala Ala Thr Glu Ser Leu Ser Ser Gln Ile Ala Asp Lys Val210 215 220Cys Ala Arg Leu Asn Glu Thr Gln Ser Trp Asn Lys Glu Arg Leu Ser225 230 235 240Ala Leu Thr Leu Ser Gln Gly Gly Gln Phe Val Cys Ser Glu Glu Phe245 250 255Thr Arg Asn Asp Lys Glu Leu Ser Glu Ala His Lys Met Glu Leu Ser260 265 270Ser Val Asn Tyr Pro Gln Thr Ile Cys Ser Leu Pro Lys Ala His Lys275 280 285Ser Gly His Lys Leu Tyr Val Leu Leu Asp Lys Ser Ala Met Ala Met290 295 300Gln Pro Leu Glu Glu Met Glu Thr Gln Ile Lys Gln Thr Leu Phe Met
305 310 315 320Asn Tyr Ala Gly Thr Ile Glu Ser Gln Tyr Lys Met Lys Leu Arg Thr325 330 335Arg Tyr Gly Phe Asp Ser Ser Thr Ile Ala Lys Leu Leu Ser Glu Glu340 345 350Ala Pro Pro Leu Phe Ser Leu Leu355 36021013321134212DNA213Artificial Sequence220
223Synthetic DNA400133agtcaagctt atgattgtac ggacatagag accg 3421013421135212DNA213Artificial Sequence220
223Synthetic DNA400134agtcaagctt gtaggtttat taaaggggat gtacc 3521013521134212DNA213Artificial Sequence220
223Synthetic DNA400135agtcaagctt gtagctgttc ttttcagaga gctt 3421013621133212DNA213Artificial Sequence220
223Synthetic DNA400136agtcaagctt tactttttga aggctagtac gtt 3321013721133
212DNA213Artificial Sequence220
223Synthetic DNA400137agtcaagctt ggaaggaata tcgtttacct gct 3321013821133212DNA213Arti ficial Sequence220
223Synthetic DNA400138agtcaagctt gtagatgccc atcctaccaa cag 3321013921134212DNA213Artificial Sequence220
223Synthetic DNA400139agtcaagctt tccctaaaat gaataaacta agga 3421014021133212DNA213Artificial Sequence220
223Synthetic DNA400140agtcaagctt aagtgtcatt gaaaaggttc agg 3321014121132212DNA213Artificial Sequence220
223Synthetic DNA400141agtcaagctt gccaaccaac ggttagacga aa3221014221134212DNA213Artificial Sequence220
223Synthetic DNA
400142agtcaagctt gcgtgccttt tagaaaattt agca 3421014321135212DNA213Artificial Sequence220
223Synthetic DNA400143agtcaagctt gtaacgactc cttctttcaa cgatt 3521014421133212DNA213Artificial Sequence220
223Synthetic DNA400144agtcaagctt ccattaagga tctaaaacaa ttt 3321014521133212DNA213Artificial Sequence220
223Synthetic DNA400145agtcaagctt tgtttgagat gaattcgcat ttt 3321014621134212DNA213Artificial Sequence220
223Synthetic DNA400146agtcaagctt ttgcatgaat tcgtaaaata gaaa 3421014721135212DNA213Artificial Sequence220
223Synthetic DNA400147agtcaagctt gtagggtttg tggagaaaat tgtta 35
21014821133212DNA213Artificial Sequence220
223Synthetic DNA400148agtcaagctt cttctattag cctgtttcca att 3321014921134212DNA213Artificial Sequence220
223Synthetic DNA400149agtcaagctt ctgttgttga attaatagct tctt 3421015021135212DNA213Artificial Sequence220
223Synthetic DNA400150agtcaagctt gtgagaaaaa caacaattct tatcc 3521015121134212DNA213Artificial Sequence220
223Synthetic DNA400151agtcaagctt acgactcaat taacagtgat gcaa 3421015221134212DNA213Artificial Sequence220
223Synthetic DNA400152agtcaagctt tcaagacaat tagagagaaa gagc 3421015321135212DNA213Artificial Sequence
220
223Synthetic DNA400153agtcaagctt gtaggcattg cttaaaaata aaatg 3521015421135212DNA213Artificial Sequence220
223Synthetic DNA400154agtcaagctt gtaatcggat tttaaaccaa ctttt 3521015521135212DNA213Artificial Sequence220
223Synthetic DNA400155agtcaagctt gtaggaattt ttattacgag tttca 3521015621133212DNA213Artificial Sequence220
223Synthetic DNA400156agtcaagctt caagaagcgt taagaaaacg aaa 3321015721134212DNA213Artificial Sequence220
223Synthetic DNA400157agtcaagctt ttattaaaat ttgttaaagg gagg 3421015821135212DNA213Artificial Sequence220
223Synthetic DNA400158agtcaagctt gtaaactaat tcggattaaa aatga 35
21015921134212DNA213Artificial Sequence220
223Synthetic DNA400159agtcaagctt gaaggaaaag aagttctcat aaag 3421016021132212DNA213Artificial Sequence220
223Synthetic DNA400160agtcaagctt gtagcgtctg gaaaaattct gg3221016121133212DNA213Artificial Sequence220
223Synthetic DNA400161agtcaagctt gggtaagaac accttctaat atg 3321016221132212DNA213Artificial Sequence220
223Synthetic DNA400162gactaagctt gtaatctatt tttagatagg aa3221016321133212DNA213Artificial Sequence220
223Synthetic DNA400163agtcaagctt cccgtaatta ctgtccgtac aac 3321016421131212DNA
213Artificial Sequence220
223Synthetic DNA400164gactaagctt attatataga cagattaaaa t 3121016521132212DNA213Artificial Sequence220
223Synthetic DNA400165agtcaagctt agggttttaa gtcggtttga tg3221016621134212DNA213Artificial Sequence220
223Synthetic DNA400166agtcaagctt tcgataatca taattcaaag cgta 3421016721134212DNA213Artificial Sequence220
223Synthetic DNA400167agtcaagctt tccatgaatt ctaaaatgat tagg 3421016821132212DNA213Artificial Sequence220
223Synthetic DNA400168agtcaagctt aatttaaagg aactcaggtg aa3221016921133212DNA213Artificial Sequence220
223Synthetic DNA
400169agtcaagctt gtaacatggg gtatggaaag acc 3321017021133212DNA213Artificial Sequence220
223Synthetic DNA400170agtcaagctt gtaagcacct gctctcgaat aca 3321017121134212DNA213Artificial Sequence220
223Synthetic DNA400171agtcaagctt gtaacggtct ctttgcttgt tttt 3421017221134212DNA213Artificial Sequence220
223Synthetic DNA400172agtcaagctt gtaacgatcc gaacttatcg tagt 3421017321134212DNA213Artificial Sequence220
223Synthetic DNA400173agtcaagctt gtaaattcat gggcctaatg ataa 3421017421134212DNA213Artificial Sequence220
223Synthetic DNA400174agtcaagctt gtaattgggg aatctctggg agac 34210175
21132212DNA213Artificial Sequence220
223Synthetic DNA400175agtcaagctt gtaatggagg attggctaag ga3221017621135212DNA213Artificial Sequence220
223Synthetic DNA400176agtcaagctt gtaagaggga ccttacctat tgctt 3521017721131212DNA213Artificial Sequence220
223Synthetic DNA400177agtcaagctt gcggaatggt taaaggaaac g 3121017821133212DNA213Artificial Sequence220
223Synthetic DNA400178agtcaagctt gtaaaaccag cctagccttc aaa 3321017921134212DNA213Artificial Sequence220
223Synthetic DNA400179agtcaagctt gtaacctgct tctttaggaa ctac 3421018021134212DNA213Artificial Sequence220
223Synthetic DNA400180agtcaagctt gtaaaattca gcacaggctt gtaa 3421018121132212DNA213Artificial Sequence220
223Synthetic DNA400181gactaagctt gtaaattgga gattgtagta gc3221018221132212DNA213Artificial Sequence220
223Synthetic DNA400182atgcaagctt acgagcaaga gcataaaatc ca3221018321133212DNA213Artificial Sequence220
223Synthetic DNA400183agtcaagctt gaattgctaa cagacactaa agg 3321018421135212DNA213Artificial Sequence220
223Synthetic DNA400184agtcaagctt gttttaaaaa gccttgtaag gaggt 3521018521136212DNA213Artificial Sequence220
223Synthetic DNA400185agtcaagctt gtaagaatta ccataaatca gaggaa36
21018621136212DNA213Artificial Sequence220
223Synthetic DNA400186agtcaagctt gtaacagaag gaaaagcata ccactg3621018721133212DNA213Artificial Sequence220
223Synthetic DNA400187agtcaagctt gccataagtc gtttacaaga tcg 3321018821131212DNA213Artificial Sequence220
223Synthetic DNA400188agtcaagctt gtgaatcagg gggaagctag t 3121018921132212DNA213Artificial Sequence220
223Synthetic DNA400189agtcaagctt cgcaagatga tataaaggtc ca3221019021134212DNA213Artificial Sequence220
223Synthetic DNA400190agtcaagctt taaataacca agtattgggg ttta 3421019121134212DNA213Artificial Sequence
220
223Synthetic DNA400191agtcaagctt gtagacatgg ctgtcagatc ttgg 3421019221133212DNA213Artificial Sequence220
223Synthetic DNA400192agtcaagctt ctctttttaa agacaacgcg aat 3321019321135212DNA213Artificial Sequence220
223Synthetic DNA400193agtcaagctt gtaattaatc ttgcggagat gttgg 3521019421133212DNA213Artificial Sequence220
223Synthetic DNA400194agtcaagctt gtaagcctgc ttggtcttct gtt 3321019521134212DNA213Artificial Sequence220
223Synthetic DNA400195atgcaagctt gtaagggaag atgtccgttc agtt 3421019621134212DNA213Artificial Sequence220
223Synthetic DNA400196
agtcaagctt atggctatga agagcaattt attc 3421019721130212DNA213Artificial Sequence220
223Synthetic DNA400197agtcaagctt gtaactcccg caccaacctc 3021019821135212DNA213Artificial Sequence220
223Synthetic DNA400198agtcaagctt gtaatctggt taacacatgc tgagg 3521019921135212DNA213Artificial Sequence220
223Synthetic DNA400199agtcaagctt gtaagagccg agcatagata gaaac 3521020021134212DNA213Artificial Sequence220
223Synthetic DNA400200agtcaagctt ttaataagct gaataaacca atga 3421020121134212DNA213Artificial Sequence220
223Synthetic DNA400201agtcaagctt gtaacatgac gacatcacct tatt 3421020221134
212DNA213Arti ficial Sequence220
223Synthetic DNA400202agtcaagctt gtaagtcagg aagtccgtgg tgat 3421020321132212DNA213Artificial Sequence220
223Synthetic DNA400203agtcaagctt gtaatttgcg gttgggaaat aa3221020421134212DNA213Artificial Sequence220
223Synthetic DNA400204agtcaagctt gtaacccttg cctctatttt gaga 3421020521137212DNA213Artificial Sequence220
223Synthetic DNA400205agtcaagctt gtaattgcga gaaggattaa atcttag 3721020621132212DNA213Artificial Sequence220
223Synthetic DNA400206agtcaagctt atctcaaaca tcaagtgctg aa3221020721131212DNA213Artificial Sequence220
223Synthetic DNA
400207agtcaagctt gtaagcctcg tcaaatcctg a 3121020821131212DNA213Artificial Sequence220
223Synthetic DNA400208agtcaagctt tcttcaggaa ctttaaaaac a 3121020921133212DNA213Artificial Sequence220
223Synthetic DNA400209agtcaagctt taatagagct cattggagga gga 3321021021136212DNA213Artificial Sequence220
223Synthetic DNA400210agtcaagctt gtaatgtaga aaagcgcaga gagaaa3621021121135212DNA213Artificial Sequence220
223Synthetic DNA400211agtcaagctt gtaatcgaaa accagttagg ttgaa 3521021221134212DNA213Artificial Sequence220
223Synthetic DNA400212agtcaagctt gtaatgggtc ctaatggagg cttt 34
21021321132212DNA213Artificial Sequence220
223Synthetic DNA400213agtcaagctt gacctccttg taaccattct cg3221021421133212DNA213Artificial Sequence220
223Synthetic DNA400214agtcaagctt tccttaagag gataaatcca cag 3321021521133212DNA213Artificial Sequence220
223Synthetic DNA400215agtcaagctt ttcgaaaaca taaataatgt gga 3321021621133212DNA213Artificial Sequence220
223Synthetic DNA400216agtcaagctt gtgcaggaaa atgatgtttt agc 3321021721130212DNA213Artificial Sequence220
223Synthetic DNA400217agtcaagctt cagcctggtt tcgtaatttt 3021021821136212DNA213Artificial Sequence
220
223Synthetic DNA400218agtcaagctt gtaaaccaca ccaatattat ttagga3621021921134212DNA213Artificial Sequence220
223Synthetic DNA400219agtcaagctt gtaacctggg caggtcaaaa tcta 3421022021134212DNA213Artificial Sequence220
223Synthetic DNA400220agtcaagctt gtaatgggtt tccggtttca atca 3421022121135212DNA213Artificial Sequence220
223Synthetic DNA400221agtcaagctt gtaagccaag agcaggataa aacga 3521022221135212DNA213Artificial Sequence220
223Synthetic DNA400222agtcaagctt gtaaattttt cctataagct ggttc 3521022321135212DNA213Artificial Sequence220
223Synthetic DNA400223agtcaagctt ttgataagaa attagccaac atagg 35
21022421133212DNA213Artificial Sequence220
223Synthetic DNA400224agtcaagctt ttcggtttaa gtaatagaag tgg 3321022521135212DNA213Artificial Sequence220
223Synthetic DNA400225agtcaagctt gtaagacggt gccttcaata acaaa 3521022621133212DNA213Artificial Sequence220
223Synthetic DNA400226agtcaagctt gtaaaaacag ggaaagacga tga 3321022721132212DNA213Artificial Sequence220
223Synthetic DNA400227agtcaagctt gtaagcggtt acgtggagtc aa3221022821133212DNA213Artificial Sequence220
223Synthetic DNA400228agtcaagctt gtaatccgga gcctttctcc tat 3321022921134212DNA
213Artificial Sequence220
223Synthetic DNA400229agtcaagctt gtaaagatga atgattccag aaag 3421023021133212DNA213Artificial Sequence220
223Synthetic DNA400230agtcaagctt ggactaagta aaacggagca gga 3321023121134212DNA213Artificial Sequence220
223Synthetic DNA400231agtcaagctt ttcgaggttt atttaattct tcca 3421023221132212DNA213Artificial Sequence220
223Synthetic DNA400232agtcaagctt acgtatcaac cgtaaaatgg tg3221023321132212DNA213Artificial Sequence220
223Synthetic DNA400233agtcaagctt gtaagcaaaa acaacgggaa tc3221023421136212DNA213Artificial Sequence220
223Synthetic DNA
400234agtcaagctt gtaaatgaag agtgctgtgt taaagt3621023521134212DNA213Artificial Sequence220
223Synthetic DNA400235agtcaagctt gtaacacaat cttgggcaag agac 3421023621136212DNA213Artificial Sequence220
223Synthetic DNA400236agtcaagctt gtaagagaag agaaaggcta aggatg3621023721131212DNA213Artificial Sequence220
223Synthetic DNA400237agtcaagctt ataaacccct gtcttaaccc a 3121023821130212DNA213Artificial Sequence220
223Synthetic DNA400238agtcaagctt tcctttctaa aaggcctgga 3021023921135212DNA213Artificial Sequence220
223Synthetic DNA400239agtcaagctt gcaaatctaa ggagttaggt taggg 35210240
21132212DNA213Artificial Sequence220
223Synthetic DNA400240agtcaagctt gtaatacagg atggtgccca ct3221024121129212DNA213Artificial Sequence220
223Synthetic DNA400241agtcaagctt gcgcaaataa acgatacaa2921024221133212DNA213Artificial Sequence220
223Synthetic DNA400242agtcaagctt cattgtgtta taattgcagg cta 3321024321131212DNA213Artificial Sequence220
223Synthetic DNA400243agtcaagctt tgacgcccct taaaataaag a 3121024421130212DNA213Artificial Sequence220
223Synthetic DNA400244agtcaagctt ccgcaaaatg gtttaacaga 3021024521136212DNA213Artificial Sequence220
223Synthetic DNA400245agtcaagctt gtaagcttgg gaatctacac attttt3621024621133212DNA213Artificial Sequence220
223Synthetic DNA400246agtcaagctt gtaaaagctg aataagccca tga 3321024721133212DNA213Artificial Sequence220
223Synthetic DNA400247agtcaagctt gtaaccgtga ggagtctgaa caa 3321024821135212DNA213Artificial Sequence220
223Synthetic DNA400248agtcaagctt ttaatcgaag aaaataggta ggaaa 3521024921135212DNA213Artificial Sequence220
223Synthetic DNA400249agtcaagctt gtaacgcttc tacatcccca taatt 3521025021129212DNA213Artificial Sequence220
223Synthetic DNA400250agtctctaga tgcttttcgg accatagtc29
21025121131212DNA213Artificial Sequence220
223Synthetic DNA40025lagtctctaga atcctcttcc caaggaatca g 3121025221130212DNA213Artificial Sequence220
223Synthetic DNA400252agtctctaga tcgaatcgat ttggaaggag 3021025321133212DNA213Artificial Sequence220
223Synthetic DNA400253agtctctaga ttcaataatg ccagagcttt ttc 3321025421130212DNA213Artificial Sequence220
223Synthetic DNA400254agtctctaga ttcatccacc caaatagcac 3021025521129212DNA213Artificial Sequence220
223Synthetic DNA400255agtctctaga agtaagaggg agcccagga2921025621130212DNA213Artificial Sequence
220
223Synthetic DNA400256agtctctaga tgacggggga ggtgtattag 3021025721131212DNA213Artificial Sequence220
223Synthetic DNA400257agtctctaga taggaggcct ttcgattgtt t 3121025821129212DNA213Artificial Sequence220
223Synthetic DNA400258agcttctaga tggaaaggat acggattgg2921025921130212DNA213Artificial Sequence220
223Synthetic DNA400259agtctctaga gaatggggga atacaaatca 3021026021130212DNA213Artificial Sequence220
223Synthetic DNA400260agtctctaga ggcgagagaa agtttcgtta 3021026121129212DNA213Artificial Sequence220
223Synthetic DNA400261
agtctctaga caacttgttc atgcgacag2921026221130212DNA213Artificial Sequence220
223Synthetic DNA400262agcttctaga cgcatccgaa agacttttct 3021026321132212DNA213Artificial Sequence220
223Synthetic DNA400263agtctctaga acgttctaag ccgtaattct cg3221026421130212DNA213Artificial Sequence220
223Synthetic DNA400264agtctctaga tgcggggatc gtataagcta 3021026521131212DNA213Artificial Sequence220
223Synthetic DNA400265agtctctaga aggtgattgc ttcccaagtc t 3121026621131212DNA213Artificial Sequence220
223Synthetic DNA400266agtctctaga ataagagtcg agggcttgca c 3121026721132
212DNA213Artificial Sequence220
223Synthetic DNA400267agtctctaga tgtaggaata cctggagtcg tg3221026821131212DNA213Artificial Sequence220
223Synthetic DNA400268agtctctaga gcgctgagta gcgtcgtgta a 3121026921131212DNA213Artificial Sequence220
223Synthetic DNA400269agcttctaga gaattcaaga tgatccaaac a 3121027021130212DNA213Artificial Sequence220
223Synthetic DNA400270agtctctaga agtcttatga agcaaagcag 3021027121133212DNA213Artificial Sequence220
223Synthetic DNA400271agtctctaga atttatatgc cacgccttct ttc 3321027221129212DNA213Artificial Sequence220
223Synthetic DNA
400272agtctctaga ggaggtttcc gagacgatt2921027321130212DNA213Artificial Sequence220
223Synthetic DNA400273agtctctaga atcccagtcg tgagaagcat 3021027421134212DNA213Artificial Sequence220
223Synthetic DNA400274agtctctaga gagaagaata tctgcaacta gagc 3421027521130212DNA213Artificial Sequence220
223Synthetic DNA400275agtctctaga ggtaacagtg catgcgaaag 3021027621132212DNA213Artificial Sequence220
223Synthetic DNA400276agtctctaga caaaatagga atagcagctt gg3221027721133212DNA213Arti ficial Sequence220
223Synthetic DNA400277agtctctaga cggtcttaaa ctacttttca atg 33
21027821132212DNA213Artificial Sequence220
223Synthetic DNA400278agtctctaga atcaaccaca tcttctggac aa3221027921131212DNA213Artificial Sequence220
223Synthetic DNA400279gacttctaga tccaggtttt tcggaagcag a 3121028021128212DNA213Artificial Sequence220
223Synthetic DNA400280agtctctaga agtagacggg agttgctg 2821028121131212DNA213Artificial Sequence220
223Synthetic DNA400281gacttctaga cttaaaaaat acccaggaac a 3121028221130212DNA213Artificial Sequence220
223Synthetic DNA400282agtctctaga ccgaatcgct tcattcgtag 3021028321132212DNA213Artificial Sequence
220
223Synthetic DNA400283agtctctaga aaggatcaga tgtgctttag gg3221028421130212DNA213Artificial Sequence220
223Synthetic DNA400284agtctctaga cgaaaattgg gtagcctgac 3021028521130212DNA213Artificial Sequence220
223Synthetic DNA400285agtctctaga atcaaagttg ctgcaggatt 3021028621130212DNA213Artificial Sequence220
223Synthetic DNA400286agtctctaga ctcggcatcc ttttgttttg 3021028721130212DNA213Artificial Sequence220
223Synthetic DNA400287agtctctaga ttcttcttgg tgcctgctaa 3021028821130212DNA213Artificial Sequence220
223Synthetic DNA400288agtctctaga agtgcagctg gatagggttg 30
21028921130212DNA213Artificial Sequence220
223Synthetic DNA400289agtctctaga tccggatgcg gtagtagttg 3021029021131212DNA213Artificial Sequence220
223Synthetic DNA400290agtctctaga taaccctgtg gatgacgttt t 3121029121130212DNA213Artificial Sequence220
223Synthetic DNA400291agtctctaga gggactaacg acctcagcac 3021029221130212DNA213Artificial Sequence220
223Synthetic DNA400292agtctctaga aacaccaccg acatcacaaa 3021029321130212DNA213Artificial Sequence220
223Synthetic DNA400293agtctctaga aatgaggacc tctccttcgt 3021029421131212DNA
213Artificial Sequence220
223Synthetic DNA400294agtctctaga ttgtccatca aagcctacaa t 3121029521129212DNA213Artificial Sequence220
223Synthetic DNA400295agtctctaga gctttttgca taggggaag2921029621131212DNA213Artificial Sequence220
223Synthetic DNA400296agtctctaga ttgtagacca gcggtgacac t 3121029721130212DNA213Artificial Sequence220
223Synthetic DNA400297agtctctaga gcgttctgga gtgacgaaac 3021029821131212DNA213Artificial Sequence220
223Synthetic DNA400298gacttctaga aacaattgta tgattccatc c 3121029921130212DNA213Artificial Sequence220
223Synthetic DNA
400299atgctctaga cctttggcgg actttttctt 3021030021130212DNA213Artificial Sequence220
223Synthetic DNA400300agtctctaga tacccattct tgaccggaaa 3021030121130212DNA213Artificial Sequence220
223Synthetic DNA400301agtctctaga ctgagcaaag gagctgacag 3021030221132212DNA213Artificial Sequence220
223Synthetic DNA400302agtctctaga agcatcagtg caatgaggat aa3221030321129212DNA213Artificial Sequence220
223Synthetic DNA400303agtctctaga tccaaaacga tcctgttcc2921030421130212DNA213Artificial Sequence220
223Synthetic DNA400304agtctctaga ttccacacaa gagaccacca 30210305
21130212DNA213Artificial Sequence220
223Synthetic DNA400305agtctctaga tcgtgtttgc tttccttcca 3021030621130212DNA213Artificial Sequence220
223Synthetic DNA400306agtctctaga gacagtgcct tgtgttgatg 3021030721130212DNA213Artificial Sequence220
223Synthetic DNA400307agtctctaga tcgaagcagc gcatgtaact 3021030821131212DNA213Artificial Sequence220
223Synthetic DNA400308agtctctaga agagtcgcgt cctatagacc a 3121030921130212DNA213Artificial Sequence220
223Synthetic DNA400309agcttctaga cgtgtgagtc ccctgaactt 3021031021132212DNA213Artificial Sequence220
223Synthetic DNA400310agtctctaga ctctgtagtc agctggtcgt tg3221031121131212DNA213Artificial Sequence220
223Synthetic DNA400311agtctctaga cgagagagga agttcagcgt a 3121031221131212DNA213Artificial Sequence220
223Synthetic DNA400312atgctctaga agctccctta acgacttctg g 3121031321132212DNA213Artificial Sequence220
223Synthetic DNA400313agtctctaga agggtaacac actaggggaa ga3221031421131212DNA213Artificial Sequence220
223Synthetic DNA400314agtctctaga tccttcagac caacgctgat a 3121031521130212DNA213Artificial Sequence220
223Synthetic DNA400315agtctctaga tgacaggccg tttctatcaa 30
21031621129212DNA213Artificial Sequence220
223Synthetic DNA400316agtctctaga gcctgcaatc aatccctgt2921031721129212DNA213Artificial Sequence220
223Synthetic DNA400317agtctctaga ttctgcggaa ggaagctgt2921031821130212DNA213Artificial Sequence220
223Synthetic DNA400318agtctctaga agcggcagaa aatgagaaaa 3021031921133212DNA213Artificial Sequence220
223Synthetic DNA400319agtctctaga caaagcaatt agagtgaacg aca 3321032021130212DNA213Artificial Sequence220
223Synthetic DNA400320agtctctaga agcacaggca caacgtttac 3021032121130212DNA213Artificial Sequence
220
223Synthetic DNA400321agtctctaga cggtaccaag ggccattttg 3021032221130212DNA213Artificial Sequence220
223Synthetic DNA400322agtctctaga aagatgtcgg gaaagtgtcg 3021032321130212DNA213Artificial Sequence220
223Synthetic DNA400323agtctctaga ttggcttgcc ttatcattcg 3021032421129212DNA213Artificial Sequence220
223Synthetic DNA400324agtctctaga gacgacttgc ttgctcact2921032521130212DNA213Arti ficial Sequence220
223Synthetic DNA400325agtctctaga tgctgcgaca cgaatttcta 3021032621131212DNA213Artificial Sequence220
223Synthetic DNA400326
agtctctaga gaggagggaa acaccgttaa t 3121032721129212DNA213Artificial Sequence220
223Synthetic DNA400327agtctctaga cccttccaat tgaggaaaa2921032821130212DNA213Artificial Sequence220
223Synthetic DNA400328agtctctaga tgcaatgata tcgtcagcag 3021032921130212DNA213Artificial Sequence220
223Synthetic DNA400329agtctctaga cacctggatt ttgccttcag 3021033021129212DNA213Artificial Sequence220
223Synthetic DNA400330agtctctaga cttccatggt aaaggagca2921033121130212DNA213Artificial Sequence220
223Synthetic DNA400331agtctctaqa ttctgcagca acaacagctt 3021033221130
212DNA213Artificial Sequence220
223Synthetic DNA400332agtctctaga agcttcttga ggcgctacag 3021033321131212DNA213Artificial Sequence220
223Synthetic DNA400333agtctctaga tccagaggct gtggaaactg t 3121033421129212DNA213Artificial Sequence220
223Synthetic DNA400334agtctctaga agcatgaggc tgtatgagg2921033521132212DNA213Artificial Sequence220
223Synthetic DNA400335agtctctaga tgcttgtaga agagcagaat cg3221033621131212DNA213Artificial Sequence220
223Synthetic DNA400336agtctctaga cccagatgaa gtcactggaa c 3121033721131212DNA213Artificial Sequence220
223Synthetic DNA
400337agtctctaga acggatttct tcctggtgtc t 3121033821131212DNA213Artificial Sequence220
223Synthetic DNA400338agtctctaga tgatatgctc ccattctttc g 3121033921131212DNA213Artificial Sequence220
223Synthetic DNA400339agtctctaga tccttcgtat acgccagtac c 3121034021130212DNA213Artificial Sequence220
223Synthetic DNA400340agtctctaga gcatccacgt ttgttccatt 3021034121130212DNA213Artificial Sequence220
223Synthetic DNA400341agtctctaga agcattccct gtaccagacc 3021034221130212DNA213Artificial Sequence220
223Synthetic DNA400342agtctctaga agccgaacga gctaagacat 30
21034321130212DNA213Artificial Sequence220
223Synthetic DNA400343agtctctaga gaccaaagct ccctcttgaa 3021034421130212DNA213Artificial Sequence220
223Synthetic DNA400344agtctctaga ggcatacgcc tgtaattgct 3021034521127212DNA213Artificial Sequence220
223Synthetic DNA400345agtctctaga ttcttcaggg ccagcaa 2721034621130212DNA213Artificial Sequence220
223Synthetic DNA400346agtctctaga aggaaagcct atcgcacaca 3021034721131212DNA213Artificial Sequence220
223Synthetic DNA400347agtctctaga agaccaactc gtcccatttt c 3121034821130212DNA213Artificial Sequence
220
223Synthetic DNA400348agtctctaga gtgggctttg gttacatcgt 3021034921130212DNA213Artificial Sequence220
223Synthetic DNA400349agtctctaga agttccttgc gtggatgttt 3021035021131212DNA213Artificial Sequence220
223Synthetic DNA400350agtctctaga catccgatct gctttctctt g 3121035121130212DNA213Artificial Sequence220
223Synthetic DNA400351agtctctaga atgggagaga gcttcttgct 3021035221131212DNA213Artificial Sequence220
223Synthetic DNA400352agtctctaga gatctccagc ttaagggttg c 3121035321131212DNA213Artificial Sequence220
223Synthetic DNA400353agtctctaga agggttctcc tcaagttcat c 31
21035421130212DNA213Artificial Sequence220
223Synthetic DNA400354agtctctaga cgttgcagag ttcgttgttc 3021035521130212DNA213Artificial Sequence220
223Synthetic DNA400355agcttctaga cagaacaacg tctcctacgc 3021035621130212DNA213Artificial Sequence220
223Synthetic DNA400356agcttctaga agccaaacgc atagcttcat 3021035721130212DNA213Artificial Sequence220
223Synthetic DNA400357agtctctaga tagaagttgc aggcgttcct 3021035821132212DNA213Artificial Sequence220
223Synthetic DNA400358agtctctaga tccgtagttg ttacggaagg tt3221035921130212DNA
213Artificial Sequence220
223Synthetic DNA400359agtctctaga tttcttggct ccctgcatag 3021036021130212DNA213Artificial Sequence220
223Synthetic DNA400360agtctctaga cgcagaatgg gataggacat 3021036121130212DNA213Artificial Secquence220
223Synthetic DNA400361agtctctaga ttgcacacct tggacgtact 3021036221134212DNA213Artificial Sequence220
223Synthetic DNA400362agtctctaga tttcgttaat tctcccttag acca 3421036321130212DNA213Artificial Sequence220
223Synthetic DNA400363agtctctaga aagtttctgt gtcgctacgg 3021036421131212DNA213Artificial Sequence220
223Synthetic DNA
400364agtctctaga agaaaaatcc atgggctgta a 3121036521130212DNA213Artificial Sequence220
223Synthetic DNA400365agtctctaga ttcatcggct gttgctgtat 3021036621130212DNA213Artificial Sequence220
223Synthetic DNA400366agtctctaga gaatacggaa agcgcaacat 3021036721127212DNA213Artificial Sequence220
223Synthetic DNA400367atcgtaccat ggagcaaccc aattgtg 2721036821138212DNA213Artificial Sequence220
223Synthetic DNA400368atcgtaccat ggaattaaat aaaacatccg agtctttg 3821036921133212DNA213Artificial Sequence220
223Synthetic DNA400369atcgtaccat ggttctcgct tcttgtcttc ttg 33210370
21135212DNA213Artificial Sequence220
223Synthetic DNA400370atcgtaccat ggatgaaggt attcaaaccg tttct 3521037121133212DNA213Artificial Sequence220
223Synthetic DNA400371atcgtaccat ggggttctct tctccagcac ttc 3321037221136212DNA213Artificial Sequence220
223Synthetic DNA400372atcgtaccat ggagaacaaa acactaagcg ttttct3621037321131212DNA213Artificial Sequence220
223Synthetic DNA400373agtcggtacc agcttcctta acttcgctga g 3121037421132212DNA213Artificial Sequence220
223Synthetic DNA400374agtcggtacc ttggttttgt tcttgatctt gc3221037521137212DNA213Artificial Sequence220
223Synthetic DNA400375agtcggtacc atctaagaaa tcatcttctg taaggac 3721037621133212DNA213Artificial Sequence220
223Synthetic DNA400376agtcggtacc gtctctagga actagcgttt cca 3321037721135212DNA213Artificial Sequence220
223Synthetic DNA400377agtcggtacc aatgtttgct atcaaactta caatt 3521037821129212DNA213Artificial Sequence220
223Synthetic DNA400378agtcggtacc caatgaaaat agaggcgga29
權利要求
1.一種純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種肺炎衣原體(Chlamydia pneumoniae)蛋白或其片段具有同源性，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成。
2.權利要求1的多肽，其中所述衣原體多肽是選自如下一組中的一或多種肺炎衣原體蛋白或其片段，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成。
3.權利要求1的多肽，其中所述衣原體多肽是選自如下一組中的一或多種肺炎衣原體蛋白或其片段，所述的組由CPn0490、CPn0595、CPn0671、CPn0712、CPn0725、CPn0761、CPn0770、CPn0774、CPn0859、CPn0906、CPn1002和CPn1005組成，或者其中所述衣原體多肽與選自如下一組中的一或多種肺炎衣原體蛋白或其片段具有同源性，所述的組由CPn0490、CPn0595、CPn0671、CPn0712、CPn0725、CPn0761、CPn0770、CPn0774、CPn0859、CPn0906、CPn1002和CPn1005組成。
4.權利要求1的多肽，其中所述同源性衣原體多肽是肺炎衣原體蛋白。
5.權利要求1的多肽，其中所述同源性衣原體多肽是選自如下一組中的沙眼衣原體(Chlamydia trachomatis)蛋白或其片段，所述的組由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848組成。
6.權利要求1的多肽，其中所述同源性衣原體多肽是選自如下一組中的鸚鵡熱衣原體(Chlamydia psittaci)蛋白或其片段，所述的組由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058組成。
7.權利要求1的多肽，其中所述多肽是依據其被一種革蘭氏陰性細菌菌株表達並被所述菌株的III型分泌途徑分泌而鑑別的。
8.權利要求1的多肽，其中含有III型分泌途徑的所述革蘭氏陰性菌株是志賀氏菌屬(Shigella)菌株。
9.權利要求1的多肽，其中所述多肽是依據一種用於鑑別由衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供一種重組表達載體，所述重組表達載體至少含有一種編碼感興趣的多肽的多核苷酸；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述多核苷酸表達產物的分泌；其中所述表達產物的分泌說明其相當於一種分泌型衣原體多肽。
10.權利要求9的多肽，其中所述多肽來自肺炎衣原體。
11.權利要求9的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是一種志賀氏菌屬菌株。
12.權利要求1的多肽，其中所述多肽是依據一種用於鑑別由衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供一種重組表達載體，所述重組表達載體至少包含融合於一種報導基因的編碼所述感興趣的多肽的DNA；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述報導基因表達產物的分泌；其中所述表達產物的分泌說明該融合的DNA至少含有一種相應於分泌型衣原體多肽的多核苷酸。
13.權利要求12的多肽，其中所述多肽來自肺炎衣原體。
14.權利要求12的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
15.編碼至少一種權利要求1的多肽的純化的多核苷酸，或與所述編碼至少一種權利要求1的多肽的純化的多核苷酸在嚴格性條件下雜交的純化的多核苷酸。
16.權利要求15的純化的多核苷酸，其中所述多核苷酸是一種引物或探針。
17.一種免疫原性組合物，其包含至少一種權利要求1的多肽或其免疫原性片段。
18.一種針對衣原體感染的預防接種組合物，其中所述組合物包含至少一種權利要求1的多肽或其免疫原性片段以及一種藥物學可接受的載體。
19.權利要求18的預防接種組合物，其中所述多肽來自肺炎衣原體。
20.權利要求18的預防接種組合物，其中所述感染促成動脈粥樣硬化。
21.權利要求18的預防接種組合物，其中所述感染是性傳播疾病。
22.權利要求18的預防接種組合物，其中所述感染是呼吸道疾病。
23.權利要求18的預防接種組合物，其中所述呼吸道疾病是支氣管炎。
24.一種複合體，其包含權利要求1的多肽以及一種針對所述多肽的抗體。
25.一種複合體，其包含權利要求2的多肽以及一種針對所述多肽的抗體。
26.一種抗衣原體的抗體，其中所述抗體針對權利要求1的多肽或其免疫原性片段。
27.權利要求26的抗體，其中所述多肽來自肺炎衣原體。
28.一種診斷動物衣原體感染的方法，其中所述方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)將所述樣品與權利要求24的抗體相接觸；以及(c)檢測抗原抗體的複合；其中所述複合是表明所述動物存在衣原體感染的指徵。
29.權利要求28的方法，其中所述衣原體感染是肺炎衣原體感染。
30.一種預防或治療動物衣原體感染的方法，其包含給有需要的動物施用有效量的權利要求26的抗體或其免疫原性片段。
31.權利要求30的方法，其中所述衣原體感染是肺炎衣原體感染。
32.一種診斷動物衣原體感染的方法，其中所述方法包含(a)提供一種權利要求1的多肽或其免疫原性片段，所述多肽或片段任選地被標記；(b)將所述多肽或其免疫原性片段與所述動物的血清樣品相接觸；以及(c)檢測所述多肽或其免疫原性片段與血清樣品中含有的抗體之間形成的複合體；其中所述複合體是表明所述動物存在衣原體感染的指徵。
33.權利要求32的方法，其中所述衣原體感染是肺炎衣原體感染。
34.一種檢測動物體內的衣原體的方法，其中所述方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)在所述組織樣品中加入相應於權利要求1的多肽的引物對；(c)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(d)根據所述多核苷酸的存在與否來檢測衣原體的存在。
35.一種篩選抑制分泌型衣原體多肽的分泌的活性分子的方法，其包含(a)將活性分子提供給衣原體培養物；(b)在適合所述活性分子對所述培養物發揮活性的條件下生長或孵育所述培養物一段時間；(c)在所述培養物中加入相應於權利要求1的多肽的引物對；(d)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(e)根據所述多核苷酸的存在與否來檢測分泌型衣原體多肽的存在。
36.用於表達分泌型衣原體多肽的質粒，其中所述質粒至少含有編碼權利要求1的多肽的多核苷酸。
37.權利要求36的質粒，其中所述多肽來自肺炎衣原體。
38.權利要求36的質粒，其中所述多核苷酸還融合於一種報導基因。
39.權利要求38的質粒，其中於2000年12月13日保藏於C.N.C.M.的、保藏號為I-2593的載體被用於構建所述質粒。
40.一種重組革蘭氏陰性菌株，其中所述菌株由權利要求36的質粒轉化。
41.權利要求40的重組革蘭氏陰性菌株，其中所述菌株是一種志賀氏菌屬菌株。
42.一種預防或治療動物衣原體感染的方法，其包含施用有效量的純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種肺炎衣原體蛋白或其片段具有同源性，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成。
43.權利要求42的方法，其中所述衣原體多肽是選自如下一組中的一或多種肺炎衣原體蛋白或其片段，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成。
44.權利要求42的方法，其中所述衣原體感染是肺炎衣原體感染。
45.權利要求42的方法，其中所述衣原體多肽是依據其被一種含有III型分泌途徑的革蘭氏陰性細菌菌株分泌而鑑別的。
46.權利要求42的方法，其中所述動物選自人、馬、牛、豬、山羊、綿羊、鳥、狗和貓。
47.權利要求42的方法，其中所述動物是人。
48.一種可通過III型分泌途徑而獲得的純化的分泌型衣原體多肽，其中所述衣原體多肽是選自如下一組中的一或多種肺炎衣原體蛋白或其片段，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成；或者所述衣原體多肽是被針對選自如下一組中的一或多種肺炎衣原體蛋白或其片段的抗體所識別的多肽，所述的組由CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058組成。
49.一種純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種沙眼衣原體蛋白或其片段具有同源性，所述的組由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848組成。
50.權利要求49的多肽，其中所述同源性衣原體多肽是沙眼衣原體蛋白。
51.權利要求49的多肽，其中所述多肽是依據其被一種革蘭氏陰性細菌菌株表達並被所述菌株的III型分泌途徑分泌而鑑別的。
52.權利要求49的多肽，其中所述衣原體多肽是選自如下一組中的一或多種沙眼衣原體蛋白或其片段，所述的組由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848組成。
53.權利要求49的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
54.權利要求49的多肽，其中所述多肽是依據一種用於鑑別由沙眼衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供一種重組表達載體，所述重組表達載體至少含有一種編碼感興趣的多肽的多核苷酸；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述多核苷酸表達產物的分泌；其中所述表達產物的分泌說明其相應於一種分泌型沙眼衣原體多肽。
55.權利要求54的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是一種志賀氏菌屬菌株。
56.權利要求49的多肽，其中所述多肽是依據一種用於鑑別由沙眼衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供一種重組表達載體，所述重組表達載體至少包含融合於一種報導基因的編碼所述感興趣的多肽的DNA；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述報導基因表達產物的分泌；其中所述表達產物的分泌說明該融合的DNA至少含有一種相應於分泌型沙眼衣原體多肽的多核苷酸。
57.權利要求56的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
58.編碼至少一種權利要求49的多肽的純化的多核苷酸，或與所述編碼至少一種權利要求49的多肽的純化的多核苷酸在在嚴格性條件下雜交的純化的多核苷酸。
59.權利要求58的純化的多核苷酸，其中所述多核苷酸是引物或探針。
60.一種免疫原性組合物，其至少包含權利要求49的多肽或其免疫原性片段。
61.一種針對沙眼衣原體感染的預防接種組合物，其中所述組合物包含至少一種權利要求49的多肽或其免疫原性片段以及一種藥物學可接受的載體。
62.權利要求61的預防接種組合物，其中所述感染是性傳播疾病。
63.一種抗沙眼衣原體的抗體，其中所述抗體針對權利要求49的多肽或其免疫原性片段。
64.一種診斷動物沙眼衣原體感染的方法，其中所述方法包含(a)提供一種疑似感染了沙眼衣原體的動物組織樣品；(b)將所述樣品與權利要求63的抗體相接觸；以及(c)檢測抗原抗體的複合；其中所述複合是表明所述動物存在沙眼衣原體感染的指徵。
65.一種預防或治療動物衣原體感染的方法，其包含給有需要的動物施用有效量的權利要求63的抗體或其免疫原性片段。
66.一種診斷動物的沙眼衣原體感染的方法，其中所述方法包含(a)提供權利要求49的多肽或其免疫原性片段，任選地被標記；(b)將所述多肽或其免疫原性片段與所述動物的血清樣品相接觸；以及(c)檢測所述多肽或其免疫原性片段與血清樣品中含有的抗體之間形成的複合體；其中所述複合體是表明所述動物存在沙眼衣原體感染的指徵。
67.一種檢測動物體內的衣原體的方法，其中所述方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)在所述組織樣品中加入相應於權利要求49的多肽的引物對；(c)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(d)根據所述多核苷酸的存在與否來檢測衣原體的存在。
68.一種篩選抑制分泌型衣原體多肽的分泌的活性分子的方法，其包含(a)將活性分子提供給衣原體培養物；(b)在適合所述活性分子對所述培養物發揮活性的條件下生長或孵育所述培養物一段時間；(c)在所述培養物中加入相應於權利要求49的多肽的引物對；(d)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(e)根據所述多核苷酸的存在與否來檢測分泌型衣原體多肽的存在。
69.用於表達分泌型沙眼衣原體多肽的質粒，其中所述質粒至少含有編碼權利要求49的多肽的多核苷酸。
70.權利要求69的質粒，其中所述多核苷酸還融合於一種報導基因。
71.權利要求70的質粒，其中於2000年12月13日保藏於C.N.C.M.的、保藏號為I-2593的載體被用於構建所述質粒。
72.一種重組革蘭氏陰性菌株，其中所述菌株由權利要求69的質粒轉化。
73.權利要求72的重組革蘭氏陰性菌株，其中所述菌株是一種志賀氏菌屬菌株。
74.一種預防或治療動物衣原體感染的方法，其包含施用有效量的一種純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種沙眼衣原體蛋白或其片段具有同源性，所述的組由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848組成。
75.權利要求74的方法，其中所述衣原體多肽是選自如下一組中的一或多種沙眼衣原體蛋白或其片段，所述的組由CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848組成。
76.權利要求74的方法，其中所述衣原體感染是沙眼衣原體感染。
77.權利要求74的方法，其中所述衣原體多肽是根據其被含有III型分泌途徑的革蘭氏陰性細菌菌株分泌而鑑別的。
78.權利要求74的方法，其中所述動物選自人、馬、牛、豬、山羊、綿羊、鳥、狗和貓。
79.權利要求74的方法，其中所述動物是人。
80.一種純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種鸚鵡熱衣原體蛋白或其片段具有同源性，所述的組由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058組成。
81.權利要求80的多肽，其中所述同源性衣原體多肽是鸚鵡熱衣原體蛋白。
82.權利要求80的多肽，其中所述多肽是根據其被革蘭氏陰性細菌菌株表達並被所述菌株的III型分泌途徑分泌而鑑別的。
83.權利要求80的多肽，其中所述衣原體多肽是選自如下一組中的一或多種鸚鵡熱衣原體或其片段，所述的組由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058組成。
84.權利要求80的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
85.權利要求80的多肽，其中所述多肽是依據一種用於鑑別由鸚鵡熱衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供至少含有編碼感興趣的多肽的多核苷酸的重組表達載體；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述多核苷酸表達產物的分泌；其中所述表達產物的分泌說明其相應於一種分泌型鸚鵡熱衣原體多肽。
86.權利要求85的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
87.權利要求80的多肽，其中所述多肽是依據一種用於鑑別由鸚鵡熱衣原體所分泌的多肽的方法而選擇的，所述方法包含(a)提供一種重組表達載體，所述重組表達載體至少包含融合於一種報導基因的編碼所述感興趣的多肽的DNA；(b)以所述重組載體轉化含有III型分泌途徑的革蘭氏陰性菌株；(c)在(b)中轉化的革蘭氏陰性菌株中表達該載體；以及(d)檢測所述報導基因表達產物的分泌；其中所述表達產物的分泌說明該融合的DNA至少含有一種相應於分泌型鸚鵡熱衣原體多肽的多核苷酸。
88.權利要求87的多肽，其中所述含有III型分泌途徑的革蘭氏陰性菌株是志賀氏菌屬菌株。
89.編碼至少一種權利要求80的多肽的純化的多核苷酸，或與所述編碼至少一種權利要求80的多肽的純化的多核苷酸在嚴格性條件下雜交的純化的多核苷酸。
90.權利要求89的純化的多核苷酸，其中所述多核苷酸是引物或探針。
91.一種免疫原性組合物，其至少包含權利要求80的多肽或其免疫原性片段。
92.一種針對鸚鵡熱衣原體感染的預防接種組合物，其中所述組合物包含至少一種權利要求80的多肽或其免疫原性片段以及一種藥物學可接受的載體。
93.權利要求92的預防接種組合物，其中所述感染是性傳播疾病。
94.一種抗鸚鵡熱衣原體的抗體，其中所述抗體針對權利要求80的多肽或其免疫原性片段。
95.一種診斷動物鸚鵡熱衣原體感染的方法，其中所述方法包含(a)提供一種疑似感染了鸚鵡熱衣原體的動物組織樣品；(b)將所述樣品與權利要求94的抗體相接觸；以及(c)檢測抗原抗體的複合；其中所述複合是表明所述動物存在鸚鵡熱衣原體感染的指徵。
96.一種預防或治療動物衣原體感染的方法，其包含給有需要的動物施用有效量的權利要求94的抗體或其免疫原性片段。
97.一種診斷動物鸚鵡熱衣原體感染的方法，其中所述方法包含(a)提供權利要求80的多肽或其免疫原性片段，任選地被標記；(b)將所述多肽或其免疫原性片段與所述動物的血清樣品相接觸；以及(c)檢測所述多肽或其免疫原性片段與血清樣品中含有的抗體之間形成的複合體；其中所述複合體是表明所述動物存在鸚鵡熱衣原體感染的指徵。
98.一種檢測動物體內的衣原體的方法，其中所述方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)在所述組織樣品中加入相應於權利要求80的多肽的引物對；(c)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(d)根據所述多核苷酸的存在與否來檢測衣原體的存在。
99.一種篩選抑制分泌型衣原體多肽的分泌的活性分子的方法，其包含(a)將活性分子提供給衣原體培養物；(b)在適合所述活性分子對所述培養物發揮活性的條件下生長或孵育所述培養物一段時間；(c)在所述培養物中加入相應於權利要求80的多肽的引物對；(d)擴增編碼相應於所選定的引物對的多肽的多核苷酸；以及(e)根據所述多核苷酸的存在與否來檢測分泌型衣原體多肽的存在。
100.一種表達分泌型鸚鵡熱衣原體多肽的質粒，其中所述質粒至少含有編碼權利要求80的多肽的DNA。
101.權利要求100的質粒，其中所述DNA還融合於一種報導基因。
102.權利要求101的質粒，其中於2000年12月13日保藏於C.N.C.M.的、保藏號為I-2593的載體被用於構建所述質粒。
103.一種重組革蘭氏陰性菌株，其中所述菌株由權利要求100的質粒轉化。
104.權利要求103的重組革蘭氏陰性菌株，其中所述菌株是一種志賀氏菌屬菌株。
105.一種預防或治療動物衣原體感染的方法，其包含施用有效量的一種純化的分泌型衣原體多肽，其中所述衣原體多肽與選自如下一組中的一或多種鸚鵡熱衣原體蛋白或其片段具有同源性，所述的組由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058組成。
106.權利要求105的方法，其中所述衣原體多肽是選自如下一組中的一或多種鸚鵡熱衣原體或其片段，所述的組由Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058組成。
107.權利要求105的方法，其中所述衣原體感染是鸚鵡熱衣原體感染。
108.權利要求105的方法，其中所述衣原體多肽是依據其被含有III型分泌途徑的革蘭氏陰性細菌菌株分泌而鑑別的。
109.權利要求105的方法，其中所述動物選自人、馬、牛、豬、山羊、綿羊、鳥、狗和貓。
110.權利要求105的方法，其中所述動物是人。
111.一種多核苷酸，其通過如下方法獲得，所述方法包含(a)將衣原體DNA片段與選自如下一組中的引物對相接觸，正向引物 反向引物SEQ ID NO133SEQ ID NO250SEQ ID NO134SEQ ID NO251SEQ ID NQ135SEQ ID NO252SEQ ID NO136SEQ ID NO253SEQ ID NO137SEQ ID NQ254SEQ ID NO138SEQ ID NO255SEQ ID NO139SEQ ID NO256SEQ ID NO140SEQ ID NO257SEQ ID NO141SEQ ID NO258SEQ ID NO142SEQ ID NO259SEQ ID NO143SEQ ID NO260SEQ ID NO144SEQ ID NO261SEQ ID NO145SEQ ID NO262SEQ ID NO146SEQ ID NO263SEQ ID NO147SEQ ID NO264SEQ ID NO148SEQ ID NO265SEQ ID NO149SEQ ID NO266SEQ ID NO150SEQ ID NO267SEQ ID NO151SEQ ID NO268SEQ ID NO152SEQ ID NO269SEQ ID NO153SEQ ID NO270SEQ ID NO154SEQ ID NO271SEQ ID NO155SEQ ID NO272SEQ ID NO156SEQ ID NO273SEQ ID NO157SEQ ID NO274SEQ ID NO158SEQ ID NO275SEQ ID NO159SEQ ID NO276SEQ ID NO160SEQ ID NO277SEQ ID NO161SEQ ID NO278SEQ ID NO162SEQ ID NO279SEQ ID NO163SEQ ID NO280SEQ ID NO164SEQ ID NO281SEQ ID NO165SEQ ID NO282SEQ ID NO166SEQ ID NO283SEQ ID NO167SEQ ID NO284SEQ ID NO168SEQ ID NO285SEQ ID NO169SEQ ID NO286SEQ ID NO170SEQ ID NO287SEQ ID NO171SEQ ID NO288SEQ ID NO172SEQ ID NO289SEQ ID NO173SEQ ID NO290SEQ ID NO174SEQ ID NO291SEQ ID NO175SEQ ID NO292SEQ ID NO176SEQ ID NO293SEQ ID NO177SEQ ID NO294SEQ ID NO178SEQ ID NO295SEQ ID NO179SEQ ID NO296SEQ ID NO180SEQ ID NO297SEQ ID NO181SEQ ID NO298SEQ ID NO182SEQ ID NO299SEQ ID NO183SEQ ID NO300SEQ ID NO184SEQ ID NO301SEQ ID NO185SEQ ID NO302SEQ ID NO186SEQ ID NO303SEQ ID NO187SEQ ID NO304SEQ ID NO188SEQ ID NO305SEQ ID NO189SEQ ID NO306SEQ ID NO190SEQ ID NO307SEQ ID NO191SEQ ID NO308SEQ ID NO192SEQ ID NO309SEQ ID NO193SEQ ID NO310SEQ ID NO194SEQ ID NO311SEQ ID NO195SEQ ID NO312SEQ ID NO196SEQ ID NO313SEQ ID NO197SEQ ID NO314SEQ ID NO198SEQ ID NO315SEQ ID NO199SEQ ID NO316SEQ ID NO200SEQ ID NO317SEQ ID NO201SEQ ID NO318SEQ ID NO202SEQ ID NO319SEQ ID NO203SEQ ID NO320SEQ ID NO204SEQ ID NO321SEQ ID NO205SEQ ID NO322SEQ ID NO206SEQ ID NO323SEQ ID NO207SEQ ID NO324SEQ ID NO208SEQ ID NO325SEQ ID NO209SEQ ID NO326SEQ ID NO210SEQ ID NO327SEQ ID NO211SEQ ID NO328SEQ ID NO212SEQ ID NO329SEQ ID NO213SEQ ID NO330SEQ ID NO214SEQ ID NO331SEQ ID NO215SEQ ID NO332SEQ ID NO216SEQ ID NO333SEQ ID NO217SEQ ID NO334SEQ ID NO218SEQ ID NO335SEQ ID NO219SEQ ID NO336SEQ ID NO220SEQ ID NO337SEQ ID NO221SEQ ID NO338SEQ ID NO222SEQ ID NO339SEQ ID NO223SEQ ID NO340SEQ ID NO224SEQ ID NO341SEQ ID NO225SEQ ID NO342SEQ ID NO226SEQ ID NO343SEQ ID NO227SEQ ID NO344SEQ ID NO228SEQ ID NO345SEQ ID NO229SEQ ID NO346SEQ ID NO230SEQ ID NO347SEQ ID NO231SEQ ID NO348SEQ ID NO232SEQ ID NO349SEQ ID NO233SEQ ID NO350SEQ ID NO234SEQ ID NO351SEQ ID NO235SEQ ID NO352SEQ ID NO236SEQ ID NO353SEQ ID NO237SEQ ID NO354SEQ ID NO238SEQ ID NO355SEQ ID NO239SEQ ID NO356SEQ ID NO240SEQ ID NO357SEQ ID NO241SEQ ID NO358SEQ ID NO242SEQ ID NO359SEQ ID NO243SEQ ID NO360SEQ ID NO244SEQ ID NO361SEQ ID NO245SEQ ID NO362SEQ ID NO246SEQ ID NO363SEQ ID NO247SEQ ID NO364SEQ ID NO248SEQ ID NO365SEQ ID NO249SEQ ID NO366SEQ ID NO367SEQ ID NO373SEQ ID NO368SEQ ID NO374SEQ ID NO369SEQ ID NO375SEQ ID NO370SEQ ID NO376SEQ ID NO371SEQ ID NO377SEQ ID NO372SEQ ID NO378以及(b)擴增編碼相應於所選定的引物對的多肽的多核苷酸。
112.一種檢測動物中的衣原體的方法，所述方法包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)加入與編碼衣原體多肽的多核苷酸在嚴格性條件下雜交的雜交探針；以及(c)根據所述多核苷酸的存在與否檢測衣原體的存在，其中所述衣原體多肽與選自肺炎衣原體蛋白、沙眼衣原體蛋白和鸚鵡熱衣原體蛋白中的一種蛋白或其片段具有同源性，所述肺炎衣原體蛋白選自CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058；所述沙眼衣原體蛋白選自CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848；以及所述鸚鵡熱衣原體蛋白選自Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058。
113.一種檢測動物體內的衣原體的方法，其包含(a)提供一種疑似感染了衣原體的動物組織樣品；(b)加入一種抗體，任選地被標記，所述抗體與衣原體多肽在適合形成複合體的條件下形成複合體；以及(c)根據所述多肽的存在與否檢測衣原體的存在，其中所述衣原體多肽與選自肺炎衣原體蛋白、沙眼衣原體蛋白和鸚鵡熱衣原體蛋白中的一種蛋白或其片段具有同源性，所述肺炎衣原體蛋白選自CPn0104、CPn0206、CPn0210、CPn0399、CPn0405、CPn0443、CPn0480、CPn0489、CPn0490、CPn0497、CPn0522、CPn0556、CPn0582、CPn0588、CPn0595、CPn0671、CPn0673、CPn0681、CPn0712、CPn0720、CPn0725、CPn0729、CPn0746、CPn0755、CPn0761、CPn0764、CPn0770、CPn0774、CPn0792、CPn0853、CPn0859、CPn0879、CPn0906、CPn0939、CPn1002、CPn1005、CPn1007、CPn1019、CPn1020、CPn1032和CPn1058；所述沙眼衣原體蛋白選自CT387、CT476、CT550、CT606.1、CT610、CT642、CT652.1、CT664、CT718、CT763、CT845和CT848；所述鸚鵡熱衣原體蛋白選自Psi0330、Psi0379、Psi0595、Psi0648、Psi0671、Psi0705、Psi0710、Psi0761、Psi0774、Psi1002、Psi1005、Psi1022和Psi1058。
全文摘要
本發明涉及分泌型衣原體多肽，其可被革蘭氏陰性細菌菌株表達並被所述菌株的III型分泌途徑的分泌。本發明還涉及編碼這些多肽的多核苷酸，並涉及這些分泌型衣原體多肽在治療和預防接種中的用途。
文檔編號C07K16/12GK1856505SQ200480010747
公開日2006年11月1日 申請日期2004年2月24日 優先權日2003年2月24日
發明者艾麗斯·多特裡－瓦爾薩特, 阿加特·敘布蒂－桑茲 申請人:巴斯德研究院, 國立科學研究中心